Vapour-phase hydrogen peroxide inactivates Yersinia pestis dried on polymers, steel, and glass surfaces.

Abstract:

AIMS:This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS:A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide fumigation for a contact time of 2 h. A significant reduction in the log10 CFU of Y. pestis on all 10 materials was observed between the controls evaluated after a 1 h drying time and unexposed controls evaluated after the decontamination run. Qualitative growth assessment showed that vapour-phase hydrogen peroxide exposure inactivated Y. pestis on all replicates of the 10 test materials as well as biological indicators up to 7 days postexposure. CONCLUSIONS:Virulent Y. pestis CO92 is inactivated on polymers, steel, and glass surfaces when exposed to vapour-phase hydrogen peroxide without observable physical damage to the test materials. SIGNIFICANCE AND IMPACT OF THE STUDY:This study provides information for using vapour-phase hydrogen peroxide as a practical process for the decontamination of virulent Y. pestis in circumstances where time-dependent attenuation/inactivation orliquid/heat decontamination may not be the most suitable approach.

journal_name

Lett Appl Microbiol

authors

Rogers JV,Richter WR,Shaw MQ,Choi YW

doi

10.1111/j.1472-765x.2008.02421.x

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

279-85

issue

4

eissn

0266-8254

issn

1472-765X

journal_volume

47

pub_type

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