CAC2634-disrupted mutant of Clostridium acetobutylicum can be electrotransformed in air.

Abstract:

AIMS:To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming. METHODS AND RESULTS:The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously constructed Restriction-Modification system deficient Cl. acetobutylicum mutant SMB009 was disrupted using ClosTron method. The resulted mutant SMB012 can be electrotransformed in air with an efficiency of 1.2-3.1 × 10(3) transformants μg(-1) DNA. CONCLUSIONS:We demonstrated that the disruption of CAC2634 in Cl. acetobutylicum enables its electrotransformation in air. SIGNIFICANCE AND IMPACT OF THE STUDY:The electrotransformation process of Cl. acetobutylicum could be significantly simplified, especially when operating multiple electrotransformations.

journal_name

Lett Appl Microbiol

authors

Dong H,Tao W,Zhu L,Zhang Y,Li Y

doi

10.1111/j.1472-765X.2011.03111.x

subject

Has Abstract

pub_date

2011-09-01 00:00:00

pages

379-82

issue

3

eissn

0266-8254

issn

1472-765X

journal_volume

53

pub_type

杂志文章
  • Comparison of fermentative capacities of industrial baking and wild-type yeasts of the species Saccharomyces cerevisiae in different sugar media.

    abstract:AIMS:To compare the fermentative capacity of wild and domesticated isolates of the genus Saccharomyces. METHODS AND RESULTS:The fermentative capacity of yeasts from a variety of wild and domesticated sources was tested in synthetic dough media that mimic major bread dough types. Domesticated yeast strains were found t...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00894.x

    authors: Bell PJ,Higgins VJ,Attfield PV

    更新日期:2001-04-01 00:00:00

  • Protein A insensitive ELISA detection of staphylococcal enterotoxin B.

    abstract::A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1991.tb00601.x

    authors: Rogemond V,Da Costa Castro JM,Delaunay T,Cazaux C,Guinet RM

    更新日期:1991-09-01 00:00:00

  • Growth stimulatory effect of Ochrobactrum intermedium and Bacillus cereus on Vigna radiata plants.

    abstract:AIMS:This study assessed the plant growth-promoting ability of the bacterial strains Ochrobactrum intermedium (isolate CrT-1) and Bacillus cereus (isolate S-6). METHODS AND RESULTS:Two chromium resistant bacterial strains isolated from chromium-contaminated wastewater and soils were identified as O. intermedium CrT-1 ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01977.x

    authors: Faisal M,Hasnain S

    更新日期:2006-10-01 00:00:00

  • Biocatalysis with Escherichia coli-overexpressing cyclopentanone monooxygenase immobilized in polyvinyl alcohol gel.

    abstract:UNLABELLED:This is the first reported study on the immobilization of living recombinant Escherichia coli cells that overexpress cyclopentanone monooxygenase in polyvinyl alcohol gel particles LentiKats®. Immobilized cells overexpressing cyclopentanone monooxygenase have been used as a model of biocatalyst for enantiose...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12227

    authors: Rebroš M,Lipták L,Rosenberg M,Bučko M,Gemeiner P

    更新日期:2014-06-01 00:00:00

  • Detection of human enteric viruses in shellfish collected in Tunisia.

    abstract:AIMS:The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. METHODS AND RESULTS:Viruses were eluted from digestive tissues ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01978.x

    authors: Elamri DE,Aouni M,Parnaudeau S,Le Guyader FS

    更新日期:2006-10-01 00:00:00

  • Detection and characterization of the ferric uptake regulator (fur) gene in Plesiomonas shigelloides.

    abstract::Iron availability plays an important role in the virulence of micro-organisms, which develops different systems for iron acquisition. The expression of genes involved in iron uptake systems is usually regulated by Fur, a transcriptional regulator. Plesiomonas shigelloides is a Gram-negative food- and water-borne enter...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12858

    authors: Rodríguez-Rodríguez S,Santos JA

    更新日期:2018-04-01 00:00:00

  • Use of a putative transcriptional regulator gene as target for specific identification of Staphylococcus epidermidis.

