Abstract:
AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this purity was measured under different conditions to study its kinetic characterizations. The optimum pH and thermal stability of this enzyme were also determined. The results revealed that the tyrosinase from Bt has distinct properties compared with those from other sources. CONCLUSIONS:A heat-inducible tyrosinase of a wild strain of Bt was identified and partially characterized. SIGNIFICANCE AND IMPACT OF THE STUDY:The distinct properties of Bt tyrosinase are important to the application of Bt as a biology pesticide.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen Pdoi
10.1111/j.1472-765X.2004.01599.xsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
407-12issue
5eissn
0266-8254issn
1472-765Xpii
LAM1599journal_volume
39pub_type
杂志文章abstract:AIM:To investigate the viability, surface characteristics and ability of spores of a Geobacillus sp. isolated from a milk powder production line to adhere to stainless steel surfaces before and after a caustic (NaOH) wash used in clean-in-place regimes. METHODS AND RESULTS:Exposing sessile spores to 1% NaOH at 65°C fo...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02970.x
更新日期:2011-02-01 00:00:00
abstract:AIMS:Our goal was to develop a mathematical kinetic model to predict the sporicidal activity of glutaraldehyde, which is an active ingredient frequently used in commercial products employed for liquid disinfection and decontamination. METHODS AND RESULTS:We used our previously published data on spore inactivation by g...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02358.x
更新日期:2008-05-01 00:00:00
abstract::Natural enzyme inhibitors have been widely described in literature because of its pharmacological and cosmetic applications. Fungi found in caves represent a promising source of bioactive substances that are still little explored scientifically. Thus, the present work evaluated the presence of enzymatic modulators in ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13171
更新日期:2019-08-01 00:00:00
abstract:AIMS:To test the inhibitory activity of 2-nitro-1-propanol (2NPOH) against Salmonella Typhimurium, Escherichia coli O157:H7 and Enterococcus faecalis. METHODS AND RESULTS:Specific growth rates (h(-1)) of S. Typhimurium, E. coli O157:H7 and Ent. faecalis were determined during culture in tryptic soya broth (TSB) supple...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01613.x
更新日期:2004-01-01 00:00:00
abstract:AIMS: The primary goal of this study was to characterize the existence of a functional c-di-GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. METHODS AND RESULTS: A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03180.x
更新日期:2012-02-01 00:00:00
abstract:AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00928.x
更新日期:2001-06-01 00:00:00
abstract:UNLABELLED:Although little evidence existed to support that view, European countries and in particular France, have regarded echinoderms, including sea urchins, as low risk in terms of feacal contamination. It is hypothesized that the sea urchins mode of feeding, which is based on grazing and differs from bivalve mollu...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12524
更新日期:2016-02-01 00:00:00
abstract:UNLABELLED:In this study, we identified five strains isolated from soil and sediments able to degrade kraft lignin, aromatic dyes and lignin derivatives. Using 16S rRNA gene sequencing, the isolates were identified as Serratia sp. JHT01, Serratia liquefacien PT01, Pseudomonas chlororaphis PT02, Stenotrophomonas maltoph...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12581
更新日期:2016-07-01 00:00:00
abstract::Knowledge of the composition of the colonic microbiota is important for our understanding of how the balance of these microbes is influenced by diet and the environment, and which bacterial groups are important in maintaining gut health or promoting disease. Molecular methodologies have advanced our understanding of t...
