Rapid construction of Campylobacter jejuni deletion mutants.

abstract：AIMS:Rapid detection of pathogenic Yersinia enterocolitica isolates by using antisera raised against recombinant attachment-invasion locus (Ail) protein. METHODS AND RESULTS:The complete gene (471 bp) encoding for the Ail protein was amplified by PCR and cloned in pQE 30 UA vector. The recombinant clones were selected...

journal_title：Letters in applied microbiology

authors： Balakrishna K,Murali HS,Batra HV

更新日期：2010-02-01 00:00:00

abstract：UNLABELLED:Escherichia coli is part of the normal microflora of the intestines of mammals. However, among the enteric pathogens, it is one of the leading causes of intestinal diseases, especially Shiga toxigenic E. coli, which can cause diarrhoea, haemorrhagic colitis and complications like haemolytic uraemic syndrome ...

journal_title：Letters in applied microbiology

authors： Ribeiro LF,Barbosa MM,de Rezende Pinto F,Guariz CS,Maluta RP,Rossi JR,Rossi GA,Lemos MV,do Amaral LA

更新日期：2016-03-01 00:00:00

abstract：AIMS:To determine the effects of urine, a urease inhibitor and/or an odour-reducing antimicrobial compound, on zoonotic pathogens in swine manure slurries. METHODS AND RESULTS:Swine faeces were collected and blended with different amounts of urine. Marker strains of Salmonella enterica subsp. enterica serovar Typhimur...

journal_title：Letters in applied microbiology

authors： Wells JE,Varel VH

更新日期：2008-04-01 00:00:00

abstract：UNLABELLED:Fast microbial identification is becoming increasingly necessary in industry to improve microbial control and reduce biocide consumption. We compared the performances of two systems based on MALDI-TOF MS (VITEK MS and BIOTYPER) and two based on biochemical testing (BIOLOG, VITEK 2 Compact) with genetic metho...

journal_title：Letters in applied microbiology

authors： Urwyler SK,Glaubitz J

更新日期：2016-02-01 00:00:00

abstract：AIMS:To investigate the use of mannitol salt agar (MSA) supplemented with acriflavine for selective growth and quantification of Staphylococcus aureus from flushed dairy manure wastewater (FDMW). METHODS AND RESULTS:Minimal inhibitory concentrations of acriflavine in MSA were determined by comparing the growth of S. a...

journal_title：Letters in applied microbiology

authors： Davis JA,Farrah SR,Wilkie AC

更新日期：2006-06-01 00:00:00

abstract：:Heat stable antifungal factor (HSAF) is considered to be a potential biological pesticide due to its broad antifungal activity and novel mode of action. However, few studies have reported on HSAF production during fermentation. Thus, this work was executed to optimize the medium composition to maximize HSAF production...

journal_title：Letters in applied microbiology

authors： Tang B,Zhao YC,Shi XM,Xu HY,Zhao YY,Dai CC,Liu FQ

更新日期：2018-05-01 00:00:00

abstract：AIMS:To develop an assay for rapid screening of bacterial adhesion to various groups of biomolecules present in fish mucus. METHODS AND RESULTS:A novel assay was developed for investigation of bacterial adhesion to various groups of mucus biomolecules from fish. Lipid-, protein-, carbohydrate- and nucleic acid-rich co...

journal_title：Letters in applied microbiology

authors： Tkachenko A,Da Silva L,Hearne J,Parveen S,Waguespack Y

更新日期：2013-01-01 00:00:00

abstract：AIM:To estimate the prevalence of thermotolerant Campylobacter spp. in commercially reared partridges (Perdix perdix) in southern Italy. METHODS AND RESULTS:Cloacal swabs of partridges (n = 240), equally distributed between male and female birds, from a game bird farm located in the Southern Italy were examined for th...

journal_title：Letters in applied microbiology

authors： Dipineto L,Gargiulo A,De Luca Bossa LM,Rinaldi L,Borrelli L,Santaniello A,Menna LF,Fioretti A

更新日期：2009-09-01 00:00:00

abstract：AIMS:Our goal was to develop a mathematical kinetic model to predict the sporicidal activity of glutaraldehyde, which is an active ingredient frequently used in commercial products employed for liquid disinfection and decontamination. METHODS AND RESULTS:We used our previously published data on spore inactivation by g...

journal_title：Letters in applied microbiology

authors： Retta SM,Sagripanti JL

更新日期：2008-05-01 00:00:00

abstract：AIMS:To determine whether nonspore-forming biothreat agents can survive and persist in potable water that does not contain a disinfectant. METHODS AND RESULTS: Autoclaved, de-chlorinated Atlanta municipal water was inoculated with eight isolates of bacterial biothreat agents (10⁶ CFU ml⁻¹). The inoculated water sample...

