Abstract:
:The novel Eriocheir sinensis reovirus (EsRV) is a pathogen that causes severe disease and high mortality rates in cultivated crabs. Here, we established a highly sensitive and specific rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that was cheaper and more suitable for field applications in crab aquaculture than those of traditional reverse transcription-polymerase chain reaction (RT-PCR) analysis. The amplification was completed within 45 min under isothermal conditions at 65°C. The RT-LAMP test for EsRV had a detection limit of 15 pg, and sensitivity was 100 times greater than that of conventional RT-PCR. The LAMP primers for EsRV were not amplified by other pathogen strains, indicating good specificity. In addition to detection by electrophoresis, RT-LAMP results were detectable by visual observations of reaction tube turbidity, and calcein was added to visually detect the amplification products. These results indicate that this highly convenient, rapid and sensitive RT-LAMP assay can be used to detect EsRV-infected aquatic organisms. SIGNIFICANCE AND IMPACT OF THE STUDY:Tremor disease (TD) is one of the most serious diseases of Eriocheir sinensis. A novel E. sinensis reovirus (EsRV) was identified from E. sinensis afflicted with TD and caused high mortality. We developed a reverse transcription loop-mediated isothermal amplification assay with high specificity, sensitivity and rapidity to detect EsRV, which can be used to diagnose aquatic animal diseases, particularly where expensive diagnostic instruments are not available.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Ma Y,Dai T,Serwadda A,Shen Hdoi
10.1111/lam.12630subject
Has Abstractpub_date
2016-11-01 00:00:00pages
363-368issue
5eissn
0266-8254issn
1472-765Xjournal_volume
63pub_type
杂志文章abstract::The aim of this study was to determine the epidemiology (location, microbial load, microbiome, presence/absence of biofilm and pathogens, including ESKAPE-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, and antimicrobial susc...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13127
更新日期:2019-04-01 00:00:00
abstract:AIMS:2-Keto-4-methylthiobutyric acid (KMBA) is an established intermediate in microbial biosynthesis of ethylene from methionine. This study demonstrates the kinetics and effects of trace elements and electron complexes on substrate (KMBA)-derived C2H4 biosynthesis in soil. METHODS AND RESULTS:We have previously repor...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01590.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To develop a PCR-based method for quantitative detection of Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) in wheat seeds. METHODS AND RESULTS:Based on the sequences of the cyp51A gene, two primer pairs FaF + FaR and FgF + FgR were developed for the species-specific detection of Fa and Fg, respectively. To...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02595.x
更新日期:2009-06-01 00:00:00
abstract:AIMS:To develop and evaluate a real-time PCR assay incorporating an internal amplification control (IAC) suitable for the screening of Shiga toxin (Stx)-producing Escherichia coli (STEC) in foods. METHODS AND RESULTS:A competitive IAC was constructed and included in an stx-specific real-time PCR assay. Coupled to 18-h...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02561.x
更新日期:2009-05-01 00:00:00
abstract::Accumulation of proline and glycine betaine occurs via three transport systems in Staphylococcus aureus (Pourkomailian and Booth 1992, 1994). A 35 kDa membrane protein was identified to be missing from a mutant, BP108, which lacks activity of the low affinity osmolyte transport system. The loss in activity of the low ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.00306.x
更新日期:1998-02-01 00:00:00
abstract:AIMS: Lesions of DNA are removed by nucleotide excision repair (NER) process in the living systems. NER process-related host factors are believed to aid recovery steps during viral integration. Here, we report identification and characterization of a DNA repair molecule Rad23 from kuruma shrimp Marsupenaeus japonicus. ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03073.x
更新日期:2011-07-01 00:00:00
abstract::PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni a...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb00209.x
更新日期:1996-11-01 00:00:00
abstract::The small cryptic plasmid pMB1 (1.9 kb), previously isolated from Bifidobacterium longum, has been characterized by physical mapping. Two cloning vectors, pMR3 and pDG7, carrying chloramphenicol and ampicillin resistances derived from pJH101, have been electroporated in Escherichia coli. ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1990.tb00165.x
更新日期:1990-10-01 00:00:00
abstract::Sodium pyrithione and zinc pyrithione (NaPT and ZnPT, respectively) are widely used as cosmetic preservatives and metal chelating agents. They are commonly assayed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). However, a simple quantitative colorimetric assay has not been pre...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.00376.x
更新日期:1998-07-01 00:00:00
abstract::Phorate [O,O-diethyl-S-(ethylthio)methyl phosphoradiothioate] degrading bacteria were isolated from agricultural soil and characterized based on their morphological and biochemical characteristics. The selected isolates PS-1, PS-2 and PS-3 were presumptively identified as Rhizobium, Pseudomonas and Proteous species, r...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01329.x
更新日期:2003-01-01 00:00:00
abstract::Fattening pigs are often infected with campylobacter. To eliminate campylobacter from the pig population, a top-down approach, involving the breeding and reproduction farms, seems appropriate. In order to investigate the effectiveness of a top-down approach, sows' faeces from the following farms were analysed for the ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00750.x
更新日期:2000-06-01 00:00:00
abstract:AIMS:The aims of this study are to obtain the draft genome sequence of Streptomyces coelicoflavus ZG0656, which produces novel acarviostatin family α-amylase inhibitors, and then to reveal the putative acarviostatin-related gene cluster and the biosynthetic pathway. METHODS AND RESULTS:The draft genome sequence of S. ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2012.03274.x
更新日期:2012-08-01 00:00:00
abstract::Coliphages and enteroviruses were monitored over 12 months in sewage and air adjacent to an activated sludge plant. Both showed temporal variation but the mean count of phages in enterovirus-positive samples was not significantly different from that in enterovirus-negative samples. Hence coliphages are not necessarily...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb01042.x
更新日期:1995-09-01 00:00:00
abstract::Recent studies have shown that Salmonella shedding status affects sows' microbiota during gestation and that these modifications are reflected in the faecal microbiota of their piglets at weaning. The aims of this study were: (a) to evaluate the persistence, up to the fattening period, of the previously measured link ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13406
更新日期:2021-02-01 00:00:00
abstract:AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00950.x
更新日期:2001-07-01 00:00:00
abstract:AIM:To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture. METHODS AND RESULTS:A Matlab algorithm was developed for the estimation of model parameters....
