Development of loop-mediated isothermal amplification assay for the detection of Pythium myriotylum.

abstract：:Knowledge of the composition of the colonic microbiota is important for our understanding of how the balance of these microbes is influenced by diet and the environment, and which bacterial groups are important in maintaining gut health or promoting disease. Molecular methodologies have advanced our understanding of t...

journal_title：Letters in applied microbiology

authors： Duncan SH,Louis P,Flint HJ

更新日期：2007-04-01 00:00:00

abstract：UNLABELLED:The mechanisms and organisms involved in the natural formation of volatile organohalogen compounds (VOX) are largely unknown. We provide evidence that the common and widespread soil bacterium Sinorhizobium meliloti strain 1021 is capable of producing up to 3338·6 ± 327·8 ng l(-1) headspace volume of chlorofo...

journal_title：Letters in applied microbiology

authors： Weigold P,Ruecker A,Jochmann M,Osorio Barajas XL,Lege S,Zwiener C,Kappler A,Behrens S

更新日期：2015-10-01 00:00:00

abstract：:SDS-PAGE fingerprinting of cell-wall proteins extracted from 119 strains belonging to different species of lactic acid bacteria have been compared. The method of extraction and electrophoretic separation utilized in this work was found to be a reliable and rapid way for characterizing thermophilic lactobacilli species...

journal_title：Letters in applied microbiology

authors： Gatti M,Fornasari E,Neviani E

更新日期：1997-11-01 00:00:00

abstract：:Leclercia adecarboxylata, a Gram-negative bacillus of family Enterobacteriaceae, is an uncommonly identified pathogen isolated from environmental and clinical specimens. Most of the human infections are polymicrobial and commonly occur in immunocompromised hosts, although nosocomial infections in immunocompetent hosts...

journal_title：Letters in applied microbiology

authors： Choudhary M,Choudhary BK,Bhoyar S,Kale SB,Chaudhari SP,Bera BC,Jain A,Barbuddhe SB

更新日期：2018-01-01 00:00:00

abstract：UNLABELLED:Staphylococcus aureus LZ-01 was isolated from the Yellow River upstream from Lanzhou which can resist and reduce chromium (VI) to chromium (III). In this study, strain LZ-01's uranium (VI) resistance and adsorption abilities were investigated. Our results showed that it can resist 2 mmol l(-1) U(VI) and adso...

journal_title：Letters in applied microbiology

authors： Zou L,Chen Z,Zhang X,Liu P,Li X

更新日期：2014-11-01 00:00:00

abstract：AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spore...

journal_title：Letters in applied microbiology

authors： Vepachedu VR,Hirneisen K,Hoover DG,Setlow P

更新日期：2007-09-01 00:00:00

abstract：AIM:The mechanisms of adaptation of Clostridium perfringens to high temperatures are not well understood. In this work, the involvement of extracellular compounds in protection to heat was determined. METHODS AND RESULTS:Cells were grown in fluid thioglycollate medium or chicken broth. When mid-log phase was reached, ...

journal_title：Letters in applied microbiology

authors： Heredia N,Ybarra P,Hernández C,García S

更新日期：2009-01-01 00:00:00

abstract：:Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses in dairy industry worldwide. In addition, milk and milk-products contaminated by Staph. aureus can cause harmful human diseases. The aim of this study was to characterize Staph. aureus strains isolated from dairy farms in...

journal_title：Letters in applied microbiology

authors： Ben Said M,Abbassi MS,Bianchini V,Sghaier S,Cremonesi P,Romanò A,Gualdi V,Hassen A,Luini MV

更新日期：2016-12-01 00:00:00

abstract：AIMS:  We evaluated risks associated with diaper changing in Finnish kindergartens where children were using either modern disposable paper or reusable cloth diapers. METHODS AND RESULTS:  We determined enteric micro-organisms and ammonia in diaper-changing rooms in four kindergartens in autumn and winter in the ambie...

journal_title：Letters in applied microbiology

authors： Vaattovaara PE,Kivimäenpää M,Vaattovaara P,Pasanen P,Heinonen-Tanski H

更新日期：2012-05-01 00:00:00

abstract：:The effect of relative humidity (RH) on the antimicrobial efficacy of chlorine dioxide (ClO2 ) gas against foodborne pathogens on tomatoes was evaluated. Also, levels of ClO2 residues on tomatoes after exposure to ClO2 gas under different RH conditions were measured to determine the quantity of solubilized ClO2 gas on...

