In vitro interaction of actinomycetes isolates with Aspergillus flavus: impact on aflatoxins B1 and B2 production.

abstract：:A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat...

journal_title：Letters in applied microbiology

authors： Rogemond V,Da Costa Castro JM,Delaunay T,Cazaux C,Guinet RM

更新日期：1991-09-01 00:00:00

abstract：:Four strains of Escherichia coli were tested for electroporation-induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a si...

journal_title：Letters in applied microbiology

authors： Elvin S,Bingham AH

更新日期：1991-02-01 00:00:00

abstract：AIMS:This study was conducted to clarify the taxonomic status of Bacteroides type A strains with high vitamin B(12)-producing ability that is widely distributed in the intestinal tracts of freshwater fish. METHODS AND RESULTS:Seventeen strains of Bacteroides type A isolated from five fish species were all rod-shaped a...

journal_title：Letters in applied microbiology

authors： Tsuchiya C,Sakata T,Sugita H

更新日期：2008-01-01 00:00:00

abstract：AIMS:To apply scanning electron microscopy, image analysis and a fluorescent viability stain to assess lethal and sublethal injury in food-borne bacteria exposed to pulsed-plasma gas discharges (PPGD). METHODS AND RESULTS:The fluorescent redox probe 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) was used for enumerati...

journal_title：Letters in applied microbiology

authors： Rowan NJ,Espie S,Harrower J,Farrell H,Marsili L,Anderson JG,MacGregor SJ

更新日期：2008-01-01 00:00:00

abstract：AIMS:Real-time reverse transcription-polymerase chain reaction (RT-PCR) assay based on a TaqMan-minor groove binder (MGB) probe was developed for the rapid detection of avian influenza virus subtype H5. METHODS AND RESULTS:Conserved regions in the haemagglutinin genes of avian influenza viruses subtype H5 served as ta...

journal_title：Letters in applied microbiology

authors： Lu YY,Yan JY,Feng Y,Xu CP,Shi W,Mao HY

更新日期：2008-01-01 00:00:00

abstract：:A method for the diagnosis of mycobacterial infections by PCR amplification followed by selective restriction enzyme digestion of the PCR product was developed. The amplified DNA sequence used in this study occurs within the gene encoding for the mycobacterial 65 kDa heat shock protein (Hance et al. 1989), which is fo...

journal_title：Letters in applied microbiology

authors： Rodrigo G,Källenius G,Hoffmann E,Svenson SB

更新日期：1992-08-01 00:00:00

abstract：AIMS:The aim of this study was to investigate the role of divercin V41 in inhibition and prevention of Listeria monocytogenes. METHODS AND RESULTS:Carnobacterium divergens V41 deficient in bacteriocin production was isolated and characterized by enzyme-liked immunosorbent assay, multiplex polymerase chain reaction and...

journal_title：Letters in applied microbiology

authors： Richard C,Brillet A,Pilet MF,Prévost H,Drider D

更新日期：2003-01-01 00:00:00

abstract：AIMS:To immobilize Methylobacterium sp. NP3 and Acinetobacter sp. PK1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol. METHODS AND RESULTS:The phenol degradation activity of suspended and immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 bacteria wa...

journal_title：Letters in applied microbiology

authors： Khongkhaem P,Intasiri A,Luepromchai E

更新日期：2011-05-01 00:00:00

abstract：AIMS:Evaluation of the activity profiles of standard prokaryotic translation inhibitors with different physicochemical properties under whole-cell and cell-free conditions. METHODS AND RESULTS:The minimal inhibitory concentration values (cell-free/whole-cell microg ml(-1)) for three aminoglycosides (neomycin, 0.01/6.9...

journal_title：Letters in applied microbiology

authors： Weidlich M,Klammt C,Bernhard F,Karas M,Stein T

更新日期：2008-02-01 00:00:00

abstract：AIMS:To develop a colorimetric colony-screening assay to facilitate the isolation of micro-organisms capable of defluorination. METHODS AND RESULTS:A metal-dye chelate, zirconium-xylenol orange was used to detect fluoride ions released from a fluorinated substrate through microbial metabolism. Depolymerised zirconium ...

