Isolation and characterization of lactic acid bacteria from dochi (fermented black beans), a traditional fermented food in Taiwan.

abstract：UNLABELLED:Four strains were isolated from a spoiled whiting (Merlangius merlangus). All of them were able to grow aerobically from 4 to 30°C and also able to develop anaerobically in the presence of trimethylamine N-Oxide (TMAO) at 25°C. Biochemical characterization did not allow identification of the strains species ...

journal_title：Letters in applied microbiology

authors： Dehaut A,Midelet-Bourdin G,Brisabois A,Duflos G

更新日期：2014-11-01 00:00:00

abstract：AIMS:The use of randomly amplified polymorphic DNA (RAPD)-PCR fingerprinting and plasmid profiles to determine at the strain level, the similarity of Lactococcus lactis isolates obtained during sampling of traditional cheeses and to verify its correspondence to the selected phenotypic characteristics. METHODS AND RESU...

journal_title：Letters in applied microbiology

authors： de la Plaza M,Rodríguez A,Fernández de Palencia P,Martínez-Cuesta MC,Peláez C,Requena T

更新日期：2006-12-01 00:00:00

abstract：:Colistin-resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high-resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known geno...

journal_title：Letters in applied microbiology

authors： Tahmasebi H,Dehbashi S,Arabestani MR

更新日期：2020-04-01 00:00:00

abstract：UNLABELLED:Staphylococcus aureus LZ-01 was isolated from the Yellow River upstream from Lanzhou which can resist and reduce chromium (VI) to chromium (III). In this study, strain LZ-01's uranium (VI) resistance and adsorption abilities were investigated. Our results showed that it can resist 2 mmol l(-1) U(VI) and adso...

journal_title：Letters in applied microbiology

authors： Zou L,Chen Z,Zhang X,Liu P,Li X

更新日期：2014-11-01 00:00:00

abstract：:Heat stable antifungal factor (HSAF) is considered to be a potential biological pesticide due to its broad antifungal activity and novel mode of action. However, few studies have reported on HSAF production during fermentation. Thus, this work was executed to optimize the medium composition to maximize HSAF production...

journal_title：Letters in applied microbiology

authors： Tang B,Zhao YC,Shi XM,Xu HY,Zhao YY,Dai CC,Liu FQ

更新日期：2018-05-01 00:00:00

abstract：AIMS:The objective of this study is to actively express a novel fibrinolytic enzyme, subtilisin DFE (douchi fibrinolytic enzyme), in Escherichia coli. METHODS AND RESULTS:The DNA fragments encoding pro-subtilisin DFE was amplified and cloned into the vector pET32a to obtain N-terminal Trx fusion expression plasmid. Th...

journal_title：Letters in applied microbiology

authors： Zhang RH,Xiao L,Peng Y,Wang HY,Bai F,Zhang YZ

更新日期：2005-01-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：AIM:To study the effect of biosurfactant on aqueous phase solubility and biodegradation of chlorpyrifos. METHODS AND RESULTS:A Pseudomonas sp. (ChlD), isolated from agricultural soil by enrichment culture technique in the presence of chlorpyrifos, was capable of producing biosurfactant (rhamnolipids) and degrading chl...

journal_title：Letters in applied microbiology

authors： Singh PB,Sharma S,Saini HS,Chadha BS

更新日期：2009-09-01 00:00:00

abstract：:Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses in dairy industry worldwide. In addition, milk and milk-products contaminated by Staph. aureus can cause harmful human diseases. The aim of this study was to characterize Staph. aureus strains isolated from dairy farms in...

journal_title：Letters in applied microbiology

authors： Ben Said M,Abbassi MS,Bianchini V,Sghaier S,Cremonesi P,Romanò A,Gualdi V,Hassen A,Luini MV

更新日期：2016-12-01 00:00:00

abstract：AIMS:A rapid and simple method for enumerating uninjured and sublethally injured bacterial cells, the twofold dilution method (2FD), was developed and evaluated. METHODS AND RESULTS:Following twofold serial dilution of samples in a 96 well microtiter plate, double strength selective broth or nonselective broth was add...

