Genetic characterization and antimicrobial resistance of Staphylococcus aureus isolated from bovine milk in Tunisia.

Abstract:

:Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses in dairy industry worldwide. In addition, milk and milk-products contaminated by Staph. aureus can cause harmful human diseases. The aim of this study was to characterize Staph. aureus strains isolated from dairy farms in Tunisia. Bulk tank milk (n = 32) and individual cow milk (n = 130) samples were collected during the period of 2013-2014. Forty-three Staph. aureus isolates were recovered and typed by spa typing, 16S-23S rRNA intergenic spacer (RS-PCR) and multiplex PCRs for 22 virulence genes. Antimicrobial resistance was also investigated with a disc diffusion test. A selected subsample of 22 strains was additionally genotyped by multilocus sequence typing. Seventeen spa types were recovered, and t2421 (n = 10), t521 (n = 6) and t2112 (n = 5) were the most common. Fourteen different RS-PCR genotypes grouped into 11 clusters were detected in our study, with predominance of the RVI genotype (n = 24). Eight sequence types were identified and Clonal Complex 97, corresponding to RS-PCR cluster R, was the most common (n = 10), followed by CC1 (n = 4), CC15 (n = 3) and other four accounting for one or two strains. Different combinations of virulence genes were reported, and enterotoxin genes were present in few strains (seh, n = 4; sea, n = 2; sea and seh, n = 2; sec and sel, n = 2). The majority of strains were resistant only to penicillin; only one strain was found to be multiresistant and no methicillin-resistant Staph. aureus was demonstrated. Our study reported the isolation of CC97 from bovine milk in Tunisia for the first time and confirmed the relevance of this lineage in intramammary infection in cows. SIGNIFICANCE AND IMPACT OF THE STUDY:This paper describes the characteristics of Staphylococcus aureus isolated from bulk tank and individual cow milk in Tunisia. All strains were genotyped by spa typing and RS-PCR, a method based on the amplification of the 16S-23S rRNA intergenic spacer region, and multiplex PCRs for 22 virulence genes. A selected subsample of strains was also genotyped by multilocus sequence typing. All strains were tested for antimicrobial resistance. Our study evidences a predominance of strains belonging to Clonal Complex 97. Methicillin-resistant strains were not detected, and overall low level of antimicrobial resistance was reported.

journal_name

Lett Appl Microbiol

authors

Ben Said M,Abbassi MS,Bianchini V,Sghaier S,Cremonesi P,Romanò A,Gualdi V,Hassen A,Luini MV

doi

10.1111/lam.12672

subject

Has Abstract

pub_date

2016-12-01 00:00:00

pages

473-481

issue

6

eissn

0266-8254

issn

1472-765X

journal_volume

63

pub_type

杂志文章
  • Detecting a novel Eriocheir sinensis reovirus by reverse transcription loop-mediated isothermal amplification assay.

    abstract::The novel Eriocheir sinensis reovirus (EsRV) is a pathogen that causes severe disease and high mortality rates in cultivated crabs. Here, we established a highly sensitive and specific rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that was cheaper and more suitable for field applic...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12630

    authors: Ma Y,Dai T,Serwadda A,Shen H

    更新日期:2016-11-01 00:00:00

  • A novel assay for the distribution of pyrithione biocides in bacterial cells.

    abstract::Sodium pyrithione and zinc pyrithione (NaPT and ZnPT, respectively) are widely used as cosmetic preservatives and metal chelating agents. They are commonly assayed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). However, a simple quantitative colorimetric assay has not been pre...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00376.x

    authors: Dinning AJ,al-Adham IS,Austin P,Collier PJ

    更新日期:1998-07-01 00:00:00

  • Response surface methodology for optimizing the fermentation medium of alpha-galactosidase in solid-state fermentation.

    abstract:AIMS:Alpha-galactosidase is applied in food and feed industries for hydrolysing raffinose series oligosaccharides (RO) that are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. The objective of the current work was to develop an optimal culture medium for the production of al...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02173.x

    authors: Liu CQ,Chen QH,Tang B,Ruan H,He GQ

    更新日期:2007-08-01 00:00:00

  • In vitro growth control of selected pathogens by Lactobacillus acidophilus- and Lactobacillus casei-fermented milk.

