Evaluation of the use of the bioMerieux Rapid ID32 A for the identification of Clostridium botulinum.

abstract：:Colistin-resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high-resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known geno...

journal_title：Letters in applied microbiology

authors： Tahmasebi H,Dehbashi S,Arabestani MR

更新日期：2020-04-01 00:00:00

abstract：:The use of buoyant density centrifugation (BDC) to prepare samples for PCR analysis of food pathogens is described. Blue cheese and milk homogenates were inoculated with Shigella flexneri and layered on top of Percoll media. After BDC, the food homogenates remained in the upper part of the centrifuge tube, separated f...

journal_title：Letters in applied microbiology

authors： Lindqvist R,Norling B,Lambertz ST

更新日期：1997-04-01 00:00:00

abstract：:A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for separating homologous amplicons from the respective uidA region differing in one to seven single base substitutions. Bo...

journal_title：Letters in applied microbiology

authors： Farnleitner AH,Kreuzinger N,Kavka GG,Grillenberger S,Rath J,Mach RL

更新日期：2000-06-01 00:00:00

abstract：AIMS:To examine the efficacy of liposome oral administration to induce systemic and mucosal immune responses against verotoxin-producing Escherichia coli (VTEC) and the effect of the induced antibodies on the binding of the bacteria to Caco-2 cells. METHODS AND RESULTS:Mice were immunized orally with VTEC antigen and ...

journal_title：Letters in applied microbiology

authors： Tana,Watarai S,Isogai E,Oguma K

更新日期：2003-01-01 00:00:00

abstract：AIMS:In this study, we compared different methods of colistin susceptibility testing, disc diffusion, agar dilution and Etest using a set of Enterobacteriaceae isolates that included colistin-resistant strains. METHODS AND RESULTS:Susceptibility of 200 clinical isolates of Enterobacteriaceae to colistin was tested to ...

journal_title：Letters in applied microbiology

authors： Maalej SM,Meziou MR,Rhimi FM,Hammami A

更新日期：2011-11-01 00:00:00

abstract：AIMS:The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). METHODS AND RESULTS:Of the polymers tested, PE and PE + PEO did not exhibit an...

journal_title：Letters in applied microbiology

authors： Cutter CN,Willett JL,Siragusa GR

更新日期：2001-10-01 00:00:00

abstract：AIMS:To evaluate mycobiota and aflatoxins B(1) (AFB(1)), B(2) (AFB(2)), G(1) (AFG(1)), G(2) (AFG(2)) and fumonisin B(1) (FB(1)) contamination in different malted barley types and brands and brewer's grain collected from a major Argentinean brewery. METHODS AND RESULTS:Total fungal counts were performed using the plate...

journal_title：Letters in applied microbiology

authors： Gonzalez Pereyra ML,Rosa CA,Dalcero AM,Cavaglieri LR

更新日期：2011-12-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：AIMS:To identify and compare microbiota in Chinese liquor Daqu, which were produced in the different regions using different production process. METHODS AND RESULTS:The DNA exacted from Daqu samples was used as a template for PCR with universal primers of 16S rRNA, 26S rRNA and 18S rRNA, respectively. The amplicons we...

journal_title：Letters in applied microbiology

authors： Wang HY,Gao YB,Fan QW,Xu Y

更新日期：2011-08-01 00:00:00

abstract：AIMS:Enzyme kinetics of purified laccases from six different Pleurotus ostreatus strains were determined in the oxidation of syringaldazine, guaiacol and ABTS. METHODS AND RESULTS:Significant differences in the kinetic constants were found. Catalytic activity (kcat) ranged from 19 to 941 U mg(-1) for syringaldazine, f...

journal_title：Letters in applied microbiology

authors： Tinoco R,Pickard MA,Vazquez-Duhalt R

更新日期：2001-05-01 00:00:00

abstract：:A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. je...

journal_title：Letters in applied microbiology

authors： Mazurier S,van de Giessen A,Heuvelman K,Wernars K

更新日期：1992-06-01 00:00:00

abstract：:Limited data are available on the prevalence of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in the UK. We tested 124 raw meat samples for MRSA including pork (n = 63), chicken (n = 50) and turkey (n = 11) collected from retail outlets in North West England between March and July 2015. MR...

