Genetic diversity and expression profiles of cysteine phytases in the sheep rumen during a feeding cycle.

abstract：AIMS:This work was carried out to study the acid production by Lactococcus lactis subsp. lactis strains isolated from goat's milk and goat cheese (Valdeteja variety) in order to select a suitable starter culture for industrial goat cheese manufacturing. METHODS AND RESULTS:The titrable acidity of 45 Lactococcus lactis...

journal_title：Letters in applied microbiology

authors： Alonso-Calleja C,Carballo J,Capita R,Bernardo A,García-López ML

更新日期：2002-01-01 00:00:00

abstract：:For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain...

journal_title：Letters in applied microbiology

authors： Yang M,Mu T,Zhong W,Olajuyin AM,Xing J

更新日期：2017-08-01 00:00:00

abstract：AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designin...

journal_title：Letters in applied microbiology

authors： Mukhopadhyay HK,Amsaveni S,Matta SL,Antony PX,Thanislass J,Pillai RM

更新日期：2012-09-01 00:00:00

abstract：AIMS:We propose to apply the Wirtz-Conklin staining technique to evaluate spore germination. METHODS AND RESULTS:Spores at different stages of germination were stained with modified spore stain (Wirtz-Conklin) and evaluated for staining properties. Bacillus spores suspended in deionized water, which does not support g...

journal_title：Letters in applied microbiology

authors： Hamouda T,Shih AY,Baker JR Jr

更新日期：2002-01-01 00:00:00

abstract：AIMS:To screen for the existence and determine the structure of Tn916-like element in Streptococcus parauberis serotype II strains isolated from cultured Japanese flounder in western Japan. METHODS AND RESULTS:In this study, the structure of Tn916-like element and the flanking regions were characterized by polymerase ...

journal_title：Letters in applied microbiology

authors： Meng F,Kanai K,Yoshikoshi K

更新日期：2009-06-01 00:00:00

abstract：AIMS:The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS:Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cult...

journal_title：Letters in applied microbiology

authors： Eyigor A,Carli KT,Unal CB

更新日期：2002-01-01 00:00:00

abstract：:Two commercially available screening methods, an automated enzyme-linked fluorescent immunoassay (VIDAS E. coli O157) and an immunomagnetic separation followed by culture onto cefixime tellurite sorbitol MacConkey agar (CT-SMAC), were compared for detection of Escherichia coli O157 in naturally and artificially contam...

journal_title：Letters in applied microbiology

authors： Vernozy-Rozand C,Mazuy C,Ray-Gueniot S,Boutrand-Loeï S,Meyrand A,Richard Y

更新日期：1997-12-01 00:00:00

abstract：AIM:To identify the gene that encodes nigrescin, a bacteriocin produced by Prevotella nigrescens ATCC 25261. METHODS AND RESULTS:Each open reading frame (ORF) of the nig gene cluster (nigA, nigB, nigC and nigD) was transferred into an expression vector. The recombinant proteins encoded by nigA, nigB, nigC and nigD wer...

journal_title：Letters in applied microbiology

authors： Teanpaisan R,Narawatthana S,Utarabhand P

更新日期：2009-09-01 00:00:00

abstract：:The effect of relative humidity (RH) on the antimicrobial efficacy of chlorine dioxide (ClO2 ) gas against foodborne pathogens on tomatoes was evaluated. Also, levels of ClO2 residues on tomatoes after exposure to ClO2 gas under different RH conditions were measured to determine the quantity of solubilized ClO2 gas on...

journal_title：Letters in applied microbiology

authors： Park SH,Kim WJ,Kang DH

更新日期：2018-08-01 00:00:00

abstract：AIMS:To characterize and identify a novel contaminant of aviation fuel. METHODS AND RESULTS:Micro-organisms (yeasts and bacteria) were isolated from samples of aviation fuel. A yeast that proved to have been unrecorded previously was isolated from more than one fuel sample. This novel yeast proved to be a new species ...

journal_title：Letters in applied microbiology

authors： Buddie AG,Bridge PD,Kelley J,Ryan MJ

更新日期：2011-01-01 00:00:00

abstract：:Enterococci were isolated from faecal droppings of chickens in broiler and layer farms and the susceptibilities to nine therapeutic antimicrobial agents and six growth-promoting antibiotics were determined by the agar dilution method. Resistance to therapeutic antimicrobial agents such as ampicillin, clindamycin, eryt...

