RAPD analysis of Campylobacter isolates: DNA fingerprinting without the need to purify DNA.

Abstract:

:A method was developed to obtain reproducible DNA fingerprints from Campylobacter by PCR-based amplification, without the need to isolate total DNA. Randomly amplified polymorphic DNA (RAPD) profiles were generated with three randomly designed 10-mers, using each separately as an amplification primer. A range of C. jejuni serotypes could be typed by RAPD analysis. Depending on the primer, the analysis of RAPD profiles resulted in different levels of discrimination between the strains. Clear correlations were observed between results of RAPD analysis and serotyping. Two of the primers tested generated RAPD profiles which allowed discrimination of strains within given Penner and Lior serotypes.

journal_name

Lett Appl Microbiol

authors

Mazurier S,van de Giessen A,Heuvelman K,Wernars K

doi

10.1111/j.1472-765x.1992.tb00700.x

subject

Has Abstract

pub_date

1992-06-01 00:00:00

pages

260-2

issue

6

eissn

0266-8254

issn

1472-765X

journal_volume

14

pub_type

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