journal_title：Letters in applied microbiology

authors： Davis CK,Denman SE,Sly LI,McSweeney CS

更新日期：2011-10-01 00:00:00

abstract：:The effect of surfactant type and concentration on a bland soap formulation's ability to remove bacteria from hands remains largely unstudied. Several combinations of surfactants and water were combined to test bacterial removal efficacy using a hand-washing device (two pieces of pig skin and a mechanical motor) to si...

journal_title：Letters in applied microbiology

authors： Jensen DA,Rogers MA,Schaffner DW

更新日期：2017-10-01 00:00:00

abstract：AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designin...

journal_title：Letters in applied microbiology

authors： Mukhopadhyay HK,Amsaveni S,Matta SL,Antony PX,Thanislass J,Pillai RM

更新日期：2012-09-01 00:00:00

abstract：AIMS:The present work aimed to design an optimized medium to yield a higher production of glycosides by Pseudoalteromonas issachenkonii KMM 3549(T). METHODS AND RESULTS:Higher levels of fucoidan hydrolase, alginase, laminaranase and b-N-acetylglucosaminidase production were obtained with peptone concentrations ranging...

journal_title：Letters in applied microbiology

authors： Alexeeva YV,Ivanova EP,Bakunina IY,Zvaygintseva TN,Mikhailov VV

更新日期：2002-01-01 00:00:00

abstract：:The ability of chemotherapeutic agents to affect the growth of common bacterial pathogens and the relationship between the effects of chemotherapeutics and antimicrobials is largely unknown. The purpose of this study was to describe the susceptibility of canine bacterial isolates to chemotherapeutic agents and to comp...

journal_title：Letters in applied microbiology

authors： Campbell O,Gagnon J,Rubin JE

更新日期：2019-11-01 00:00:00

abstract：:A study was made of the incidence of Campylobacter upsaliensis among dogs and cats suffering from acute or chronic diarrhoea; 225 dogs and cats were examined and 51 strains were identified, 16 (7%) of which were Camp. upsaliensis. When rectal swabs were taken from a control group of 126 dogs and cats without clinical ...

journal_title：Letters in applied microbiology

authors： Steinhauserova I,Fojtikova K,Klimes J

更新日期：2000-09-01 00:00:00

abstract：:The type strain Bacillus thuringiensis var. bolivia (serotype H63), isolated from the Bolivian high valleys, has been characterized at different levels. Its parasporal crystal has an unusual shape and it is composed of a protein of 155 kDa which shows two bands of 75 and 80 kDa after activation. Analysis by PCR shows ...

journal_title：Letters in applied microbiology

authors： Ferrandis MD,Andrew R,Porcar M,Iriarte J,Cosmao-Dumanoir V,Lecadet MM,Caballero P,Ferré J

更新日期：1999-06-01 00:00:00

abstract：AIM:To evaluate the presence of thermotolerant Campylobacter spp. in stray cats in southern Italy. METHODS AND RESULTS:One hundred and thirteen animals were trapped in two different environments (urban area, harbour area) of the city of Naples. From each cat, rectal swabs were collected. The samples were processed in ...

journal_title：Letters in applied microbiology

authors： Gargiulo A,Rinaldi L,D'Angelo L,Dipineto L,Borrelli L,Fioretti A,Menna LF

更新日期：2008-02-01 00:00:00

abstract：AIMS:From a survey of submerged culture of edible mushrooms, a high pigment-producing fungus Paecilomyces sinclairii was selected and its optimal culture conditions investigated. METHODS AND RESULTS:The optimal culture conditions for pigment production were as follows: inoculum age, 3 d; temperature, 25 degrees C; ini...

journal_title：Letters in applied microbiology

authors： Cho YJ,Park JP,Hwang HJ,Kim SW,Choi JW,Yun JW

更新日期：2002-01-01 00:00:00

abstract：:From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown or...

journal_title：Letters in applied microbiology

authors： Ahrens P,Friis NF

更新日期：1991-06-01 00:00:00

abstract：:While the beta-glucuronidase activity of intact cells of Clostridium perfringens was higher in 0.95% sodium chloride (NaCl) than that in 0, 0.1 or 0.5%, that of Escherichia coli was higher in 0.1% NaCl than that in 0, 0.5 or 0.95% NaCl in 0.1 mol l-1 KH2PO4. However, the enzyme activity of both species of intact cells...

journal_title：Letters in applied microbiology

authors： Fujisawa T,Aikawa K,Takahashi T,Yamai S

更新日期：2000-09-01 00:00:00

abstract：:Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serot...

