Abstract:
AIMS:Aim of the study was to develop a medium for optimal heparinase production with a strain of Aspergillus flavus (MTCC-8654) by using a multidimensional statistical approach. METHODS AND RESULTS:Statistical optimization of intracellular heparinase production by A. flavus, a new isolate, was investigated. Plackett-Burman design was used to evaluate the affect of medium constituents on heparinase yield. The experimental results showed that the production of heparinase was dependent upon heparin, the inducer; chitin, structurally similar to heparin and NH(4)NO(3,) the nitrogen source. A central composite design was applied to derive a statistical model for optimizing the composition of the fermentation medium for the production of heparinase enzyme. The optimum fermentation medium consisted of (g l(-1)) Mannitol, 8.0; NH(4)NO(3), 2.5; K(2)HPO(4), 2.5; Na(2)HPO(4), 2.5; MgSO(4).7H(2)O, 0.5; Chitin, 17.1; Heparin, 0.6; trace salt solution (NaMoO(4).2H(2)O, CoCl(2).6H(2)O, CuSO(4).5H(2)O, FeSO(4).7H(2)O, CaCl(2)), 10(-4) mol l(-1). CONCLUSIONS:A 2.37-fold increase in heparinase production was achieved in economic and effective manner by the application of statistical designs in medium optimization. SIGNIFICANCE AND IMPACT OF THE STUDY:Heparinase production was doubled by statistical optimization in a cost-effective manner. This heparinase can find application in pharmaceutical industry and for the generation of low-molecular-weight heparins, active as antithrombotic and antitumour agents.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Banga J,Tripathi CKdoi
10.1111/j.1472-765X.2009.02640.xsubject
Has Abstractpub_date
2009-08-01 00:00:00pages
204-9issue
2eissn
0266-8254issn
1472-765Xpii
LAM2640journal_volume
49pub_type
杂志文章abstract:AIMS:To investigate the genetics of dimethoate degradation in Pseudomonas aeruginosa MCMB-427. METHODS AND RESULTS:Pseudomonas aeruginosa MCMB-427 demonstrated the ability to degrade dimethoate, a synthetic organophosphate insecticide. Total DNA preparation of MCMB-427 revealed the presence of a 6.6 kbp plasmid (desig...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00995.x
更新日期:2001-10-01 00:00:00
abstract:AIMS:To compare the effects of five yeast probiotic formulations on viability, revival and washout kinetic in the digestive tract of mice, and the protection against an experimental infection with Salmonella enterica serovar Typhimurium. METHODS AND RESULTS:The number of viable cells in five commercial probiotic produ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02732.x
更新日期:2009-12-01 00:00:00
abstract:AIMS:To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination. METHODS AND RESULTS:Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences wer...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01948.x
更新日期:2006-09-01 00:00:00
abstract:AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02786.x
更新日期:2010-03-01 00:00:00
abstract::Limited data are available on the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in the UK. We tested 124 raw meat samples for MRSA including pork (n = 63), chicken (n = 50) and turkey (n = 11) collected from retail outlets in North West England between March and July 2015. MR...
journal_title:Letters in applied microbiology
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doi:10.1111/lam.12709
更新日期:2017-03-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2005.01715.x
更新日期:2005-01-01 00:00:00
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journal_title:Letters in applied microbiology
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doi:10.1111/j.1472-765X.2011.03152.x
更新日期:2011-12-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01098.x
更新日期:2002-01-01 00:00:00
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journal_title:Letters in applied microbiology
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doi:10.1111/lam.12263
更新日期:2014-08-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13425
更新日期:2020-10-31 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2003.01464.x
更新日期:2004-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2017-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12571
更新日期:2016-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00733.x
更新日期:2000-05-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb01110.x
更新日期:1996-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02655.x
更新日期:2009-08-01 00:00:00
abstract:UNLABELLED:During a screening for potential plant disease control actinomycetes, a total of 133 strains were isolated from Saharan soil samples of seven Algerian regions by dilution technique on chitin-vitamins agar medium. Screening for antagonistic properties using streak assay method showed that 25% of isolates demo...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.2012.03312.x
更新日期:2012-12-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01081.x
更新日期:2002-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01911.x
更新日期:2006-07-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02132.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12432
更新日期:2015-08-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02567.x
更新日期:2009-05-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01573.x
更新日期:2004-01-01 00:00:00
abstract:AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in dochi (fermented black beans), a traditional fermented food in Taiwan. METHODS AND RESULTS:A total of 30 samples were collected from three different dochi producers and analysed after different periods of storage. Fifty-two cultures of LAB were i...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01922.x
更新日期:2006-08-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00891.x
更新日期:2001-03-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2015-12-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03111.x
更新日期:2011-09-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.2000.00793.x
更新日期:2000-08-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00598.x
更新日期:1999-08-01 00:00:00