Improved acid tolerance of a recombinant strain of Escherichia coli expressing genes from the acidophilic bacterium Oenococcus oeni.

abstract：UNLABELLED:The aim of this study was to assess the genetic diversity of Vibrio cholerae isolated from 2012 and 2013 outbreaks in Iran, with regard to their virulence properties. A total of 20 V. cholerae strains were collected from Sistan-Baluchestan province of Iran during 2012 and 2013 outbreaks. Hybridization assays...

journal_title：Letters in applied microbiology

authors： Bakhshi B

更新日期：2016-06-01 00:00:00

abstract：:Polyhydroxyalkanoates (PHAs) are polyesters accumulated by many bacteria under unbalanced growth conditions, and have been used to meet the various demands in areas of agriculture, medicine, and materials especially belong to a rapidly expanding area of biomedical research. Unfortunately, the high production cost than...

journal_title：Letters in applied microbiology

authors： Liu CC,Zhang LL,An J,Chen B,Yang H

更新日期：2016-01-01 00:00:00

abstract：AIMS:The aim of this study is to develop an RT-PCR assay combined with immunomagnetic beads (IMS/RT-PCR) coating monoclonal antibody (Mab) for separation and detection of norovirus (genogroup II) in faecal samples. We furthermore compare its detection limits with IMS/RT-PCR using polyclonal antibody (Pab) and the TRIzo...

journal_title：Letters in applied microbiology

authors： Yao L,Wu Q,Wang D,Kou X,Zhang J

更新日期：2009-08-01 00:00:00

abstract：:Campylobacter jejuni was inoculated into a range of raw and ready-to-eat foods. These food samples were used as a test source for detection of this bacterium by the polymerase chain reaction (PCR). Specific detection of Camp. jejuni, as indicated by a PCR product of 159 bp, was possible with all pre-cooked deli-sliced...

journal_title：Letters in applied microbiology

authors： Winters DK,O'Leary AE,Slavik MF

更新日期：1998-09-01 00:00:00

abstract：:Chronic Pseudomonas aeruginosa infection is associated with increased morbidity and mortality in patients with cystic fibrosis (CF). Current understanding of risk factors for acquisition is limited and so the aim of this study was to examine a large sample of environmental waters from diverse sources. Environmental wa...

journal_title：Letters in applied microbiology

authors： Caskey S,Stirling J,Moore JE,Rendall JC

更新日期：2018-06-01 00:00:00

abstract：:Non-degradability of conventional plastics, filling of landfill sites, raising water and land pollution and rapid depletion of fossil resources have raised the environmental issues and global concerns. The current demand and production of plastics is putting immense pressure on fossil resources, consuming about 6% of ...

journal_title：Letters in applied microbiology

authors： Satti SM,Shah AA

更新日期：2020-06-01 00:00:00

abstract：:The yield of purified recombinant alpha-sarcin increases approximately three- to fourfold when this toxin is co-expressed in Escherichia coli with thioredoxin. This increased production is attributed to the existence, in the presence of thioredoxin, of a reducing environment which allows rearrangement of incorrect dis...

journal_title：Letters in applied microbiology

authors： García-Ortega L,Lacadena J,Lacadena V,Masip M,De Antonio C,Martínez-Ruiz A,Martínez Del Pozo A

更新日期：2000-04-01 00:00:00

abstract：:Two commercially available screening methods, an automated enzyme-linked fluorescent immunoassay (VIDAS E. coli O157) and an immunomagnetic separation followed by culture onto cefixime tellurite sorbitol MacConkey agar (CT-SMAC), were compared for detection of Escherichia coli O157 in naturally and artificially contam...

journal_title：Letters in applied microbiology

authors： Vernozy-Rozand C,Mazuy C,Ray-Gueniot S,Boutrand-Loeï S,Meyrand A,Richard Y

更新日期：1997-12-01 00:00:00

abstract：AIM:To evaluate the efficacy of electrochemically activated solution (ECASOL) in decontaminating Bacillus anthracis Ames and Vollum 1B spores, with and without changing the source water hardness and final ECASOL pH. METHODS AND RESULTS:Five different ECASOL formulations were generated, in which the source water hardne...

journal_title：Letters in applied microbiology

authors： Rogers JV,Ducatte GR,Choi YW,Early PC

更新日期：2006-11-01 00:00:00

abstract：:Four strains of Escherichia coli were tested for electroporation-induced plasmid transformation using a high voltage pulse in a square waveform. Conditions for optimal transformation were determined for each strain and transformation frequencies found to be comparable to those reported previously for E. coli with a si...

