Abstract:
AIMS:To compare the effects of five yeast probiotic formulations on viability, revival and washout kinetic in the digestive tract of mice, and the protection against an experimental infection with Salmonella enterica serovar Typhimurium. METHODS AND RESULTS:The number of viable cells in five commercial probiotic products codified as A, B, C and D (Saccharomyces boulardii- lyophilized) and E (Saccharomyces cerevisiae- aqueous suspension) was determined, as well as revival and washout kinetic in mouse intestine. Protective capacity was evaluated by survival rate and histopathology of liver and intestine of mice treated with each product and then challenged with Salm. Typhimurium. CONCLUSIONS:Product A contained the highest number of viable cells and, fed to mice, gave the highest counts of viable yeasts and the longest persistence in faeces. Probably as a consequence, the highest survival and protection of intestinal and hepatic tissues were observed when product A was used for mouse treatment. Product E showed low counts in the formulation and was not recovered from mouse intestine. SIGNIFICANCE AND IMPACT OF THE STUDY:Formulation (lyophilization or aqueous suspension) is an important factor for revival and survival of a probiotic product in vivo and consequently for its protective properties.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Martins FS,Veloso LC,Arantes RM,Nicoli JRdoi
10.1111/j.1472-765X.2009.02732.xsubject
Has Abstractpub_date
2009-12-01 00:00:00pages
738-44issue
6eissn
0266-8254issn
1472-765Xpii
LAM2732journal_volume
49pub_type
杂志文章abstract:AIMS:Strains of Clostridium butyricum have been increasingly used as probiotics for both animals and humans. The aim of this study was to develop a growth medium for cultivating C. butyricum ZJUCB using a statistical methodology. METHODS AND RESULTS:Response surface methodology (RSM) was used to evaluate the effects o...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01595.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:Saccharomyces cerevisiae was used for studying the physiological effects of hydrostatic pressure. METHODS AND RESULTS:The effects of hydrostatic pressure on the ultrastructure of wild-type and trehalose-6-phosphate synthase (tps1) mutant cells were investigated by transmission electron microscopy. Pressure induce...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00853.x
更新日期:2001-01-01 00:00:00
abstract:AIMS: Lesions of DNA are removed by nucleotide excision repair (NER) process in the living systems. NER process-related host factors are believed to aid recovery steps during viral integration. Here, we report identification and characterization of a DNA repair molecule Rad23 from kuruma shrimp Marsupenaeus japonicus. ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03073.x
更新日期:2011-07-01 00:00:00
abstract:UNLABELLED:Catfishes of the genus Panaque are known for their ability to feed on wood and hence to process cellulose fibres in their digestive systems. The paper industry uses cellulose fibres and thus has an interest in exploiting this property biomimetically: it could be employed as a pretreatment to lessen the energ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12061
更新日期:2013-06-01 00:00:00
abstract::Listonella (Vibrio) anguillarum, an important fish pathogen, is divided into 10 serotypes according to O-antigens present on the outer membrane. However, the biochemical and immunological properties of porin proteins have not been reported. In this study, the antigenicity and N-terminal amino acid sequence of the 35 k...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb00195.x
更新日期:1996-11-01 00:00:00
abstract::The cell surface hydrophobicity of micro-organisms is a characteristic that has been associated with the colonization of mammalian epithelia and with their capacity to induce diseases. Melatonin is a hormone produced by the pineal gland that affects the immune response mechanism. This study investigated, as an express...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00813.x
更新日期:2000-10-01 00:00:00
abstract::Environmental air sampling was evaluated as a method to detect the presence of M. bovis in the vicinity of infected badgers and their setts. Airborne particles were collected on gelatine filters using a commercially available air sampling instrument and tested for the presence of M. bovis using bacteriological culture...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12055
更新日期:2013-05-01 00:00:00
abstract::In a crossover study, volunteers consumed a diet supplemented with either wheat or rye bread. Levels of faecal mucus were determined and the adhesion of five selected probiotics to immobilized intestinal mucus, was assessed. No significant difference in the concentration of faecal mucus or adhesion of the probiotics t...
journal_title:Letters in applied microbiology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1472-765x.2000.00758.x
更新日期:2000-07-01 00:00:00
abstract::The effect of liquid mechanical vibration on the adhesion of Saccharomyces cerevisiae cells to the internal glass surface of a pipette was studied using a 25 Hz vibration source. The maximum vibration amplitude was 1.06 mm (peak to peak) along the pipette direction. Relative movements between the pipette and yeast sus...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb00022.x
更新日期:1996-07-01 00:00:00
abstract::A total of 87 isolates of Yersinia enterocolitica were examined with randomly amplified polymorphic DNA (RAPD) by use of three different primers. Based on the RAPD profiles, the strains could be divided into three major groups: (1) the pathogenic American serotypes, O:8, O:13ab, O:20 and O:21; (2) the pathogenic Europ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1994.tb00475.x
更新日期:1994-11-01 00:00:00
abstract:AIMS:Oenococcus oeni is a lactic acid bacterium used in wine fermentation. Two open reading frames (orfB and orfC) were identified in the upstream region of the hsp18 gene, encoding the small heat-shock protein Lo18. Expression of these genes in conditions of acid stress was studied in Escherichia coli. METHODS AND RE...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00960.x
更新日期:2001-08-01 00:00:00
abstract::Understanding mechanisms of the novel SARS-CoV2 infection and progression can provide potential novel targets for prevention and/or treatment. This could be achieved via the inhibition of viral entry and/or replication, or by suppression of the immunologic response that is provoked by the infection (known as the cytok...