journal_title：Letters in applied microbiology

authors： Yoshimura H,Ishimaru M,Endoh YS,Kojima A

更新日期：2000-12-01 00:00:00

abstract：:Vacuum-packaged poultry cooked sausages were pressure-treated at 500 MPa by combinations of time (5-45 min) and temperature (2-80 degrees C) and later stored at 6-8 degrees C for 12 we. Mesophile and psychrotrophe counts were determined 1 d, 3, 6, 9 and 12 we after treatment and compared with those of cooked sausages ...

journal_title：Letters in applied microbiology

authors： Yuste J,Mor-Mur M,Capellas M,Pla R

更新日期：1999-10-01 00:00:00

abstract：AIMS: To develop a new instrument of great versatility for recovering micro-organisms from all types of food samples and to compare the effects with existing sample preparation methods. METHODS AND RESULTS: To detach micro-organisms from large-size unbroken food samples such as apples, carrots, potatoes and tomatoes w...

journal_title：Letters in applied microbiology

authors： Kim YH,Lee SY,Sagong HG,Heu S,Ryu S,Kang DH

更新日期：2012-09-01 00:00:00

abstract：:Three white rot fungi were compared for their ability to attack polychlorinated biphenyl (PCB) congeners in the presence and absence of the non-ionic Triton X-100 or the anionic Dowfax 8390 surfactants at half their critical micelle concentrations. Neither surfactant affected PCB biodegradation monitored by gas chroma...

journal_title：Letters in applied microbiology

authors： Beaudette LA,Ward OP,Pickard MA,Fedorak PM

更新日期：2000-02-01 00:00:00

abstract：AIMS:To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming. METHODS AND RESULTS:The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously con...

journal_title：Letters in applied microbiology

authors： Dong H,Tao W,Zhu L,Zhang Y,Li Y

更新日期：2011-09-01 00:00:00

abstract：UNLABELLED:Cryptococcosis, a fungal infection that affects both immunocompromised and immunocompetent individuals, contributes to increasing indices of mortality and morbidity. The development of resistance by Cryptococcus spp., the limited number of commercial antifungal drugs and the various side effects of these dru...

journal_title：Letters in applied microbiology

authors： Magalhães TF,da Silva CM,de Fátima Â,da Silva DL,Modolo LV,Martins CV,Alves RB,Ruiz AL,Longato GB,de Carvalho JE,de Resende-Stoianoff MA

更新日期：2013-08-01 00:00:00

abstract：:Adhesion of three Lactobacillus strains onto human epithelial intestinal Caco-2 and Int-407 cell lines was compared. More adhesion occurred onto Int-407. The trypsin and sodium periodate pretreatment of bacteria revealed different mechanisms of adhesion depending on the Caco-2 and Int-407, involving carbohydrates and ...

journal_title：Letters in applied microbiology

authors： Sarem F,Sarem-Damerdji LO,Nicolas JP

更新日期：1996-06-01 00:00:00

abstract：:The use of buoyant density centrifugation (BDC) to prepare samples for PCR analysis of food pathogens is described. Blue cheese and milk homogenates were inoculated with Shigella flexneri and layered on top of Percoll media. After BDC, the food homogenates remained in the upper part of the centrifuge tube, separated f...

journal_title：Letters in applied microbiology

authors： Lindqvist R,Norling B,Lambertz ST

更新日期：1997-04-01 00:00:00

abstract：AIMS:To investigate interactions between rumen protozoa and Shiga toxin-producing Escherichia coli (STEC) and to ascertain whether it is likely that rumen protozoa act as ruminant hosts for STEC. METHODS AND RESULTS:The presence of stx genes in different microbial fractions recovered from cattle and sheep rumen conten...

journal_title：Letters in applied microbiology

authors： Burow LC,Gobius KS,Vanselow BA,Klieve AV

更新日期：2005-01-01 00:00:00

abstract：UNLABELLED:The unicellular freshwater green microalga Haematococcus pluvialis is the richest source of natural astaxanthin. Since accumulation of astaxanthin differs significantly among various algal strains at different stages, it is therefore critical to develop an effective high-throughput assay for rapid screening ...

journal_title：Letters in applied microbiology

authors： Liu JH,Song L,Huang Q

更新日期：2016-02-01 00:00:00

abstract：AIMS:In this study, we report a new, simple methodology for the monitoring of Cryptosporidium oocysts and Giardia cysts in drinking water samples, ranging from 10- to 1000-l, which combines a new ARAD microfibre filtration of the (oo)cysts from drinking water and loop-mediated isothermal amplification (LAMP) of a human...

