Abstract:
:Knowledge of the composition of the colonic microbiota is important for our understanding of how the balance of these microbes is influenced by diet and the environment, and which bacterial groups are important in maintaining gut health or promoting disease. Molecular methodologies have advanced our understanding of the composition and diversity of the colonic microbiota. Importantly, however, it is the continued isolation of bacterial representatives of key groups that offers the best opportunity to conduct detailed metabolic and functional studies. This also permits bacterial genome sequencing which will accelerate the linkage to functionality. Obtaining new human colonic bacterial isolates can be challenging, because most of these are strict anaerobes and many have rather exact nutritional and physical requirements. Despite this many new species are being isolated and described that occupy distinct niches in the colonic microbial community. This review focuses on these under-studied yet important gut anaerobes.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Duncan SH,Louis P,Flint HJdoi
10.1111/j.1472-765X.2007.02129.xsubject
Has Abstractpub_date
2007-04-01 00:00:00pages
343-50issue
4eissn
0266-8254issn
1472-765Xpii
LAM2129journal_volume
44pub_type
杂志文章,评审abstract::A method for developing a single-transposon-insertion mutant from a double-insertion mutant in Rhizobium is described. An exopolysaccharide (EPS)-defective mutant containing two Tn5-lacZ insertions was complemented with cloned wild-type DNA for EPS synthesis. One of the Tn5-lacZ insertions from the mutant was transfer...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1994.tb00927.x
更新日期:1994-09-01 00:00:00
abstract:AIMS:The objective was to demonstrate the size of silver particles produced by the filamentous fungus Phoma sp.3.2883 via adsorption and accumulation, and to confirm that this silver was in a reduced state. METHODS AND RESULTS:Mycelium was freeze-dried and then shake-cultured in a silver nitrate solution. It was found...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01348.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:Species-specific PCR was applied to identify Lactobacillus brevis and the sensitivity and the specificity of the protocol were determined. METHODS AND RESULTS:Strains of Lact. brevis obtained from foods, particularly dairy products, and various strain collections, were identified by PCR using primers which amplif...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.01014.x
更新日期:2001-11-01 00:00:00
abstract:AIMS:Alpha-galactosidase is applied in food and feed industries for hydrolysing raffinose series oligosaccharides (RO) that are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. The objective of the current work was to develop an optimal culture medium for the production of al...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02173.x
更新日期:2007-08-01 00:00:00
abstract::In a crossover study, volunteers consumed a diet supplemented with either wheat or rye bread. Levels of faecal mucus were determined and the adhesion of five selected probiotics to immobilized intestinal mucus, was assessed. No significant difference in the concentration of faecal mucus or adhesion of the probiotics t...
journal_title:Letters in applied microbiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1472-765x.2000.00758.x
更新日期:2000-07-01 00:00:00
abstract:AIMS:To evaluate the accuracy of pyrosequencing for the description of Phytophthora communities in terms of taxa identification and risk of assignment for false Molecular Operational Taxonomic Units (MOTUs). METHODS AND RESULTS:Pyrosequencing of Internal Transcribed Spacer 1 (ITS1) amplicons was used to describe the s...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2012.03310.x
更新日期:2012-11-01 00:00:00
abstract:AIMS: To develop a new instrument of great versatility for recovering micro-organisms from all types of food samples and to compare the effects with existing sample preparation methods. METHODS AND RESULTS: To detach micro-organisms from large-size unbroken food samples such as apples, carrots, potatoes and tomatoes w...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2012.03289.x
更新日期:2012-09-01 00:00:00
abstract:AIMS: To show that application of copper hydroxide citrus sprays mixed with field source water (possibly contaminated) will not support Escherichia coli on plant surfaces. Environmental stresses of transient phyllosphere bacteria and presence of copper will eradicate these bacteria before harvest. METHODS AND RESULTS...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03179.x
更新日期:2012-02-01 00:00:00
abstract:UNLABELLED:AIMIS: Draft WHO guidance is likely to suggest a calculated 95th percentile method, rather than percentage exceedence, in assessing compliance of bathing waters with microbiological standards. This study set out to determine whether this was an appropriate development. METHODS AND RESULTS:A series of Monte ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01081.x
更新日期:2002-01-01 00:00:00
abstract:UNLABELLED:Bioconversion of biomass, particularly crop wastes, into biofuels is being developed as an alternative approach in meeting the high energy demand. In this study, a thermophilic bacterial strain BY-3 that exhibits cellulolytic potential was isolated from faecal samples of Tibetan pigs; this strain was identif...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12276
更新日期:2014-09-01 00:00:00
abstract::This study evaluated the performance of CPS ID2 (bioMérieux) compared to that of conventionally used selective agar for the identification of bacteria responsible for urinary tract infections. This medium detects bacterial enzymes using chromogenic substrates. Two hundred and nineteen samples of urine were tested in o...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb00450.x
更新日期:1995-05-01 00:00:00
abstract::A wild type micro-organism producing antibacterial substances has been isolated from a Chinese fermented soybean seasoning and identified as Bacillus subtilis. A crude antibacterial preparation (CABP) was obtained by ammonium sulphate precipitation. Isoelectric focusing assay revealed at least four antimicrobial compo...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00545.x
更新日期:1999-05-01 00:00:00