    abstract:AIMS:Food-borne pathogen inhibition was tested in the presence of a mixture of Lactobacillus acidophilus and Lactobacillus casei during fermentation under controlled pH conditions. METHODS AND RESULTS:The growth of Escherichia coli O157:H7, Salmonella serotype Typhimurium, Staphylococcus aureus, Listeria innocua, Ente...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.02060.x

    authors: Millette M,Luquet FM,Lacroix M

    更新日期:2007-03-01 00:00:00

  • Genetic and phylogenetic evidence for misidentification of Vibrio species within the Harveyi clade.

    abstract:AIM:  This report describes the use of a six-gene multi-locus sequence analysis (MLSA) to correctly identify Vibrio strains of the Harveyi clade. METHODS AND RESULTS:  Vibrio isolates were characterized using a six housekeeping gene MLSA. The study provided evidence supporting: (i) a substantial number of reference st...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03183.x

    authors: Hoffmann M,Monday SR,Fischer M,Brown EW

    更新日期:2012-02-01 00:00:00

  • Nanobacteria--propagating calcifying nanoparticles.

    abstract::Nanobacteria, also known as calcifying nanoparticles (CNP), are controversial infectious agents not matching the current criteria for 'living organism'. Despite the controversy of their classification, they propagate and cause cell death in vitro and are associated or found in many human diseases. Thus, more efforts s...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1472-765X.2006.01945.x

    authors: Kajander EO

    更新日期:2006-06-01 00:00:00

  • Electronic paramagnetic resonance investigation of the activity of Origanum vulgare L. essential oil on the Listeria monocytogenes membrane.

    abstract:AIMS:To evaluate the effect of oregano essential oil on Listeria monocytogenes cytoplasmic membrane. METHODS AND RESULTS:Nitroxide free-radical Electron Paramagnetic Resonance was applied on L. monocytogenes after 30 min exposure to oregano essential oil concentrations ranging from 0 to 1.25%. The impact of essential ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02877.x

    authors: Serio A,Chiarini M,Tettamanti E,Paparella A

    更新日期:2010-08-01 00:00:00

  • Determination of 2,3-butanediol in high and low acetoin producers of Saccharomyces cerevisiae wine yeasts by automated multiple development (AMD).

    abstract::High performance thin layer chromatography with automated multiple development was used to determine 2,3-butanediol levels in wine produced by high and low acetoin-forming strains of Saccharomyces cerevisiae. An inverse correlation between acetoin and 2,3-butanediol content was found suggesting a leaky mutation in ace...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb01165.x

    authors: Romano P,Suzzi G,Brandolini V,Menziani E,Domizio P

    更新日期:1996-04-01 00:00:00

  • Growth inhibition and antioxidative response of wood decay fungi exposed to plant extracts of Casearia species.

    abstract:UNLABELLED:Ligninolytic fungi take part in critical processes in ecosystems such as nutrient recycling; however, some fungal species can be pathogenic to forest and urban trees and deteriorate wood products. The tropical flora is an important source of antimicrobial compounds environmentally safer than traditional wood...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12159

    authors: Bento TS,Torres LM,Fialho MB,Bononi VL

    更新日期:2014-01-01 00:00:00

  • Decontamination of dental unit water systems with hydrogen peroxide.

    abstract:AIMS:Transmission of microbial pathogens to patients from water in dental units is a concern. To reduce this risk, the decontaminating efficiency of hydrogen peroxide was evaluated. METHODS AND RESULTS:Three percent hydrogen peroxide diluted 1 : 4 in distilled water (contact time 15 min) was used daily to disinfect th...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01378.x

    authors: Zanetti F,De Luca G,Tarlazzi P,Stampi S

    更新日期:2003-01-01 00:00:00

  • Possible link between a 35 kDa membrane protein and osmolyte transport in Staphylococcus aureus.

    abstract::Accumulation of proline and glycine betaine occurs via three transport systems in Staphylococcus aureus (Pourkomailian and Booth 1992, 1994). A 35 kDa membrane protein was identified to be missing from a mutant, BP108, which lacks activity of the low affinity osmolyte transport system. The loss in activity of the low ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00306.x

    authors: Pourkomaillan B

    更新日期:1998-02-01 00:00:00

  • A lack of predatory interaction between rumen ciliate protozoa and Shiga-toxin producing Escherichia coli.