journal_title：Letters in applied microbiology

authors： Gilbert SE,Rose LJ

更新日期：2012-09-01 00:00:00

abstract：AIMS:Overproduced alpha-amylases in Bacillus subtilis provoke a specific stress response involving the CssRS two-component system, which controls expression of the HtrA and HtrB proteases. Previously, the B. subtilis TepA protein was implicated in high-level alpha-amylase secretion. Our present studies were aimed at in...

journal_title：Letters in applied microbiology

authors： Westers H,Darmon E,Zanen G,Veening JW,Kuipers OP,Bron S,Quax WJ,van Dijl JM

更新日期：2004-01-01 00:00:00

abstract：:Nineteen strains of vancomycin-resistant Enterococcus faecium isolated from 10 of 75 (13.3%) tenderloin beef samples were examined for resistance to selected antibiotics, presence of plasmids, and genetic diversity by random amplification of polymorphic DNA analysis. All strains showed multiple resistant to the antibi...

journal_title：Letters in applied microbiology

authors： Son R,Nimita F,Rusul G,Nasreldin E,Samuel L,Nishibuchi M

更新日期：1999-08-01 00:00:00

abstract：:High performance thin layer chromatography with automated multiple development was used to determine 2,3-butanediol levels in wine produced by high and low acetoin-forming strains of Saccharomyces cerevisiae. An inverse correlation between acetoin and 2,3-butanediol content was found suggesting a leaky mutation in ace...

journal_title：Letters in applied microbiology

authors： Romano P,Suzzi G,Brandolini V,Menziani E,Domizio P

更新日期：1996-04-01 00:00:00

abstract：:From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown or...

journal_title：Letters in applied microbiology

authors： Ahrens P,Friis NF

更新日期：1991-06-01 00:00:00

abstract：:Natural enzyme inhibitors have been widely described in literature because of its pharmacological and cosmetic applications. Fungi found in caves represent a promising source of bioactive substances that are still little explored scientifically. Thus, the present work evaluated the presence of enzymatic modulators in ...

journal_title：Letters in applied microbiology

authors： Cardoso MGB,Trento MVC,Reis CH,Marcussi S,Cardoso PG

更新日期：2019-08-01 00:00:00

abstract：:The effect of liquid mechanical vibration on the adhesion of Saccharomyces cerevisiae cells to the internal glass surface of a pipette was studied using a 25 Hz vibration source. The maximum vibration amplitude was 1.06 mm (peak to peak) along the pipette direction. Relative movements between the pipette and yeast sus...

journal_title：Letters in applied microbiology

authors： Jiang C,Yu H,Ding Z,Ma Y

更新日期：1996-07-01 00:00:00

abstract：AIMS:To study the cellular growth and morphology of Yarrowia lipolytica W29 and its lipase and protease production under increased air pressures. METHODS AND RESULTS:Batch cultures of the yeast were conducted in a pressurized bioreactor at 4 and 8 bar of air pressure and the cellular behaviour was compared with cultur...

journal_title：Letters in applied microbiology

authors： Lopes M,Gomes N,Gonçalves C,Coelho MA,Mota M,Belo I

更新日期：2008-02-01 00:00:00

abstract：AIM:To stimulate poly-beta-hydroxybutyrate (PHB) accumulation in Synechocystis sp. PCC 6803 by manipulating culture conditions. METHODS AND RESULTS:Stationary phase cultures of Synechocystis sp. PCC 6803 were subjected to N- and P-deficiency, chemoheterotrophy and limitations of gas-exchange. Enhanced PHB accumulation...

journal_title：Letters in applied microbiology

authors： Panda B,Mallick N

更新日期：2007-02-01 00:00:00

abstract：AIMS:  This paper utilized quantitative LC-MS/MS to profile the short-chain acyl-CoA levels of several strains of Escherichia coli engineered for heterologous polyketide production. To further compare and potentially expand the levels of available acyl-CoA molecules, a propionyl-CoA synthetase gene from Ralstonia solan...

journal_title：Letters in applied microbiology

authors： Armando JW,Boghigian BA,Pfeifer BA

更新日期：2012-02-01 00:00:00

abstract：:Staphylococcus epidermidis and Staphylococcus aureus, two bacterial strains commonly associated with biofilm-related medical infections and food poisoning, can rapidly colonize biotic and abiotic surfaces. The present study investigates the ability of anodic alumina surfaces with nanoporous surface topography to minim...

