Detection and quantification of Aspergillus ochraceus in green coffee by PCR.

abstract：:Accumulation of proline and glycine betaine occurs via three transport systems in Staphylococcus aureus (Pourkomailian and Booth 1992, 1994). A 35 kDa membrane protein was identified to be missing from a mutant, BP108, which lacks activity of the low affinity osmolyte transport system. The loss in activity of the low ...

journal_title：Letters in applied microbiology

authors： Pourkomaillan B

更新日期：1998-02-01 00:00:00

abstract：AIM:The automated TEMPO system (bioMerieux) is based on the most probable number (MPN) method for the enumeration of micro-organisms in foods. In this study, we evaluated the performance of the TEMPO system as a diagnostic tool in comparison with the standard method in processed soy products. METHODS AND RESULTS:A ver...

journal_title：Letters in applied microbiology

authors： Katase M,Tsumura K

更新日期：2011-11-01 00:00:00

abstract：AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...

journal_title：Letters in applied microbiology

authors： Igarashi T,Yano Y,Yamamoto A,Sasa R,Goto N

更新日期：2001-06-01 00:00:00

abstract：AIMS:Several cases of campylobacteriosis reported worldwide seemingly conflict with the strict growth requirements and sensitivity to environmental stress of Campylobacter jejuni. In this study, the need for a micro-aerobic environment [dissolved oxygen tension (DOT): 0.1-90%; 100% air saturation)] and the adaptive res...

journal_title：Letters in applied microbiology

authors： Verhoeff-Bakkenes L,Arends AP,Snoep JL,Zwietering MH,de Jonge R

更新日期：2008-03-01 00:00:00

abstract：AIMS:To optimize the production of linolenic acid by disrupted mycelia of Mortierella isabellina. METHODS AND RESULTS:Effects of incubation conditions such as incubation time, pH of reaction mixture, concentration of Mg2+ or malate and incubation temperature on production of linolenic acid were studied. The production...

journal_title：Letters in applied microbiology

authors： Xian M,Nie J,Meng Q,Liu J,Zhou C,Kang Y,Zhen K

更新日期：2003-01-01 00:00:00

abstract：:Parabens have been widely used as antimicrobial preservatives in cosmetics, pharmaceuticals, foods and beverages. Commonly, methyl-, ethyl-, propyl- and butylparaben are used independently or in combination to maintain the quality of industrial products, and they are considered to have low toxicity. However, recent ev...

journal_title：Letters in applied microbiology

authors： Murata W,Yamaguchi Y,Fujita KI,Yamauchi K,Tanaka T,Ogita A

更新日期：2019-12-01 00:00:00

abstract：AIMS:To identify LipL32 epitopes and to evaluate their capability to recognize specific antibodies using ELISA. METHODS AND RESULTS:Epitope mapping by means of a library of overlapping peptide fragments prepared by simultaneous and parallel solid phase peptide synthesis on derivatized cellulose membranes (SPOT synthes...

journal_title：Letters in applied microbiology

authors： Lottersberger J,Guerrero SA,Tonarelli GG,Frank R,Tarabla H,Vanasco NB

更新日期：2009-11-01 00:00:00

abstract：AIMS:To propose a universal workflow of sample preparation method for the identification of highly pathogenic bacteria by MALDI-TOF MS. METHODS AND RESULTS:Fifteen bacterial species, including highly virulent Gram-positive (Bacillus anthracis and Clostridium botulinum) and Gram-negative bacteria (Brucella melitensis, ...

journal_title：Letters in applied microbiology

authors： Drevinek M,Dresler J,Klimentova J,Pisa L,Hubalek M

更新日期：2012-07-01 00:00:00

abstract：AIM:The mechanisms of adaptation of Clostridium perfringens to high temperatures are not well understood. In this work, the involvement of extracellular compounds in protection to heat was determined. METHODS AND RESULTS:Cells were grown in fluid thioglycollate medium or chicken broth. When mid-log phase was reached, ...

journal_title：Letters in applied microbiology

authors： Heredia N,Ybarra P,Hernández C,García S

更新日期：2009-01-01 00:00:00

abstract：AIMS:To investigate fusion expression between Bacillus thuringiensis crystal protein and a foreign protein, the expression of a fusion protein comprised of Cry1Ac, and enhanced green fluorescent protein (EGFP) in B. thuringiensis Cry(-)B strain was examined. METHODS AND RESULTS:The N-terminal fusion expression of EGFP...

