FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts.

abstract：AIMS:To evaluate a new dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) assay for detection of six sexually transmitted pathogens, including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum and Trichomonas vaginalis. METHO...

journal_title：Letters in applied microbiology

authors： Horii T,Ohtsuka H,Osaki M,Ohkuni H

更新日期：2009-07-01 00:00:00

abstract：AIMS:The effect of high organic loading rate (OLR) on the physical characteristics of aerobic granules was studied. METHODS AND RESULTS:Two column-type sequential aerobic sludge blanket reactors were fed with either glucose or acetate as the main carbon source, and the OLR was gradually raised from 6 to 9, 12 and 15 k...

journal_title：Letters in applied microbiology

authors： Moy BY,Tay JH,Toh SK,Liu Y,Tay ST

更新日期：2002-01-01 00:00:00

abstract：:The neurotoxins produced by Clostridium botulinum are amongst the most potent known to man. Toxin production is detected by a mouse bioassay, which requires several days for a result and is not acceptable for routine use unless there is a high level of suspicion. The Rapid ID32 A kit produced by bioMerieux gives an id...

journal_title：Letters in applied microbiology

authors： Brett MM

更新日期：1998-01-01 00:00:00

abstract：AIMS:The development of a simple, rapid and sensitive method for the screening of chitinase inhibitors using Ostazin Brilliant Red labelled chitin as a substrate for chitinase assay. METHODS AND RESULTS:The colloidal substrate prepared from the Ostazin Brilliant Red labelled chitin was used in the estimation of chitin...

journal_title：Letters in applied microbiology

authors： Shirazi F,Kulkarni M,Deshpande MV

更新日期：2007-06-01 00:00:00

abstract：:The present study demonstrates the siderophore production of two strains of Pseudomonas fluorescens and two of Ps. chlororaphis. The antimicrobial activities of these strains were studied against Salmonella typhimurium, Staphylococcus aureus, Fusarium culmorum, F. oxysporum and Aspergillus niger. Despite equal siderop...

journal_title：Letters in applied microbiology

authors： Laine MH,Karwoski MT,Raaska LB,Mattila-Sandholm TM

更新日期：1996-03-01 00:00:00

abstract：:Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram-positive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which re...

journal_title：Letters in applied microbiology

authors： McCormick JK,Klaenhammer TR,Stiles ME

更新日期：1999-07-01 00:00:00

abstract：:The recent rise in the awareness of the occurrence of toxic cyanobacterial blooms in aquatic environments, with associated human health problems and animal deaths, has increased the need for rapid, reliable and sensitive methods of determining cyanobacterial toxicity. A luminescent bacterial toxicity test was assessed...

journal_title：Letters in applied microbiology

authors： Lawton LA,Campbell DL,Beattie KA,Codd GA

更新日期：1990-10-01 00:00:00

abstract：:Yellow wine lees (YWL) are the main co-products in yellow wine industry with unbalanced amino acid (AA) profiles. Solid-state fermentation was employed in this study to upgrade the YWL for ruminant animals. A 3 × 3 orthogonal design was conducted to optimize the fermentation condition for optimal crude protein (CP) yi...

journal_title：Letters in applied microbiology

authors： Yao KY,Zhang TZ,Wang HF,Liu JX

更新日期：2018-12-01 00:00:00

abstract：:Patterns of long-chain faecal fatty acids were studied by gas-liquid chromatography in 55 newborn infants in a neonatal intensive care unit. Decreased fractions of fatty acid C16:1 and increased fractions of C16:0 and C17:0 were associated with the occurrence of abdominal distension. Decreased fractions of C16:1 and C...

journal_title：Letters in applied microbiology

authors： Ahtonen P,Lehtonen OP,Kero P,Eerola E

更新日期：1997-02-01 00:00:00

abstract：AIMS:Bioprocess oxidative stress caused by many reactive oxygen species (ROS) can lead to largely irreversible perturbation of yeast bioprocesses. These include the production of proteins derived from recombinant DNA yeast technology (aerobically grown Saccharomyces cerevisiae). These proteins include rennin, amylogluc...

journal_title：Letters in applied microbiology

authors： Wiseman A

更新日期：2005-01-01 00:00:00

abstract：:In order to select a suitable indicator for monitoring the pathogen regrowth potential of composted biosolids, the growth kinetics of selected bacteria were investigated. Growth parameters of six serovars of Salmonella and three strains of Escherichia coli in sterilized compost were compared. Seeded Salmonella and E. ...