journal_title：Letters in applied microbiology

authors： Elvin S,Bingham AH

更新日期：1991-02-01 00:00:00

abstract：:Growth at low positive temperatures and induced phenotypic resistance to extreme cold temperature (freezing/thawing cycles) of Enterococcus faecalis were investigated. The effect of low temperatures on the specific growth rates was studied; use of Arrhenius profile and Ratkovsky 'square-root' model allowed determinati...

journal_title：Letters in applied microbiology

authors： Thammavongs B,Corroler D,Panoff JM,Auffray Y,Boutibonnes P

更新日期：1996-12-01 00:00:00

abstract：AIMS:To evaluate the accuracy of pyrosequencing for the description of Phytophthora communities in terms of taxa identification and risk of assignment for false Molecular Operational Taxonomic Units (MOTUs). METHODS AND RESULTS:Pyrosequencing of Internal Transcribed Spacer 1 (ITS1) amplicons was used to describe the s...

journal_title：Letters in applied microbiology

authors： Vettraino AM,Bonants P,Tomassini A,Bruni N,Vannini A

更新日期：2012-11-01 00:00:00

abstract：:A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference...

journal_title：Letters in applied microbiology

authors： Chang JH,Roh JY,Je YH,Park HW,Jin BR,Woo SD,Kang SK

更新日期：1998-05-01 00:00:00

abstract：AIMS:The study evaluated the efficacy of four Mycobacterium avium subsp. paratuberculosis (MAP) culture media in suppressing commonly used starter cultures and typical nonstarter microflora present during the manufacture and ripening of Cheddar cheese, with a view to identify a suitable medium for the enumeration of MA...

journal_title：Letters in applied microbiology

authors： Donaghy JA,Totton NL,Rowe MT

更新日期：2003-01-01 00:00:00

abstract：:A monoclonal antibody (mAb) has been produced to aflatoxin B1 (AF B1) after successful immunization of mice and fusion of sensitized spleen cells with myeloma cancer cells. The mice were immunized with AF B1-oxime-protein conjugate. Positive mAbs were screened using an indirect ELISA specific for AF B1. The selected m...

journal_title：Letters in applied microbiology

authors： Candlish AA,Smith JE,Stimson WH

更新日期：1990-04-01 00:00:00

abstract：AIMS:The study aim was to determine the presence of total and faecal coliforms on kitchen surfaces, in tap water and on the hands of caregivers in households on both sides of the US-Mexico border. METHODS AND RESULTS:Samples were collected in 135 randomly selected households in Ciudad Juarez, Mexico, and El Paso, Texa...

journal_title：Letters in applied microbiology

authors： Carrasco L,Mena KD,Mota LC,Ortiz M,Behravesh CB,Gibbs SG,Bristol JR,Mayberry L,Cardenas VM

更新日期：2008-06-01 00:00:00

abstract：AIMS:The aim of this study was to explore the occurrence of methicillin-resistant staphylococci in a large urban public transport system. METHODS AND RESULTS:Samples were taken from hand rails, which passengers hold onto when they are standing. In total, 1400 swabs taken from 55 vehicles (trolleybuses, trams and buses...

journal_title：Letters in applied microbiology

authors： Stepanović S,Cirković I,Djukić S,Vuković D,Svabić-Vlahović M

更新日期：2008-10-01 00:00:00

abstract：AIMS:The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. METHODS AND RESULTS:Antimicrobial and ...

journal_title：Letters in applied microbiology

authors： Gudiña EJ,Rocha V,Teixeira JA,Rodrigues LR

更新日期：2010-04-01 00:00:00

abstract：AIMS:To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination. METHODS AND RESULTS:Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences wer...

journal_title：Letters in applied microbiology

authors： Liu D,Swiatlo E,Austin FW,Lawrence ML

更新日期：2006-09-01 00:00:00

abstract：:The ability of Stenotrophomonas maltophilia strain VUN 10,003 to degrade and detoxify high molecular weight polycyclic aromatic hydrocarbons (PAHs) was evaluated in a basal liquid medium. Using high cell density inocula of strain VUN 10,003, the concentration of pyrene, fluoranthene, benz[a]anthracene, benzo[a]pyrene,...

journal_title：Letters in applied microbiology

authors： Juhasz AL,Stanley GA,Britz ML

更新日期：2000-05-01 00:00:00

abstract：AIMS:This study was aimed to investigate the effectiveness of high-pressure homogenization (HPH) against Alicyclobacillus acidoterrestris. METHODS AND RESULTS:The susceptibility of three different strains of A. acidoterrestris (DSMZ 2498, Gamma4 and c8) to HPH (500-1700 bar) was studied. The experiments were performed...