    abstract:AIMS:To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination. METHODS AND RESULTS:Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences wer...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01948.x

    authors: Liu D,Swiatlo E,Austin FW,Lawrence ML

    更新日期:2006-09-01 00:00:00

  • A capillary polymerase chain reaction for Salmonella detection from poultry meat.

    abstract:AIMS:In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. METHODS AND RESULTS:Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02033.x

    authors: Gunaydin E,Eyigor A,Carli KT

    更新日期:2007-01-01 00:00:00

  • A preliminary assessment of Bacillus anthracis spore inactivation using an electrochemically activated solution (ECASOL).

    abstract:AIM:To evaluate the efficacy of electrochemically activated solution (ECASOL) in decontaminating Bacillus anthracis Ames and Vollum 1B spores, with and without changing the source water hardness and final ECASOL pH. METHODS AND RESULTS:Five different ECASOL formulations were generated, in which the source water hardne...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02002.x

    authors: Rogers JV,Ducatte GR,Choi YW,Early PC

    更新日期:2006-11-01 00:00:00

  • Citric acid production by Aspergillus niger on wet corn distillers grains.

    abstract:AIMS:To determine which citric acid-producing strain of Aspergillus niger utilized wet corn distillers grains most effectively to produce citric acid. METHODS AND RESULTS:Citric acid and biomass production by the fungal strains were analysed on the untreated grains or autoclaved grains using an enzyme assay and a grav...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01958.x

    authors: Xie G,West TP

    更新日期:2006-09-01 00:00:00

  • Genetic heterogeneity of non-O1 and non-O139 Vibrio cholerae isolates from shrimp aquaculture system: a comparison of RS-, REP- and ERIC-PCR fingerprinting approaches.

    abstract:AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02863.x

    authors: Rao BM,Surendran PK

    更新日期:2010-07-01 00:00:00

  • Cold storage affects survival and growth of Erwinia amylovora on the calyx of apple.

    abstract:AIMS:To determine the effect of cold storage on the survival of Erwinia amylovora. METHODS AND RESULTS:The survival of E. amylovora was assessed during storage at 2 degrees C. Populations of E. amylovora inoculated into phosphate-buffered saline remained static, whereas in nutrient media populations increased at low t...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01407.x

    authors: Taylor RK,Hale CN

    更新日期:2003-01-01 00:00:00

  • Effect of hydrostatic pressure on the morphology and ultrastructure of wild-type and trehalose synthase mutant cells of Saccharomyces cerevisiae.

    abstract:AIMS:Saccharomyces cerevisiae was used for studying the physiological effects of hydrostatic pressure. METHODS AND RESULTS:The effects of hydrostatic pressure on the ultrastructure of wild-type and trehalose-6-phosphate synthase (tps1) mutant cells were investigated by transmission electron microscopy. Pressure induce...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00853.x

    authors: Fernandes PM,Farina M,Kurtenbach E

    更新日期:2001-01-01 00:00:00

  • Environmental factors influencing the inactivation of Listeria monocytogenes by pulsed electric fields.

    abstract:AIMS:To investigate the influence of the growth phase, growth temperature, storage time, pH and aw of the treatment medium on the resistance of Listeria monocytogenes to pulsed electric fields (PEF). METHODS AND RESULTS:Square wave pulses of 2 micros at a frequency of 1 Hz and 25 and 28 kV cm(-1) were used. Cells were...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01221.x

    authors: Alvarez I,Pagán R,Raso J,Condón S

    更新日期:2002-01-01 00:00:00

  • Antibacterial activity of bovine lactoferrin and its peptides against enterohaemorrhagic Escherichia coli O157:H7.

    abstract::The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 m...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00358.x

    authors: Shin K,Yamauchi K,Teraguchi S,Hayasawa H,Tomita M,Otsuka Y,Yamazaki S

    更新日期:1998-06-01 00:00:00

  • Studies of the release of small molecules during pressure germination of spores of Bacillus subtilis.

