Isolation and characterization of multidrug-resistant Leclercia species from animal clinical case.

abstract：AIMS:To develop and evaluate a new and reliable real-time PCR detection protocol on chromosomal DNA of the contagious plant pathogenic bacterium Erwinia amylovora, the causal agent of fire blight. METHODS AND RESULTS:A Taqman minor-groove-binder real-time PCR assay targeting a hypothetical protein coding gene of Erw. ...

journal_title：Letters in applied microbiology

authors： Gottsberger RA

更新日期：2010-09-01 00:00:00

abstract：AIMS:To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS:Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x1...

journal_title：Letters in applied microbiology

authors： La Ragione RM,Narbad A,Gasson MJ,Woodward MJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed. METHODS AND RESULTS:Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in ...

journal_title：Letters in applied microbiology

authors： McEwan NR,Abecia L,Regensbogenova M,Adam CL,Findlay PA,Newbold CJ

更新日期：2005-01-01 00:00:00

abstract：:Live shellfish samples (120) were collected from nine littoral sites in Brittany (western France). They were screened for Listeria spp. and a count of faecal coliforms was carried out. Analysis of the results revealed Listeria spp. in 55% of samples, a much higher rate than the previous, infrequent, recorded data. Fur...

journal_title：Letters in applied microbiology

authors： Monfort P,Minet J,Rocourt J,Piclet G,Cormier M

更新日期：1998-03-01 00:00:00

abstract：:Samples were collected from 148 adult cats, processed for isolation of Staphylococcus species and tested for susceptibility to penicillin G, gentamicin, oxacillin, amoxycillin, ampicillin, cephalexin and rifampin. Methicillin resistance was also determined. Ninety-eight isolates were obtained (66% of samples). Coagula...

journal_title：Letters in applied microbiology

authors： Lilenbaum W,Nunes EL,Azeredo MA

更新日期：1998-10-01 00:00:00

abstract：AIMS:This work aims to investigate the binding capability of certain domains at N terminus of the protein NP_785232 of Lactobacillus plantarum to Caco-2 cells and to test the usage of affinity chromatography to isolate the human mucus component that interacts with them. METHODS AND RESULTS:Recombinant proteins contain...

journal_title：Letters in applied microbiology

authors： Du L,Liu F,Ju X,Huo G

更新日期：2010-10-01 00:00:00

abstract：AIMS:To investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in modified Pine's medium (Pine et al. 1989). METHODS AND RESULTS:Modified Pine's medium containing 0, 0.044, 0.088 or 0.176 g l(-1) ferric ammonium citrate, and 0 or 1 mM ...

journal_title：Letters in applied microbiology

authors： Dykes GA,Dworaczek M

更新日期：2002-01-01 00:00:00

abstract：AIMS:To compare a range of enrichment broths and enrichment temperatures for the isolation of Escherichia coli O157 by immunomagnetic separation (IMS) from sandy, loam and clay soils. METHODS AND RESULTS:Soils were spiked with cocktails of four atoxigenic strains of E. coli O157 and four strains of commensal E. coli. ...

journal_title：Letters in applied microbiology

authors： Hepburn NF,MacRae M,Johnston M,Mooney J,Ogden ID

更新日期：2002-01-01 00:00:00

abstract：AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in dochi (fermented black beans), a traditional fermented food in Taiwan. METHODS AND RESULTS:A total of 30 samples were collected from three different dochi producers and analysed after different periods of storage. Fifty-two cultures of LAB were i...

journal_title：Letters in applied microbiology

authors： Chen YS,Yanagida F,Hsu JS

更新日期：2006-08-01 00:00:00

abstract：AIMS:To develop a novel method for rapid construction of Campylobacter jejuni deletion mutants. METHODS AND RESULTS:We used overlapping extension PCR protocol to amplify a target sequence region of Camp. jejuni genomic DNA in which an internal fragment, Cj0618 coding sequence, was replaced by a chloramphenicol resista...

journal_title：Letters in applied microbiology

authors： Hansen CR,Khatiwara A,Ziprin R,Kwon YM

更新日期：2007-12-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are polyesters accumulated by many bacteria under unbalanced growth conditions, and have been used to meet the various demands in areas of agriculture, medicine, and materials especially belong to a rapidly expanding area of biomedical research. Unfortunately, the high production cost than...

