Detection of human enteric viruses in shellfish collected in Tunisia.

Abstract:

AIMS:The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. METHODS AND RESULTS:Viruses were eluted from digestive tissues and concentrated by polyethylene glycol precipitation before nucleic acid extraction and purification. After checking for inhibitors, all viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by hybridization. Overall, 83% of the samples were found positive for at least one virus. Astrovirus was detected in 61% of the samples, norovirus in 35% and HAV in 26%. Surprisingly, only one sample was found positive for enterovirus. CONCLUSIONS:The mean number of positive samples found in this study is in accordance with the data found in the literature, indicating that no real difference exists in this respect among countries studied. A notable exception is HAV, which reflects the epidemiological status of the population. SIGNIFICANCE AND IMPACT OF THE STUDY:This study highlights the interest to analyse shellfish samples from different production areas. These data will be helpful to understand virus circulation and to improve shellfish safety. The results, which confirm contamination, necessitate the development of appropriate studies and monitoring in all shellfish-producing countries.

journal_name

Lett Appl Microbiol

authors

Elamri DE,Aouni M,Parnaudeau S,Le Guyader FS

doi

10.1111/j.1472-765X.2006.01978.x

subject

Has Abstract

pub_date

2006-10-01 00:00:00

pages

399-404

issue

4

eissn

0266-8254

issn

1472-765X

pii

LAM1978

journal_volume

43

pub_type

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