Abstract：
:In this study, a total of 172 putative omega-3 producers were isolated from 28 sediment samples from the Arabian Gulf employing a selective isolation procedure using marine agar containing 0·1% triphenyl tetrazolium chloride (TTC). Out of these 172 isolates, 19 isolates produced eicosapentaenoic acid (EPA) as confirmed by Gas Chromatography-Mass Spectrometry (GC-MS). The EPA content of the isolated bacterial strain varied from 1·76 to 6·52% of total fatty acids. Among the 19 isolates of EPA producers, while 17 isolates harboured both pfaA gene and Δ6 desaturase gene, only five isolates harboured Δ5 desaturase gene. Two of the EPA positive strains harbour none of the three genes tested. The 16s RNA identification of these isolates revealed that except one, all the EPA producers were Gram-positive marine bacteria belonging to the phylum Firmicutes, family Bacillacea, genera Bacillus and Oceanobacillus. Halomonas pacifica was the only Gram-negative Gamma-Proteobacteria detected to produce EPA from this region. SIGNIFICANCE AND IMPACT OF THE STUDY: Recently, marine bacteria are considered as a promising source of polyunsaturated fatty acid (PUFA) over marine fishes and microalgae. PUFA producers reported from polar and deep-sea sources were restricted to five well-known marine genera under two distinct domains of bacteria such as proteobacteria (Shewanella, Colwellia, and Moritella) and cytophaga group (Flexibacter, Psychroflexus). This study revealed that subtropical marine environment could also be the source of PUFA producing bacteria, and they predominantly belonged to the class of Firmibacteria. This finding opens up new avenue for research to study the inherent mechanism and physiology of such organisms from this unique environment.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Alagarsamy S,Sabeena Farvin KH,Fakhraldeen S,Kooramattom MR,Al-Yamani Fdoi
10.1111/lam.13186subject
Has Abstractpub_date
2019-08-01 00:00:00pages
121-127issue
2eissn
0266-8254issn
1472-765Xjournal_volume
69pub_type
杂志文章abstract：:Seventeen aerobic bacterial strains known to degrade one or more aromatic compounds were tested for their ability to utilize benzene, seven different methylated benzenes or other aromatic compounds as their sole source of carbon. The results are discussed with respect to the possibility of finding strains which degrad...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1996.tb00078.x
更新日期：1996-10-01 00:00:00
abstract：AIMS:To examine the ability of Agrobacterium to attach to Metarhizium anisopliae var. acridum strain CG423 under co-cultivation and to develop an Agrobacterium-mediated method of gene delivery into strain CG423, a promising agent for biological control of grasshoppers. METHODS AND RESULTS:The co-cultivation of Agrobac...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.02092.x
更新日期：2007-03-01 00:00:00
abstract：UNLABELLED:Cryptococcosis, a fungal infection that affects both immunocompromised and immunocompetent individuals, contributes to increasing indices of mortality and morbidity. The development of resistance by Cryptococcus spp., the limited number of commercial antifungal drugs and the various side effects of these dru...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12086
更新日期：2013-08-01 00:00:00
abstract：:The pattern of DNA fragments produced by the polymerase chain reaction using arbitrary primers is strongly influenced by the age of the colonies sampled. We show that the number, clarity and reproducibility of the bands produced is optimal when very young colonies are sampled. ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1993.tb01467.x
更新日期：1993-12-01 00:00:00
abstract：AIMS:To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO(2) ) and dry heat in reducing the number of Escherichia coli O157:H7. METHODS AND RESULTS:Radish seeds containing E. coli O157:H7 at 5.5 log CFU g(-1) were treated with 500 μg ml(-1) ClO(2) for 5 min and subseq...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2011.03125.x
更新日期：2011-10-01 00:00:00
abstract：AIMS:To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming. METHODS AND RESULTS:The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously con...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2011.03111.x
更新日期：2011-09-01 00:00:00
abstract：:A Multiplex PCR-based assay (m-PCR) with three sets of primers was developed for the detection of all serotypes of Salmonella enterica and the identification of Salmonella Enteritidis and Salmonella Typhimurium. This method was evaluated against a bacteriological method for the analysis of environmental swabs of poult...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1365-2672.1999.00488.x