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03081.x
更新日期:2011-08-01 00:00:00
abstract::Control of biofouling on seawater reverse osmosis (SWRO) membranes is a major challenge as treatments can be expensive, damage the membrane material and often biocides do not remove the polymers in which bacteria are embedded. Biological control has been largely ignored for biofouling control. The objective of this st...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12747
更新日期:2017-07-01 00:00:00
abstract::A Coxiella sp. closely related to the agent of Q-fever, Coxiella burnetii, has been associated with mortalities in redclaw crayfish, (Cherax quadricarinatus), in farms and experimental facilities for three decades. Limited sequence data including 16S rRNA have placed the rickettsial species as a new species, candidatu...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13336
更新日期:2020-09-01 00:00:00
abstract::Enterohaemorrhagic Escherichia coli (EHEC) agar was evaluated for its ability to recover one isolate of each of three serotypes (O157:H7, O26 and O113:H21) of shiga toxin-producing E. coli (STEC) from raw mince, pasteurized milk and salami after enrichment. The method detected around one colony-forming unit (cfu) in 2...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00676.x
更新日期:2000-02-01 00:00:00
abstract:AIMS:Rapid detection of pathogenic Yersinia enterocolitica isolates by using antisera raised against recombinant attachment-invasion locus (Ail) protein. METHODS AND RESULTS:The complete gene (471 bp) encoding for the Ail protein was amplified by PCR and cloned in pQE 30 UA vector. The recombinant clones were selected...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02755.x
更新日期:2010-02-01 00:00:00
abstract:AIMS:This work was carried out to determine if there was a difference in the microbial population of the rumen associated with daylength at which sheep are housed. METHODS AND RESULTS:Denaturing gradient gel electrophoresis (DGGE) was used to study the ciliate and bacterial diversity in the rumen of Soay rams kept in ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2005.01707.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination. METHODS AND RESULTS:Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences wer...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01948.x
更新日期:2006-09-01 00:00:00
abstract:AIMS:To develop a sensitive, rapid and simple method for detection of Botrytis cinerea based on loop-mediated isothermal amplification (LAMP) that would be suitable for use outside a conventional laboratory setting. METHODS AND RESULTS:A LAMP assay was designed based on the intergenic spacer of the B. cinerea nuclear ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02949.x
更新日期:2010-12-01 00:00:00
abstract:AIMS:The aim of this work was to optimize the production of a novel antimaralial menisporopsin A by the seed fungus Menisporopsis theobromae BCC 4162. METHODS AND RESULTS:Fungal cultures were grown in shake flasks at 25 degrees C in the basal medium with varying carbon and nitrogen sources, aeration rates and initial ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01994.x
更新日期:2006-11-01 00:00:00
abstract:UNLABELLED:Syphilis is a multistage sexually transmitted disease that remains a serious public health concern worldwide. The coexistence of Treponema pallidum with other closely related members of spirochaeta, such as Leptospira spp. and Borrelia burgdorferi, has complicated the serodiagnosis due to cross-reactive anti...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12554
更新日期:2016-04-01 00:00:00
abstract::Blown pack spoilage (BPS) of vacuum packaged beef is caused by psychrotolerant and psychrophilic Clostridium species, primarily Clostridium estertheticum and Clostridium gasigenes. The aim of this study was to investigate the environmental niches and impact of season on these BPS Clostridium spp. on Irish beef farms. ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13344
更新日期:2020-12-01 00:00:00
abstract:UNLABELLED:High-pressure homogenization (HPH) has been proposed to be applied directly to lactic acid bacterial cells at sublethal levels to enhance some functional properties. As the principal target of HPH are the cell surface envelope structures, the aim of this work was to study the effect of a HPH treatment, appli...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12164
更新日期:2014-02-01 00:00:00
abstract::A total of 87 isolates of Yersinia enterocolitica were examined with randomly amplified polymorphic DNA (RAPD) by use of three different primers. Based on the RAPD profiles, the strains could be divided into three major groups: (1) the pathogenic American serotypes, O:8, O:13ab, O:20 and O:21; (2) the pathogenic Europ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1994.tb00475.x
更新日期:1994-11-01 00:00:00
abstract:AIM:To test the efficacy of a chemical (chlorous acid) for reducing the numbers of viable Alicyclobacillus acidoterrestris spores in laboratory media and on apples. METHODS AND RESULTS:Alicyclobacillus acidoterrestris spores in aqueous suspension and on apple surfaces of four different cultivars were treated with 268 ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02874.x
更新日期:2010-08-01 00:00:00
abstract:AIMS:The aims of this study were to investigate in vitro the effects of Lactobacillus isolates from a chicken on adhesion of pathogenic Salmonella and Escherichia coli to chicken intestinal mucus obtained from different intestinal regions. METHODS AND RESULTS:Bacteria were labelled by using methyl-1,2-[(3)H]-thymidine...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01844.x
更新日期:2006-04-01 00:00:00