Preliminary study of microbiological parameters in eight inland recreational waters. Public Health Laboratory Service Water Surveillance Group.

abstract：:Colistin-resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high-resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known geno...

journal_title：Letters in applied microbiology

authors： Tahmasebi H,Dehbashi S,Arabestani MR

更新日期：2020-04-01 00:00:00

abstract：AIMS:The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. METHODS AND RESULTS:Viruses were eluted from digestive tissues ...

journal_title：Letters in applied microbiology

authors： Elamri DE,Aouni M,Parnaudeau S,Le Guyader FS

更新日期：2006-10-01 00:00:00

abstract：:The development of various types of virus-like particles (VLPs) has accelerated over the past two decades as the importance of VLPs for generating next-generation vaccines has been appreciated. Yeast has advantages such as scalable fermentation, low risk of contamination by adventitious agents, low production costs an...

journal_title：Letters in applied microbiology

authors： Kim HJ,Kim HJ

更新日期：2017-02-01 00:00:00

abstract：:Four strains of Escherichia coli were tested for electroporation-induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a si...

journal_title：Letters in applied microbiology

authors： Elvin S,Bingham AH

更新日期：1991-02-01 00:00:00

abstract：AIMS:To compare the effects of five yeast probiotic formulations on viability, revival and washout kinetic in the digestive tract of mice, and the protection against an experimental infection with Salmonella enterica serovar Typhimurium. METHODS AND RESULTS:The number of viable cells in five commercial probiotic produ...

journal_title：Letters in applied microbiology

authors： Martins FS,Veloso LC,Arantes RM,Nicoli JR

更新日期：2009-12-01 00:00:00

abstract：:The Crystal Enteric/Nonfermenter (E/NF) identification kit (Becton Dickinson Microbiology Systems, USA) was evaluated using water-derived bacterial isolates and results compared to those obtained by the API 20E system (BioMérieux, UK). Both the E/NF and 20E systems correctly identified 93% of the Enterobacteriaceae re...

journal_title：Letters in applied microbiology

authors： Micklewright IJ,Sartory DP

更新日期：1995-09-01 00:00:00

abstract：:The effect of relative humidity (RH) on the antimicrobial efficacy of chlorine dioxide (ClO2 ) gas against foodborne pathogens on tomatoes was evaluated. Also, levels of ClO2 residues on tomatoes after exposure to ClO2 gas under different RH conditions were measured to determine the quantity of solubilized ClO2 gas on...

journal_title：Letters in applied microbiology

authors： Park SH,Kim WJ,Kang DH

更新日期：2018-08-01 00:00:00

abstract：AIMS:Tuberculous pleurisy is an important cause of pleural effusions in areas with a high incidence of tuberculosis. In this study, we developed an IS1081-based LAMP for the detection of Mycobacterium tuberculosis complex and investigated its usefulness in the diagnosis of tuberculous pleurisy. METHODS AND RESULTS:Inv...

journal_title：Letters in applied microbiology

authors： Yang B,Wang X,Li H,Li G,Cao Z,Cheng X

更新日期：2011-11-01 00:00:00

abstract：AIM:The aim of this work was to evaluate the effect of Planococcus ficus infection in red wine grapes on Aspergillus section Nigri and ochratoxin A (OTA) contamination. METHODS AND RESULTS:During 2006/2007 and 2008/2009 vintages, Merlot, Malbec and Cabernet Sauvignon varieties divided into two categories of grape samp...

journal_title：Letters in applied microbiology

authors： Chiotta ML,Ponsone ML,Torres AM,Combina M,Chulze SN

更新日期：2010-08-01 00:00:00

abstract：AIMS:To investigate fusion expression between Bacillus thuringiensis crystal protein and a foreign protein, the expression of a fusion protein comprised of Cry1Ac, and enhanced green fluorescent protein (EGFP) in B. thuringiensis Cry(-)B strain was examined. METHODS AND RESULTS:The N-terminal fusion expression of EGFP...