journal_title：Letters in applied microbiology

authors： Kang DH,Siragusa GR

更新日期：2001-09-01 00:00:00

abstract：UNLABELLED:Chrysanthemum plants are subject to serious virus diseases, so detection and identification of virus pathogens is important to prevent the virus spread. A reliable one-step multiplex RT-PCR was developed to simultaneously detect two viruses and two viriods: chrysanthemum virus B, tomato Aspermy virus, chrysa...

journal_title：Letters in applied microbiology

authors： Song A,You Y,Chen F,Li P,Jiang J,Chen S

更新日期：2013-01-01 00:00:00

abstract：AIMS: Lesions of DNA are removed by nucleotide excision repair (NER) process in the living systems. NER process-related host factors are believed to aid recovery steps during viral integration. Here, we report identification and characterization of a DNA repair molecule Rad23 from kuruma shrimp Marsupenaeus japonicus. ...

journal_title：Letters in applied microbiology

authors： Sudhakaran R,Okugawa S,Mekata T,Inada M,Yoshimine M,Nishi J,Ozono C,Kono T,Sakai M,Itami T

更新日期：2011-07-01 00:00:00

abstract：:A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference...

journal_title：Letters in applied microbiology

authors： Chang JH,Roh JY,Je YH,Park HW,Jin BR,Woo SD,Kang SK

更新日期：1998-05-01 00:00:00

abstract：:Alzheimer's disease (AD) is the most common form of dementia and one of the major causes of disability and dependency in older people. Accumulating evidences link gut microbiota with different diseases and its relationship with neurodegenerative diseases is becoming most intriguing. This study was aimed to compare the...

journal_title：Letters in applied microbiology

authors： Bäuerl C,Collado MC,Diaz Cuevas A,Viña J,Pérez Martínez G

更新日期：2018-06-01 00:00:00

abstract：:A rapid and simple method (24M) using 24 well microtitre plates was developed to determine the presence of Listeria monocytogenes or Listeria spp. in food samples. The 24M was composed of two 24 well microtitre plates connected with a yellow tip. The 24M was evaluated with pathogen cocktails and ground beef samples an...

journal_title：Letters in applied microbiology

authors： Kang DH,Fung DY

更新日期：1999-04-01 00:00:00

abstract：AIMS:The study was carried out to evaluate the use of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) as a method for the identification of lactobacilli isolated from meat products. METHODS AND RESULTS:RAPD-PCR with primers M13 and D8635 was applied to the identification and intraspecific diffe...

journal_title：Letters in applied microbiology

authors： Andrighetto C,Zampese L,Lombardi A

更新日期：2001-07-01 00:00:00

abstract：AIMS:To determine the effect of cold storage on the survival of Erwinia amylovora. METHODS AND RESULTS:The survival of E. amylovora was assessed during storage at 2 degrees C. Populations of E. amylovora inoculated into phosphate-buffered saline remained static, whereas in nutrient media populations increased at low t...

journal_title：Letters in applied microbiology

authors： Taylor RK,Hale CN

更新日期：2003-01-01 00:00:00

abstract：AIMS:The aims of this study were to investigate in vitro the effects of Lactobacillus isolates from a chicken on adhesion of pathogenic Salmonella and Escherichia coli to chicken intestinal mucus obtained from different intestinal regions. METHODS AND RESULTS:Bacteria were labelled by using methyl-1,2-[(3)H]-thymidine...

journal_title：Letters in applied microbiology

authors： Ma YL,Guo T,Xu ZR,You P,Ma JF

更新日期：2006-04-01 00:00:00

abstract：:Erwinia amylovora bacteria cause fire blight disease, which affects apple and pear production worldwide. The Erw. amylovora pyrC gene encodes a predicted dihydroorotase enzyme involved in pyrimidine biosynthesis. Here, we discovered that the Erw. amylovora pyrC244::Tn5 mutant was a uracil auxotroph. Unexpectedly, the ...

journal_title：Letters in applied microbiology

authors： Ramos LS,Sinn JP,Lehman BL,Pfeufer EE,Peter KA,McNellis TW

更新日期：2015-06-01 00:00:00

abstract：AIMS:The aim of this study was to identify and determine the diversity, occurrence and distribution of fungi in water used at a haemodialysis centre. METHODS AND RESULTS:Samples in the hydraulic circuit for the distribution of the water, dialysate samples and samples of sterilization solution from dialysers were colle...

journal_title：Letters in applied microbiology

authors： Pires-Gonçalves RH,Sartori FG,Montanari LB,Zaia JE,Melhem MS,Mendes-Giannini MJ,Martins CH

