Electroporation-induced transformation of Escherichia coli: evaluation of a square waveform pulse.

abstract：:Leclercia adecarboxylata, a Gram-negative bacillus of family Enterobacteriaceae, is an uncommonly identified pathogen isolated from environmental and clinical specimens. Most of the human infections are polymicrobial and commonly occur in immunocompromised hosts, although nosocomial infections in immunocompetent hosts...

journal_title：Letters in applied microbiology

authors： Choudhary M,Choudhary BK,Bhoyar S,Kale SB,Chaudhari SP,Bera BC,Jain A,Barbuddhe SB

更新日期：2018-01-01 00:00:00

abstract：:Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serot...

journal_title：Letters in applied microbiology

authors： Black SF,Gray DI,Fenlon DR,Kroll RG

更新日期：1995-03-01 00:00:00

abstract：AIMS:To investigate interactions between rumen protozoa and Shiga toxin-producing Escherichia coli (STEC) and to ascertain whether it is likely that rumen protozoa act as ruminant hosts for STEC. METHODS AND RESULTS:The presence of stx genes in different microbial fractions recovered from cattle and sheep rumen conten...

journal_title：Letters in applied microbiology

authors： Burow LC,Gobius KS,Vanselow BA,Klieve AV

更新日期：2005-01-01 00:00:00

abstract：:Enterohaemorrhagic Escherichia coli (EHEC) agar was evaluated for its ability to recover one isolate of each of three serotypes (O157:H7, O26 and O113:H21) of shiga toxin-producing E. coli (STEC) from raw mince, pasteurized milk and salami after enrichment. The method detected around one colony-forming unit (cfu) in 2...

journal_title：Letters in applied microbiology

authors： Hudson JA,Nicol C,Capill J,Bennett J

更新日期：2000-02-01 00:00:00

abstract：:The beta-D-galactosidase activity of viable but non-culturable (vnc) Escherichia coli cells in seawater was investigated using a rapid fluorimetric enzyme assay. Results from microcosm studies showed that loss of culturability did not necessarily result in loss of the ability to produce the galactosidase enzyme. Even ...

journal_title：Letters in applied microbiology

authors： Davies CM,Apte SC,Peterson SM

更新日期：1995-08-01 00:00:00

abstract：AIMS:To investigate fusion expression between Bacillus thuringiensis crystal protein and a foreign protein, the expression of a fusion protein comprised of Cry1Ac, and enhanced green fluorescent protein (EGFP) in B. thuringiensis Cry(-)B strain was examined. METHODS AND RESULTS:The N-terminal fusion expression of EGFP...

journal_title：Letters in applied microbiology

authors： Roh JY,Li MS,Chang JH,Choi JY,Shim HJ,Shin SC,Boo KS,Je YH

更新日期：2004-01-01 00:00:00

abstract：AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...

journal_title：Letters in applied microbiology

authors： Sun J,Hu XL,Le GW,Shi YH

更新日期：2010-03-01 00:00:00

abstract：AIMS:Our goal was to develop a mathematical kinetic model to predict the sporicidal activity of glutaraldehyde, which is an active ingredient frequently used in commercial products employed for liquid disinfection and decontamination. METHODS AND RESULTS:We used our previously published data on spore inactivation by g...

journal_title：Letters in applied microbiology

authors： Retta SM,Sagripanti JL

更新日期：2008-05-01 00:00:00

abstract：UNLABELLED:Industrial biotechnology uses microbiological cells to produce a wide range of products. While the organisms in question are well understood regarding their genetic and molecular properties, less is known about their mechanical properties. Previous work has established a testing procedure for single Saccharo...

journal_title：Letters in applied microbiology

authors： Overbeck A,Kampen I,Kwade A

更新日期：2015-10-01 00:00:00

abstract：UNLABELLED:Catfishes of the genus Panaque are known for their ability to feed on wood and hence to process cellulose fibres in their digestive systems. The paper industry uses cellulose fibres and thus has an interest in exploiting this property biomimetically: it could be employed as a pretreatment to lessen the energ...

journal_title：Letters in applied microbiology

authors： Di Maiuta N,Schwarzentruber P,Schenker M,Schoelkopf J

更新日期：2013-06-01 00:00:00

abstract：:Nicotinic acid (NA) is ubiquitous in nature and its microbial degradation mechanisms are diverse. In this study, Pusillimonas sp. strain T2 was found to be capable of utilizing NA as sole carbon and nitrogen sources. This strain could completely degrade 300 mg l-1 NA within 3·5 h at 30°C and pH 7·0 and one of the degr...