journal_title:Letters in applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/lam.13334
更新日期:2020-09-01 00:00:00
abstract::Leuconostoc strains were characterized according to their antibiotic susceptibilities, carbohydrate fermentation profiles, sucrase activity patterns and plasmid content. All the strains tested were resistant to the antibiotics sulphathiazole, trimethoprim and vancomycin, and could be separated into two groups based on...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00891.x
更新日期:2001-03-01 00:00:00
abstract:AIMS:To investigate the spatial organization of endogenous and exogenously applied Lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts. METHODS AND RESULTS:Samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a Lactob...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02994.x
更新日期:2011-03-01 00:00:00
abstract:AIMS:The purpose of this study was to develop a loop-mediated isothermal amplification (LAMP) method for the rapid, sensitive and simple detection of Vibrio alginolyticus in mariculture fish. METHODS AND RESULTS:LAMP primers were designed by targeting the gyrB gene. With Bst DNA polymerase, the target DNA can be clear...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02823.x
更新日期:2010-05-01 00:00:00
abstract::The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00714.x
更新日期:2000-04-01 00:00:00
abstract:AIMS:To provide evidence that DNA-PCR diagnostics of oral pathogens based on standard sample collection by paper point insertion from the depth of the periodontal pocket can be replaced by a novel non-invasive collection method based on swab technique from the gingiva. METHODS AND RESULTS:In this study we compared the...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01269.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:Aim of the study was to develop a medium for optimal heparinase production with a strain of Aspergillus flavus (MTCC-8654) by using a multidimensional statistical approach. METHODS AND RESULTS:Statistical optimization of intracellular heparinase production by A. flavus, a new isolate, was investigated. Plackett-B...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02640.x
更新日期:2009-08-01 00:00:00
abstract:AIMS:To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS). METHODS AND RESULTS:CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time interv...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2005.01696.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To improve the production of sweet-tasting protein brazzein in Lactococcus lactis using controlled fermentation conditions. METHODS AND RESULTS:The nisin-controlled expression system was used for brazzein expression. The concentration of nisin for induction and the optical density (OD) at induction were therefore...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02297.x
更新日期:2008-02-01 00:00:00
abstract::Coliphages and enteroviruses were monitored over 12 months in sewage and air adjacent to an activated sludge plant. Both showed temporal variation but the mean count of phages in enterovirus-positive samples was not significantly different from that in enterovirus-negative samples. Hence coliphages are not necessarily...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1995.tb01042.x
更新日期:1995-09-01 00:00:00
abstract:AIMS:Bioprocess oxidative stress caused by many reactive oxygen species (ROS) can lead to largely irreversible perturbation of yeast bioprocesses. These include the production of proteins derived from recombinant DNA yeast technology (aerobically grown Saccharomyces cerevisiae). These proteins include rennin, amylogluc...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01624.x
更新日期:2005-01-01 00:00:00
abstract:AIMS:To test the efficacy of Lactobacillus johnsonii FI9785 in reducing the colonization and shedding of Salmonella enterica serotype Enteritidis, Escherichia coli O78:K80 and Clostridium perfringens in poultry. METHODS AND RESULTS:Specific pathogen-free chicks (1 day old) were dosed with a single oral inoculum of 1x1...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2004.01474.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS:Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cult...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01036.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To determine the production of reuterin by lactobacilli isolates from pig faeces and to evaluate their potential as probiotic bacteria. METHODS AND RESULTS:Twenty-eight of 165 lactobacilli isolates produced reuterin in the presence of glycerol. Six isolates yielding high levels of reuterin with respect to type st...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01390.x
更新日期:2003-01-01 00:00:00
abstract:AIMS:To find a supplemental ingredient that can be added to routinely used growth media to increase conidial production and decrease aflatoxin biosynthesis in small sclerotial (S strain) isolates of Aspergillus flavus. METHODS AND RESULTS:Molasses was added to three commonly used culture media: coconut agar (CAM), pot...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.02056.x
更新日期:2007-02-01 00:00:00
abstract:AIM:To determine if the outer membrane (OM) cytochromes OmcA and OmcB of the metal-reducing bacterium Shewanella oneidensis MR-1 have distinct or overlapping roles in the reduction of insoluble manganese(IV) oxide. METHODS AND RESULTS:The gene replacement mutant (OMCA1) which lacks OmcA was partially deficient in Mn(I...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01338.x
更新日期:2003-01-01 00:00:00
abstract::A study was made of the incidence of Campylobacter upsaliensis among dogs and cats suffering from acute or chronic diarrhoea; 225 dogs and cats were examined and 51 strains were identified, 16 (7%) of which were Camp. upsaliensis. When rectal swabs were taken from a control group of 126 dogs and cats without clinical ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00799.x
更新日期:2000-09-01 00:00:00
abstract:AIM:The automated TEMPO system (bioMerieux) is based on the most probable number (MPN) method for the enumeration of micro-organisms in foods. In this study, we evaluated the performance of the TEMPO system as a diagnostic tool in comparison with the standard method in processed soy products. METHODS AND RESULTS:A ver...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03143.x
更新日期:2011-11-01 00:00:00
abstract:AIMS:In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. METHODS AND RESULTS:Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.02033.x
更新日期:2007-01-01 00:00:00