更新日期：1999-02-01 00:00:00
abstract：AIMS:The aim of this work was to optimize the production of a novel antimaralial menisporopsin A by the seed fungus Menisporopsis theobromae BCC 4162. METHODS AND RESULTS:Fungal cultures were grown in shake flasks at 25 degrees C in the basal medium with varying carbon and nitrogen sources, aeration rates and initial ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.01994.x
更新日期：2006-11-01 00:00:00
abstract：AIMS:To investigate the use of mannitol salt agar (MSA) supplemented with acriflavine for selective growth and quantification of Staphylococcus aureus from flushed dairy manure wastewater (FDMW). METHODS AND RESULTS:Minimal inhibitory concentrations of acriflavine in MSA were determined by comparing the growth of S. a...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.01915.x
更新日期：2006-06-01 00:00:00
abstract：:The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n = 4), V. alginolyticus (n = 11), V. diabolicus (n = 14) and V. harveyi (n = 3) were ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13232
更新日期：2020-07-01 00:00:00
abstract：:Escherichia coli O157:H7 (O157) has unusual acid tolerance. The influence of heat shock on acid tolerance of O157 was studied. Seven strains of O157 and E. coli K-12 were tested for their ability to survive in minimum glucose medium (pH 2.5) at 37 degrees C. The survival of heat-shocked (10 min at 48 degrees C) cells ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1998.00264.x
更新日期：1998-01-01 00:00:00
abstract：AIMS:To elucidate the molecular mechanisms associated with mycoparasitism from Chaetomium cupreum, an effective biocontrol agent with ability against plant pathogenic fungi. METHODS AND RESULTS:One cDNA library was constructed from conditions predicted to resemble mycoparasitic process. A total of 1876 ESTs were gener...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2008.02487.x
更新日期：2009-03-01 00:00:00
abstract：UNLABELLED:Most plant species are colonized by endophytic bacteria. Despite their importance for plant health and growth, the response of these bacteria to grassland management regimes is still not understood. Hence, we investigated the bacterial community structure in three agricultural important grass species Dactyli...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12551
更新日期：2016-04-01 00:00:00
abstract：UNLABELLED:The influence of oenological factors on cold-active pectinases from 15 preselected indigenous yeasts belonging to Aureobasidium pullulans, Filobasidium capsuligenum, Rhodotorula dairenensis, Cryptococcus saitoi and Saccharomyces cerevisiae was investigated. Pectinolytic enzymes were constitutive or partially...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12390
更新日期：2015-05-01 00:00:00
abstract：AIMS:To test the suitability of cheese whey powder (CWP) solution for ethanol fermentation and to compare performances of different Kluyveromyces marxianus strains for ethanol fermentation from CWP solution. METHODS AND RESULTS:Batch ethanol fermentation of cheese whey (CW), CWP and lactose solutions with the same ini...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2007.02132.x
更新日期：2007-06-01 00:00:00
abstract：:Sixteen Bifidobacterium isolates from the human gastrointestinal tract were assayed for susceptibility to 44 antibiotics by soft agar overlay disc diffusion on TPY agar. Five isolates (3/7 B. bifidum and 2/3 B. breve) exhibited atypical antibiotic susceptibility profiles. Poor growth in the agar overlay accounted for ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1998.00342.x
更新日期：1998-05-01 00:00:00
abstract：:Fifty-five isolates of nine species and two species varieties (5 isolates each) of the Aspergillus niger group were tested for progesterone transformation. Generally, all isolates of the different species transformed progesterone to 21-hydroxyprogesterone. In addition, the isolates of seven species have the capacity t...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1995.tb00438.x
更新日期：1995-04-01 00:00:00
abstract：:Flow cytometry in combination with fluorescent molecular markers 5- (and 6-) carboxyfluorescein succinimidylester (CFSE) and propidium iodide (PI) have been applied to determine lag times, numbers of cell divisions and injury after mild heat (50 degrees C, 5 min) and nisin treatments (0.1 and 1.0 microgram ml-1) of La...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1997.00225.x
更新日期：1997-10-01 00:00:00