    abstract:AIMS:To investigate interactions between rumen protozoa and Shiga toxin-producing Escherichia coli (STEC) and to ascertain whether it is likely that rumen protozoa act as ruminant hosts for STEC. METHODS AND RESULTS:The presence of stx genes in different microbial fractions recovered from cattle and sheep rumen conten...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2004.01642.x

    authors: Burow LC,Gobius KS,Vanselow BA,Klieve AV

    更新日期:2005-01-01 00:00:00

  • Cold storage affects survival and growth of Erwinia amylovora on the calyx of apple.

    abstract:AIMS:To determine the effect of cold storage on the survival of Erwinia amylovora. METHODS AND RESULTS:The survival of E. amylovora was assessed during storage at 2 degrees C. Populations of E. amylovora inoculated into phosphate-buffered saline remained static, whereas in nutrient media populations increased at low t...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01407.x

    authors: Taylor RK,Hale CN

    更新日期:2003-01-01 00:00:00

  • Mechanical characterization of yeast cells: effects of growth conditions.

    abstract:UNLABELLED:Industrial biotechnology uses microbiological cells to produce a wide range of products. While the organisms in question are well understood regarding their genetic and molecular properties, less is known about their mechanical properties. Previous work has established a testing procedure for single Saccharo...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12468

    authors: Overbeck A,Kampen I,Kwade A

    更新日期:2015-10-01 00:00:00

  • An evaluation of five preservation techniques and conventional freezing temperatures of -20 degrees C and -85 degrees C for long-term preservation of Campylobacter jejuni.

    abstract:AIMS:This study aimed to identify a simple, inexpensive preservation technique that will allow a quick and reliable recovery of Campylobacter jejuni following long-term periods of preservation. METHODS AND RESULTS:Preservation techniques include (i) Cryobank microbial preservation system using hypertonic 'cryopreserva...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.2004.01490.x

    authors: Gorman R,Adley CC

    更新日期:2004-01-01 00:00:00

  • Optimization of culture conditions for production of antimalarial menisporopsin A by the seed fungus Menisporopsis theobromae BCC 4162.

    abstract:AIMS:The aim of this work was to optimize the production of a novel antimaralial menisporopsin A by the seed fungus Menisporopsis theobromae BCC 4162. METHODS AND RESULTS:Fungal cultures were grown in shake flasks at 25 degrees C in the basal medium with varying carbon and nitrogen sources, aeration rates and initial ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2006.01994.x

    authors: Madla S,Kittakoop P,Wongsa P

    更新日期:2006-11-01 00:00:00

  • Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps militaris.

    abstract:AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00950.x

    authors: Park JP,Kim SW,Hwang HJ,Yun JW

    更新日期:2001-07-01 00:00:00

  • Evaluation of sample preparation methods for MALDI-TOF MS identification of highly dangerous bacteria.

    abstract:AIMS:To propose a universal workflow of sample preparation method for the identification of highly pathogenic bacteria by MALDI-TOF MS. METHODS AND RESULTS:Fifteen bacterial species, including highly virulent Gram-positive (Bacillus anthracis and Clostridium botulinum) and Gram-negative bacteria (Brucella melitensis, ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2012.03255.x

    authors: Drevinek M,Dresler J,Klimentova J,Pisa L,Hubalek M

    更新日期:2012-07-01 00:00:00

  • Development and evaluation of a new device to effectively detach micro-organisms from food samples.

    abstract:AIMS: To develop a new instrument of great versatility for recovering micro-organisms from all types of food samples and to compare the effects with existing sample preparation methods. METHODS AND RESULTS: To detach micro-organisms from large-size unbroken food samples such as apples, carrots, potatoes and tomatoes w...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2012.03289.x

    authors: Kim YH,Lee SY,Sagong HG,Heu S,Ryu S,Kang DH

    更新日期:2012-09-01 00:00:00

  • Faecal contamination of echinoderms: first report of heavy Escherichia coli loading of sea urchins from a natural growing area.

    abstract:UNLABELLED:Although little evidence existed to support that view, European countries and in particular France, have regarded echinoderms, including sea urchins, as low risk in terms of feacal contamination. It is hypothesized that the sea urchins mode of feeding, which is based on grazing and differs from bivalve mollu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12524

    authors: Bouchoucha M,Piquet JC,Chavanon F,Dufresne C,Le Guyader FS

    更新日期:2016-02-01 00:00:00

  • Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish.