journal_title：Letters in applied microbiology

authors： Liu CC,Zhang LL,An J,Chen B,Yang H

更新日期：2016-01-01 00:00:00

abstract：:Nisin, a bacteriocin produced by some strains of Lactococcus lactis, acts against foodborne pathogen Listeria monocytogenes. A single exposure of cells to nisin can generate nisin-resistant (Nisr) mutants, which may compromise the use of nisin in the food industry. The objective of this research was to compare the hea...

journal_title：Letters in applied microbiology

authors： Modi KD,Chikindas ML,Montville TJ

更新日期：2000-03-01 00:00:00

abstract：:Patterns of long-chain faecal fatty acids were studied by gas-liquid chromatography in 55 newborn infants in a neonatal intensive care unit. Decreased fractions of fatty acid C16:1 and increased fractions of C16:0 and C17:0 were associated with the occurrence of abdominal distension. Decreased fractions of C16:1 and C...

journal_title：Letters in applied microbiology

authors： Ahtonen P,Lehtonen OP,Kero P,Eerola E

更新日期：1997-02-01 00:00:00

abstract：:Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram-positive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which re...

journal_title：Letters in applied microbiology

authors： McCormick JK,Klaenhammer TR,Stiles ME

更新日期：1999-07-01 00:00:00

abstract：AIMS:Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at in...

journal_title：Letters in applied microbiology

authors： Westers H,Darmon E,Zanen G,Veening JW,Kuipers OP,Bron S,Quax WJ,van Dijl JM

更新日期：2004-01-01 00:00:00

abstract：AIM:To evaluate the effect of starvation, heat, cold, acid, alkaline, chlorine and ethanol stresses on the resistance of Enterobacter sakazakii in powdered infant milk formula (PIMF) towards gamma radiation. METHODS AND RESULTS:Stressed cells of E. sakazakii ATCC 51329 and four other food isolate strains were mixed in...

journal_title：Letters in applied microbiology

authors： Osaili T,Al-Nabulsi A,Shaker R,Ayyash M,Olaimat A,Abu Al-Hasan A,Kadora K,Holley R

更新日期：2008-08-01 00:00:00

abstract：:Microsomal chitinase from yeast and hyphal cells of Candida albicans was activated endogenously by incubation at 30 degrees C and exogenously by trypsin. The putative activating factor of yeast cells was separated from chitinase activity by fractionation of lysed protoplasts on an Iodixanol density gradient. The vacuo...

journal_title：Letters in applied microbiology

authors： Jackson DJ,Saunders VA,Humphreys AM

更新日期：1996-09-01 00:00:00

abstract：AIMS:The aim of this study was to investigate the role of divercin V41 in inhibition and prevention of Listeria monocytogenes. METHODS AND RESULTS:Carnobacterium divergens V41 deficient in bacteriocin production was isolated and characterized by enzyme-liked immunosorbent assay, multiplex polymerase chain reaction and...

journal_title：Letters in applied microbiology

authors： Richard C,Brillet A,Pilet MF,Prévost H,Drider D

更新日期：2003-01-01 00:00:00

abstract：AIMS:To immobilize Methylobacterium sp. NP3 and Acinetobacter sp. PK1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol. METHODS AND RESULTS:The phenol degradation activity of suspended and immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 bacteria wa...

journal_title：Letters in applied microbiology

authors： Khongkhaem P,Intasiri A,Luepromchai E

更新日期：2011-05-01 00:00:00

abstract：:The Crystal Enteric/Nonfermenter (E/NF) identification kit (Becton Dickinson Microbiology Systems, USA) was evaluated using water-derived bacterial isolates and results compared to those obtained by the API 20E system (BioMérieux, UK). Both the E/NF and 20E systems correctly identified 93% of the Enterobacteriaceae re...

