Specific PCR-based detection of Phomopsis heveicola the cause of leaf blight of Coffee (Coffea arabica L.) in China.

abstract：:Salmonella enteritidis-inoculated poultry sausages were pressurized at 500 MPa by combining different times (10 and 30 min) and temperatures (50, 60 and 70 degrees C) or heat treated with the same temperature-time combinations and a standard cooking (75 degrees C for 30 min). Counts of Salm. enteritidis and mesophilic...

journal_title：Letters in applied microbiology

authors： Yuste J,Pla R,Mor-Mur M

更新日期：2000-11-01 00:00:00

abstract：AIMS:The antifungal activity of amyrin pentacyclic triterpene and 15 synthetic derivatives was evaluated against Candida species. Additionally, inhibition of adhesion of Candida albicans to human epithelial cells in vitro was determined. METHODS AND RESULTS:Esterification of alpha- and beta-amyrin with a variety of ac...

journal_title：Letters in applied microbiology

authors： Johann S,Soldi C,Lyon JP,Pizzolatti MG,Resende MA

更新日期：2007-08-01 00:00:00

abstract：AIMS: To develop a new instrument of great versatility for recovering micro-organisms from all types of food samples and to compare the effects with existing sample preparation methods. METHODS AND RESULTS: To detach micro-organisms from large-size unbroken food samples such as apples, carrots, potatoes and tomatoes w...

journal_title：Letters in applied microbiology

authors： Kim YH,Lee SY,Sagong HG,Heu S,Ryu S,Kang DH

更新日期：2012-09-01 00:00:00

abstract：AIMS:To identify and compare microbiota in Chinese liquor Daqu, which were produced in the different regions using different production process. METHODS AND RESULTS:The DNA exacted from Daqu samples was used as a template for PCR with universal primers of 16S rRNA, 26S rRNA and 18S rRNA, respectively. The amplicons we...

journal_title：Letters in applied microbiology

authors： Wang HY,Gao YB,Fan QW,Xu Y

更新日期：2011-08-01 00:00:00

abstract：:Reagent-derived contamination can compromise the integrity of microbiome data, particularly in low microbial biomass samples. This contamination has recently been attributed to the 'kitome' (contamination introduced by the DNA extraction kit), prior to which attention was mostly paid to potential contamination introdu...

journal_title：Letters in applied microbiology

authors： Stinson LF,Keelan JA,Payne MS

更新日期：2019-01-01 00:00:00

abstract：:Candida-associated denture stomatitis presents as erythema of the palatal mucosa and is caused by biofilms containing the fungus Candida albicans that co-reside with oral bacteria on the denture-fitting surface. This study aimed to assess the effect of several frequently encountered oral bacteria on the expression of ...

journal_title：Letters in applied microbiology

authors： Morse DJ,Wilson MJ,Wei X,Bradshaw DJ,Lewis MAO,Williams DW

更新日期：2019-04-01 00:00:00

abstract：UNLABELLED:Lactobacillus acidophilus LAB20 has potential to be a probiotic strain because it can be present at high numbers in the jejunum of dog. To specifically detect LAB20 from dog faecal samples, a real-time PCR protocol was developed targeting the novel surface (S) layer protein gene of LAB20. The presence of S-l...

journal_title：Letters in applied microbiology

authors： Tang Y,Saris PE

更新日期：2013-10-01 00:00:00

abstract：UNLABELLED:Most plant species are colonized by endophytic bacteria. Despite their importance for plant health and growth, the response of these bacteria to grassland management regimes is still not understood. Hence, we investigated the bacterial community structure in three agricultural important grass species Dactyli...

journal_title：Letters in applied microbiology

authors： Wemheuer F,Wemheuer B,Kretzschmar D,Pfeiffer B,Herzog S,Daniel R,Vidal S

更新日期：2016-04-01 00:00:00

abstract：:A Multiplex PCR-based assay (m-PCR) with three sets of primers was developed for the detection of all serotypes of Salmonella enterica and the identification of Salmonella Enteritidis and Salmonella Typhimurium. This method was evaluated against a bacteriological method for the analysis of environmental swabs of poult...