abstract:AIMS:In this study, we compared different methods of colistin susceptibility testing, disc diffusion, agar dilution and Etest using a set of Enterobacteriaceae isolates that included colistin-resistant strains. METHODS AND RESULTS:Susceptibility of 200 clinical isolates of Enterobacteriaceae to colistin was tested to ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03145.x
更新日期:2011-11-01 00:00:00
abstract:AIMS:Natural and synthetic antimicrobial peptides (AMPs) are of increasing interest as potential resistance conferring elements in plants against pathogen infection. The efficacy of AMPs against pathogens is prescreened by in vitro assays, and promising AMP candidates are introduced as transgenes into plants. As in vit...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2012.03244.x
更新日期:2012-06-01 00:00:00
abstract:AIM:The main aim of this study was to investigate a possible connection between the Salmonella content in sewage sludge and human cases of salmonellosis. An additional aim was to survey the antimicrobial resistance situation in Salmonella isolated from Swedish sewage sludge. METHODS AND RESULTS:The Salmonella strains ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01911.x
更新日期:2006-07-01 00:00:00
abstract:AIMS:This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS:A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2008.02421.x
更新日期:2008-10-01 00:00:00
abstract::Fifty-five isolates of nine species and two species varieties (5 isolates each) of the Aspergillus niger group were tested for progesterone transformation. Generally, all isolates of the different species transformed progesterone to 21-hydroxyprogesterone. In addition, the isolates of seven species have the capacity t...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb00438.x
更新日期:1995-04-01 00:00:00
abstract:AIMS:The aim of this study was to determine the prevalence and proportions of opportunistic pathogens harboured on orthodontic retainers. METHODS AND RESULTS:First, Staphylococcus spp. and Candida spp. were isolated from the retainer's inner surface and from other mucosal surfaces of the subject's mouth by routine bac...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03031.x
更新日期:2011-05-01 00:00:00
abstract::Results of biocide efficacy testing on laboratory-generated microbial biofilms containing Legionella bozemanii is presented. These show that chlorine is effective at recommended concentrations in cooling towers; at least 48 h contact between free chlorine and the biofilm is necessary. Of five commercial biocides teste...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1993.tb00384.x
更新日期:1993-10-01 00:00:00
abstract:AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02863.x
更新日期:2010-07-01 00:00:00
abstract::Pinellia ternata is a traditional Chinese herb that has been shown to suffer from continuous cropping. Deterioration in continuous P. ternata cropping soils and the effect of alteration of crop rotation on the soil were evaluated by using Illumina high-throughput sequencing technology. Soil physicochemical properties ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13135
更新日期:2019-06-01 00:00:00
abstract:AIMS:To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS:Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x1...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2004.01474.x
更新日期:2004-01-01 00:00:00
abstract::The use of energy dispersive analysis of X-rays (EDAX) to identify and quantify the presence of chlorhexidine diacetate (CHA) within Saccharomyces cerevisiae cells was examined. Chlorine was used as the elemental marker tag. Saccharomyces cerevisiae cells exposed to 1000 micrograms ml-1 CHA took up increasing amounts ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb01319.x
更新日期:1995-06-01 00:00:00
abstract:AIMS:This study assessed the plant growth-promoting ability of the bacterial strains Ochrobactrum intermedium (isolate CrT-1) and Bacillus cereus (isolate S-6). METHODS AND RESULTS:Two chromium resistant bacterial strains isolated from chromium-contaminated wastewater and soils were identified as O. intermedium CrT-1 ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01977.x
更新日期:2006-10-01 00:00:00
abstract:AIMS:To evaluate mycobiota and aflatoxins B(1) (AFB(1)), B(2) (AFB(2)), G(1) (AFG(1)), G(2) (AFG(2)) and fumonisin B(1) (FB(1)) contamination in different malted barley types and brands and brewer's grain collected from a major Argentinean brewery. METHODS AND RESULTS:Total fungal counts were performed using the plate...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03157.x
更新日期:2011-12-01 00:00:00
abstract:AIMS:The study was carried out to evaluate the use of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) as a method for the identification of lactobacilli isolated from meat products. METHODS AND RESULTS:RAPD-PCR with primers M13 and D8635 was applied to the identification and intraspecific diffe...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00939.x
更新日期:2001-07-01 00:00:00
abstract::Iron availability plays an important role in the virulence of micro-organisms, which develops different systems for iron acquisition. The expression of genes involved in iron uptake systems is usually regulated by Fur, a transcriptional regulator. Plesiomonas shigelloides is a Gram-negative food- and water-borne enter...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12858
更新日期:2018-04-01 00:00:00
abstract:AIMS:The aim of this work was to optimize the production of a novel antimaralial menisporopsin A by the seed fungus Menisporopsis theobromae BCC 4162. METHODS AND RESULTS:Fungal cultures were grown in shake flasks at 25 degrees C in the basal medium with varying carbon and nitrogen sources, aeration rates and initial ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01994.x
更新日期:2006-11-01 00:00:00
abstract:AIM:To evaluate the effect of starvation, heat, cold, acid, alkaline, chlorine and ethanol stresses on the resistance of Enterobacter sakazakii in powdered infant milk formula (PIMF) towards gamma radiation. METHODS AND RESULTS:Stressed cells of E. sakazakii ATCC 51329 and four other food isolate strains were mixed in...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02388.x
更新日期:2008-08-01 00:00:00
abstract:AIMS:To improve the production of sweet-tasting protein brazzein in Lactococcus lactis using controlled fermentation conditions. METHODS AND RESULTS:The nisin-controlled expression system was used for brazzein expression. The concentration of nisin for induction and the optical density (OD) at induction were therefore...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02297.x
更新日期:2008-02-01 00:00:00