Development of a highly specific diagnostic 23S rDNA oligonucleotide probe for Mycobacterium leprae.

abstract：AIMS:In this study, we compared different methods of colistin susceptibility testing, disc diffusion, agar dilution and Etest using a set of Enterobacteriaceae isolates that included colistin-resistant strains. METHODS AND RESULTS:Susceptibility of 200 clinical isolates of Enterobacteriaceae to colistin was tested to ...

journal_title：Letters in applied microbiology

authors： Maalej SM,Meziou MR,Rhimi FM,Hammami A

更新日期：2011-11-01 00:00:00

abstract：UNLABELLED:Cryptococcosis, a fungal infection that affects both immunocompromised and immunocompetent individuals, contributes to increasing indices of mortality and morbidity. The development of resistance by Cryptococcus spp., the limited number of commercial antifungal drugs and the various side effects of these dru...

journal_title：Letters in applied microbiology

authors： Magalhães TF,da Silva CM,de Fátima Â,da Silva DL,Modolo LV,Martins CV,Alves RB,Ruiz AL,Longato GB,de Carvalho JE,de Resende-Stoianoff MA

更新日期：2013-08-01 00:00:00

abstract：AIMS:The genetic diversity of Vibrio cholerae isolated from black tiger shrimp (Penaeus monodon) aquaculture farms was determined using three PCR typing methods based on enterobacterial repetitive intergenic consensus (ERIC) sequences, ribosomal gene spacer (RS) sequence and repetitive extragenic palindromic (REP) sequ...

journal_title：Letters in applied microbiology

authors： Rao BM,Surendran PK

更新日期：2010-07-01 00:00:00

abstract：AIMS:To improve the production of sweet-tasting protein brazzein in Lactococcus lactis using controlled fermentation conditions. METHODS AND RESULTS:The nisin-controlled expression system was used for brazzein expression. The concentration of nisin for induction and the optical density (OD) at induction were therefore...

journal_title：Letters in applied microbiology

authors： Berlec A,Tompa G,Slapar N,Fonović UP,Rogelj I,Strukelj B

更新日期：2008-02-01 00:00:00

abstract：:Natural enzyme inhibitors have been widely described in literature because of its pharmacological and cosmetic applications. Fungi found in caves represent a promising source of bioactive substances that are still little explored scientifically. Thus, the present work evaluated the presence of enzymatic modulators in ...

journal_title：Letters in applied microbiology

authors： Cardoso MGB,Trento MVC,Reis CH,Marcussi S,Cardoso PG

更新日期：2019-08-01 00:00:00

abstract：:Listeria monocytogenes is a pathogenic bacterium, with human disease and infection linked to dairy products, seafood, ready-to-eat meat and raw & undercooked meats. Stainless steel is the most common food preparation surface and therefore, it is important to understand how food storage conditions such as surface mater...

journal_title：Letters in applied microbiology

authors： Redfern J,Verran J

更新日期：2017-04-01 00:00:00

abstract：AIM:To estimate the prevalence of thermotolerant Campylobacter spp. in commercially reared partridges (Perdix perdix) in southern Italy. METHODS AND RESULTS:Cloacal swabs of partridges (n = 240), equally distributed between male and female birds, from a game bird farm located in the Southern Italy were examined for th...

journal_title：Letters in applied microbiology

authors： Dipineto L,Gargiulo A,De Luca Bossa LM,Rinaldi L,Borrelli L,Santaniello A,Menna LF,Fioretti A

更新日期：2009-09-01 00:00:00

abstract：UNLABELLED:Aspergillus fumigatus, a well-known human and animal pathogen causing aspergillosis, has been historically identified by morphological and microscopic features. However, recent studies have shown that species identification on the basis of morphology alone is problematic. The aim of this work was to confirm ...

journal_title：Letters in applied microbiology

authors： Soleiro CA,Pena GA,Cavaglieri LR,Coelho I,Keller LM,Dalcero AM,Rosa CA

更新日期：2013-12-01 00:00:00

abstract：AIMS:To identify the abattoir source(s) of psychrophilic clostridia causing 'blown pack' spoilage of vacuum-packed chilled meats. METHODS AND RESULTS:Molecular procedures were used to detect the presence of specific 16S rRNA gene fragments of blown pack-causing clostridia in samples collected from a commercial abattoi...