journal_title：Letters in applied microbiology

authors： Park SH,Kim WJ,Kang DH

更新日期：2018-08-01 00:00:00

abstract：:Enterococci were isolated from faecal droppings of chickens in broiler and layer farms and the susceptibilities to nine therapeutic antimicrobial agents and six growth-promoting antibiotics were determined by the agar dilution method. Resistance to therapeutic antimicrobial agents such as ampicillin, clindamycin, eryt...

journal_title：Letters in applied microbiology

authors： Yoshimura H,Ishimaru M,Endoh YS,Kojima A

更新日期：2000-12-01 00:00:00

abstract：AIMS:To test a performance of the microbiological safety cabinets (MSCs) according to the type of MSCs in microbial laboratories. METHODS AND RESULTS:Tests were carried out to assess the performance of 31 MSCs in 14 different facilities, including six different biological test laboratories in six hospitals and eight d...

journal_title：Letters in applied microbiology

authors： Hwang SH,Yi TW,Cho KH,Lee IM,Yoon CS

更新日期：2011-09-01 00:00:00

abstract：AIM:To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures. METHODS AND RESULTS:Stationary-phase E. coli cells were inactivated at 52, 54 and 58°C. The heat resistance is described as reduction in the inactivation rate, k(max) , and...

journal_title：Letters in applied microbiology

authors： Velliou EG,Van Derlinden E,Cappuyns AM,Aerts D,Nikolaidou E,Geeraerd AH,Devlieghere F,Van Impe JF

更新日期：2011-02-01 00:00:00

abstract：AIMS:To provide comparative genome sequence data for two related model strains of Staphylococcus aureus (SH1000 and 8325-4) that are used extensively in laboratory research. METHODS AND RESULTS:Comparative genome sequencing was used to identify genetic differences between Staph. aureus SH1000 and the fully genome-sequ...

journal_title：Letters in applied microbiology

authors： O'Neill AJ

更新日期：2010-09-01 00:00:00

abstract：AIMS:In this study, a capillary polymerase chain reaction (cPCR) was applied for Salmonella detection from poultry meat. METHODS AND RESULTS:Salmonella detection limits of the optimized cPCR were determined with DNA templates from the samples of tetrathionate broth (TTB), Rappaport Vassiliadis broth (RVB) and selenite...

journal_title：Letters in applied microbiology

authors： Gunaydin E,Eyigor A,Carli KT

更新日期：2007-01-01 00:00:00

abstract：AIMS:To develop a specific, fast and simple molecular method useful to detect the entomopathogenic bacterium Pseudomonas entomophila. METHODS AND RESULTS:The use of bioinformatics tools allowed the identification of unique genes present in P. entomophila genome. Using such genes, we designed primers aiming to detect s...

journal_title：Letters in applied microbiology

authors： Papagiannoulis A,Mathiopoulos KD,Mossialos D

更新日期：2010-03-01 00:00:00

abstract：:This study was undertaken to detect the occurrence of beta-lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n = 92) and backyard (n = 110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87...

journal_title：Letters in applied microbiology

authors： Banerjee A,Bardhan R,Chowdhury M,Joardar SN,Isore DP,Batabyal K,Dey S,Sar TK,Bandyopadhyay S,Dutta TK,Samanta I

更新日期：2019-08-01 00:00:00

abstract：AIMS:Food-borne pathogen inhibition was tested in the presence of a mixture of Lactobacillus acidophilus and Lactobacillus casei during fermentation under controlled pH conditions. METHODS AND RESULTS:The growth of Escherichia coli O157:H7, Salmonella serotype Typhimurium, Staphylococcus aureus, Listeria innocua, Ente...

journal_title：Letters in applied microbiology

authors： Millette M,Luquet FM,Lacroix M

更新日期：2007-03-01 00:00:00

abstract：AIMS:To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming. METHODS AND RESULTS:The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously con...

journal_title：Letters in applied microbiology

authors： Dong H,Tao W,Zhu L,Zhang Y,Li Y

更新日期：2011-09-01 00:00:00

abstract：:The aim of this study was to determine the epidemiology (location, microbial load, microbiome, presence/absence of biofilm and pathogens, including ESKAPE-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter species, and antimicrobial susc...

journal_title：Letters in applied microbiology

authors： Costa DM,Johani K,Melo DS,Lopes LKO,Lopes Lima LKO,Tipple AFV,Hu H,Vickery K

