Abstract:
UNLABELLED:The unicellular freshwater green microalga Haematococcus pluvialis is the richest source of natural astaxanthin. Since accumulation of astaxanthin differs significantly among various algal strains at different stages, it is therefore critical to develop an effective high-throughput assay for rapid screening astaxanthin-hyperproducing strains. In the present study, near-infrared spectroscopy (NIRS) in combination with biochemical assay was employed for evaluation of the wide-type H. Pluvialis strains. The partial least squares (PLS) models of total biomass, astaxanthin content and astaxanthin expressed as a percentage of dry weight (DW) were developed with the R(2) values as 0·959, 0·982 and 0·952, the prediction correlation factor (r) values as 0·979, 0·988 and 0·966, and the residual predictive deviation (RPD) values as 4·88, 6·22 and 3·86, respectively. Furthermore, the PLS models were employed to evaluate H. pluvialis mutants, with the r values as 0·973, 0·983 and 0·976, and the RPD values as 3·45, 7·59 and 4·07, respectively. This work thus demonstrates that NIRS is an easy, fast and non-invasive approach that can be applied in high-throughput screening of astaxanthin-hyperproducing algal mutants. SIGNIFICANCE AND IMPACT OF THE STUDY:Haematococcus pluvialis has potential application for its ability to accumulate natural antioxidant astaxanthin. In this study, we initiated the application of near-infrared spectroscopy (NIRS) in the analysis of total biomass and astaxanthin content of different mutant strains, demonstrating that NIRS can be very useful in the screening of axataxanthin-hyperproducing mutant strains.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Liu JH,Song L,Huang Qdoi
10.1111/lam.12531subject
Has Abstractpub_date
2016-02-01 00:00:00pages
185-91issue
2eissn
0266-8254issn
1472-765Xjournal_volume
62pub_type
杂志文章abstract::Microsomal chitinase from yeast and hyphal cells of Candida albicans was activated endogenously by incubation at 30 degrees C and exogenously by trypsin. The putative activating factor of yeast cells was separated from chitinase activity by fractionation of lysed protoplasts on an Iodixanol density gradient. The vacuo...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb00054.x
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journal_title:Letters in applied microbiology
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doi:10.1046/j.1472-765x.1997.00200.x
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2013-02-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02618.x
更新日期:2009-07-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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