Abstract:
AIMS:Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at investigating a possible role of TepA in secretion stress management, and characterizing the intensity of the secretion stress response in relation to alpha-amylase production. METHODS AND RESULTS:The expression of a transcriptional htrB-lacZ gene fusion, and the levels of alpha-amylase production were monitored simultaneously using tepA mutant B. subtilis strains. TepA was shown to be dispensable for secretion stress management. Importantly, however, the levels of htrB-lacZ expression can be correlated with the levels of alpha-amylase production. CONCLUSION:Our observations show that the secretion stress response can serve as an indicator for alpha-amylase production levels. SIGNIFICANCE AND IMPACT OF STUDY:Conceivably, this stress response can be employed to monitor the biotechnological production of various secretory proteins by the Bacillus cell factory.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Westers H,Darmon E,Zanen G,Veening JW,Kuipers OP,Bron S,Quax WJ,van Dijl JMdoi
10.1111/j.1472-765X.2004.01539.xsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
65-73issue
1eissn
0266-8254issn
1472-765Xpii
LAM1539journal_volume
39pub_type
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