Detection and characterization of the ferric uptake regulator (fur) gene in Plesiomonas shigelloides.

abstract：:Emulsions of the fatty acids linoleic (C18:2 n-6), alpha-linolenic (C18:3 n-3) and arachidonic acid (C20:4 n-6) were incubated for 4 h under anaerobic conditions with human faecal suspensions. Linoleic acid was significantly decreased (P < 0.001) and there was a significant rise (P < 0.05) in its hydrogenation product...

journal_title：Letters in applied microbiology

authors： Howard FA,Henderson C

更新日期：1999-09-01 00:00:00

abstract：:This study evaluated the performance of CPS ID2 (bioMérieux) compared to that of conventionally used selective agar for the identification of bacteria responsible for urinary tract infections. This medium detects bacterial enzymes using chromogenic substrates. Two hundred and nineteen samples of urine were tested in o...

journal_title：Letters in applied microbiology

authors： Willinger B,Manafi M

更新日期：1995-05-01 00:00:00

abstract：:Flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degrees C, 5 min) and nisin treatments (0.1 and 1.0 microgram ml-1) of La...

journal_title：Letters in applied microbiology

authors： Ueckert JE,Nebe von-Caron G,Bos AP,ter Steeg PF

更新日期：1997-10-01 00:00:00

abstract：AIMS:To investigate interactions between rumen protozoa and Shiga toxin-producing Escherichia coli (STEC) and to ascertain whether it is likely that rumen protozoa act as ruminant hosts for STEC. METHODS AND RESULTS:The presence of stx genes in different microbial fractions recovered from cattle and sheep rumen conten...

journal_title：Letters in applied microbiology

authors： Burow LC,Gobius KS,Vanselow BA,Klieve AV

更新日期：2005-01-01 00:00:00

abstract：:Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

journal_title：Letters in applied microbiology

authors： Wang SQ,Wang T,Liu JF,Deng L,Wang F

更新日期：2018-11-01 00:00:00

abstract：:Cow's milk was inoculated with ca 10(3) and 10(7) cfu ml-1 Escherichia coli O157:H7. After fermentation at 42 degrees C for 0-5 h, the yoghurt was stored at 4 degrees C. Two kinds of yoghurt were used: traditional yoghurt (TY), made with Streptococcus thermophilus and Lactobacillus bulgaricus starter cultures, and 'bi...

journal_title：Letters in applied microbiology

authors： Massa S,Altieri C,Quaranta V,De Pace R

更新日期：1997-05-01 00:00:00

abstract：AIMS:To test the inhibitory activity of 2-nitro-1-propanol (2NPOH) against Salmonella Typhimurium, Escherichia coli O157:H7 and Enterococcus faecalis. METHODS AND RESULTS:Specific growth rates (h(-1)) of S. Typhimurium, E. coli O157:H7 and Ent. faecalis were determined during culture in tryptic soya broth (TSB) supple...

journal_title：Letters in applied microbiology

authors： Jung YS,Anderson RC,Callaway TR,Edrington TS,Genovese KJ,Harvey RB,Poole TL,Nisbet DJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...

journal_title：Letters in applied microbiology

authors： Sun J,Hu XL,Le GW,Shi YH

更新日期：2010-03-01 00:00:00

abstract：UNLABELLED:This study reports the development of a loop-mediated isothermal amplification (LAMP) reaction for the detection of Pythium myriotylum. The primer set targeting the ITS sequence of P. myriotylum worked most efficiently at 60°C and allowed the detection of P. myriotylum DNA within 30 min by fluorescence monit...

journal_title：Letters in applied microbiology

authors： Fukuta S,Takahashi R,Kuroyanagi S,Ishiguro Y,Miyake N,Nagai H,Suzuki H,Tsuji T,Hashizume F,Watanabe H,Kageyama K

更新日期：2014-07-01 00:00:00

abstract：UNLABELLED:Assessment of antibacterial as well as antiherpes virus activity of sulfonoquinovosyldiacylglyceride (SQDG), a glycolipid, isolated from the leaves of Azadirachta indica has been described. Antimicrobial activity was evaluated against Gram-positive, Gram-negative bacteria and herpes simplex virus. SQDG showe...

journal_title：Letters in applied microbiology

authors： Bharitkar YP,Bathini S,Ojha D,Ghosh S,Mukherjee H,Kuotsu K,Chattopadhyay D,Mondal NB

