Abstract:
:To enhance the overall expression level of lipase isozymes which catalyse the same reaction in Pichia pastoris through co-expression of isozymes from different sources; several types of co-expression ways were constructed to determine the co-expression efficiencies of lipase isozymes in P. pastoris. The results showed that the Kex2-mediated co-expression of lipase isozymes could express Rhizomucor miehei lipase (RML) and Thermomyces lanuginosus lipase (TLL) simultaneously, and GS-RMk-kTL displayed an average lipase activity of 306·91 U ml-1 , higher than GS-RML and GS-kTL (2·89 and 300·59 U ml-1 ) expressed independently in P. pastoris, and the sum of both (303·48 U ml-1 ), implying the potential of isozyme co-expression mediated by Kex2 in increasing the overall recombinant expression, but the low recombinant expression of RML in P. pastoris weakened the overall increasing effect on lipase expression in the isozyme co-expression strains. In addition, the fusion isozymes were successfully expressed, but with low lipase activities. Furthermore, 2A peptide could successfully mediate the co-expression and secretion of lipase isozymes, but it seriously affected the expression of TLL downstream of 2A peptide. SIGNIFICANCE AND IMPACT OF THE STUDY:The low production level is one of the limitation factors for decreasing the prices of enzymes and expanding their application in industry as the biocatalysts. This research focuses on developing lipase isozyme co-expression strategies in Pichia pastoris to enhance the expression level of overall lipase isozymes which catalyse the same reaction. The Kex2-mediated co-expression strategy of lipase isozymes could potentially enhance the overall isozyme expression, and isozyme co-expression might provide a new direction for improving the recombinant isozyme expression, and decreasing the production and application prices of these mixed enzymes as biocatalysts.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Cai H,Zhang T,Zhao M,Mao J,Cai C,Feng Fdoi
10.1111/lam.12783subject
Has Abstractpub_date
2017-10-01 00:00:00pages
335-342issue
4eissn
0266-8254issn
1472-765Xjournal_volume
65pub_type
杂志文章abstract:AIMS:This study was designed to evaluate the prevalence of Clostridium difficile contamination of retail chicken. METHODS AND RESULTS:Chicken legs, thighs and wings were purchased using a standardized method from retail outlets across Ontario, Canada. Selective culture was used for qualitative and quantitative detecti...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02802.x
更新日期:2010-04-01 00:00:00
abstract::In order to evaluate possible indicators of viral aerosol contamination in sewage treatment plants, a year-long study was carried out on the relationships between the presence of cytopathogenic viruses and the counts of total bacteria, faecal streptococci and somatic coliphages in samples collected at various distance...
journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
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doi:10.1111/j.1472-765X.2004.01524.x
更新日期:2004-01-01 00:00:00
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01624.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02446.x
更新日期:2008-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:1995-04-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12391
更新日期:2015-05-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2001-09-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1365-2672.1999.00626.x
更新日期:1999-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02874.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2018-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02949.x
更新日期:2010-12-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.01014.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12117
更新日期:2013-10-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2012.03284.x
更新日期:2012-09-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb01110.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2005.01707.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03157.x
更新日期:2011-12-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01978.x
更新日期:2006-10-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2012.03244.x
更新日期:2012-06-01 00:00:00
abstract::Knowledge of the composition of the colonic microbiota is important for our understanding of how the balance of these microbes is influenced by diet and the environment, and which bacterial groups are important in maintaining gut health or promoting disease. Molecular methodologies have advanced our understanding of t...
journal_title:Letters in applied microbiology
pub_type: 杂志文章,评审
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更新日期:2007-04-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02595.x
更新日期:2009-06-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01152.x
更新日期:2002-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2004-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.01912.x
更新日期:2006-05-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12263
更新日期:2014-08-01 00:00:00
abstract::Recent studies have shown that Salmonella shedding status affects sows' microbiota during gestation and that these modifications are reflected in the faecal microbiota of their piglets at weaning. The aims of this study were: (a) to evaluate the persistence, up to the fattening period, of the previously measured link ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13406
更新日期:2021-02-01 00:00:00