    abstract:AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spore...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02204.x

    authors: Vepachedu VR,Hirneisen K,Hoover DG,Setlow P

    更新日期:2007-09-01 00:00:00

  • Differential activity profiles of translation inhibitors in whole-cell and cell-free approaches.

    abstract:AIMS:Evaluation of the activity profiles of standard prokaryotic translation inhibitors with different physicochemical properties under whole-cell and cell-free conditions. METHODS AND RESULTS:The minimal inhibitory concentration values (cell-free/whole-cell microg ml(-1)) for three aminoglycosides (neomycin, 0.01/6.9...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02281.x

    authors: Weidlich M,Klammt C,Bernhard F,Karas M,Stein T

    更新日期:2008-02-01 00:00:00

  • Survival and persistence of nonspore-forming biothreat agents in water.

    abstract:AIMS:To determine whether nonspore-forming biothreat agents can survive and persist in potable water that does not contain a disinfectant. METHODS AND RESULTS: Autoclaved, de-chlorinated Atlanta municipal water was inoculated with eight isolates of bacterial biothreat agents (10⁶ CFU ml⁻¹). The inoculated water sample...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2012.03277.x

    authors: Gilbert SE,Rose LJ

    更新日期:2012-09-01 00:00:00

  • Quantitative RT-PCR for the enumeration of noroviruses (Norwalk-like viruses) in water and sewage.

    abstract:AIMS:Aims of investigation: (i) develop a quantitative RT-PCR for noroviruses and (ii) evaluate it on environmental samples. METHODS AND RESULTS:Noroviruses in environmental water samples were concentrated by adsorption/elution/flocculation. Sewage was processed by clarification and protein flocculation. Norovirus-spe...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2004.01534.x

    authors: Laverick MA,Wyn-Jones AP,Carter MJ

    更新日期:2004-01-01 00:00:00

  • Characterization and comparison of microbial community of different typical Chinese liquor Daqus by PCR-DGGE.

    abstract:AIMS:To identify and compare microbiota in Chinese liquor Daqu, which were produced in the different regions using different production process. METHODS AND RESULTS:The DNA exacted from Daqu samples was used as a template for PCR with universal primers of 16S rRNA, 26S rRNA and 18S rRNA, respectively. The amplicons we...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03076.x

    authors: Wang HY,Gao YB,Fan QW,Xu Y

    更新日期:2011-08-01 00:00:00

  • Evidence of the production of silver nanoparticles via pretreatment of Phoma sp.3.2883 with silver nitrate.

    abstract:AIMS:The objective was to demonstrate the size of silver particles produced by the filamentous fungus Phoma sp.3.2883 via adsorption and accumulation, and to confirm that this silver was in a reduced state. METHODS AND RESULTS:Mycelium was freeze-dried and then shake-cultured in a silver nitrate solution. It was found...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01348.x

    authors: Chen JC,Lin ZH,Ma XX

    更新日期:2003-01-01 00:00:00

  • Modeling the inactivation kinetics of bacillus spores by glutaraldehyde.

    abstract:AIMS:Our goal was to develop a mathematical kinetic model to predict the sporicidal activity of glutaraldehyde, which is an active ingredient frequently used in commercial products employed for liquid disinfection and decontamination. METHODS AND RESULTS:We used our previously published data on spore inactivation by g...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02358.x

    authors: Retta SM,Sagripanti JL

    更新日期:2008-05-01 00:00:00

  • Inactivation of Salmonella enterica serovar enteritidis in shell eggs by sequential application of heat and ozone.

    abstract:AIMS:To assess the contribution of ozone to lethality of Salmonella enterica serovar Enteritidis in experimentally inoculated whole shell eggs that are sequentially treated with heat and gaseous ozone in pilot-scale equipment. METHODS AND RESULTS:Whole shell eggs were inoculated with small populations of Salmonella En...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02367.x

    authors: Perry JJ,Rodriguez-Romo LA,Yousef AE

    更新日期:2008-06-01 00:00:00

  • Potential chitinase activating factor from yeast cells of Candida albicans.