journal_title：Letters in applied microbiology

authors： Yuan M,Zhang Y,Zhao L,Ma Y,He Q,He J,Qiu J

更新日期：2018-04-01 00:00:00

abstract：AIMS:The development of a simple, rapid and sensitive method for the screening of chitinase inhibitors using Ostazin Brilliant Red labelled chitin as a substrate for chitinase assay. METHODS AND RESULTS:The colloidal substrate prepared from the Ostazin Brilliant Red labelled chitin was used in the estimation of chitin...

journal_title：Letters in applied microbiology

authors： Shirazi F,Kulkarni M,Deshpande MV

更新日期：2007-06-01 00:00:00

abstract：:Synthesis of gold nanoparticles (AuNPs) has become a needed domain of applied science. Biological method for synthesis of AuNPs by Trichoderma hamatum SU136 aqueous mycelial extract was achieved. The culture filtrate of the fungus was exposed to three different concentrations of gold chloride. The culture filtrate of ...

journal_title：Letters in applied microbiology

authors： Abdel-Kareem MM,Zohri AA

更新日期：2018-11-01 00:00:00

abstract：:Bulk tank milk from 160 producers was tested for Listeria monocytogenes at three monthly intervals over 1 year. Twenty-five producers were positive, most on a single occasion, only seven were positive on three or more of the four samplings. Listeria monocytogenes numbers were low, usually < 1 ml-1, the highest was 35 ...

journal_title：Letters in applied microbiology

authors： Fenlon DR,Stewart T,Donachie W

更新日期：1995-01-01 00:00:00

abstract：:White Spot Syndrome Virus (WSSV) is a dsDNA virus causing White Spot Syndrome Disease (WSSD) in shrimp with almost 100% morality rate within 3-10 days. In Bangladesh, WSSD is one of the major impediments of shrimp farming. This study first investigated the prevalence and distribution of WSSV in cultured shrimps of the...

journal_title：Letters in applied microbiology

authors： Hossain A,Nandi SP,Siddique MA,Sanyal SK,Sultana M,Hossain MA

更新日期：2015-02-01 00:00:00

abstract：AIMS:To evaluate the effect of oregano essential oil on Listeria monocytogenes cytoplasmic membrane. METHODS AND RESULTS:Nitroxide free-radical Electron Paramagnetic Resonance was applied on L. monocytogenes after 30 min exposure to oregano essential oil concentrations ranging from 0 to 1.25%. The impact of essential ...

journal_title：Letters in applied microbiology

authors： Serio A,Chiarini M,Tettamanti E,Paparella A

更新日期：2010-08-01 00:00:00

abstract：AIMS:This study assessed the plant growth-promoting ability of the bacterial strains Ochrobactrum intermedium (isolate CrT-1) and Bacillus cereus (isolate S-6). METHODS AND RESULTS:Two chromium resistant bacterial strains isolated from chromium-contaminated wastewater and soils were identified as O. intermedium CrT-1 ...

journal_title：Letters in applied microbiology

authors： Faisal M,Hasnain S

更新日期：2006-10-01 00:00:00

abstract：:Amphotericin B (AmB), a typical polyene macrolide antifungal agent, is widely used to treat systemic mycoses. In the present study, we show that the fungicidal activity of AmB was enhanced by benzyl isothiocyanate (BITC), a cruciferous plant-derived compound, in the budding yeast, Saccharomyces cerevisiae. In addition...

journal_title：Letters in applied microbiology

authors： Yamada N,Murata W,Yamaguchi Y,Fujita KI,Ogita A,Tanaka T

更新日期：2020-10-31 00:00:00

abstract：AIMS:To study the viability of a culture of the rumen protozoon Entodinium caudatum after a cryopreservation procedure by a fluorescence microscopy staining method. METHODS AND RESULTS:Fluorescence method is based on the different colour of cells depending on their membrane integrity. When the temperature effect was s...

journal_title：Letters in applied microbiology

authors： de la Fuente G,Cebrián JA,Fondevila M

更新日期：2004-01-01 00:00:00

abstract：:A Coxiella sp. closely related to the agent of Q-fever, Coxiella burnetii, has been associated with mortalities in redclaw crayfish, (Cherax quadricarinatus), in farms and experimental facilities for three decades. Limited sequence data including 16S rRNA have placed the rickettsial species as a new species, candidatu...

journal_title：Letters in applied microbiology

authors： Ruth Elliman J,Owens L

更新日期：2020-09-01 00:00:00

abstract：UNLABELLED:Most of the current research into the quantification of soil-borne pathogenic oomycetes lacks determination of DNA extraction efficiency, probably leading to an incorrect estimation of DNA quantity. In this study, we developed a convenient method by using a 100 bp artificially synthesized DNA sequence derive...