journal_title：Letters in applied microbiology

authors： Black SF,Gray DI,Fenlon DR,Kroll RG

更新日期：1995-03-01 00:00:00

abstract：UNLABELLED:Haemophilus influenzae serotype a (Hia) has become an important pathogen in the post-H. influenzae serotype b (Hib) vaccine era. Antibiotic resistance in H. influenzae is a global phenomenon, but few studies have looked at antibiotic resistance profiles with regard to serotype. Invasive Hia (n = 157), noninv...

journal_title：Letters in applied microbiology

authors： Shuel M,Whyte K,Drew T,Wylie J,Lefebvre B,Hoang L,Tsang RS

更新日期：2014-08-01 00:00:00

abstract：:Amphotericin B (AmB), a typical polyene macrolide antifungal agent, is widely used to treat systemic mycoses. In the present study, we show that the fungicidal activity of AmB was enhanced by benzyl isothiocyanate (BITC), a cruciferous plant-derived compound, in the budding yeast, Saccharomyces cerevisiae. In addition...

journal_title：Letters in applied microbiology

authors： Yamada N,Murata W,Yamaguchi Y,Fujita KI,Ogita A,Tanaka T

更新日期：2020-10-31 00:00:00

abstract：:Knowledge of the composition of the colonic microbiota is important for our understanding of how the balance of these microbes is influenced by diet and the environment, and which bacterial groups are important in maintaining gut health or promoting disease. Molecular methodologies have advanced our understanding of t...

journal_title：Letters in applied microbiology

authors： Duncan SH,Louis P,Flint HJ

更新日期：2007-04-01 00:00:00

abstract：:Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world. Coffee leaf blight, caused by Phomopsis heveicola, is one of the most important fungal diseases dangerous to coffee crops in China. This study aimed to develop a PCR-...

journal_title：Letters in applied microbiology

authors： Lu Y,Wu WH,He CP,Liang YQ,Huang X,Zheng JL,Xi JG,Tang SB,Yi KX

更新日期：2020-09-26 00:00:00

abstract：UNLABELLED:We investigated the role of the Bradyrhizobium japonicum lpcC gene, encoding a mannosyl transferase, involved in the lipopolysaccharide (LPS) biosynthesis. The inactivation of the lpcC gene considerably altered the LPS structure and the cell surface properties. LPS analysis showed that the lpcC mutant JS715 ...

journal_title：Letters in applied microbiology

authors： Lee HI,In YH,Jeong SY,Jeon JM,Noh JG,So JS,Chang WS

更新日期：2014-07-01 00:00:00

abstract：:Iron availability plays an important role in the virulence of micro-organisms, which develops different systems for iron acquisition. The expression of genes involved in iron uptake systems is usually regulated by Fur, a transcriptional regulator. Plesiomonas shigelloides is a Gram-negative food- and water-borne enter...

journal_title：Letters in applied microbiology

authors： Rodríguez-Rodríguez S,Santos JA

更新日期：2018-04-01 00:00:00

abstract：AIMS:  The primary goal of this study was to characterize the existence of a functional c-di-GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. METHODS AND RESULTS:  A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the ...

journal_title：Letters in applied microbiology

authors： Ruiz LM,Castro M,Barriga A,Jerez CA,Guiliani N

更新日期：2012-02-01 00:00:00

abstract：AIMS:The use of randomly amplified polymorphic DNA (RAPD)-PCR fingerprinting and plasmid profiles to determine at the strain level, the similarity of Lactococcus lactis isolates obtained during sampling of traditional cheeses and to verify its correspondence to the selected phenotypic characteristics. METHODS AND RESU...

journal_title：Letters in applied microbiology

authors： de la Plaza M,Rodríguez A,Fernández de Palencia P,Martínez-Cuesta MC,Peláez C,Requena T

更新日期：2006-12-01 00:00:00

abstract：:For pyruvate-producing strains, intracellular reduced nicotinamide adenine dinucleotide (NADH) accumulation is the main reason for the glycolysis inhibition. Comparing with glucose, using sodium gluconate as carbon source brought a decrease in NADH production and an increase in pyruvate production in engineered strain...

journal_title：Letters in applied microbiology

authors： Yang M,Mu T,Zhong W,Olajuyin AM,Xing J

更新日期：2017-08-01 00:00:00