journal_title：Letters in applied microbiology

authors： Sidhu J,Gibbs RA,Ho GE,Unkovich I

更新日期：1999-11-01 00:00:00

abstract：AIM:To compare the incidence of antimicrobial resistance among Salmonella serotypes isolated in a pig slaughterhouse in Zaragoza (Spain) during 1993 and 2001. METHODS AND RESULTS:A total of 168 isolates representing 10 serotypes were examined by disc diffusion method using 17 antibiotics. Data showed that the majority...

journal_title：Letters in applied microbiology

authors： Agustín AI,Carramiñana JJ,Rota C,Herrera A

更新日期：2005-01-01 00:00:00

abstract：:Random amplification of polymorphic DNA (RAPD) is proving to be a useful technique in studying the epidemiology of micro-organisms. The technique can be troublesome and time consuming to establish due to the essentially empirical approach to optimization. By standardization of certain parameters and use of a commercia...

journal_title：Letters in applied microbiology

authors： Hilton AC,Banks JG,Penn CW

更新日期：1997-04-01 00:00:00

abstract：:Protoplasts of Streptomyces flavopersicus with the highest regeneration frequency were isolated from late log phase mycelium grown in a two-stage culture system with 2% glycine in the medium. Of the 11 regeneration media tested, R9 was selected as the most efficient with 29 degrees C as the best temperature. ...

journal_title：Letters in applied microbiology

authors： Lyutzkanova D,Nikolova B,Stoilova-Discheva M,Todorov T

更新日期：1993-04-01 00:00:00

abstract：AIMS:This study evaluated the inactivation of virulent Yersinia pestis dried on polymers, steel, and glass surfaces using vapour-phase hydrogen peroxide. METHODS AND RESULTS:A suspension of Y. pestis CO92 (1.70 x 10(8) CFU) was dried on 10 different types of test surfaces and exposed to vapour-phase hydrogen peroxide ...

journal_title：Letters in applied microbiology

authors： Rogers JV,Richter WR,Shaw MQ,Choi YW

更新日期：2008-10-01 00:00:00

abstract：AIMS:2-Keto-4-methylthiobutyric acid (KMBA) is an established intermediate in microbial biosynthesis of ethylene from methionine. This study demonstrates the kinetics and effects of trace elements and electron complexes on substrate (KMBA)-derived C2H4 biosynthesis in soil. METHODS AND RESULTS:We have previously repor...

journal_title：Letters in applied microbiology

authors： Arshad M,Nazli ZH,Khalid A,Zahir ZA

更新日期：2004-01-01 00:00:00

abstract：:Recent studies have shown that Salmonella shedding status affects sows' microbiota during gestation and that these modifications are reflected in the faecal microbiota of their piglets at weaning. The aims of this study were: (a) to evaluate the persistence, up to the fattening period, of the previously measured link ...

journal_title：Letters in applied microbiology

authors： Larivière-Gauthier G,Thibodeau A,Yergeau É,Fravalo P

更新日期：2021-02-01 00:00:00

abstract：AIMS:Tuberculous pleurisy is an important cause of pleural effusions in areas with a high incidence of tuberculosis. In this study, we developed an IS1081-based LAMP for the detection of Mycobacterium tuberculosis complex and investigated its usefulness in the diagnosis of tuberculous pleurisy. METHODS AND RESULTS:Inv...

journal_title：Letters in applied microbiology

authors： Yang B,Wang X,Li H,Li G,Cao Z,Cheng X

更新日期：2011-11-01 00:00:00

abstract：AIMS:To study the viability of a culture of the rumen protozoon Entodinium caudatum after a cryopreservation procedure by a fluorescence microscopy staining method. METHODS AND RESULTS:Fluorescence method is based on the different colour of cells depending on their membrane integrity. When the temperature effect was s...

journal_title：Letters in applied microbiology

authors： de la Fuente G,Cebrián JA,Fondevila M

更新日期：2004-01-01 00:00:00

abstract：:To enhance the overall expression level of lipase isozymes which catalyse the same reaction in Pichia pastoris through co-expression of isozymes from different sources; several types of co-expression ways were constructed to determine the co-expression efficiencies of lipase isozymes in P. pastoris. The results showed...