更新日期：2014-02-01 00:00:00

abstract：AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...

journal_title：Letters in applied microbiology

authors： Park JP,Kim SW,Hwang HJ,Yun JW

更新日期：2001-07-01 00:00:00

abstract：:The effect of surfactant type and concentration on a bland soap formulation's ability to remove bacteria from hands remains largely unstudied. Several combinations of surfactants and water were combined to test bacterial removal efficacy using a hand-washing device (two pieces of pig skin and a mechanical motor) to si...

journal_title：Letters in applied microbiology

authors： Jensen DA,Rogers MA,Schaffner DW

更新日期：2017-10-01 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a worldwide concern. Cattle are their main reservoir and may contaminate watercourses through manure. We characterized a collection of 38 STEC O157:H7 strains isolated from surface water in feedlots areas (puddles inside pens formed after the rainfall or by spil...

journal_title：Letters in applied microbiology

authors： Tanaro JD,Pianciola LA,D'Astek BA,Piaggio MC,Mazzeo ML,Zolezzi G,Rivas M

更新日期：2018-06-01 00:00:00

abstract：UNLABELLED:Blue light (470 nm) LED antimicrobial properties were studied alone against bacteria and with or without the food grade photosensitizer, erythrosine (ERY) against filamentous fungi. Leuconostoc mesenteroides (LM), Bacillus atrophaeus (BA) or Pseudomonas aeruginosa (PA) aliquots were exposed on nutrient agar ...

journal_title：Letters in applied microbiology

authors： De Lucca AJ,Carter-Wientjes C,Williams KA,Bhatnagar D

更新日期：2012-12-01 00:00:00

abstract：:The recent rise in the awareness of the occurrence of toxic cyanobacterial blooms in aquatic environments, with associated human health problems and animal deaths, has increased the need for rapid, reliable and sensitive methods of determining cyanobacterial toxicity. A luminescent bacterial toxicity test was assessed...

journal_title：Letters in applied microbiology

authors： Lawton LA,Campbell DL,Beattie KA,Codd GA

更新日期：1990-10-01 00:00:00

abstract：AIMS:The study aim was to determine the presence of total and faecal coliforms on kitchen surfaces, in tap water and on the hands of caregivers in households on both sides of the US-Mexico border. METHODS AND RESULTS:Samples were collected in 135 randomly selected households in Ciudad Juarez, Mexico, and El Paso, Texa...

journal_title：Letters in applied microbiology

authors： Carrasco L,Mena KD,Mota LC,Ortiz M,Behravesh CB,Gibbs SG,Bristol JR,Mayberry L,Cardenas VM

更新日期：2008-06-01 00:00:00

abstract：:Bulk tank milk from 160 producers was tested for Listeria monocytogenes at three monthly intervals over 1 year. Twenty-five producers were positive, most on a single occasion, only seven were positive on three or more of the four samplings. Listeria monocytogenes numbers were low, usually < 1 ml-1, the highest was 35 ...

journal_title：Letters in applied microbiology

authors： Fenlon DR,Stewart T,Donachie W

更新日期：1995-01-01 00:00:00

abstract：UNLABELLED:The mechanisms and organisms involved in the natural formation of volatile organohalogen compounds (VOX) are largely unknown. We provide evidence that the common and widespread soil bacterium Sinorhizobium meliloti strain 1021 is capable of producing up to 3338·6 ± 327·8 ng l(-1) headspace volume of chlorofo...

journal_title：Letters in applied microbiology

authors： Weigold P,Ruecker A,Jochmann M,Osorio Barajas XL,Lege S,Zwiener C,Kappler A,Behrens S

更新日期：2015-10-01 00:00:00

abstract：:A strain named as Pseudomonas aeruginosa 2016NX1, which could produce phenazine and cereusitin, was isolated from the root of Millettia specisoa. Phenazines were extracted, isolated and purified by chloroform, thin-layer chromatography, column chromatography and high-performance liquid chromatography. Then the purifie...

journal_title：Letters in applied microbiology

authors： Liu TT,Ye FC,Pang CP,Yong TQ,Tang WD,Xiao J,Shang CH,Lu ZJ

更新日期：2020-09-01 00:00:00

abstract：AIM:To estimate the prevalence of thermotolerant Campylobacter spp. in commercially reared partridges (Perdix perdix) in southern Italy. METHODS AND RESULTS:Cloacal swabs of partridges (n = 240), equally distributed between male and female birds, from a game bird farm located in the Southern Italy were examined for th...