journal_title：Letters in applied microbiology

authors： Soumet C,Ermel G,Rose N,Rose V,Drouin P,Salvat G,Colin P

更新日期：1999-02-01 00:00:00

abstract：AIMS:To investigate fusion expression between Bacillus thuringiensis crystal protein and a foreign protein, the expression of a fusion protein comprised of Cry1Ac, and enhanced green fluorescent protein (EGFP) in B. thuringiensis Cry(-)B strain was examined. METHODS AND RESULTS:The N-terminal fusion expression of EGFP...

journal_title：Letters in applied microbiology

authors： Roh JY,Li MS,Chang JH,Choi JY,Shim HJ,Shin SC,Boo KS,Je YH

更新日期：2004-01-01 00:00:00

abstract：:Pyrimidine synthesis in the food spoilage agent Burkholderia cepacia ATCC 25416 was investigated. The five de novo pathway enzymes of pyrimidine biosynthesis were found to be active in B. cepacia ATCC 25416 and growth of this strain on uracil had an effect on the de novo enzyme activities. The in vitro regulation of a...

journal_title：Letters in applied microbiology

authors： Li K,West TP

更新日期：1995-11-01 00:00:00

abstract：UNLABELLED:Chrysanthemum plants are subject to serious virus diseases, so detection and identification of virus pathogens is important to prevent the virus spread. A reliable one-step multiplex RT-PCR was developed to simultaneously detect two viruses and two viriods: chrysanthemum virus B, tomato Aspermy virus, chrysa...

journal_title：Letters in applied microbiology

authors： Song A,You Y,Chen F,Li P,Jiang J,Chen S

更新日期：2013-01-01 00:00:00

abstract：AIMS:In the present study, two different optimization techniques were used to determine the suitable operating parameters for exo-biopolymer production in submerged mycelial cultures of two entomopathogenic fungi Paecilomyces japonica and Paecilomyces tenuipes. METHODS AND RESULTS:First, the rotating simplex method, a...

journal_title：Letters in applied microbiology

authors： Xu CP,Sinha J,Bae JT,Kim SW,Yun JW

更新日期：2006-05-01 00:00:00

abstract：:A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for separating homologous amplicons from the respective uidA region differing in one to seven single base substitutions. Bo...

journal_title：Letters in applied microbiology

authors： Farnleitner AH,Kreuzinger N,Kavka GG,Grillenberger S,Rath J,Mach RL

更新日期：2000-06-01 00:00:00

abstract：:In order to select a suitable indicator for monitoring the pathogen regrowth potential of composted biosolids, the growth kinetics of selected bacteria were investigated. Growth parameters of six serovars of Salmonella and three strains of Escherichia coli in sterilized compost were compared. Seeded Salmonella and E. ...

journal_title：Letters in applied microbiology

authors： Sidhu J,Gibbs RA,Ho GE,Unkovich I

更新日期：1999-11-01 00:00:00

abstract：AIMS:To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS). METHODS AND RESULTS:CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time interv...

journal_title：Letters in applied microbiology

authors： Pellé E,Dousset X,Prévost H,Drider D

更新日期：2005-01-01 00:00:00

abstract：:In a crossover study, volunteers consumed a diet supplemented with either wheat or rye bread. Levels of faecal mucus were determined and the adhesion of five selected probiotics to immobilized intestinal mucus, was assessed. No significant difference in the concentration of faecal mucus or adhesion of the probiotics t...

journal_title：Letters in applied microbiology

authors： Ouwehand AC,Gråsten S,Niemi P,Mykkänen H,Salminen S

更新日期：2000-07-01 00:00:00

abstract：AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...

journal_title：Letters in applied microbiology

authors： Rao BM,Surendran PK

更新日期：2010-07-01 00:00:00

abstract：AIM:To compare the incidence of antimicrobial resistance among Salmonella serotypes isolated in a pig slaughterhouse in Zaragoza (Spain) during 1993 and 2001. METHODS AND RESULTS:A total of 168 isolates representing 10 serotypes were examined by disc diffusion method using 17 antibiotics. Data showed that the majority...

journal_title：Letters in applied microbiology

authors： Agustín AI,Carramiñana JJ,Rota C,Herrera A

更新日期：2005-01-01 00:00:00

abstract：AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spore...

journal_title：Letters in applied microbiology

authors： Vepachedu VR,Hirneisen K,Hoover DG,Setlow P

更新日期：2007-09-01 00:00:00

abstract：AIMS:Aim of the study was to develop a medium for optimal heparinase production with a strain of Aspergillus flavus (MTCC-8654) by using a multidimensional statistical approach. METHODS AND RESULTS:Statistical optimization of intracellular heparinase production by A. flavus, a new isolate, was investigated. Plackett-B...

