Response surface methodology for optimization of medium components in submerged culture of Aspergillus flavus for enhanced heparinase production.

Abstract:

AIMS:Aim of the study was to develop a medium for optimal heparinase production with a strain of Aspergillus flavus (MTCC-8654) by using a multidimensional statistical approach. METHODS AND RESULTS:Statistical optimization of intracellular heparinase production by A. flavus, a new isolate, was investigated. Plackett-Burman design was used to evaluate the affect of medium constituents on heparinase yield. The experimental results showed that the production of heparinase was dependent upon heparin, the inducer; chitin, structurally similar to heparin and NH(4)NO(3,) the nitrogen source. A central composite design was applied to derive a statistical model for optimizing the composition of the fermentation medium for the production of heparinase enzyme. The optimum fermentation medium consisted of (g l(-1)) Mannitol, 8.0; NH(4)NO(3), 2.5; K(2)HPO(4), 2.5; Na(2)HPO(4), 2.5; MgSO(4).7H(2)O, 0.5; Chitin, 17.1; Heparin, 0.6; trace salt solution (NaMoO(4).2H(2)O, CoCl(2).6H(2)O, CuSO(4).5H(2)O, FeSO(4).7H(2)O, CaCl(2)), 10(-4) mol l(-1). CONCLUSIONS:A 2.37-fold increase in heparinase production was achieved in economic and effective manner by the application of statistical designs in medium optimization. SIGNIFICANCE AND IMPACT OF THE STUDY:Heparinase production was doubled by statistical optimization in a cost-effective manner. This heparinase can find application in pharmaceutical industry and for the generation of low-molecular-weight heparins, active as antithrombotic and antitumour agents.

journal_name

Lett Appl Microbiol

authors

Banga J,Tripathi CK

doi

10.1111/j.1472-765X.2009.02640.x

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

204-9

issue

2

eissn

0266-8254

issn

1472-765X

pii

LAM2640

journal_volume

49

pub_type

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