journal_title：Letters in applied microbiology

authors： Boerema JA,Broda DM,Bell RG

更新日期：2003-01-01 00:00:00

abstract：AIMS:To examine the efficacy of liposome oral administration to induce systemic and mucosal immune responses against verotoxin-producing Escherichia coli (VTEC) and the effect of the induced antibodies on the binding of the bacteria to Caco-2 cells. METHODS AND RESULTS:Mice were immunized orally with VTEC antigen and ...

journal_title：Letters in applied microbiology

authors： Tana,Watarai S,Isogai E,Oguma K

更新日期：2003-01-01 00:00:00

abstract：:A new ultrasonic apparatus operating at a frequency of 40 kHz was developed to dislodge biofilms from food processing equipment in order to assess the effectiveness of cleaning protocols. Sonication conditions to remove biofilms and quantification by ATP-bioluminescence are described. An industrial meat process was de...

journal_title：Letters in applied microbiology

authors： Oulahal-Lagsir N,Martial-Gros A,Boistier E,Blum LJ,Bonneau M

更新日期：2000-01-01 00:00:00

abstract：:The use of buoyant density centrifugation (BDC) to prepare samples for PCR analysis of food pathogens is described. Blue cheese and milk homogenates were inoculated with Shigella flexneri and layered on top of Percoll media. After BDC, the food homogenates remained in the upper part of the centrifuge tube, separated f...

journal_title：Letters in applied microbiology

authors： Lindqvist R,Norling B,Lambertz ST

更新日期：1997-04-01 00:00:00

abstract：AIMS:This study was conducted to clarify the taxonomic status of Bacteroides type A strains with high vitamin B(12)-producing ability that is widely distributed in the intestinal tracts of freshwater fish. METHODS AND RESULTS:Seventeen strains of Bacteroides type A isolated from five fish species were all rod-shaped a...

journal_title：Letters in applied microbiology

authors： Tsuchiya C,Sakata T,Sugita H

更新日期：2008-01-01 00:00:00

abstract：:The novel Eriocheir sinensis reovirus (EsRV) is a pathogen that causes severe disease and high mortality rates in cultivated crabs. Here, we established a highly sensitive and specific rapid reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that was cheaper and more suitable for field applic...

journal_title：Letters in applied microbiology

authors： Ma Y,Dai T,Serwadda A,Shen H

更新日期：2016-11-01 00:00:00

abstract：AIM:The aim of this work was to evaluate the effect of Planococcus ficus infection in red wine grapes on Aspergillus section Nigri and ochratoxin A (OTA) contamination. METHODS AND RESULTS:During 2006/2007 and 2008/2009 vintages, Merlot, Malbec and Cabernet Sauvignon varieties divided into two categories of grape samp...

journal_title：Letters in applied microbiology

authors： Chiotta ML,Ponsone ML,Torres AM,Combina M,Chulze SN

更新日期：2010-08-01 00:00:00

abstract：:Candida albicans is an opportunistic fungus, which causes vulvovaginal candidiasis (VVC). The aim of this study was to evaluate Mrr2 mutation and its expression levels and Candida drug resistance 1 (Cdr1) in C. albicans associated with fluconazole (FCA) resistance. We identified 80 isolates of C. albicans from 155 vag...

journal_title：Letters in applied microbiology

authors： Feng W,Yang J,Ji Y,Xi Z,Yang L,Zhu X,Ma Y

更新日期：2020-02-01 00:00:00

abstract：AIMS:To characterize and identify a novel contaminant of aviation fuel. METHODS AND RESULTS:Micro-organisms (yeasts and bacteria) were isolated from samples of aviation fuel. A yeast that proved to have been unrecorded previously was isolated from more than one fuel sample. This novel yeast proved to be a new species ...

journal_title：Letters in applied microbiology

authors： Buddie AG,Bridge PD,Kelley J,Ryan MJ

更新日期：2011-01-01 00:00:00

abstract：AIMS:The objective of this study was to identify compounds responsible for medicinal off-flavours produced by different species and strains of Alicyclobacillus in orange juice using a combination of chromatographic-coupled olfactometric techniques and gas chromatography-mass spectrometry (GC-MS). METHODS AND RESULTS:E...

journal_title：Letters in applied microbiology

authors： Gocmen D,Elston A,Williams T,Parish M,Rouseff RL

更新日期：2005-01-01 00:00:00

abstract：AIMS:To investigate the ability of a mixture of phage K and six of its modified derivatives to prevent biofilm formation by Staphylococcus aureus and also to reduce the established biofilm density. METHODS AND RESULTS:The bioluminescence-producing Staph. aureus Xen29 strain was used in the study, and incubation of thi...