journal_title:Letters in applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/j.1472-765X.2007.02129.x
更新日期:2007-04-01 00:00:00
abstract::Nisin, a bacteriocin produced by some strains of Lactococcus lactis, acts against foodborne pathogen Listeria monocytogenes. A single exposure of cells to nisin can generate nisin-resistant (Nisr) mutants, which may compromise the use of nisin in the food industry. The objective of this research was to compare the hea...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00708.x
更新日期:2000-03-01 00:00:00
abstract:AIMS:To evaluate a new dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) assay for detection of six sexually transmitted pathogens, including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum and Trichomonas vaginalis. METHO...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02618.x
更新日期:2009-07-01 00:00:00
abstract:AIMS:In this study, we compared different methods of colistin susceptibility testing, disc diffusion, agar dilution and Etest using a set of Enterobacteriaceae isolates that included colistin-resistant strains. METHODS AND RESULTS:Susceptibility of 200 clinical isolates of Enterobacteriaceae to colistin was tested to ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03145.x
更新日期:2011-11-01 00:00:00
abstract:AIMS:To measure the microbial removal capacity of a small-scale hydroponics wastewater treatment plant. METHODS AND RESULTS:Paired samples were taken from untreated, partly-treated and treated wastewater and analysed for faecal microbial indicators, i.e. coliforms, Escherichia coli, enterococci, Clostridium perfringen...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2005.01689.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To optimize the production of linolenic acid by disrupted mycelia of Mortierella isabellina. METHODS AND RESULTS:Effects of incubation conditions such as incubation time, pH of reaction mixture, concentration of Mg2+ or malate and incubation temperature on production of linolenic acid were studied. The production...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01291.x
更新日期:2003-01-01 00:00:00
abstract::The growth of pure cultures of Clostridium perfringens (ATCC 12922) and Cl. sporogenes (PA 3679) in five non-selective media, fluid thioglycollate medium (FTM), rapid perfringens medium (RPM), Columbia broth Malthus (CBM), reinforced clostridial medium (RCM) and lactose sulphite (LS), was monitored using conductance m...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.00330.x
更新日期:1997-01-01 00:00:00
abstract::The detection of foodborne viruses in bivalve molluscs is a challenging procedure in relation to low virus concentration and to the presence of significant RT-PCR inhibitors. The aim of this study was the development of an efficient direct extraction method for foodborne viral RNA from bivalve molluscs. Using Mengovir...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13065
更新日期:2018-11-01 00:00:00
abstract:AIMS:The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). METHODS AND RESULTS:Of the polymers tested, PE and PE + PEO did not exhibit an...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.01005.x
更新日期:2001-10-01 00:00:00
abstract::The objective of this research was to develop novel cellulose acetate (biopolymer) composite materials with an excellent antimicrobial activity by embedding molybdenum trioxide particles with unique high specific surface area. High surface area molybdenum trioxide particles were prepared from freshly precipitated moly...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12670
更新日期:2017-01-01 00:00:00
abstract:UNLABELLED:Industrial biotechnology uses microbiological cells to produce a wide range of products. While the organisms in question are well understood regarding their genetic and molecular properties, less is known about their mechanical properties. Previous work has established a testing procedure for single Saccharo...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12468
更新日期:2015-10-01 00:00:00
abstract:AIMS:To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. METHODS AND RESULTS:Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultur...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02299.x
更新日期:2008-02-01 00:00:00
abstract:AIMS:To investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in modified Pine's medium (Pine et al. 1989). METHODS AND RESULTS:Modified Pine's medium containing 0, 0.044, 0.088 or 0.176 g l(-1) ferric ammonium citrate, and 0 or 1 mM ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01237.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS:Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x1...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2004.01474.x
更新日期:2004-01-01 00:00:00
abstract:AIM:The aim of this work was to evaluate the effect of Planococcus ficus infection in red wine grapes on Aspergillus section Nigri and ochratoxin A (OTA) contamination. METHODS AND RESULTS:During 2006/2007 and 2008/2009 vintages, Merlot, Malbec and Cabernet Sauvignon varieties divided into two categories of grape samp...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2010.02884.x
更新日期:2010-08-01 00:00:00
abstract:AIMS:To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. METHODS AND RESULTS:A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 diff...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02507.x
更新日期:2009-02-01 00:00:00
abstract:AIMS:A commercial biochemical panel ID kit was used to identify presumptive enterococci isolates of veterinary or agricultural origin obtained during different steps of culture. METHODS AND RESULTS:Fifty isolates identified as enterococci using a genus PCR assay were tested for genus and species identification using t...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01302.x
更新日期:2003-01-01 00:00:00
abstract::The objective of this study was to undertake a microbiological survey of foods, animal faeces and wastewater samples for Clostridium difficile, and determine the genotypes and antimicrobial susceptibilities of isolates. A total of 211 samples were tested for C. difficile using culture methods. Thirteen toxigenic C. di...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13238
更新日期:2020-01-01 00:00:00
abstract::Candida-associated denture stomatitis presents as erythema of the palatal mucosa and is caused by biofilms containing the fungus Candida albicans that co-reside with oral bacteria on the denture-fitting surface. This study aimed to assess the effect of several frequently encountered oral bacteria on the expression of ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13145
更新日期:2019-04-01 00:00:00
abstract:AIMS:To apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to pulsed-plasma gas discharges (PPGD). METHODS AND RESULTS:The fluorescent redox probe 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was used for enumerati...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02268.x
更新日期:2008-01-01 00:00:00
abstract::Species of Aspergillus section Nigri are not easily distinguished by traditional morphological techniques, and typically are identified by DNA sequencing methods. We developed four PCR primers to distinguish between Aspergillus niger, Aspergillus welwitschiae, Aspergillus carbonarius and Aspergillus tubingensis, based...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12358
更新日期:2015-02-01 00:00:00
abstract:AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00950.x
更新日期:2001-07-01 00:00:00