journal_title：Letters in applied microbiology

authors： Fox A,Pichon B,Wilkinson H,Doumith M,Hill RL,McLauchlin J,Kearns AM

更新日期：2017-03-01 00:00:00

abstract：AIMS:Species-specific PCR was applied to identify Lactobacillus brevis and the sensitivity and the specificity of the protocol were determined. METHODS AND RESULTS:Strains of Lact. brevis obtained from foods, particularly dairy products, and various strain collections, were identified by PCR using primers which amplif...

journal_title：Letters in applied microbiology

authors： Guarneri T,Rossetti L,Giraffa G

更新日期：2001-11-01 00:00:00

abstract：AIMS:To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS). METHODS AND RESULTS:CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time interv...

journal_title：Letters in applied microbiology

authors： Pellé E,Dousset X,Prévost H,Drider D

更新日期：2005-01-01 00:00:00

abstract：:The contribution of trehalose and hsp104 to barotolerance in Saccharomyces cerevisiae has been investigated. Mutant strains, which lacked the ability to accumulate trehalose and/or hsp104, were examined for barotolerance and thermotolerance. All the mutants showed lower barotolerance and thermotolerance than their con...

journal_title：Letters in applied microbiology

authors： Iwahashi H,Obuchi K,Fujii S,Komatsu Y

更新日期：1997-07-01 00:00:00

abstract：AIMS:In this study, we report a new, simple methodology for the monitoring of Cryptosporidium oocysts and Giardia cysts in drinking water samples, ranging from 10- to 1000-l, which combines a new ARAD microfibre filtration of the (oo)cysts from drinking water and loop-mediated isothermal amplification (LAMP) of a human...

journal_title：Letters in applied microbiology

authors： Plutzer J,Törökné A,Karanis P

更新日期：2010-01-01 00:00:00

abstract：AIMS:To immobilize Methylobacterium sp. NP3 and Acinetobacter sp. PK1 to silica and determine the ability of the immobilized bacteria to degrade high concentrations of phenol. METHODS AND RESULTS:The phenol degradation activity of suspended and immobilized Methylobacterium sp. NP3 and Acinetobacter sp. PK1 bacteria wa...

journal_title：Letters in applied microbiology

authors： Khongkhaem P,Intasiri A,Luepromchai E

更新日期：2011-05-01 00:00:00

abstract：:Quillaja saponin (QS) was incubated at 39 degrees C in an in vitro medium containing rumen liquor from a cow fed a roughage diet. No degradation of QS was observed up to 6 h of fermentation. Incubation for 9, 12 and 24 h decreased the content of QS by 16%, 45% and 100%. The content of QS did not decrease when incubate...

journal_title：Letters in applied microbiology

authors： Makkar HP,Becker K

更新日期：1997-10-01 00:00:00

abstract：AIMS:The aim of this study was to detect and quantify DNA of the ochratoxinogenic fungus Aspergillus ochraceus in green coffee and to compare the results with the ochratoxin A content of naturally contaminated samples. METHODS AND RESULTS:A DNA extraction protocol based on a combination of ultrasonification and a comm...

journal_title：Letters in applied microbiology

authors： Schmidt H,Bannier M,Vogel RF,Niessen L

更新日期：2004-01-01 00:00:00

abstract：AIMS:Immunologically based assays for the detection of staphylococcal enterotoxins are numerous. These techniques include radio immunosorbent assays and enzyme-linked immunosorbent assays (ELISA), some of which are available as commercial kits. The purpose of this study was to compare the performances of three commerci...

journal_title：Letters in applied microbiology

authors： Vernozy-Rozand C,Mazuy-Cruchaudet C,Bavai C,Richard Y

更新日期：2004-01-01 00:00:00

abstract：:A monoclonal antibody (mAb) has been produced to aflatoxin B1 (AF B1) after successful immunization of mice and fusion of sensitized spleen cells with myeloma cancer cells. The mice were immunized with AF B1-oxime-protein conjugate. Positive mAbs were screened using an indirect ELISA specific for AF B1. The selected m...