journal_title：Letters in applied microbiology

authors： Plutzer J,Törökné A,Karanis P

更新日期：2010-01-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are polyesters accumulated by many bacteria under unbalanced growth conditions, and have been used to meet the various demands in areas of agriculture, medicine, and materials especially belong to a rapidly expanding area of biomedical research. Unfortunately, the high production cost than...

journal_title：Letters in applied microbiology

authors： Liu CC,Zhang LL,An J,Chen B,Yang H

更新日期：2016-01-01 00:00:00

abstract：AIMS:The objective of this study was to subtype Arcobacter butzleri isolates using RAPD-PCR. METHODS AND RESULTS:Thirty-five A. butzleri isolates obtained from chicken carcasses were examined. PCR-mediated DNA fingerprinting technique with primers of the variable sequence motifs was used to detect polymorphism within ...

journal_title：Letters in applied microbiology

authors： Atabay HI,Bang DD,Aydin F,Erdogan HM,Madsen M

更新日期：2002-01-01 00:00:00

abstract：:A Coxiella sp. closely related to the agent of Q-fever, Coxiella burnetii, has been associated with mortalities in redclaw crayfish, (Cherax quadricarinatus), in farms and experimental facilities for three decades. Limited sequence data including 16S rRNA have placed the rickettsial species as a new species, candidatu...

journal_title：Letters in applied microbiology

authors： Ruth Elliman J,Owens L

更新日期：2020-09-01 00:00:00

abstract：:Fattening pigs are often infected with campylobacter. To eliminate campylobacter from the pig population, a top-down approach, involving the breeding and reproduction farms, seems appropriate. In order to investigate the effectiveness of a top-down approach, sows' faeces from the following farms were analysed for the ...

journal_title：Letters in applied microbiology

authors： Weijtens MJ,Urlings HA,Van der Plas J

更新日期：2000-06-01 00:00:00

abstract：AIMS:To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO(2) ) and dry heat in reducing the number of Escherichia coli O157:H7. METHODS AND RESULTS:Radish seeds containing E. coli O157:H7 at 5.5 log CFU g(-1) were treated with 500 μg ml(-1) ClO(2) for 5 min and subseq...

journal_title：Letters in applied microbiology

authors： Bang J,Kim H,Kim H,Beuchat LR,Kim Y,Ryu JH

更新日期：2011-10-01 00:00:00

abstract：AIMS:The gram-positive bacterial genus Lactococcus has been taxonomically classified into seven species (Lactococcus lactis, Lactococcus garvieae, Lactococcus piscium, Lactococcus plantarum, Lactococcus raffinolactis, Lactococcus chungangensis and Lactococcus fujiensis). This study aimed to develop a novel multiplex po...

journal_title：Letters in applied microbiology

authors： Odamaki T,Yonezawa S,Kitahara M,Sugahara Y,Xiao JZ,Yaeshima T,Iwatsuki K,Ohkuma M

更新日期：2011-05-01 00:00:00

abstract：:Protoplasts of Streptomyces flavopersicus with the highest regeneration frequency were isolated from late log phase mycelium grown in a two-stage culture system with 2% glycine in the medium. Of the 11 regeneration media tested, R9 was selected as the most efficient with 29 degrees C as the best temperature. ...

journal_title：Letters in applied microbiology

authors： Lyutzkanova D,Nikolova B,Stoilova-Discheva M,Todorov T

更新日期：1993-04-01 00:00:00

abstract：AIMS:The goal of the study was to develop a reliable, reproducible and rapid method of culture in order to screen a large number of fungal transformants. METHODS AND RESULTS:The method is based upon miniaturized cell cultures and automated expression screening in microwell plates. For the method development, 50 recomb...

journal_title：Letters in applied microbiology

authors： Alberto F,Navarro D,de Vries RP,Asther M,Record E

更新日期：2009-08-01 00:00:00

abstract：UNLABELLED:The structure and dynamics of microbial populations play a significant role during cheese manufacture and ripening. Therefore, fast and accurate methods for identification and characterization of the microbial populations are of fundamental importance to the cheese industry. In this study, we investigate the...

journal_title：Letters in applied microbiology

authors： Porcellato D,Brighton C,McMahon DJ,Oberg CJ,Lefevre M,Broadbent JR,Steele JL

更新日期：2014-08-01 00:00:00