journal_title：Letters in applied microbiology

authors： Micklewright IJ,Sartory DP

更新日期：1995-09-01 00:00:00

abstract：AIMS:To examine the ability of Agrobacterium to attach to Metarhizium anisopliae var. acridum strain CG423 under co-cultivation and to develop an Agrobacterium-mediated method of gene delivery into strain CG423, a promising agent for biological control of grasshoppers. METHODS AND RESULTS:The co-cultivation of Agrobac...

journal_title：Letters in applied microbiology

authors： Duarte RT,Staats CC,Fungaro MH,Schrank A,Vainsten MH,Furlaneto-Maia L,Nakamura CV,de Souza W,Furlaneto MC

更新日期：2007-03-01 00:00:00

abstract：AIMS:To compare three methods for DNA extraction from Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium. METHODS AND RESULTS:The DNA was extracted from mycobacterial cultures using enzymatic extraction, combined bead beating and enzymatic extraction and cetyltrimethylammonium bromide...

journal_title：Letters in applied microbiology

authors： Amaro A,Duarte E,Amado A,Ferronha H,Botelho A

更新日期：2008-07-01 00:00:00

abstract：AIMS:To isolate gamma-hexachlorocyclohexane (gamma-HCH)-degrading bacteria from a single field and to examine their genetic diversity. METHODS AND RESULTS:Gamma-HCH-degrading bacteria were screened from a long-term experimental field in which gamma-HCH has been continuously applied to, and a gamma-HCH-degrading sphing...

journal_title：Letters in applied microbiology

authors： Yamamoto S,Otsuka S,Murakami Y,Nishiyama M,Senoo K

更新日期：2009-10-01 00:00:00

abstract：:Control of biofouling on seawater reverse osmosis (SWRO) membranes is a major challenge as treatments can be expensive, damage the membrane material and often biocides do not remove the polymers in which bacteria are embedded. Biological control has been largely ignored for biofouling control. The objective of this st...

journal_title：Letters in applied microbiology

authors： Nagaraj V,Skillman L,Li D,Xie Z,Ho G

更新日期：2017-07-01 00:00:00

abstract：AIMS:  The primary goal of this study was to characterize the existence of a functional c-di-GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. METHODS AND RESULTS:  A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the ...

journal_title：Letters in applied microbiology

authors： Ruiz LM,Castro M,Barriga A,Jerez CA,Guiliani N

更新日期：2012-02-01 00:00:00

abstract：:PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni a...

journal_title：Letters in applied microbiology

authors： Rasmussen HN,Olsen JE,Jørgensen K,Rasmussen OF

更新日期：1996-11-01 00:00:00

abstract：AIMS:Acetic acid is considered an important preservative in tropical grass ensiling. The objective of the current experiments was to follow the ensiling fermentation of low dry matter (DM) tropical grass as a model to study changes in bacterial communities during acetic acid fermentation. METHODS AND RESULTS:Direct-cu...

journal_title：Letters in applied microbiology

authors： Nishino N,Li Y,Wang C,Parvin S

更新日期：2012-03-01 00:00:00

abstract：AIMS:A rapid and simple method for enumerating uninjured and sublethally injured bacterial cells, the twofold dilution method (2FD), was developed and evaluated. METHODS AND RESULTS:Following twofold serial dilution of samples in a 96 well microtiter plate, double strength selective broth or nonselective broth was add...

journal_title：Letters in applied microbiology

authors： Kang DH,Siragusa GR

更新日期：2001-09-01 00:00:00

abstract：:Yellow wine lees (YWL) are the main co-products in yellow wine industry with unbalanced amino acid (AA) profiles. Solid-state fermentation was employed in this study to upgrade the YWL for ruminant animals. A 3 × 3 orthogonal design was conducted to optimize the fermentation condition for optimal crude protein (CP) yi...

journal_title：Letters in applied microbiology

authors： Yao KY,Zhang TZ,Wang HF,Liu JX

更新日期：2018-12-01 00:00:00

abstract：:The aim of this study was to determine the epidemiology (location, microbial load, microbiome, presence/absence of biofilm and pathogens, including ESKAPE-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, and antimicrobial susc...

journal_title：Letters in applied microbiology

authors： Costa DM,Johani K,Melo DS,Lopes LKO,Lopes Lima LKO,Tipple AFV,Hu H,Vickery K

更新日期：2019-04-01 00:00:00