journal_title：Letters in applied microbiology

authors： Roh JY,Li MS,Chang JH,Choi JY,Shim HJ,Shin SC,Boo KS,Je YH

更新日期：2004-01-01 00:00:00

abstract：:The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 m...

journal_title：Letters in applied microbiology

authors： Shin K,Yamauchi K,Teraguchi S,Hayasawa H,Tomita M,Otsuka Y,Yamazaki S

更新日期：1998-06-01 00:00:00

abstract：AIMS:To evaluate the accuracy of pyrosequencing for the description of Phytophthora communities in terms of taxa identification and risk of assignment for false Molecular Operational Taxonomic Units (MOTUs). METHODS AND RESULTS:Pyrosequencing of Internal Transcribed Spacer 1 (ITS1) amplicons was used to describe the s...

journal_title：Letters in applied microbiology

authors： Vettraino AM,Bonants P,Tomassini A,Bruni N,Vannini A

更新日期：2012-11-01 00:00:00

abstract：AIMS:To examine the ability of Agrobacterium to attach to Metarhizium anisopliae var. acridum strain CG423 under co-cultivation and to develop an Agrobacterium-mediated method of gene delivery into strain CG423, a promising agent for biological control of grasshoppers. METHODS AND RESULTS:The co-cultivation of Agrobac...

journal_title：Letters in applied microbiology

authors： Duarte RT,Staats CC,Fungaro MH,Schrank A,Vainsten MH,Furlaneto-Maia L,Nakamura CV,de Souza W,Furlaneto MC

更新日期：2007-03-01 00:00:00

abstract：AIMS:The development of a simple, rapid and sensitive method for the screening of chitinase inhibitors using Ostazin Brilliant Red labelled chitin as a substrate for chitinase assay. METHODS AND RESULTS:The colloidal substrate prepared from the Ostazin Brilliant Red labelled chitin was used in the estimation of chitin...

journal_title：Letters in applied microbiology

authors： Shirazi F,Kulkarni M,Deshpande MV

更新日期：2007-06-01 00:00:00

abstract：AIMS:The objective of the present study was to determine the optimal culture conditions for exo-biopolymer production by Cordyceps militaris in shake flask culture. METHODS AND RESULTS:The optimal temperature and initial pH for both mycelial growth and exo-biopolymer production by Cordyceps militaris in shake flask cu...

journal_title：Letters in applied microbiology

authors： Park JP,Kim SW,Hwang HJ,Yun JW

更新日期：2001-07-01 00:00:00

abstract：:Entamoeba histolytica and Entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. The former is a pathogen, whereas the latter is a nonpathogenic commensal. Using a model system of in vitro cocultures and PCR detection of the Entamoeba species, we found that the nonpathogenic spec...

journal_title：Letters in applied microbiology

authors： Pysova I,Tumova P,Tolarova V,Nohynkova E

更新日期：2009-04-01 00:00:00

abstract：:This study was undertaken to detect the occurrence of beta-lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n = 92) and backyard (n = 110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87...

journal_title：Letters in applied microbiology

authors： Banerjee A,Bardhan R,Chowdhury M,Joardar SN,Isore DP,Batabyal K,Dey S,Sar TK,Bandyopadhyay S,Dutta TK,Samanta I

更新日期：2019-08-01 00:00:00

abstract：:Nisin, a bacteriocin produced by some strains of Lactococcus lactis, acts against foodborne pathogen Listeria monocytogenes. A single exposure of cells to nisin can generate nisin-resistant (Nisr) mutants, which may compromise the use of nisin in the food industry. The objective of this research was to compare the hea...

journal_title：Letters in applied microbiology

authors： Modi KD,Chikindas ML,Montville TJ

更新日期：2000-03-01 00:00:00

abstract：:Three groups of substances were identified which inhibit the bactericidal activity of bacitracin. Beside two divalent metals, Mg2+ and Ca2+, and a metabolite with chelating properties, citrate, the most pronounced effect was observed with pyrophosphate. Metals probably prevent access of bacitracin to the lipid carrier...

journal_title：Letters in applied microbiology

authors： Podlesek Z,Comino A

更新日期：1994-08-01 00:00:00

abstract：:Condensed tannins (CTs), which extracted from yew leaves, tilia flower and black locust leaves, were examined for their antimicrobial in vitro activity against avian pathogenic Escherichia coli (APEC). Past research demonstrated that CTs which contain procyanidins and prodelphinidins that could inhibit the growth of a...

