A transient expression assay for the in planta efficacy screening of an antimicrobial peptide against grapevine bacterial pathogens.

abstract：:The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...

journal_title：Letters in applied microbiology

authors： García-Ortega L,Lacadena J,Lacadena V,Masip M,De Antonio C,Martínez-Ruiz A,Martínez Del Pozo A

更新日期：2000-04-01 00:00:00

abstract：UNLABELLED:Escherichia coli is part of the normal microflora of the intestines of mammals. However, among the enteric pathogens, it is one of the leading causes of intestinal diseases, especially Shiga toxigenic E. coli, which can cause diarrhoea, haemorrhagic colitis and complications like haemolytic uraemic syndrome ...

journal_title：Letters in applied microbiology

authors： Ribeiro LF,Barbosa MM,de Rezende Pinto F,Guariz CS,Maluta RP,Rossi JR,Rossi GA,Lemos MV,do Amaral LA

更新日期：2016-03-01 00:00:00

abstract：:Nanobacteria, also known as calcifying nanoparticles (CNP), are controversial infectious agents not matching the current criteria for 'living organism'. Despite the controversy of their classification, they propagate and cause cell death in vitro and are associated or found in many human diseases. Thus, more efforts s...

journal_title：Letters in applied microbiology

authors： Kajander EO

更新日期：2006-06-01 00:00:00

abstract：:Increasingly, molecular methods have become important in identification and confirmation of bacteria at the species level. Rapid molecular methods provide sensitivity and specificity while reducing cost and resources. The primary goal of this study was to develop a real-time PCR assay for identification of Escherichia...

journal_title：Letters in applied microbiology

authors： Tillman GE,Simmons M,Wasilenko JL,Narang N,Cray WC Jr,Bodeis-Jones S,Martin G,Gaines S,Seal BS

更新日期：2015-02-01 00:00:00

abstract：:Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses in dairy industry worldwide. In addition, milk and milk-products contaminated by Staph. aureus can cause harmful human diseases. The aim of this study was to characterize Staph. aureus strains isolated from dairy farms in...

journal_title：Letters in applied microbiology

authors： Ben Said M,Abbassi MS,Bianchini V,Sghaier S,Cremonesi P,Romanò A,Gualdi V,Hassen A,Luini MV

更新日期：2016-12-01 00:00:00

abstract：:Thirty-eight isolates of Hafnia alvei were characterized by biochemical profiles, ribotyping and pulsed-field gel electrophoresis (PFGE) patterns. The isolates were recovered from chub-packed (19 isolates) or retail (nine isolates) ground beef, or were obtained from culture repositories (10 isolates). Biochemical prof...

journal_title：Letters in applied microbiology

authors： Gamage SD,Luchansky JB,Ingham SC

更新日期：1998-02-01 00:00:00

abstract：AIMS: To develop a specific and highly sensitive loop-mediated isothermal amplification (LAMP) technique for the rapid detection of canine parvovirus (CPV) DNA directly in suspected faecal samples of dogs by employing a simple method of template preparation. METHODS AND RESULTS: LAMP reaction was developed by designin...

journal_title：Letters in applied microbiology

authors： Mukhopadhyay HK,Amsaveni S,Matta SL,Antony PX,Thanislass J,Pillai RM

更新日期：2012-09-01 00:00:00

abstract：:Colicin V is a small, proteinaceous bacterial toxin, produced by many strains of Escherichia coli and other members of the Enterobacteriaceae, that fits the definition of class II bacteriocins of Gram-positive bacteria. Export of colicin V is dependent on specific ABC (ATP-binding cassette) secretion proteins which re...

journal_title：Letters in applied microbiology

authors： McCormick JK,Klaenhammer TR,Stiles ME

更新日期：1999-07-01 00:00:00

abstract：:Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a worldwide concern. Cattle are their main reservoir and may contaminate watercourses through manure. We characterized a collection of 38 STEC O157:H7 strains isolated from surface water in feedlots areas (puddles inside pens formed after the rainfall or by spil...

journal_title：Letters in applied microbiology

authors： Tanaro JD,Pianciola LA,D'Astek BA,Piaggio MC,Mazzeo ML,Zolezzi G,Rivas M

更新日期：2018-06-01 00:00:00

abstract：:Nicotinic acid (NA) is ubiquitous in nature and its microbial degradation mechanisms are diverse. In this study, Pusillimonas sp. strain T2 was found to be capable of utilizing NA as sole carbon and nitrogen sources. This strain could completely degrade 300 mg l-1 NA within 3·5 h at 30°C and pH 7·0 and one of the degr...

