Anomalies in species identification of enterococci from veterinary sources using a commercial biochemical identification system.

Abstract:

AIMS:A commercial biochemical panel ID kit was used to identify presumptive enterococci isolates of veterinary or agricultural origin obtained during different steps of culture. METHODS AND RESULTS:Fifty isolates identified as enterococci using a genus PCR assay were tested for genus and species identification using the BBL Crystal Identification Gram-Positive ID kit (Becton Dickinson, Sparks, MD, USA). Following sub-culture of the isolates three times, 59% agreement with the original panel ID was obtained. After four and six sub-cultures, percentage agreement increased to 61 and 64%, respectively. Nineteen of the 50 cultures were identified as both Enterococcus faecalis and E. faecium. CONCLUSIONS:As a result of the variability between speciation of isolates following re-culture, additional methods for speciation are warranted. SIGNIFICANCE AND IMPACT OF THE STUDY:This study suggests that the identification of the genus and species of non-human enterococcal isolates can vary greatly during successive passages when using this kit.

journal_name

Lett Appl Microbiol

authors

Hudson CR,Fedorka-Cray PJ,Jackson-Hall MC,Hiott LM

doi

10.1046/j.1472-765x.2003.01302.x

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

245-50

issue

4

eissn

0266-8254

issn

1472-765X

pii

1302

journal_volume

36

pub_type

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