A heat inducible tyrosinase with distinct properties from Bacillus thuringiensis.

Abstract:

AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this purity was measured under different conditions to study its kinetic characterizations. The optimum pH and thermal stability of this enzyme were also determined. The results revealed that the tyrosinase from Bt has distinct properties compared with those from other sources. CONCLUSIONS:A heat-inducible tyrosinase of a wild strain of Bt was identified and partially characterized. SIGNIFICANCE AND IMPACT OF THE STUDY:The distinct properties of Bt tyrosinase are important to the application of Bt as a biology pesticide.

journal_name

Lett Appl Microbiol

authors

Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen P

doi

10.1111/j.1472-765X.2004.01599.x

subject

Has Abstract

pub_date

2004-01-01 00:00:00

pages

407-12

issue

5

eissn

0266-8254

issn

1472-765X

pii

LAM1599

journal_volume

39

pub_type

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