Isolation, partial purification and characterization of a bacteriocin produced by a newly isolated Bacillus subtilis strain.

abstract：:Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serot...

journal_title：Letters in applied microbiology

authors： Black SF,Gray DI,Fenlon DR,Kroll RG

更新日期：1995-03-01 00:00:00

abstract：AIMS:To investigate whether intranasal Lactobacillus administration protects host animals from influenza virus (IFV) infection by enhancing respiratory immune responses in a mouse model. METHODS AND RESULTS:After 3 days of intranasal exposure to Lactobacillus rhamnosus GG (LGG), BALB/c mice were infected with IFV A/PR...

journal_title：Letters in applied microbiology

authors： Harata G,He F,Hiruta N,Kawase M,Kubota A,Hiramatsu M,Yausi H

更新日期：2010-06-01 00:00:00

abstract：:Candida-associated denture stomatitis presents as erythema of the palatal mucosa and is caused by biofilms containing the fungus Candida albicans that co-reside with oral bacteria on the denture-fitting surface. This study aimed to assess the effect of several frequently encountered oral bacteria on the expression of ...

journal_title：Letters in applied microbiology

authors： Morse DJ,Wilson MJ,Wei X,Bradshaw DJ,Lewis MAO,Williams DW

更新日期：2019-04-01 00:00:00

abstract：AIMS:From a survey of submerged culture of edible mushrooms, a high pigment-producing fungus Paecilomyces sinclairii was selected and its optimal culture conditions investigated. METHODS AND RESULTS:The optimal culture conditions for pigment production were as follows: inoculum age, 3 d; temperature, 25 degrees C; ini...

journal_title：Letters in applied microbiology

authors： Cho YJ,Park JP,Hwang HJ,Kim SW,Choi JW,Yun JW

更新日期：2002-01-01 00:00:00

abstract：AIMS:In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. METHODS AND RESULTS:Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite...

journal_title：Letters in applied microbiology

authors： Gunaydin E,Eyigor A,Carli KT

更新日期：2007-01-01 00:00:00

abstract：:Sixteen Bifidobacterium isolates from the human gastrointestinal tract were assayed for susceptibility to 44 antibiotics by soft agar overlay disc diffusion on TPY agar. Five isolates (3/7 B. bifidum and 2/3 B. breve) exhibited atypical antibiotic susceptibility profiles. Poor growth in the agar overlay accounted for ...

journal_title：Letters in applied microbiology

authors： Charteris WP,Kelly PM,Morelli L,Collins JK

更新日期：1998-05-01 00:00:00

abstract：AIMS:The aim of this study was to elucidate if the need for iron for Escherichia coli to remain cultivable in a poorly nutritive medium such as the drinking water uses the iron transport system via the siderophores. METHODS AND RESULTS:Environmental strains of E. coli (isolated from a drinking water network), referenc...

journal_title：Letters in applied microbiology

authors： Grandjean D,Jorand F,Guilloteau H,Block JC

更新日期：2006-07-01 00:00:00

abstract：:Candida albicans is an opportunistic fungus, which causes vulvovaginal candidiasis (VVC). The aim of this study was to evaluate Mrr2 mutation and its expression levels and Candida drug resistance 1 (Cdr1) in C. albicans associated with fluconazole (FCA) resistance. We identified 80 isolates of C. albicans from 155 vag...

journal_title：Letters in applied microbiology

authors： Feng W,Yang J,Ji Y,Xi Z,Yang L,Zhu X,Ma Y

更新日期：2020-02-01 00:00:00

abstract：AIMS:To compare the bacteriostatic and bactericidal activity of 13 chemotyped essential oils (EO) on 65 bacteria with varying sensitivity to antibiotics. METHODS AND RESULTS:Fifty-five bacterial strains were tested with two methods used for evaluation of antimicrobial activity (CLSI recommendations): the agar dilution...

journal_title：Letters in applied microbiology

authors： Mayaud L,Carricajo A,Zhiri A,Aubert G

更新日期：2008-09-01 00:00:00

abstract：:The physical effects of high-intensity pulsed electric fields (PEF) on the inactivation of diarrhoeagenic Bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (TEM). The levels of PEF-induced microbial cell death were determined by enumeration on tryptone soy yeast ex...

