Abstract:
AIMS:To evaluate mycobiota and aflatoxins B(1) (AFB(1)), B(2) (AFB(2)), G(1) (AFG(1)), G(2) (AFG(2)) and fumonisin B(1) (FB(1)) contamination in different malted barley types and brands and brewer's grain collected from a major Argentinean brewery. METHODS AND RESULTS:Total fungal counts were performed using the plate count method. Aflatoxin B(1), AFB(2), AFG(1), AFG(2) and Zearalenone (ZEA) analyses were performed by thin-layer chromatography (TLC). Fumonisin B(1) was determined by HPLC. Eighty-three percentage of the malted barley (100% M1, 50% M2 and 100% M3) and 61% of brewer's grain samples had a count >1 × 10(4) CFU g(-1). Yeasts were isolated from all malt and brewer's grain samples. Genera containing some of the most important mycotoxin producer species--Fusarium ssp., Aspergillus ssp., Penicillium ssp. and Alternaria ssp.--were isolated from the analysed samples, along with other environmental saprophytic fungi such as Geotrichum ssp., Mucorales and Cladosporium ssp. All samples were contaminated with 104-145 μg kg(-1) FB(1). Eighteen per cent of brewer's grain samples were contaminated with 19-44.52 μg kg(-1) AFB(1). Aflatoxin B(2), AFG(1), AFG(2) and ZEA were not detected in any of the analysed samples. CONCLUSIONS:Fungal and mycotoxin contamination in malt and brewer's grain is an actual risk for animal and human health. SIGNIFICANCE AND IMPACT OF THE STUDY:This study may be useful for assessing the risk of mycotoxins in Argentinean beers and especially in animal feeds.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Gonzalez Pereyra ML,Rosa CA,Dalcero AM,Cavaglieri LRdoi
10.1111/j.1472-765X.2011.03157.xsubject
Has Abstractpub_date
2011-12-01 00:00:00pages
649-55issue
6eissn
0266-8254issn
1472-765Xjournal_volume
53pub_type
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