Abstract：
AIMS: This paper utilized quantitative LC-MS/MS to profile the short-chain acyl-CoA levels of several strains of Escherichia coli engineered for heterologous polyketide production. To further compare and potentially expand the levels of available acyl-CoA molecules, a propionyl-CoA synthetase gene from Ralstonia solanacearum (prpE-RS) was synthesized and expressed in the engineered strain BAP1. METHODS AND RESULTS: Upon feeding propionate, the engineered E. coli strains had increased the levels of both propionyl- and methylmalonyl-CoA of 6- to 30-fold and 3·7- to 6·8-fold, respectively. Expression of prpE-RS resulted in no significant increases in acetyl-, butyryl- and propionyl-CoA when fed the corresponding substrates (sodium acetate, butyrate or propionate). More interesting, however, were the results from strain BAP1 engineered for native prpE overexpression, which indicated increases in the same range of acyl-CoA formation. CONCLUSIONS: The increased acyl-CoA levels across the strains profiled in this study reflect the genetic modifications implemented for improved polyketide production and also indicate flexibility of the native PrpE. SIGNIFICANCE AND IMPACT OF THE STUDY: The results provide direct evidence of enhanced acyl-CoA levels correlating to those strains engineered for polyketide biosynthesis. This information and the inherent flexibility of the native PrpE enzyme support future efforts to characterize, engineer and extend acyl-CoA precursor supply for additional heterologous biosynthetic attempts.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Armando JW,Boghigian BA,Pfeifer BAdoi
10.1111/j.1472-765X.2011.03184.xsubject
Has Abstractpub_date
2012-02-01 00:00:00pages
140-8issue
2eissn
0266-8254issn
1472-765Xjournal_volume
54pub_type
杂志文章abstract：:Results of biocide efficacy testing on laboratory-generated microbial biofilms containing Legionella bozemanii is presented. These show that chlorine is effective at recommended concentrations in cooling towers; at least 48 h contact between free chlorine and the biofilm is necessary. Of five commercial biocides teste...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1993.tb00384.x
更新日期：1993-10-01 00:00:00
abstract：AIM:The mechanisms of adaptation of Clostridium perfringens to high temperatures are not well understood. In this work, the involvement of extracellular compounds in protection to heat was determined. METHODS AND RESULTS:Cells were grown in fluid thioglycollate medium or chicken broth. When mid-log phase was reached, ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2008.02504.x
更新日期：2009-01-01 00:00:00
abstract：AIMS:To compare the effects of five yeast probiotic formulations on viability, revival and washout kinetic in the digestive tract of mice, and the protection against an experimental infection with Salmonella enterica serovar Typhimurium. METHODS AND RESULTS:The number of viable cells in five commercial probiotic produ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2009.02732.x
更新日期：2009-12-01 00:00:00
abstract：AIM:The biotransformation of pentoxifylline (PTX), propentofylline (PPT) and their racemic hydroxy metabolites ((+/-)-OHPTX and (+/-)-OHPPT) by using the fungus Cunninghamella echinulata NRRL 1384. METHODS AND RESULTS:A fungus Cunninghamella echinulata NRRL 1384 was used to catalyse the (S)-selective oxidation of the ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2008.02478.x
更新日期：2009-01-01 00:00:00
abstract：AIMS:To investigate the diversity and dissemination of tetracycline resistance genes in isolates from estuarine waters. METHODS AND RESULTS:Forty-two out of 164 multi-resistant isolates previously obtained were resistant or less-susceptible to tetracycline, as evaluated by the disc diffusion method. Minimal inhibitory...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2008.02452.x
更新日期：2008-12-01 00:00:00
abstract：AIMS:This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples. METHODS AND RESULTS:A total of 285 stool samples were inoculated onto Chr...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2008.02517.x
更新日期：2009-02-01 00:00:00
abstract：:PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni a...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1996.tb00209.x
更新日期：1996-11-01 00:00:00
abstract：:The emergence of an increasing number of antibiotic resistant human clinical bacteria has been a great cause of concern for the last decades. As an example, Staphylococcus aureus isolates in the hospital environment are becoming more and more resistant to antibiotics including vancomycin which is considered as a last ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章,评审
doi：10.1111/j.1472-765X.2008.02490.x
更新日期：2009-01-01 00:00:00
abstract：:The marine antimicrobial peptide NZ17074, a variant of arenicin-3 from Arenicola marina that has broad antimicrobial activity and high bioavailability, can be designed to treat bacterial and fungal diseases. To reduce the toxicity of NZ17074, N6 was designed by replacing a cysteine in positions 3 and 20 with alanine, ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12770
更新日期：2017-09-01 00:00:00
abstract：UNLABELLED:Four strains were isolated from a spoiled whiting (Merlangius merlangus). All of them were able to grow aerobically from 4 to 30°C and also able to develop anaerobically in the presence of trimethylamine N-Oxide (TMAO) at 25°C. Biochemical characterization did not allow identification of the strains species ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12312
更新日期：2014-11-01 00:00:00
abstract：AIMS:To investigate the growth of salmonellae on sprouting alfalfa seeds as affected by the inoculum size, microbial load and Pseudomonas fluorescens 2-79. METHODS AND RESULTS:Alfalfa seeds pre-inoculated with < or =10(1)-10(3) CFU g(-1) of salmonellae and with or without Ps. fluorescens 2-79 were sprouted in glass ja...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2007.02302.x
更新日期：2008-02-01 00:00:00
abstract：AIM:The study aimed to evaluate the effect of the bacteriocins produced by Lactobacillus sakei CWBI-B1365 and Lactobacillus curvatus CWBI-B28 on the growth and survival of Listeria monocytogenes in raw beef and poultry meat. METHODS AND RESULTS:The sakacin P and sakacin G structural genes were identified in Lact. curv...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2008.02468.x