journal_title：Letters in applied microbiology

authors： Dipineto L,Gargiulo A,De Luca Bossa LM,Rinaldi L,Borrelli L,Santaniello A,Menna LF,Fioretti A

更新日期：2009-09-01 00:00:00

abstract：:Nineteen strains of vancomycin-resistant Enterococcus faecium isolated from 10 of 75 (13.3%) tenderloin beef samples were examined for resistance to selected antibiotics, presence of plasmids, and genetic diversity by random amplification of polymorphic DNA analysis. All strains showed multiple resistant to the antibi...

journal_title：Letters in applied microbiology

authors： Son R,Nimita F,Rusul G,Nasreldin E,Samuel L,Nishibuchi M

更新日期：1999-08-01 00:00:00

abstract：:The ability of Stenotrophomonas maltophilia strain VUN 10,003 to degrade and detoxify high molecular weight polycyclic aromatic hydrocarbons (PAHs) was evaluated in a basal liquid medium. Using high cell density inocula of strain VUN 10,003, the concentration of pyrene, fluoranthene, benz[a]anthracene, benzo[a]pyrene,...

journal_title：Letters in applied microbiology

authors： Juhasz AL,Stanley GA,Britz ML

更新日期：2000-05-01 00:00:00

abstract：AIMS:To compare three methods for DNA extraction from Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium. METHODS AND RESULTS:The DNA was extracted from mycobacterial cultures using enzymatic extraction, combined bead beating and enzymatic extraction and cetyltrimethylammonium bromide...

journal_title：Letters in applied microbiology

authors： Amaro A,Duarte E,Amado A,Ferronha H,Botelho A

更新日期：2008-07-01 00:00:00

abstract：:The specific detection and enumeration of Lactobacillus brevis LB62, Carnobacterium divergens V14 and Carnobacterium piscicola VI were studied by in situ hybridization-flow cytometry. The method was performed on the exponential growth phase with three probes targeting 16S rRNA labelled with fluorescein isothicyanate (...

journal_title：Letters in applied microbiology

authors： Connil N,Dousset X,Onno B,Pilet MF,Breuil MF,Montel MC

更新日期：1998-11-01 00:00:00

abstract：:The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled. ...

journal_title：Letters in applied microbiology

authors： Coutinho HL,Handley BA,Kay HE,Stevenson L,Beringer JE

更新日期：1993-12-01 00:00:00

abstract：UNLABELLED:This is the first reported study on the immobilization of living recombinant Escherichia coli cells that overexpress cyclopentanone monooxygenase in polyvinyl alcohol gel particles LentiKats®. Immobilized cells overexpressing cyclopentanone monooxygenase have been used as a model of biocatalyst for enantiose...

journal_title：Letters in applied microbiology

authors： Rebroš M,Lipták L,Rosenberg M,Bučko M,Gemeiner P

更新日期：2014-06-01 00:00:00

abstract：UNLABELLED:The influence of oenological factors on cold-active pectinases from 15 preselected indigenous yeasts belonging to Aureobasidium pullulans, Filobasidium capsuligenum, Rhodotorula dairenensis, Cryptococcus saitoi and Saccharomyces cerevisiae was investigated. Pectinolytic enzymes were constitutive or partially...

journal_title：Letters in applied microbiology

authors： Merín MG,Morata de Ambrosini VI

更新日期：2015-05-01 00:00:00

abstract：AIMS:To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming. METHODS AND RESULTS:The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously con...

journal_title：Letters in applied microbiology

authors： Dong H,Tao W,Zhu L,Zhang Y,Li Y

更新日期：2011-09-01 00:00:00

abstract：AIMS:The objective of this study was to identify compounds responsible for medicinal off-flavours produced by different species and strains of Alicyclobacillus in orange juice using a combination of chromatographic-coupled olfactometric techniques and gas chromatography-mass spectrometry (GC-MS). METHODS AND RESULTS:E...

journal_title：Letters in applied microbiology

authors： Gocmen D,Elston A,Williams T,Parish M,Rouseff RL

更新日期：2005-01-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00