journal_title：Letters in applied microbiology

authors： Yuan M,Zhang Y,Zhao L,Ma Y,He Q,He J,Qiu J

更新日期：2018-04-01 00:00:00

abstract：:Flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degrees C, 5 min) and nisin treatments (0.1 and 1.0 microgram ml-1) of La...

journal_title：Letters in applied microbiology

authors： Ueckert JE,Nebe von-Caron G,Bos AP,ter Steeg PF

更新日期：1997-10-01 00:00:00

abstract：UNLABELLED:Most plant species are colonized by endophytic bacteria. Despite their importance for plant health and growth, the response of these bacteria to grassland management regimes is still not understood. Hence, we investigated the bacterial community structure in three agricultural important grass species Dactyli...

journal_title：Letters in applied microbiology

authors： Wemheuer F,Wemheuer B,Kretzschmar D,Pfeiffer B,Herzog S,Daniel R,Vidal S

更新日期：2016-04-01 00:00:00

abstract：AIMS:To assess the ability of staphylococcal bacteriophage K to inhibit Staphylococcus aureus in raw milk. METHODS AND RESULTS:The ability of bacteriophage (phage) to replicate in milk is important in situations where phage might be used as a therapeutic for bovine mastitis. Phage K was able to replicate normally, lea...

journal_title：Letters in applied microbiology

authors： O'Flaherty S,Coffey A,Meaney WJ,Fitzgerald GF,Ross RP

更新日期：2005-01-01 00:00:00

abstract：:A 21-mer DNA oligonucleotide probe targeting the 23S rRNA of Mycobacterium leprae was developed and its high specificity demonstrated by dot-blot hybridization. Even under relaxed hybridization and washing conditions (20 degrees C below Tm) the probe was highly selective in that positive signals were only detected wit...

journal_title：Letters in applied microbiology

authors： Liesack W,Pitulle C,Stackebrandt E

更新日期：1990-08-01 00:00:00

abstract：:A pilot survey of the counts of total coliform bacteria, thermotolerant coliform bacteria, Escherichia coli and faecal streptococci was carried out at eight inland recreational waters at weekly intervals during July 1991. The aims were to assess the feasibility of determining candidate indicators of recreational water...

journal_title：Letters in applied microbiology

authors：

更新日期：1995-10-01 00:00:00

abstract：AIMS:To provide comparative genome sequence data for two related model strains of Staphylococcus aureus (SH1000 and 8325-4) that are used extensively in laboratory research. METHODS AND RESULTS:Comparative genome sequencing was used to identify genetic differences between Staph. aureus SH1000 and the fully genome-sequ...

journal_title：Letters in applied microbiology

authors： O'Neill AJ

更新日期：2010-09-01 00:00:00

abstract：AIMS:The objective of this study was to determine the antifungal activity of flurbiprofen against dermatophytes like Trichophyton, Microsporum and Epidermophyton species. METHODS AND RESULTS:Susceptibility tests were performed against dermatophytes like Trichophyton, Microsporum and Epidermophyton species by the micro...

journal_title：Letters in applied microbiology

authors： Chowdhury B,Adak M,Bose SK

更新日期：2003-01-01 00:00:00

abstract：:Recent studies have shown that Salmonella shedding status affects sows' microbiota during gestation and that these modifications are reflected in the faecal microbiota of their piglets at weaning. The aims of this study were: (a) to evaluate the persistence, up to the fattening period, of the previously measured link ...

journal_title：Letters in applied microbiology

authors： Larivière-Gauthier G,Thibodeau A,Yergeau É,Fravalo P

更新日期：2021-02-01 00:00:00

abstract：AIMS:To develop a PCR-based method for quantitative detection of Fusarium asiaticum (Fa) and Fusarium graminearum (Fg) in wheat seeds. METHODS AND RESULTS:Based on the sequences of the cyp51A gene, two primer pairs FaF + FaR and FgF + FgR were developed for the species-specific detection of Fa and Fg, respectively. To...

journal_title：Letters in applied microbiology

authors： Yin Y,Liu X,Ma Z

更新日期：2009-06-01 00:00:00

abstract：AIMS:To determine whether nonspore-forming biothreat agents can survive and persist in potable water that does not contain a disinfectant. METHODS AND RESULTS: Autoclaved, de-chlorinated Atlanta municipal water was inoculated with eight isolates of bacterial biothreat agents (10⁶ CFU ml⁻¹). The inoculated water sample...

journal_title：Letters in applied microbiology

authors： Gilbert SE,Rose LJ

更新日期：2012-09-01 00:00:00

abstract：AIMS:The study was carried out to evaluate the use of randomly amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) as a method for the identification of lactobacilli isolated from meat products. METHODS AND RESULTS:RAPD-PCR with primers M13 and D8635 was applied to the identification and intraspecific diffe...