journal_title：Letters in applied microbiology

authors： Rowan NJ,MacGregor SJ,Anderson JG,Fouracre RA,Farish O

更新日期：2000-08-01 00:00:00

abstract：AIMS: The objective of this study was to inactivate Bacillus cereus spores in sikhye using a modified tyndallization process involving injection with carbon dioxide (CO₂). METHODS AND RESULTS:Heat tolerance of B. cereus spores in tryptic soy broth and sikhye was evaluated. The D(95°C) values of the B. cereus spores we...

journal_title：Letters in applied microbiology

authors： Kim H,Kim H,Bang J,Kim Y,Beuchat LR,Ryu JH

更新日期：2012-09-01 00:00:00

abstract：AIM:To identify the gene that encodes nigrescin, a bacteriocin produced by Prevotella nigrescens ATCC 25261. METHODS AND RESULTS:Each open reading frame (ORF) of the nig gene cluster (nigA, nigB, nigC and nigD) was transferred into an expression vector. The recombinant proteins encoded by nigA, nigB, nigC and nigD wer...

journal_title：Letters in applied microbiology

authors： Teanpaisan R,Narawatthana S,Utarabhand P

更新日期：2009-09-01 00:00:00

abstract：AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this pu...

journal_title：Letters in applied microbiology

authors： Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen P

更新日期：2004-01-01 00:00:00

abstract：AIMS:Genista saharae, indigenous of Sahara, is a spontaneous shrub that plays an important ecological role for the preservation and fertility of poor and eroded soils. This legume has not been examined for its root nodule bacteria. The taxonomic diversity of bacteria from root nodules of G. saharae growing in the infra...

journal_title：Letters in applied microbiology

authors： Mahdhi M,Nzoué A,Gueye F,Merabet C,de Lajudie P,Mars M

更新日期：2007-12-01 00:00:00

abstract：:The fate of Salmonella enteritidis PT4 in home-made mayonnaise prepared with citric acid solution (citric acid concentration of > or = 4.98% (w/v)) was investigated. It was found that pH of mayonnaise is closely related to the ratio of egg yolk to citric acid, and the inactivation rate of the micro-organisms increases...

journal_title：Letters in applied microbiology

authors： Xiong R,Xie G,Edmondson AS

更新日期：1999-01-01 00:00:00

abstract：AIMS:To identify and compare microbiota in Chinese liquor Daqu, which were produced in the different regions using different production process. METHODS AND RESULTS:The DNA exacted from Daqu samples was used as a template for PCR with universal primers of 16S rRNA, 26S rRNA and 18S rRNA, respectively. The amplicons we...

journal_title：Letters in applied microbiology

authors： Wang HY,Gao YB,Fan QW,Xu Y

更新日期：2011-08-01 00:00:00

abstract：AIMS:This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples. METHODS AND RESULTS:A total of 285 stool samples were inoculated onto Chr...

journal_title：Letters in applied microbiology

authors： Asir K,Wilkinson K,Perry JD,Reed RH,Gould FK

更新日期：2009-02-01 00:00:00

abstract：AIMS:The aim of this study was to detect and quantify DNA of the ochratoxinogenic fungus Aspergillus ochraceus in green coffee and to compare the results with the ochratoxin A content of naturally contaminated samples. METHODS AND RESULTS:A DNA extraction protocol based on a combination of ultrasonification and a comm...

journal_title：Letters in applied microbiology

authors： Schmidt H,Bannier M,Vogel RF,Niessen L

更新日期：2004-01-01 00:00:00

abstract：:Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram-positive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which re...

journal_title：Letters in applied microbiology

authors： McCormick JK,Klaenhammer TR,Stiles ME

更新日期：1999-07-01 00:00:00

abstract：:The ability of chemotherapeutic agents to affect the growth of common bacterial pathogens and the relationship between the effects of chemotherapeutics and antimicrobials is largely unknown. The purpose of this study was to describe the susceptibility of canine bacterial isolates to chemotherapeutic agents and to comp...

journal_title：Letters in applied microbiology

authors： Campbell O,Gagnon J,Rubin JE

更新日期：2019-11-01 00:00:00

abstract：:In this study, a total of 172 putative omega-3 producers were isolated from 28 sediment samples from the Arabian Gulf employing a selective isolation procedure using marine agar containing 0·1% triphenyl tetrazolium chloride (TTC). Out of these 172 isolates, 19 isolates produced eicosapentaenoic acid (EPA) as confirme...