journal_title：Letters in applied microbiology

authors： Roh JY,Li MS,Chang JH,Choi JY,Shim HJ,Shin SC,Boo KS,Je YH

更新日期：2004-01-01 00:00:00

abstract：AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in the vineyards where koshu grapes, a primary wine grape cultivar in Japan, are grown. METHODS AND RESULTS:Sixty samples, including leaves, undamaged grape berries and soil under damaged berries, were collected at four koshu vineyards in Yamanashi ...

journal_title：Letters in applied microbiology

authors： Yanagida F,Srionnual S,Chen YS

更新日期：2008-08-01 00:00:00

abstract：AIMS:The objective of the study was to evaluate the variability of germination response of 10 strains of proteolytic Clostridium botulinum. METHODS AND RESULTS:An automated turbidometric method was used to follow the fall in optical density. Spores of proteolytic Cl. botulinum germinated in response to l-alanine alone...

journal_title：Letters in applied microbiology

authors： Alberto F,Broussolle V,Mason DR,Carlin F,Peck MW

更新日期：2003-01-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：AIMS:To compare the bacteriostatic and bactericidal activity of 13 chemotyped essential oils (EO) on 65 bacteria with varying sensitivity to antibiotics. METHODS AND RESULTS:Fifty-five bacterial strains were tested with two methods used for evaluation of antimicrobial activity (CLSI recommendations): the agar dilution...

journal_title：Letters in applied microbiology

authors： Mayaud L,Carricajo A,Zhiri A,Aubert G

更新日期：2008-09-01 00:00:00

abstract：:The ability of chemotherapeutic agents to affect the growth of common bacterial pathogens and the relationship between the effects of chemotherapeutics and antimicrobials is largely unknown. The purpose of this study was to describe the susceptibility of canine bacterial isolates to chemotherapeutic agents and to comp...

journal_title：Letters in applied microbiology

authors： Campbell O,Gagnon J,Rubin JE

更新日期：2019-11-01 00:00:00

abstract：:RAPD technique (randomly amplified polymorphic DNA) was used for epidemiological subtyping of Bacillus licheniformis and other Bacillus spp. Within 46 isolates of B. licheniformis, up to 10 strain types could be determined when two 10-mer primers were used. RAPD patterns, which were found in eight further strains of B...

journal_title：Letters in applied microbiology

authors： Stephan R,Schraft H,Untermann F

更新日期：1994-05-01 00:00:00

abstract：:Cow's milk was inoculated with ca 10(3) and 10(7) cfu ml-1 Escherichia coli O157:H7. After fermentation at 42 degrees C for 0-5 h, the yoghurt was stored at 4 degrees C. Two kinds of yoghurt were used: traditional yoghurt (TY), made with Streptococcus thermophilus and Lactobacillus bulgaricus starter cultures, and 'bi...

journal_title：Letters in applied microbiology

authors： Massa S,Altieri C,Quaranta V,De Pace R

更新日期：1997-05-01 00:00:00

abstract：:The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...

journal_title：Letters in applied microbiology

authors： García-Ortega L,Lacadena J,Lacadena V,Masip M,De Antonio C,Martínez-Ruiz A,Martínez Del Pozo A

更新日期：2000-04-01 00:00:00

abstract：:SDS-PAGE fingerprinting of cell-wall proteins extracted from 119 strains belonging to different species of lactic acid bacteria have been compared. The method of extraction and electrophoretic separation utilized in this work was found to be a reliable and rapid way for characterizing thermophilic lactobacilli species...

journal_title：Letters in applied microbiology

authors： Gatti M,Fornasari E,Neviani E

更新日期：1997-11-01 00:00:00

abstract：AIMS:To develop and evaluate a real-time PCR assay incorporating an internal amplification control (IAC) suitable for the screening of Shiga toxin (Stx)-producing Escherichia coli (STEC) in foods. METHODS AND RESULTS:A competitive IAC was constructed and included in an stx-specific real-time PCR assay. Coupled to 18-h...

journal_title：Letters in applied microbiology

authors： Auvray F,Lecureuil C,Dilasser F,Taché J,Derzelle S

更新日期：2009-05-01 00:00:00

abstract：:The ability of Stenotrophomonas maltophilia strain VUN 10,003 to degrade and detoxify high molecular weight polycyclic aromatic hydrocarbons (PAHs) was evaluated in a basal liquid medium. Using high cell density inocula of strain VUN 10,003, the concentration of pyrene, fluoranthene, benz[a]anthracene, benzo[a]pyrene,...