更新日期：2008-12-01 00:00:00
abstract：:Three white rot fungi were compared for their ability to attack polychlorinated biphenyl (PCB) congeners in the presence and absence of the non-ionic Triton X-100 or the anionic Dowfax 8390 surfactants at half their critical micelle concentrations. Neither surfactant affected PCB biodegradation monitored by gas chroma...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2000.00700.x
更新日期：2000-02-01 00:00:00
abstract：AIMS:In this study, we compared different methods of colistin susceptibility testing, disc diffusion, agar dilution and Etest using a set of Enterobacteriaceae isolates that included colistin-resistant strains. METHODS AND RESULTS:Susceptibility of 200 clinical isolates of Enterobacteriaceae to colistin was tested to ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2011.03145.x
更新日期：2011-11-01 00:00:00
abstract：AIMS:This study was conducted to clarify the taxonomic status of Bacteroides type A strains with high vitamin B(12)-producing ability that is widely distributed in the intestinal tracts of freshwater fish. METHODS AND RESULTS:Seventeen strains of Bacteroides type A isolated from five fish species were all rod-shaped a...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2007.02258.x
更新日期：2008-01-01 00:00:00
abstract：AIMS:To immobilize Methylobacterium sp. NP3 and Acinetobacter sp. PK1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol. METHODS AND RESULTS:The phenol degradation activity of suspended and immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 bacteria wa...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2011.03019.x
更新日期：2011-05-01 00:00:00
abstract：AIMS:To characterize and identify a novel contaminant of aviation fuel. METHODS AND RESULTS:Micro-organisms (yeasts and bacteria) were isolated from samples of aviation fuel. A yeast that proved to have been unrecorded previously was isolated from more than one fuel sample. This novel yeast proved to be a new species ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2010.02968.x
更新日期：2011-01-01 00:00:00
abstract：:Coliphages and enteroviruses were monitored over 12 months in sewage and air adjacent to an activated sludge plant. Both showed temporal variation but the mean count of phages in enterovirus-positive samples was not significantly different from that in enterovirus-negative samples. Hence coliphages are not necessarily...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1995.tb01042.x
更新日期：1995-09-01 00:00:00
abstract：:Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different tr...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1996.tb01110.x
更新日期：1996-01-01 00:00:00
abstract：AIMS:The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. METHODS AND RESULTS:Antimicrobial and ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2010.02818.x
更新日期：2010-04-01 00:00:00
abstract：:Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world. Coffee leaf blight, caused by Phomopsis heveicola, is one of the most important fungal diseases dangerous to coffee crops in China. This study aimed to develop a PCR-...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13396
更新日期：2020-09-26 00:00:00
abstract：:Live shellfish samples (120) were collected from nine littoral sites in Brittany (western France). They were screened for Listeria spp. and a count of faecal coliforms was carried out. Analysis of the results revealed Listeria spp. in 55% of samples, a much higher rate than the previous, infrequent, recorded data. Fur...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1998.00319.x
更新日期：1998-03-01 00:00:00
abstract：AIMS:To investigate the use of mannitol salt agar (MSA) supplemented with acriflavine for selective growth and quantification of Staphylococcus aureus from flushed dairy manure wastewater (FDMW). METHODS AND RESULTS:Minimal inhibitory concentrations of acriflavine in MSA were determined by comparing the growth of S. a...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.01915.x
更新日期：2006-06-01 00:00:00
abstract：:Synthesis of gold nanoparticles (AuNPs) has become a needed domain of applied science. Biological method for synthesis of AuNPs by Trichoderma hamatum SU136 aqueous mycelial extract was achieved. The culture filtrate of the fungus was exposed to three different concentrations of gold chloride. The culture filtrate of ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13055
更新日期：2018-11-01 00:00:00
abstract：AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2001.00928.x
更新日期：2001-06-01 00:00:00
abstract：:Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13061
更新日期：2018-11-01 00:00:00
abstract：AIMS:To evaluate the in vitro bactericidal activity of the novel antimicrobial peptide (AP) CECT7121 against Gram-positive bacteria from mastitic dairy cattle. METHODS AND RESULTS:A total of 15 Staphylococcus aureus, 10 Streptococcus dysgalactiae, 7 Strep. uberis, 1 Strep. agalactiae strains were isolated from 33 diff...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2008.02507.x
更新日期：2009-02-01 00:00:00
abstract：AIMS:To test the inhibitory activity of 2-nitro-1-propanol (2NPOH) against Salmonella Typhimurium, Escherichia coli O157:H7 and Enterococcus faecalis. METHODS AND RESULTS:Specific growth rates (h(-1)) of S. Typhimurium, E. coli O157:H7 and Ent. faecalis were determined during culture in tryptic soya broth (TSB) supple...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2004.01613.x
更新日期：2004-01-01 00:00:00
abstract：:Chronic Pseudomonas aeruginosa infection is associated with increased morbidity and mortality in patients with cystic fibrosis (CF). Current understanding of risk factors for acquisition is limited and so the aim of this study was to examine a large sample of environmental waters from diverse sources. Environmental wa...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12876
更新日期：2018-06-01 00:00:00
abstract：:Pinellia ternata is a traditional Chinese herb that has been shown to suffer from continuous cropping. Deterioration in continuous P. ternata cropping soils and the effect of alteration of crop rotation on the soil were evaluated by using Illumina high-throughput sequencing technology. Soil physicochemical properties ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13135
更新日期：2019-06-01 00:00:00