更新日期：2008-05-01 00:00:00

abstract：AIMS:To develop and evaluate a real-time PCR assay incorporating an internal amplification control (IAC) suitable for the screening of Shiga toxin (Stx)-producing Escherichia coli (STEC) in foods. METHODS AND RESULTS:A competitive IAC was constructed and included in an stx-specific real-time PCR assay. Coupled to 18-h...

journal_title：Letters in applied microbiology

authors： Auvray F,Lecureuil C,Dilasser F,Taché J,Derzelle S

更新日期：2009-05-01 00:00:00

abstract：:The Andrias davidianus has been known as a traditional Chinese medicine for a long time. Its blood is considered as a waste or by-product of the meat production industry. Although there are reports on isolation of the antimicrobial peptides from different resources, there are no reports of their isolation from A. davi...

journal_title：Letters in applied microbiology

authors： Pei J,Feng Z,Ren T,Sun H,Han H,Jin W,Dang J,Tao Y

更新日期：2018-01-01 00:00:00

abstract：AIMS:The objective was to demonstrate the size of silver particles produced by the filamentous fungus Phoma sp.3.2883 via adsorption and accumulation, and to confirm that this silver was in a reduced state. METHODS AND RESULTS:Mycelium was freeze-dried and then shake-cultured in a silver nitrate solution. It was found...

journal_title：Letters in applied microbiology

authors： Chen JC,Lin ZH,Ma XX

更新日期：2003-01-01 00:00:00

abstract：:PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni a...

journal_title：Letters in applied microbiology

authors： Rasmussen HN,Olsen JE,Jørgensen K,Rasmussen OF

更新日期：1996-11-01 00:00:00

abstract：:Listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and raw & undercooked meats. Stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface mater...

journal_title：Letters in applied microbiology

authors： Redfern J,Verran J

更新日期：2017-04-01 00:00:00

abstract：:Vacuum-packaged poultry cooked sausages were pressure-treated at 500 MPa by combinations of time (5-45 min) and temperature (2-80 degrees C) and later stored at 6-8 degrees C for 12 we. Mesophile and psychrotrophe counts were determined 1 d, 3, 6, 9 and 12 we after treatment and compared with those of cooked sausages ...

journal_title：Letters in applied microbiology

authors： Yuste J,Mor-Mur M,Capellas M,Pla R

更新日期：1999-10-01 00:00:00

abstract：AIMS:To identify the abattoir source(s) of psychrophilic clostridia causing 'blown pack' spoilage of vacuum-packed chilled meats. METHODS AND RESULTS:Molecular procedures were used to detect the presence of specific 16S rRNA gene fragments of blown pack-causing clostridia in samples collected from a commercial abattoi...

journal_title：Letters in applied microbiology

authors： Boerema JA,Broda DM,Bell RG

更新日期：2003-01-01 00:00:00

abstract：AIMS:To isolate gamma-hexachlorocyclohexane (gamma-HCH)-degrading bacteria from a single field and to examine their genetic diversity. METHODS AND RESULTS:Gamma-HCH-degrading bacteria were screened from a long-term experimental field in which gamma-HCH has been continuously applied to, and a gamma-HCH-degrading sphing...

journal_title：Letters in applied microbiology

authors： Yamamoto S,Otsuka S,Murakami Y,Nishiyama M,Senoo K

更新日期：2009-10-01 00:00:00

abstract：:The present study was aimed at determining antimicrobial susceptibility by a CLSI standard microdilution testing protocol and detecting the resistance genes of motile Aeromonas species isolated from cultured fish. The importance of the minimum inhibitory concentrations was assessed based on statistically determined ep...

journal_title：Letters in applied microbiology

authors： Duman M,Saticioglu IB,Altun S

更新日期：2020-11-01 00:00:00

abstract：:A lead-resistant bacteria isolated from soil contaminated with car battery waste were identified as Providencia alcalifaciens based on biochemical characteristics, FAME profile and 16S rRNA sequencing and designated as strain 2EA. It resists lead nitrate up to 0·0014 mol l(-1) by precipitating soluble lead as insolubl...

journal_title：Letters in applied microbiology

authors： Naik MM,Khanolkar D,Dubey SK

更新日期：2013-02-01 00:00:00