journal_title：Letters in applied microbiology

authors： Cai H,Zhang T,Zhao M,Mao J,Cai C,Feng F

更新日期：2017-10-01 00:00:00

abstract：:Seventeen aerobic bacterial strains known to degrade one or more aromatic compounds were tested for their ability to utilize benzene, seven different methylated benzenes or other aromatic compounds as their sole source of carbon. The results are discussed with respect to the possibility of finding strains which degrad...

journal_title：Letters in applied microbiology

authors： Lang E

更新日期：1996-10-01 00:00:00

abstract：:SDS-PAGE fingerprinting of cell-wall proteins extracted from 119 strains belonging to different species of lactic acid bacteria have been compared. The method of extraction and electrophoretic separation utilized in this work was found to be a reliable and rapid way for characterizing thermophilic lactobacilli species...

journal_title：Letters in applied microbiology

authors： Gatti M,Fornasari E,Neviani E

更新日期：1997-11-01 00:00:00

abstract：:Increasingly, molecular methods have become important in identification and confirmation of bacteria at the species level. Rapid molecular methods provide sensitivity and specificity while reducing cost and resources. The primary goal of this study was to develop a real-time PCR assay for identification of Escherichia...

journal_title：Letters in applied microbiology

authors： Tillman GE,Simmons M,Wasilenko JL,Narang N,Cray WC Jr,Bodeis-Jones S,Martin G,Gaines S,Seal BS

更新日期：2015-02-01 00:00:00

abstract：:The effect of surfactant type and concentration on a bland soap formulation's ability to remove bacteria from hands remains largely unstudied. Several combinations of surfactants and water were combined to test bacterial removal efficacy using a hand-washing device (two pieces of pig skin and a mechanical motor) to si...

journal_title：Letters in applied microbiology

authors： Jensen DA,Rogers MA,Schaffner DW

更新日期：2017-10-01 00:00:00

abstract：AIMS:The present work aimed to design an optimized medium to yield a higher production of glycosides by Pseudoalteromonas issachenkonii KMM 3549(T). METHODS AND RESULTS:Higher levels of fucoidan hydrolase, alginase, laminaranase and b-N-acetylglucosaminidase production were obtained with peptone concentrations ranging...

journal_title：Letters in applied microbiology

authors： Alexeeva YV,Ivanova EP,Bakunina IY,Zvaygintseva TN,Mikhailov VV

更新日期：2002-01-01 00:00:00

abstract：:Phorate [O,O-diethyl-S-(ethylthio)methyl phosphoradiothioate] degrading bacteria were isolated from agricultural soil and characterized based on their morphological and biochemical characteristics. The selected isolates PS-1, PS-2 and PS-3 were presumptively identified as Rhizobium, Pseudomonas and Proteous species, r...

journal_title：Letters in applied microbiology

authors： Bano N,Musarrat J

更新日期：2003-01-01 00:00:00

abstract：:The growth of the oxidatively imperfect yeast Candida maltosa Komagata, Nakase et Katsuya was studied experimentally and modelled mathematically in relation to sodium benzoate and lactic acid concentrations at different temperatures. Application of gamma models for the growth rate resulted in determination of cardinal...

journal_title：Letters in applied microbiology

authors： Valík Ľ,Ačai P,Liptáková D

更新日期：2017-11-01 00:00:00

abstract：AIMS:The present study describes the implementation of real-time PCR to tetrathionate broth enrichment step of Salmonella detection in poultry. METHODS AND RESULTS:Real-time PCR with Salmonella invA-specific primers and a standard bacteriological method was applied to detect Salmonella in tetrathionate enrichment cult...

journal_title：Letters in applied microbiology

authors： Eyigor A,Carli KT,Unal CB

更新日期：2002-01-01 00:00:00

abstract：:The use of buoyant density centrifugation (BDC) to prepare samples for PCR analysis of food pathogens is described. Blue cheese and milk homogenates were inoculated with Shigella flexneri and layered on top of Percoll media. After BDC, the food homogenates remained in the upper part of the centrifuge tube, separated f...

journal_title：Letters in applied microbiology

authors： Lindqvist R,Norling B,Lambertz ST

更新日期：1997-04-01 00:00:00

abstract：UNLABELLED:Bioconversion of biomass, particularly crop wastes, into biofuels is being developed as an alternative approach in meeting the high energy demand. In this study, a thermophilic bacterial strain BY-3 that exhibits cellulolytic potential was isolated from faecal samples of Tibetan pigs; this strain was identif...

journal_title：Letters in applied microbiology

authors： Meng F,Ma L,Ji S,Yang W,Cao B

更新日期：2014-09-01 00:00:00