journal_title：Letters in applied microbiology

authors： Alagarsamy S,Sabeena Farvin KH,Fakhraldeen S,Kooramattom MR,Al-Yamani F

更新日期：2019-08-01 00:00:00

abstract：AIMS:The aim of this study was to identify and determine the diversity, occurrence and distribution of fungi in water used at a haemodialysis centre. METHODS AND RESULTS:Samples in the hydraulic circuit for the distribution of the water, dialysate samples and samples of sterilization solution from dialysers were colle...

journal_title：Letters in applied microbiology

authors： Pires-Gonçalves RH,Sartori FG,Montanari LB,Zaia JE,Melhem MS,Mendes-Giannini MJ,Martins CH

更新日期：2008-05-01 00:00:00

abstract：AIMS:To determine the effect of interacting conditions of water activity (aw, 0.99-0.85), temperature (15, 25 degrees C) and time (40 days) on growth and production of the mycotoxins deoxynivalenol (DON) and nivalenol (NIV) by Fusarium culmorum on a wheat-based agar medium. METHODS AND RESULTS:Fusarium culmorum grew o...

journal_title：Letters in applied microbiology

authors： Hope R,Magan N

更新日期：2003-01-01 00:00:00

abstract：:A new ultrasonic apparatus operating at a frequency of 40 kHz was developed to dislodge biofilms from food processing equipment in order to assess the effectiveness of cleaning protocols. Sonication conditions to remove biofilms and quantification by ATP-bioluminescence are described. An industrial meat process was de...

journal_title：Letters in applied microbiology

authors： Oulahal-Lagsir N,Martial-Gros A,Boistier E,Blum LJ,Bonneau M

更新日期：2000-01-01 00:00:00

abstract：AIMS:The purpose of this study was to determine if DNA polymorphisms generated by RAPD-PCR could be used to characterize Group B streptococci (GBS) for epidemiological purposes. METHODS AND RESULTS:30 unrelated, previously serotyped strains were analysed by RAPD-PCR using two 10-mer primers (5' TGCGAGAGTC 3' and 5' AG...

journal_title：Letters in applied microbiology

authors： Zhang GW,Kotiw M,Daggard G

更新日期：2002-01-01 00:00:00

abstract：AIMS:The use of randomly amplified polymorphic DNA (RAPD)-PCR fingerprinting and plasmid profiles to determine at the strain level, the similarity of Lactococcus lactis isolates obtained during sampling of traditional cheeses and to verify its correspondence to the selected phenotypic characteristics. METHODS AND RESU...

journal_title：Letters in applied microbiology

authors： de la Plaza M,Rodríguez A,Fernández de Palencia P,Martínez-Cuesta MC,Peláez C,Requena T

更新日期：2006-12-01 00:00:00

abstract：:Three groups of substances were identified which inhibit the bactericidal activity of bacitracin. Beside two divalent metals, Mg2+ and Ca2+, and a metabolite with chelating properties, citrate, the most pronounced effect was observed with pyrophosphate. Metals probably prevent access of bacitracin to the lipid carrier...

journal_title：Letters in applied microbiology

authors： Podlesek Z,Comino A

更新日期：1994-08-01 00:00:00

abstract：AIMS:The effect of high organic loading rate (OLR) on the physical characteristics of aerobic granules was studied. METHODS AND RESULTS:Two column-type sequential aerobic sludge blanket reactors were fed with either glucose or acetate as the main carbon source, and the OLR was gradually raised from 6 to 9, 12 and 15 k...

journal_title：Letters in applied microbiology

authors： Moy BY,Tay JH,Toh SK,Liu Y,Tay ST

更新日期：2002-01-01 00:00:00

abstract：:Many strains of Pseudomonas fluorescens show potential for biological control of phytopathogens especially root pathogens. In taxonomic terms, several of them are indeed P. fluorescens sensu stricto, while others belong in fact to neighbouring species of the 'P. fluorescens' complex or to ill-defined related species w...

journal_title：Letters in applied microbiology

authors： Couillerot O,Prigent-Combaret C,Caballero-Mellado J,Moënne-Loccoz Y

更新日期：2009-05-01 00:00:00

abstract：AIMS:The study aim was to determine the presence of total and faecal coliforms on kitchen surfaces, in tap water and on the hands of caregivers in households on both sides of the US-Mexico border. METHODS AND RESULTS:Samples were collected in 135 randomly selected households in Ciudad Juarez, Mexico, and El Paso, Texa...

journal_title：Letters in applied microbiology

authors： Carrasco L,Mena KD,Mota LC,Ortiz M,Behravesh CB,Gibbs SG,Bristol JR,Mayberry L,Cardenas VM

更新日期：2008-06-01 00:00:00