journal_title：Letters in applied microbiology

authors： Li M,Ishiguro Y,Kageyama K,Zhu Z

更新日期：2015-08-01 00:00:00

abstract：:The genus Taylorella is composed of two species: (i) Taylorella equigenitalis, the causative agent of CEM, a venereally transmitted infection of Equidae and (ii) Taylorella asinigenitalis, a closely related species considered to be nonpathogenic, although experimental infection of mares with this bacterium resulted in...

journal_title：Letters in applied microbiology

authors： Hébert L,Rincé I,Sanna C,Laugier C,Rincé A,Petry S

更新日期：2014-10-01 00:00:00

abstract：AIMS:To determine the effect of interacting conditions of water activity (aw, 0.99-0.85), temperature (15, 25 degrees C) and time (40 days) on growth and production of the mycotoxins deoxynivalenol (DON) and nivalenol (NIV) by Fusarium culmorum on a wheat-based agar medium. METHODS AND RESULTS:Fusarium culmorum grew o...

journal_title：Letters in applied microbiology

authors： Hope R,Magan N

更新日期：2003-01-01 00:00:00

abstract：:A pilot survey of the counts of total coliform bacteria, thermotolerant coliform bacteria, Escherichia coli and faecal streptococci was carried out at eight inland recreational waters at weekly intervals during July 1991. The aims were to assess the feasibility of determining candidate indicators of recreational water...

journal_title：Letters in applied microbiology

authors：

更新日期：1995-10-01 00:00:00

abstract：:Polymerase chain reaction (PCR) was used after a short pre-enrichment culture to detect Salmonella subspecies in chicken fillets. A direct PCR assay performed with chicken meat inoculated with Salmonella Typhimurium produced no PCR products. Six different DNA extraction protocols were tested to recover efficiently Sal...

journal_title：Letters in applied microbiology

authors： Soumet C,Ermel G,Fach P,Colin P

更新日期：1994-11-01 00:00:00

abstract：UNLABELLED:Blue light (470 nm) LED antimicrobial properties were studied alone against bacteria and with or without the food grade photosensitizer, erythrosine (ERY) against filamentous fungi. Leuconostoc mesenteroides (LM), Bacillus atrophaeus (BA) or Pseudomonas aeruginosa (PA) aliquots were exposed on nutrient agar ...

journal_title：Letters in applied microbiology

authors： De Lucca AJ,Carter-Wientjes C,Williams KA,Bhatnagar D

更新日期：2012-12-01 00:00:00

abstract：UNLABELLED:Bioconversion of biomass, particularly crop wastes, into biofuels is being developed as an alternative approach in meeting the high energy demand. In this study, a thermophilic bacterial strain BY-3 that exhibits cellulolytic potential was isolated from faecal samples of Tibetan pigs; this strain was identif...

journal_title：Letters in applied microbiology

authors： Meng F,Ma L,Ji S,Yang W,Cao B

更新日期：2014-09-01 00:00:00

abstract：:To investigate the potential activation of hydrogen peroxide by a novel catalyst, reducing the concentration of hydrogen peroxide required and the time taken for mycobactericidal activity. The mycobactericidal properties of an iron-based novel heterogeneous-modified polyacrylonitrile (PAN) catalyst in combination with...

journal_title：Letters in applied microbiology

authors： Price SL,Huddersman KD,Shen J,Walsh SE

更新日期：2013-02-01 00:00:00

abstract：:Sixteen Bifidobacterium isolates from the human gastrointestinal tract were assayed for susceptibility to 44 antibiotics by soft agar overlay disc diffusion on TPY agar. Five isolates (3/7 B. bifidum and 2/3 B. breve) exhibited atypical antibiotic susceptibility profiles. Poor growth in the agar overlay accounted for ...

journal_title：Letters in applied microbiology

authors： Charteris WP,Kelly PM,Morelli L,Collins JK

更新日期：1998-05-01 00:00:00

abstract：AIM:To evaluate the presence of thermotolerant Campylobacter spp. in stray cats in southern Italy. METHODS AND RESULTS:One hundred and thirteen animals were trapped in two different environments (urban area, harbour area) of the city of Naples. From each cat, rectal swabs were collected. The samples were processed in ...

journal_title：Letters in applied microbiology

authors： Gargiulo A,Rinaldi L,D'Angelo L,Dipineto L,Borrelli L,Fioretti A,Menna LF

更新日期：2008-02-01 00:00:00