journal_title：Letters in applied microbiology

authors： Candlish AA,Smith JE,Stimson WH

更新日期：1990-04-01 00:00:00

abstract：:Washed Escherichia coli ATCC11775 cells were killed by (-)-epigallocatechin (EGC) in the presence of a non-lethal concentration of Cu2+ (1 mumol l-1) without additional H2O2, but not by (-)-epicatechin (EC). EGC alone (< 0.1 mmol l-1) did not reduce the viability of the cells. The survival curve obtained in the presen...

journal_title：Letters in applied microbiology

authors： Kimura T,Hoshino N,Yamaji A,Hayakawa F,Ando T

更新日期：1998-12-01 00:00:00

abstract：:Three white rot fungi were compared for their ability to attack polychlorinated biphenyl (PCB) congeners in the presence and absence of the non-ionic Triton X-100 or the anionic Dowfax 8390 surfactants at half their critical micelle concentrations. Neither surfactant affected PCB biodegradation monitored by gas chroma...

journal_title：Letters in applied microbiology

authors： Beaudette LA,Ward OP,Pickard MA,Fedorak PM

更新日期：2000-02-01 00:00:00

abstract：AIMS:This study was performed to investigate the microbiological contamination of digestate product (DP) obtained from the anaerobic co-digestion of bovine manure and agricultural by-products. METHODS AND RESULTS:Microbiological analyses were performed on bovine manure, fresh DP, liquid and solid fractions and stored ...

journal_title：Letters in applied microbiology

authors： Bonetta S,Ferretti E,Bonetta S,Fezia G,Carraro E

更新日期：2011-11-01 00:00:00

abstract：AIMS:To investigate the spatial organization of endogenous and exogenously applied Lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts. METHODS AND RESULTS:Samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a Lactob...

journal_title：Letters in applied microbiology

authors： Lebeer S,Verhoeven TL,Claes IJ,De Hertogh G,Vermeire S,Buyse J,Van Immerseel F,Vanderleyden J,De Keersmaecker SC

更新日期：2011-03-01 00:00:00

abstract：AIMS:To find a supplemental ingredient that can be added to routinely used growth media to increase conidial production and decrease aflatoxin biosynthesis in small sclerotial (S strain) isolates of Aspergillus flavus. METHODS AND RESULTS:Molasses was added to three commonly used culture media: coconut agar (CAM), pot...

journal_title：Letters in applied microbiology

authors： Chang PK,Hua SS

更新日期：2007-02-01 00:00:00

abstract：:The development of various types of virus-like particles (VLPs) has accelerated over the past two decades as the importance of VLPs for generating next-generation vaccines has been appreciated. Yeast has advantages such as scalable fermentation, low risk of contamination by adventitious agents, low production costs an...

journal_title：Letters in applied microbiology

authors： Kim HJ,Kim HJ

更新日期：2017-02-01 00:00:00

abstract：:Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

journal_title：Letters in applied microbiology

authors： Wang SQ,Wang T,Liu JF,Deng L,Wang F

更新日期：2018-11-01 00:00:00

abstract：AIMS:The current study examined the antimicrobial susceptibility of 86 Listeria spp. isolated from processed bison carcasses. MATERIALS AND METHODS:Susceptibility to 25 antimicrobial agents was determined using E-test and National Antimicrobial Resistance Monitoring System (NARMS) panels. Most Listeria isolates (88-98...

journal_title：Letters in applied microbiology

authors： Li Q,Sherwood JS,Logue CM

更新日期：2007-01-01 00:00:00

abstract：AIMS:This work was carried out to study the acid production by Lactococcus lactis subsp. lactis strains isolated from goat's milk and goat cheese (Valdeteja variety) in order to select a suitable starter culture for industrial goat cheese manufacturing. METHODS AND RESULTS:The titrable acidity of 45 Lactococcus lactis...

journal_title：Letters in applied microbiology

authors： Alonso-Calleja C,Carballo J,Capita R,Bernardo A,García-López ML

更新日期：2002-01-01 00:00:00