Tetracycline-resistance genes in gram-negative isolates from estuarine waters.

Abstract:

AIMS:To investigate the diversity and dissemination of tetracycline resistance genes in isolates from estuarine waters. METHODS AND RESULTS:Forty-two out of 164 multi-resistant isolates previously obtained were resistant or less-susceptible to tetracycline, as evaluated by the disc diffusion method. Minimal inhibitory concentration for resistant bacteria ranged from 16 to 256 mg l(-1). Screening of tet genes by polymerase chain reaction showed that 88% of the isolates carried at least one of the genes tested, namely tet(A) (present in 13 isolates), tet(B) (present in 13 isolates), tet(C) (present in 3 isolates), tet(D) (present in 1 isolate), tet(E) (present in 6 isolates) and tet(M) (present in 1 isolate). One isolate carried tet(A) and tet(M). To our knowledge, this study presents the first description of a tet(D) gene in Morganella morganii. Hybridization revealed that tet genes were plasmid-located in 31% of the isolates. Those isolates were included as donors in conjugation experiments and 38% transferred tetracycline resistance. CONCLUSIONS:A considerable diversity of tet genes was detected in the estuary. Frequently, these genes were associated with plasmids and could be transferred to Escherichia coli. SIGNIFICANCE AND IMPACT OF THE STUDY:The results presented provide further evidence of the role played by estuarine reservoirs in antibiotic resistance maintenance and dissemination.

journal_name

Lett Appl Microbiol

authors

Henriques IS,Fonseca F,Alves A,Saavedra MJ,Correia A

doi

10.1111/j.1472-765X.2008.02452.x

subject

Has Abstract

pub_date

2008-12-01 00:00:00

pages

526-33

issue

6

eissn

0266-8254

issn

1472-765X

pii

LAM2452

journal_volume

47

pub_type

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