journal_title：Letters in applied microbiology

authors： Bevilacqua A,Cibelli F,Corbo MR,Sinigaglia M

更新日期：2007-10-01 00:00:00

abstract：:Fifty-five isolates of nine species and two species varieties (5 isolates each) of the Aspergillus niger group were tested for progesterone transformation. Generally, all isolates of the different species transformed progesterone to 21-hydroxyprogesterone. In addition, the isolates of seven species have the capacity t...

journal_title：Letters in applied microbiology

authors： Mostafa ME

更新日期：1995-04-01 00:00:00

abstract：:High performance thin layer chromatography with automated multiple development was used to determine 2,3-butanediol levels in wine produced by high and low acetoin-forming strains of Saccharomyces cerevisiae. An inverse correlation between acetoin and 2,3-butanediol content was found suggesting a leaky mutation in ace...

journal_title：Letters in applied microbiology

authors： Romano P,Suzzi G,Brandolini V,Menziani E,Domizio P

更新日期：1996-04-01 00:00:00

abstract：AIMS: The aim of this paper is to check the effect of salinity on the bioremediation process of petroleum hydrocarbons in the saline-alkaline soil. METHODS AND RESULTS: In this study, soil salinity was adjusted to different levels by water leaching method and the bioremediation process was conducted for 28 days. Soil ...

journal_title：Letters in applied microbiology

authors： Qin X,Tang JC,Li DS,Zhang QM

更新日期：2012-09-01 00:00:00

abstract：UNLABELLED:Sandalwood oil has been found in numerous therapeutic applications in traditional medicines such as Chinese traditional medicine and Ayurveda. However, there are no comparative accounts available in the literature that focused on in vitro and in vivo tree sample-derived extracts. Combined dichloromethane and...

journal_title：Letters in applied microbiology

authors： Misra BB,Dey S

更新日期：2012-12-01 00:00:00

abstract：AIMS:  The primary goal of this study was to characterize the existence of a functional c-di-GMP pathway in the bioleaching bacterium Acidithiobacillus ferrooxidans. METHODS AND RESULTS:  A bioinformatic search revealed that the genome sequence of At. ferrooxidans ATCC 23270 codes for several proteins involved in the ...

journal_title：Letters in applied microbiology

authors： Ruiz LM,Castro M,Barriga A,Jerez CA,Guiliani N

更新日期：2012-02-01 00:00:00

abstract：AIMS:A rapid and simple method for enumerating uninjured and sublethally injured bacterial cells, the twofold dilution method (2FD), was developed and evaluated. METHODS AND RESULTS:Following twofold serial dilution of samples in a 96 well microtiter plate, double strength selective broth or nonselective broth was add...

journal_title：Letters in applied microbiology

authors： Kang DH,Siragusa GR

更新日期：2001-09-01 00:00:00

abstract：UNLABELLED:This study reports the development of a loop-mediated isothermal amplification (LAMP) reaction for the detection of Pythium myriotylum. The primer set targeting the ITS sequence of P. myriotylum worked most efficiently at 60°C and allowed the detection of P. myriotylum DNA within 30 min by fluorescence monit...

journal_title：Letters in applied microbiology

authors： Fukuta S,Takahashi R,Kuroyanagi S,Ishiguro Y,Miyake N,Nagai H,Suzuki H,Tsuji T,Hashizume F,Watanabe H,Kageyama K

更新日期：2014-07-01 00:00:00

abstract：AIMS:To evaluate the genetic diversity within the species Paenibacillus polymyxa. METHODS:Southern hybridization was performed on 102 strains of P. polymyxa using DNA from the phage IPy1 as a probe. RESULTS:All 102 strains hybridized to phage IPy1 DNA. Data from different hybridization patterns obtained were used to ...

journal_title：Letters in applied microbiology

authors： Santos SC,Rodrigues Coelho MR,Seldin L

更新日期：2002-01-01 00:00:00

abstract：AIMS:  This paper utilized quantitative LC-MS/MS to profile the short-chain acyl-CoA levels of several strains of Escherichia coli engineered for heterologous polyketide production. To further compare and potentially expand the levels of available acyl-CoA molecules, a propionyl-CoA synthetase gene from Ralstonia solan...

journal_title：Letters in applied microbiology

authors： Armando JW,Boghigian BA,Pfeifer BA

更新日期：2012-02-01 00:00:00