abstract：:Three groups of substances were identified which inhibit the bactericidal activity of bacitracin. Beside two divalent metals, Mg2+ and Ca2+, and a metabolite with chelating properties, citrate, the most pronounced effect was observed with pyrophosphate. Metals probably prevent access of bacitracin to the lipid carrier...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1994.tb00916.x
更新日期：1994-08-01 00:00:00
abstract：AIMS:The current study examined the antimicrobial susceptibility of 86 Listeria spp. isolated from processed bison carcasses. MATERIALS AND METHODS:Susceptibility to 25 antimicrobial agents was determined using E-test and National Antimicrobial Resistance Monitoring System (NARMS) panels. Most Listeria isolates (88-98...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765X.2006.02027.x
更新日期：2007-01-01 00:00:00
abstract：UNLABELLED:Faecal indicator bacteria (FIB) and harmful algal blooms (HABs) threaten the health and the economy of coastal communities worldwide. Emerging automated sampling technologies combined with molecular analytical techniques could enable rapid detection of micro-organisms in-situ, thereby improving resource mana...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12432
更新日期：2015-08-01 00:00:00
abstract：AIMS:To develop an assay for rapid screening of bacterial adhesion to various groups of biomolecules present in fish mucus. METHODS AND RESULTS:A novel assay was developed for investigation of bacterial adhesion to various groups of mucus biomolecules from fish. Lipid-, protein-, carbohydrate- and nucleic acid-rich co...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.12003
更新日期：2013-01-01 00:00:00
abstract：AIMS:A simplified and rapid method was developed to identify and enumerate total mesophilic microbial load, gram-negative bacteria, coliforms, and Escherichia coli in ground beef, using a single module. METHODS AND RESULTS:A 96-well microtiter plate was divided into four sections of two lanes (24 wells), with each sec...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01273.x
更新日期：2003-01-01 00:00:00
abstract：:The beta-D-galactosidase activity of viable but non-culturable (vnc) Escherichia coli cells in seawater was investigated using a rapid fluorimetric enzyme assay. Results from microcosm studies showed that loss of culturability did not necessarily result in loss of the ability to produce the galactosidase enzyme. Even ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1995.tb01016.x
更新日期：1995-08-01 00:00:00
abstract：:During investigation of a gastroenteritis outbreak in a chronic care institution, Norwalk virus was found in stool specimens from two individuals and bacterial isolates presumptively identified as Bacillus cereus were isolated from four individuals (including one with Norwalk virus) and spice. Phage typing confirmed a...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1995.tb01017.x
更新日期：1995-08-01 00:00:00
abstract：:A Coxiella sp. closely related to the agent of Q-fever, Coxiella burnetii, has been associated with mortalities in redclaw crayfish, (Cherax quadricarinatus), in farms and experimental facilities for three decades. Limited sequence data including 16S rRNA have placed the rickettsial species as a new species, candidatu...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/lam.13336
更新日期：2020-09-01 00:00:00
abstract：AIMS:The following polymers were developed: polyethylene (PE), a PE and polyethylene oxide (70% PE and 30% PEO; PE + PEO) blend, PE and nisin (PE + nisin), PE, nisin, and EDTA (PE + nisin + EDTA), and PE + PEO with nisin (PE + PEO + nisin). METHODS AND RESULTS:Of the polymers tested, PE and PE + PEO did not exhibit an...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2001.01005.x
更新日期：2001-10-01 00:00:00
abstract：AIMS:To examine factors affecting faecal shedding of the foodborne pathogens Escherichia coli O157:H7 and Salmonella in dairy cattle and evaluate antimicrobial susceptibility of these isolates. METHODS:Faecal samples were obtained in replicate from lactating (LAC; n = 60) and non-lactating (NLAC; n = 60) Holstein catt...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.2003.01417.x
更新日期：2003-01-01 00:00:00
abstract：:The use of energy dispersive analysis of X-rays (EDAX) to identify and quantify the presence of chlorhexidine diacetate (CHA) within Saccharomyces cerevisiae cells was examined. Chlorine was used as the elemental marker tag. Saccharomyces cerevisiae cells exposed to 1000 micrograms ml-1 CHA took up increasing amounts ...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1111/j.1472-765x.1995.tb01319.x
更新日期：1995-06-01 00:00:00
abstract：:A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference...
journal_title：Letters in applied microbiology
pub_type： 杂志文章
doi：10.1046/j.1472-765x.1998.00355.x
更新日期：1998-05-01 00:00:00