journal_title：Letters in applied microbiology

authors： Juhasz AL,Stanley GA,Britz ML

更新日期：2000-05-01 00:00:00

abstract：UNLABELLED:This work aimed to study the interaction between Actinomycetal isolates and Aspergillus flavus to promote mutual antagonism in contact. Thirty-seven soilborn Streptomyces spp. isolates were chosen as potential candidates. After a 10-day in vitro co-incubation period, 27 isolates respond to the criteria, that...

journal_title：Letters in applied microbiology

authors： Verheecke C,Liboz T,Darriet M,Sabaou N,Mathieu F

更新日期：2014-06-01 00:00:00

abstract：UNLABELLED:The objective of this study was to evaluate the effect of different caffeine concentrations (0-4%) on (i) lag phase prior to growth, (ii) growth rates and (iii) ochratoxin A (OTA) production by strains from the Aspergillus section Circumdati and Aspergillus section Nigri groups, isolated from coffee, when gr...

journal_title：Letters in applied microbiology

authors： Akbar A,Medina A,Magan N

更新日期：2016-07-01 00:00:00

abstract：AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designin...

journal_title：Letters in applied microbiology

authors： Mukhopadhyay HK,Amsaveni S,Matta SL,Antony PX,Thanislass J,Pillai RM

更新日期：2012-09-01 00:00:00

abstract：:Nisin, a bacteriocin produced by some strains of Lactococcus lactis, acts against foodborne pathogen Listeria monocytogenes. A single exposure of cells to nisin can generate nisin-resistant (Nisr) mutants, which may compromise the use of nisin in the food industry. The objective of this research was to compare the hea...

journal_title：Letters in applied microbiology

authors： Modi KD,Chikindas ML,Montville TJ

更新日期：2000-03-01 00:00:00

abstract：AIMS:Enzyme kinetics of purified laccases from six different Pleurotus ostreatus strains were determined in the oxidation of syringaldazine, guaiacol and ABTS. METHODS AND RESULTS:Significant differences in the kinetic constants were found. Catalytic activity (kcat) ranged from 19 to 941 U mg(-1) for syringaldazine, f...

journal_title：Letters in applied microbiology

authors： Tinoco R,Pickard MA,Vazquez-Duhalt R

更新日期：2001-05-01 00:00:00

abstract：AIMS:Alpha-galactosidase is applied in food and feed industries for hydrolysing raffinose series oligosaccharides (RO) that are the factors primarily responsible for flatulence upon ingestion of soybean-derived products. The objective of the current work was to develop an optimal culture medium for the production of al...

journal_title：Letters in applied microbiology

authors： Liu CQ,Chen QH,Tang B,Ruan H,He GQ

更新日期：2007-08-01 00:00:00

abstract：UNLABELLED:In this study, we identified five strains isolated from soil and sediments able to degrade kraft lignin, aromatic dyes and lignin derivatives. Using 16S rRNA gene sequencing, the isolates were identified as Serratia sp. JHT01, Serratia liquefacien PT01, Pseudomonas chlororaphis PT02, Stenotrophomonas maltoph...

journal_title：Letters in applied microbiology

authors： Tian JH,Pourcher AM,Peu P

更新日期：2016-07-01 00:00:00

abstract：AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...

journal_title：Letters in applied microbiology

authors： Igarashi T,Yano Y,Yamamoto A,Sasa R,Goto N

更新日期：2001-06-01 00:00:00

abstract：AIMS:To identify LipL32 epitopes and to evaluate their capability to recognize specific antibodies using ELISA. METHODS AND RESULTS:Epitope mapping by means of a library of overlapping peptide fragments prepared by simultaneous and parallel solid phase peptide synthesis on derivatized cellulose membranes (SPOT synthes...

journal_title：Letters in applied microbiology

authors： Lottersberger J,Guerrero SA,Tonarelli GG,Frank R,Tarabla H,Vanasco NB

更新日期：2009-11-01 00:00:00