journal_title：Letters in applied microbiology

authors： Kelly D,McAuliffe O,Ross RP,Coffey A

更新日期：2012-04-01 00:00:00

abstract：:The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n = 4), V. alginolyticus (n = 11), V. diabolicus (n = 14) and V. harveyi (n = 3) were ...

journal_title：Letters in applied microbiology

authors： Dahanayake PS,Hossain S,Wickramanayake MVKS,Heo GJ

更新日期：2020-07-01 00:00:00

abstract：:The bacterial diversity and community structure involved in Chinese sauerkraut is one of the most important factors shaping the final characteristics of traditional foods. In this research, Lactobacillus casei 11MZ-5-1 was applied in Chinese sauerkraut fermentation as a starter culture. Illumina Miseq sequencing analy...

journal_title：Letters in applied microbiology

authors： Du R,Ge J,Zhao D,Sun J,Ping W,Song G

更新日期：2018-01-01 00:00:00

abstract：:Pyrimidine synthesis in the food spoilage agent Burkholderia cepacia ATCC 25416 was investigated. The five de novo pathway enzymes of pyrimidine biosynthesis were found to be active in B. cepacia ATCC 25416 and growth of this strain on uracil had an effect on the de novo enzyme activities. The in vitro regulation of a...

journal_title：Letters in applied microbiology

authors： Li K,West TP

更新日期：1995-11-01 00:00:00

abstract：AIMS:The objective of this study was to determine the antifungal activity of flurbiprofen against dermatophytes like Trichophyton, Microsporum and Epidermophyton species. METHODS AND RESULTS:Susceptibility tests were performed against dermatophytes like Trichophyton, Microsporum and Epidermophyton species by the micro...

journal_title：Letters in applied microbiology

authors： Chowdhury B,Adak M,Bose SK

更新日期：2003-01-01 00:00:00

abstract：AIMS:We report the analysis of genetic determinants conferring resistance to pristinamycin in Staphylococcus epidermidis strains and epidemiology typing of these strains by pulsed-field gel electrophoresis. METHODS AND RESULTS:Staphylococcus epidermidis (346 isolates) were searched for strains with pristinamycin resis...

journal_title：Letters in applied microbiology

authors： Achour W,Bouchami O,Galopin S,Leclercq R,Ben Hassen A

更新日期：2008-03-01 00:00:00

abstract：:From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown or...

journal_title：Letters in applied microbiology

authors： Ahrens P,Friis NF

更新日期：1991-06-01 00:00:00

abstract：:Increasingly, molecular methods have become important in identification and confirmation of bacteria at the species level. Rapid molecular methods provide sensitivity and specificity while reducing cost and resources. The primary goal of this study was to develop a real-time PCR assay for identification of Escherichia...

journal_title：Letters in applied microbiology

authors： Tillman GE,Simmons M,Wasilenko JL,Narang N,Cray WC Jr,Bodeis-Jones S,Martin G,Gaines S,Seal BS

更新日期：2015-02-01 00:00:00

abstract：:Colistin-resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high-resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known geno...

journal_title：Letters in applied microbiology

authors： Tahmasebi H,Dehbashi S,Arabestani MR

更新日期：2020-04-01 00:00:00

abstract：AIMS:We propose to apply the Wirtz-Conklin staining technique to evaluate spore germination. METHODS AND RESULTS:Spores at different stages of germination were stained with modified spore stain (Wirtz-Conklin) and evaluated for staining properties. Bacillus spores suspended in deionized water, which does not support g...

journal_title：Letters in applied microbiology

authors： Hamouda T,Shih AY,Baker JR Jr

更新日期：2002-01-01 00:00:00

abstract：:A sandwich enzyme-linked immunosorbent assay to detect staphylococcal enterotoxin B was developed using rat monoclonal antibodies as capture antibodies and as a biotinylated conjugate. This test was sensitive, less than 1 ng/ml of enterotoxin B was detected and interference by protein A was prevented by the use of rat...

journal_title：Letters in applied microbiology

authors： Rogemond V,Da Costa Castro JM,Delaunay T,Cazaux C,Guinet RM

更新日期：1991-09-01 00:00:00

abstract：UNLABELLED:Healthcare-associated infections (HCAI) affect 5-10% of acute hospital admissions. Environmental decontamination is an important component of all strategies to prevent HCAI as many bacterial causes survive and persist in the environment, which serve as ongoing reservoirs of infection. Current approaches such...

journal_title：Letters in applied microbiology

authors： Galvin S,Cahill O,O'Connor N,Cafolla AA,Daniels S,Humphreys H

更新日期：2013-08-01 00:00:00