Detection of Campylobacter jejuni and Camp. coli in chicken faecal samples by PCR.

Abstract:

:PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni and Camp. coli. The assay was used to analyse 31 chicken faecal samples. Full correspondence was found between the PCR assay conducted on the enriched cultures and the standard culture method. When analysing the transport medium prior to enrichment, the PCR assay detected nine of 11 culture positive samples.

journal_name

Lett Appl Microbiol

authors

Rasmussen HN,Olsen JE,Jørgensen K,Rasmussen OF

doi

10.1111/j.1472-765x.1996.tb00209.x

subject

Has Abstract

pub_date

1996-11-01 00:00:00

pages

363-6

issue

5

eissn

0266-8254

issn

1472-765X

journal_volume

23

pub_type

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