    abstract::Microsomal chitinase from yeast and hyphal cells of Candida albicans was activated endogenously by incubation at 30 degrees C and exogenously by trypsin. The putative activating factor of yeast cells was separated from chitinase activity by fractionation of lysed protoplasts on an Iodixanol density gradient. The vacuo...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb00054.x

    authors: Jackson DJ,Saunders VA,Humphreys AM

    更新日期:1996-09-01 00:00:00

  • Faecal fatty acids and gastrointestinal upset in newborn infants.

    abstract::Patterns of long-chain faecal fatty acids were studied by gas-liquid chromatography in 55 newborn infants in a neonatal intensive care unit. Decreased fractions of fatty acid C16:1 and increased fractions of C16:0 and C17:0 were associated with the occurrence of abdominal distension. Decreased fractions of C16:1 and C...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00341.x

    authors: Ahtonen P,Lehtonen OP,Kero P,Eerola E

    更新日期:1997-02-01 00:00:00

  • Selective growth of Staphylococcus aureus from flushed dairy manure wastewater using acriflavine-supplemented mannitol salt agar.

    abstract:AIMS:To investigate the use of mannitol salt agar (MSA) supplemented with acriflavine for selective growth and quantification of Staphylococcus aureus from flushed dairy manure wastewater (FDMW). METHODS AND RESULTS:Minimal inhibitory concentrations of acriflavine in MSA were determined by comparing the growth of S. a...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01915.x

    authors: Davis JA,Farrah SR,Wilkie AC

    更新日期:2006-06-01 00:00:00

  • Degradation of quillaja saponins by mixed culture of rumen microbes.

    abstract::Quillaja saponin (QS) was incubated at 39 degrees C in an in vitro medium containing rumen liquor from a cow fed a roughage diet. No degradation of QS was observed up to 6 h of fermentation. Incubation for 9, 12 and 24 h decreased the content of QS by 16%, 45% and 100%. The content of QS did not decrease when incubate...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00207.x

    authors: Makkar HP,Becker K

    更新日期:1997-10-01 00:00:00

  • Adaptation of Listeria monocytogenes in a simulated cheese medium: effects on virulence using the Galleria mellonella infection model.

    abstract:UNLABELLED:The aim of this study was to evaluate the effect of the acid and salt adaptation in a cheese-based medium on the virulence potential of Listeria monocytogenes strains isolated from cheese and dairy processing environment using the Galleria mellonella model. Four L. monocytogenes strains were exposed to a che...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12064

    authors: Schrama D,Helliwell N,Neto L,Faleiro ML

    更新日期:2013-06-01 00:00:00

  • Influence of interactions between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in a defined medium.

    abstract:AIMS:To investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in modified Pine's medium (Pine et al. 1989). METHODS AND RESULTS:Modified Pine's medium containing 0, 0.044, 0.088 or 0.176 g l(-1) ferric ammonium citrate, and 0 or 1 mM ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01237.x

    authors: Dykes GA,Dworaczek M

    更新日期:2002-01-01 00:00:00

  • Identification and characterization of a new three-component nicotinic acid hydroxylase NahAB1 B2 from Pusillimonas sp. strain T2.

    abstract::Nicotinic acid (NA) is ubiquitous in nature and its microbial degradation mechanisms are diverse. In this study, Pusillimonas sp. strain T2 was found to be capable of utilizing NA as sole carbon and nitrogen sources. This strain could completely degrade 300 mg l-1 NA within 3·5 h at 30°C and pH 7·0 and one of the degr...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12850

    authors: Yuan M,Zhang Y,Zhao L,Ma Y,He Q,He J,Qiu J

    更新日期:2018-04-01 00:00:00