journal_title：Letters in applied microbiology

authors： Andrighetto C,Zampese L,Lombardi A

更新日期：2001-07-01 00:00:00

abstract：AIMS:To develop an intergeneric conjugation system for rimocidin-producing Streptomyces rimosus. METHODS AND RESULTS:High efficiencies of conjugation [10(-2)-10(-3) transconjugants/recipient colony forming units (CFU)] were obtained when spores of S. rimosus were heat treated at 40 degrees C for 10 min prior to mixing...

journal_title：Letters in applied microbiology

authors： Phornphisutthimas S,Sudtachat N,Bunyoo C,Chotewutmontri P,Panijpan B,Thamchaipenet A

更新日期：2010-05-01 00:00:00

abstract：:The detection of foodborne viruses in bivalve molluscs is a challenging procedure in relation to low virus concentration and to the presence of significant RT-PCR inhibitors. The aim of this study was the development of an efficient direct extraction method for foodborne viral RNA from bivalve molluscs. Using Mengovir...

journal_title：Letters in applied microbiology

authors： Quang Le H,Suffredini E,Tien Pham D,Kim To A,De Medici D

更新日期：2018-11-01 00:00:00

abstract：:To investigate the potential activation of hydrogen peroxide by a novel catalyst, reducing the concentration of hydrogen peroxide required and the time taken for mycobactericidal activity. The mycobactericidal properties of an iron-based novel heterogeneous-modified polyacrylonitrile (PAN) catalyst in combination with...

journal_title：Letters in applied microbiology

authors： Price SL,Huddersman KD,Shen J,Walsh SE

更新日期：2013-02-01 00:00:00

abstract：AIMS:Transmission of microbial pathogens to patients from water in dental units is a concern. To reduce this risk, the decontaminating efficiency of hydrogen peroxide was evaluated. METHODS AND RESULTS:Three percent hydrogen peroxide diluted 1 : 4 in distilled water (contact time 15 min) was used daily to disinfect th...

journal_title：Letters in applied microbiology

authors： Zanetti F,De Luca G,Tarlazzi P,Stampi S

更新日期：2003-01-01 00:00:00

abstract：AIMS:To measure the microbial removal capacity of a small-scale hydroponics wastewater treatment plant. METHODS AND RESULTS:Paired samples were taken from untreated, partly-treated and treated wastewater and analysed for faecal microbial indicators, i.e. coliforms, Escherichia coli, enterococci, Clostridium perfringen...

journal_title：Letters in applied microbiology

authors： Ottoson J,Norström A,Dalhammar G

更新日期：2005-01-01 00:00:00

abstract：AIMS:To investigate fusion expression between Bacillus thuringiensis crystal protein and a foreign protein, the expression of a fusion protein comprised of Cry1Ac, and enhanced green fluorescent protein (EGFP) in B. thuringiensis Cry(-)B strain was examined. METHODS AND RESULTS:The N-terminal fusion expression of EGFP...

journal_title：Letters in applied microbiology

authors： Roh JY,Li MS,Chang JH,Choi JY,Shim HJ,Shin SC,Boo KS,Je YH

更新日期：2004-01-01 00:00:00

abstract：AIMS:To evaluate the specificity and sensitivity of PCR primers for the detection of Salmonella enterica in a real-time PCR assay using pure cultures. METHODS AND RESULTS:Unenriched whole cells in sterile water were used as template for each PCR. SYBR Green dye was used for the nonspecific detection of dsDNA. The real...

journal_title：Letters in applied microbiology

authors： Csordas AT,Barak JD,Delwiche MJ

更新日期：2004-01-01 00:00:00

abstract：AIMS:The study aim was to determine the presence of total and faecal coliforms on kitchen surfaces, in tap water and on the hands of caregivers in households on both sides of the US-Mexico border. METHODS AND RESULTS:Samples were collected in 135 randomly selected households in Ciudad Juarez, Mexico, and El Paso, Texa...

journal_title：Letters in applied microbiology

authors： Carrasco L,Mena KD,Mota LC,Ortiz M,Behravesh CB,Gibbs SG,Bristol JR,Mayberry L,Cardenas VM

更新日期：2008-06-01 00:00:00

abstract：:Five phage types have been identified in 38 strains of Salmonella enteritidis isolated in Turkey in the 20-month period June 1992-January 1994. Strains belonging to phage type 4 predominated. Within phage type 4, plasmid profile typing has proved a useful method of strain discrimination and has confirmed the identity ...

journal_title：Letters in applied microbiology

authors： Erdem B,Threlfall EJ,Schofield SL,Ward LR,Rowe B

更新日期：1994-10-01 00:00:00