journal_title：Letters in applied microbiology

authors： Banga J,Tripathi CK

更新日期：2009-08-01 00:00:00

abstract：AIMS:To evaluate the effect of protective agents upon survival of Lactobacillus delbrueckii ssp. bulgaricus during freeze-drying and storage, and selective amino acids on cell membrane fluidity. METHODS AND RESULTS:The protective effect of amino-acids and sugars at different concentrations was studied by determining t...

journal_title：Letters in applied microbiology

authors： Martos GI,Minahk CJ,de Valdez GF,Morero R

更新日期：2007-09-01 00:00:00

abstract：AIMS:Genista saharae, indigenous of Sahara, is a spontaneous shrub that plays an important ecological role for the preservation and fertility of poor and eroded soils. This legume has not been examined for its root nodule bacteria. The taxonomic diversity of bacteria from root nodules of G. saharae growing in the infra...

journal_title：Letters in applied microbiology

authors： Mahdhi M,Nzoué A,Gueye F,Merabet C,de Lajudie P,Mars M

更新日期：2007-12-01 00:00:00

abstract：AIMS:To evaluate the effect of oregano essential oil on Listeria monocytogenes cytoplasmic membrane. METHODS AND RESULTS:Nitroxide free-radical Electron Paramagnetic Resonance was applied on L. monocytogenes after 30 min exposure to oregano essential oil concentrations ranging from 0 to 1.25%. The impact of essential ...

journal_title：Letters in applied microbiology

authors： Serio A,Chiarini M,Tettamanti E,Paparella A

更新日期：2010-08-01 00:00:00

abstract：:A 21-mer DNA oligonucleotide probe targeting the 23S rRNA of Mycobacterium leprae was developed and its high specificity demonstrated by dot-blot hybridization. Even under relaxed hybridization and washing conditions (20 degrees C below Tm) the probe was highly selective in that positive signals were only detected wit...

journal_title：Letters in applied microbiology

authors： Liesack W,Pitulle C,Stackebrandt E

更新日期：1990-08-01 00:00:00

abstract：AIM:To determine if the outer membrane (OM) cytochromes OmcA and OmcB of the metal-reducing bacterium Shewanella oneidensis MR-1 have distinct or overlapping roles in the reduction of insoluble manganese(IV) oxide. METHODS AND RESULTS:The gene replacement mutant (OMCA1) which lacks OmcA was partially deficient in Mn(I...

journal_title：Letters in applied microbiology

authors： Myers JM,Myers CR

更新日期：2003-01-01 00:00:00

abstract：UNLABELLED:The influence of oenological factors on cold-active pectinases from 15 preselected indigenous yeasts belonging to Aureobasidium pullulans, Filobasidium capsuligenum, Rhodotorula dairenensis, Cryptococcus saitoi and Saccharomyces cerevisiae was investigated. Pectinolytic enzymes were constitutive or partially...

journal_title：Letters in applied microbiology

authors： Merín MG,Morata de Ambrosini VI

更新日期：2015-05-01 00:00:00

abstract：AIMS:Aims of investigation: (i) develop a quantitative RT-PCR for noroviruses and (ii) evaluate it on environmental samples. METHODS AND RESULTS:Noroviruses in environmental water samples were concentrated by adsorption/elution/flocculation. Sewage was processed by clarification and protein flocculation. Norovirus-spe...

journal_title：Letters in applied microbiology

authors： Laverick MA,Wyn-Jones AP,Carter MJ

更新日期：2004-01-01 00:00:00

abstract：:Colistin-resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high-resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known geno...

journal_title：Letters in applied microbiology

authors： Tahmasebi H,Dehbashi S,Arabestani MR

更新日期：2020-04-01 00:00:00

abstract：UNLABELLED:Palm wine produced traditionally and consumed by many people in the South-West of Burkina Faso is subject to alteration. In this study, we carried out a follow-up of two palm wines' fermentation during the 10 days in which palm wines are classically produced and consumed. We monitored biochemical characteris...

journal_title：Letters in applied microbiology

authors： Tapsoba F,Savadogo A,Legras JL,Zongo C,Traore AS

更新日期：2016-10-01 00:00:00