    abstract:AIMS:This study was conducted to clarify the taxonomic status of Bacteroides type A strains with high vitamin B(12)-producing ability that is widely distributed in the intestinal tracts of freshwater fish. METHODS AND RESULTS:Seventeen strains of Bacteroides type A isolated from five fish species were all rod-shaped a...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02258.x

    authors: Tsuchiya C,Sakata T,Sugita H

    更新日期:2008-01-01 00:00:00

  • Isolation of a strain of Bacillus thuringiensis ssp. kurstaki HD-1 encoding delta-endotoxin Cry1E.

    abstract::A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00355.x

    authors: Chang JH,Roh JY,Je YH,Park HW,Jin BR,Woo SD,Kang SK

    更新日期:1998-05-01 00:00:00

  • Species identification, virulence markers and antimicrobial resistance profiles of Aeromonas sp. isolated from marketed hard-shelled mussel (Mytilus coruscus) in Korea.

    abstract::Hard-shelled mussel (Mytilus coruscus) is a popular seafood in Korea. This study aimed to determine the virulence markers and antimicrobial resistance patterns of 33 Aeromonas strains isolated from mussels. The isolates were identified as A. salmonicida (n = 14), A. veronii (n = 9), A. enteropelogenes (n = 4), A. cavi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13266

    authors: Hossain S,Wickramanayake MVKS,Dahanayake PS,Heo GJ

    更新日期:2020-03-01 00:00:00

  • Cell-wall protein profiles of dairy thermophilic lactobacilli.

    abstract::SDS-PAGE fingerprinting of cell-wall proteins extracted from 119 strains belonging to different species of lactic acid bacteria have been compared. The method of extraction and electrophoretic separation utilized in this work was found to be a reliable and rapid way for characterizing thermophilic lactobacilli species...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00235.x

    authors: Gatti M,Fornasari E,Neviani E

    更新日期:1997-11-01 00:00:00

  • Incidence of Listeria spp. in Breton live shellfish.

    abstract::Live shellfish samples (120) were collected from nine littoral sites in Brittany (western France). They were screened for Listeria spp. and a count of faecal coliforms was carried out. Analysis of the results revealed Listeria spp. in 55% of samples, a much higher rate than the previous, infrequent, recorded data. Fur...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00319.x

    authors: Monfort P,Minet J,Rocourt J,Piclet G,Cormier M

    更新日期:1998-03-01 00:00:00

  • Isolation of Actinomyces viscosus strain GA: characteristics of its cellular biological retardant(s).

    abstract::Actinomyces viscosus strain GA produces an exocellular biological retardant(s) that prevents certain vegetable plants from becoming overgrown. The biological retardant(s) was assayed using the etiolated wheat coleoptile assay, and fractionation of culture supernatant fluid resulted in a partial purification of the ret...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00178.x

    authors: Bryant FO,Cutler HG

    更新日期:1997-08-01 00:00:00

  • Design of a species-specific PCR method for the detection of the heat-resistant fungi Talaromyces macrosporus and Talaromyces trachyspermus.

    abstract::Heat-resistant fungi occur sporadically and are a continuing problem for the food and beverage industry. The genus Talaromyces, as a typical fungus, is capable of producing the heat-resistant ascospores responsible for the spoilage of processed food products. Isocitrate lyase, a signature enzyme of the glyoxylate cycl...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12818

    authors: Yamashita S,Nakagawa H,Sakaguchi T,Arima TH,Kikoku Y

    更新日期:2018-01-01 00:00:00

  • Overexpression of Ecm22 improves ergosterol biosynthesis in Saccharomyces cerevisiae.

    abstract::Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13061

    authors: Wang SQ,Wang T,Liu JF,Deng L,Wang F

    更新日期:2018-11-01 00:00:00

  • Candida keroseneae sp. nov., a novel contaminant of aviation kerosene.

    abstract:AIMS:To characterize and identify a novel contaminant of aviation fuel. METHODS AND RESULTS:Micro-organisms (yeasts and bacteria) were isolated from samples of aviation fuel. A yeast that proved to have been unrecorded previously was isolated from more than one fuel sample. This novel yeast proved to be a new species ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02968.x

    authors: Buddie AG,Bridge PD,Kelley J,Ryan MJ

    更新日期:2011-01-01 00:00:00

  • Effect of penicillin G on the electroporation of Rhodococcus rhodochrous CF222.

    abstract::Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different tr...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb01110.x

    authors: Sunairi M,Iwabuchi N,Murakami K,Watanabe F,Ogawa Y,Murooka H,Nakajima M

    更新日期:1996-01-01 00:00:00