更新日期：2019-04-01 00:00:00

abstract：:Random amplification of polymorphic DNA (RAPD) is proving to be a useful technique in studying the epidemiology of micro-organisms. The technique can be troublesome and time consuming to establish due to the essentially empirical approach to optimization. By standardization of certain parameters and use of a commercia...

journal_title：Letters in applied microbiology

authors： Hilton AC,Banks JG,Penn CW

更新日期：1997-04-01 00:00:00

abstract：:While the beta-glucuronidase activity of intact cells of Clostridium perfringens was higher in 0.95% sodium chloride (NaCl) than that in 0, 0.1 or 0.5%, that of Escherichia coli was higher in 0.1% NaCl than that in 0, 0.5 or 0.95% NaCl in 0.1 mol l-1 KH2PO4. However, the enzyme activity of both species of intact cells...

journal_title：Letters in applied microbiology

authors： Fujisawa T,Aikawa K,Takahashi T,Yamai S

更新日期：2000-09-01 00:00:00

abstract：AIMS:Oenococcus oeni is a lactic acid bacterium used in wine fermentation. Two open reading frames (orfB and orfC) were identified in the upstream region of the hsp18 gene, encoding the small heat-shock protein Lo18. Expression of these genes in conditions of acid stress was studied in Escherichia coli. METHODS AND RE...

journal_title：Letters in applied microbiology

authors： Morel F,Delmas F,Jobin MP,Diviès C,Guzzo J

更新日期：2001-08-01 00:00:00

abstract：:From a genomic library of Mycoplasma hyopneumoniae a 1.3 kb DNA fragment was cloned which showed specific Southern hybridization and dot hybridization with the type strain of several porcine and bovine Mycoplasma species. This probe selectively recognized M. hyopneumoniae sequences in purified DNA or in broth-grown or...

journal_title：Letters in applied microbiology

authors： Ahrens P,Friis NF

更新日期：1991-06-01 00:00:00

abstract：AIMS:In this study, the relationship between morphology and cephalosporin C (CPC) production in a 30-l bioreactor culture of Cephalosporium acremonium M25 using a 3:7 seed mixture was investigated. In addition, the kinetic model was established and applied. METHODS AND RESULTS:CPC production was performed in a 30-l bi...

journal_title：Letters in applied microbiology

authors： Kim JH,Lim JS,Kim CH,Kim SW

更新日期：2005-01-01 00:00:00

abstract：AIMS:To investigate the influence of the growth phase, growth temperature, storage time, pH and aw of the treatment medium on the resistance of Listeria monocytogenes to pulsed electric fields (PEF). METHODS AND RESULTS:Square wave pulses of 2 micros at a frequency of 1 Hz and 25 and 28 kV cm(-1) were used. Cells were...

journal_title：Letters in applied microbiology

authors： Alvarez I,Pagán R,Raso J,Condón S

更新日期：2002-01-01 00:00:00

abstract：AIM:To evaluate the effect of starvation, heat, cold, acid, alkaline, chlorine and ethanol stresses on the resistance of Enterobacter sakazakii in powdered infant milk formula (PIMF) towards gamma radiation. METHODS AND RESULTS:Stressed cells of E. sakazakii ATCC 51329 and four other food isolate strains were mixed in...

journal_title：Letters in applied microbiology

authors： Osaili T,Al-Nabulsi A,Shaker R,Ayyash M,Olaimat A,Abu Al-Hasan A,Kadora K,Holley R

更新日期：2008-08-01 00:00:00

abstract：AIMS:To investigate interactions between rumen protozoa and Shiga toxin-producing Escherichia coli (STEC) and to ascertain whether it is likely that rumen protozoa act as ruminant hosts for STEC. METHODS AND RESULTS:The presence of stx genes in different microbial fractions recovered from cattle and sheep rumen conten...

journal_title：Letters in applied microbiology

authors： Burow LC,Gobius KS,Vanselow BA,Klieve AV

更新日期：2005-01-01 00:00:00

abstract：:Heat-resistant fungi occur sporadically and are a continuing problem for the food and beverage industry. The genus Talaromyces, as a typical fungus, is capable of producing the heat-resistant ascospores responsible for the spoilage of processed food products. Isocitrate lyase, a signature enzyme of the glyoxylate cycl...

journal_title：Letters in applied microbiology

authors： Yamashita S,Nakagawa H,Sakaguchi T,Arima TH,Kikoku Y

更新日期：2018-01-01 00:00:00

abstract：:Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

journal_title：Letters in applied microbiology

authors： Wang SQ,Wang T,Liu JF,Deng L,Wang F

更新日期：2018-11-01 00:00:00