journal_title：Letters in applied microbiology

authors： Feng G,Cheng Y,Worobo RW,Borca-Tasciuc DA,Moraru CI

更新日期：2019-10-01 00:00:00

abstract：:PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni a...

journal_title：Letters in applied microbiology

authors： Rasmussen HN,Olsen JE,Jørgensen K,Rasmussen OF

更新日期：1996-11-01 00:00:00

abstract：:Quillaja saponin (QS) was incubated at 39 degrees C in an in vitro medium containing rumen liquor from a cow fed a roughage diet. No degradation of QS was observed up to 6 h of fermentation. Incubation for 9, 12 and 24 h decreased the content of QS by 16%, 45% and 100%. The content of QS did not decrease when incubate...

journal_title：Letters in applied microbiology

authors： Makkar HP,Becker K

更新日期：1997-10-01 00:00:00

abstract：AIM:To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture. METHODS AND RESULTS:A Matlab algorithm was developed for the estimation of model parameters....

journal_title：Letters in applied microbiology

authors： Aguilar-Uscanga MG,Garcia-Alvarado Y,Gomez-Rodriguez J,Phister T,Delia ML,Strehaiano P

更新日期：2011-08-01 00:00:00

abstract：UNLABELLED:Blue light (470 nm) LED antimicrobial properties were studied alone against bacteria and with or without the food grade photosensitizer, erythrosine (ERY) against filamentous fungi. Leuconostoc mesenteroides (LM), Bacillus atrophaeus (BA) or Pseudomonas aeruginosa (PA) aliquots were exposed on nutrient agar ...

journal_title：Letters in applied microbiology

authors： De Lucca AJ,Carter-Wientjes C,Williams KA,Bhatnagar D

更新日期：2012-12-01 00:00:00

abstract：AIMS:To test the inhibitory activity of 2-nitro-1-propanol (2NPOH) against Salmonella Typhimurium, Escherichia coli O157:H7 and Enterococcus faecalis. METHODS AND RESULTS:Specific growth rates (h(-1)) of S. Typhimurium, E. coli O157:H7 and Ent. faecalis were determined during culture in tryptic soya broth (TSB) supple...

journal_title：Letters in applied microbiology

authors： Jung YS,Anderson RC,Callaway TR,Edrington TS,Genovese KJ,Harvey RB,Poole TL,Nisbet DJ

更新日期：2004-01-01 00:00:00

abstract：UNLABELLED:Healthcare-associated infections (HCAI) affect 5-10% of acute hospital admissions. Environmental decontamination is an important component of all strategies to prevent HCAI as many bacterial causes survive and persist in the environment, which serve as ongoing reservoirs of infection. Current approaches such...

journal_title：Letters in applied microbiology

authors： Galvin S,Cahill O,O'Connor N,Cafolla AA,Daniels S,Humphreys H

更新日期：2013-08-01 00:00:00

abstract：:Species of Aspergillus section Nigri are not easily distinguished by traditional morphological techniques, and typically are identified by DNA sequencing methods. We developed four PCR primers to distinguish between Aspergillus niger, Aspergillus welwitschiae, Aspergillus carbonarius and Aspergillus tubingensis, based...

journal_title：Letters in applied microbiology

authors： Palumbo JD,O'Keeffe TL

更新日期：2015-02-01 00:00:00

abstract：:Chronic Pseudomonas aeruginosa infection is associated with increased morbidity and mortality in patients with cystic fibrosis (CF). Current understanding of risk factors for acquisition is limited and so the aim of this study was to examine a large sample of environmental waters from diverse sources. Environmental wa...

journal_title：Letters in applied microbiology

authors： Caskey S,Stirling J,Moore JE,Rendall JC

更新日期：2018-06-01 00:00:00

abstract：:The genus Taylorella is composed of two species: (i) Taylorella equigenitalis, the causative agent of CEM, a venereally transmitted infection of Equidae and (ii) Taylorella asinigenitalis, a closely related species considered to be nonpathogenic, although experimental infection of mares with this bacterium resulted in...

journal_title：Letters in applied microbiology

authors： Hébert L,Rincé I,Sanna C,Laugier C,Rincé A,Petry S

更新日期：2014-10-01 00:00:00

abstract：:Campylobacter jejuni was inoculated into a range of raw and ready-to-eat foods. These food samples were used as a test source for detection of this bacterium by the polymerase chain reaction (PCR). Specific detection of Camp. jejuni, as indicated by a PCR product of 159 bp, was possible with all pre-cooked deli-sliced...

journal_title：Letters in applied microbiology

authors： Winters DK,O'Leary AE,Slavik MF

更新日期：1998-09-01 00:00:00