journal_title：Letters in applied microbiology

authors： Dakheel MM,Alkandari FAH,Mueller-Harvey I,Woodward MJ,Rymer C

更新日期：2020-03-01 00:00:00

abstract：AIM:To evaluate the presence of thermotolerant Campylobacter spp. in stray cats in southern Italy. METHODS AND RESULTS:One hundred and thirteen animals were trapped in two different environments (urban area, harbour area) of the city of Naples. From each cat, rectal swabs were collected. The samples were processed in ...

journal_title：Letters in applied microbiology

authors： Gargiulo A,Rinaldi L,D'Angelo L,Dipineto L,Borrelli L,Fioretti A,Menna LF

更新日期：2008-02-01 00:00:00

abstract：:The present study demonstrates the siderophore production of two strains of Pseudomonas fluorescens and two of Ps. chlororaphis. The antimicrobial activities of these strains were studied against Salmonella typhimurium, Staphylococcus aureus, Fusarium culmorum, F. oxysporum and Aspergillus niger. Despite equal siderop...

journal_title：Letters in applied microbiology

authors： Laine MH,Karwoski MT,Raaska LB,Mattila-Sandholm TM

更新日期：1996-03-01 00:00:00

abstract：AIMS:To examine changes in membrane fatty acid profile attributed to the physiological adaptation of Lactobacillus helveticus during vacuum drying. METHODS AND RESULTS:The viability and membrane integrity of the cells after vacuum drying were measured by plate counts and DNA fluorescence dyes. The physiological adapta...

journal_title：Letters in applied microbiology

authors： Santivarangkna C,Kulozik U,Kienberger H,Foerst P

更新日期：2009-10-01 00:00:00

abstract：AIMS:To evaluate a new dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) assay for detection of six sexually transmitted pathogens, including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum and Trichomonas vaginalis. METHO...

journal_title：Letters in applied microbiology

authors： Horii T,Ohtsuka H,Osaki M,Ohkuni H

更新日期：2009-07-01 00:00:00

abstract：AIMS:To investigate if one hypothetical protein from Chlamydophila pneumoniae AR39 exerts UbiG-like function by complementary experiments. METHODS AND RESULTS:Proteins UbiG have a signature S-adenosylmethionine-binding motif compared with other methyltransferases. Probing with the conserved motif, one hypothetical pro...

journal_title：Letters in applied microbiology

authors： Liu J,Zhang X,Liu J

更新日期：2007-07-01 00:00:00

abstract：:Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...

journal_title：Letters in applied microbiology

authors： Qureshi S,Saxena HM,Imam N,Kashoo Z,Sharief Banday M,Alam A,Malik MZ,Ishrat R,Bhat B

更新日期：2018-09-01 00:00:00

abstract：:A new ultrasonic apparatus operating at a frequency of 40 kHz was developed to dislodge biofilms from food processing equipment in order to assess the effectiveness of cleaning protocols. Sonication conditions to remove biofilms and quantification by ATP-bioluminescence are described. An industrial meat process was de...

journal_title：Letters in applied microbiology

authors： Oulahal-Lagsir N,Martial-Gros A,Boistier E,Blum LJ,Bonneau M

更新日期：2000-01-01 00:00:00

abstract：AIMS:In this study, we report a new, simple methodology for the monitoring of Cryptosporidium oocysts and Giardia cysts in drinking water samples, ranging from 10- to 1000-l, which combines a new ARAD microfibre filtration of the (oo)cysts from drinking water and loop-mediated isothermal amplification (LAMP) of a human...

journal_title：Letters in applied microbiology

authors： Plutzer J,Törökné A,Karanis P

更新日期：2010-01-01 00:00:00

abstract：AIMS:The current study examined the antimicrobial susceptibility of 86 Listeria spp. isolated from processed bison carcasses. MATERIALS AND METHODS:Susceptibility to 25 antimicrobial agents was determined using E-test and National Antimicrobial Resistance Monitoring System (NARMS) panels. Most Listeria isolates (88-98...

journal_title：Letters in applied microbiology

authors： Li Q,Sherwood JS,Logue CM

更新日期：2007-01-01 00:00:00

abstract：:Five phage types have been identified in 38 strains of Salmonella enteritidis isolated in Turkey in the 20-month period June 1992-January 1994. Strains belonging to phage type 4 predominated. Within phage type 4, plasmid profile typing has proved a useful method of strain discrimination and has confirmed the identity ...

journal_title：Letters in applied microbiology

authors： Erdem B,Threlfall EJ,Schofield SL,Ward LR,Rowe B

更新日期：1994-10-01 00:00:00