Improved genetic transformation methods for the model alkaliphile Bacillus halodurans C-125.

abstract：:Novel findings in microbiology question the long-standing paradigm that a healthy pregnancy implies a sterile uterus. It now seems that the placenta is frequently colonized with bacteria, and a placental microbiome has been identified. Recent literature findings are summarized here, and an attempt is made to separate ...

journal_title：Letters in applied microbiology

authors： Wassenaar TM,Panigrahi P

更新日期：2014-12-01 00:00:00

abstract：:A wild type micro-organism producing antibacterial substances has been isolated from a Chinese fermented soybean seasoning and identified as Bacillus subtilis. A crude antibacterial preparation (CABP) was obtained by ammonium sulphate precipitation. Isoelectric focusing assay revealed at least four antimicrobial compo...

journal_title：Letters in applied microbiology

authors： Zheng G,Slavik MF

更新日期：1999-05-01 00:00:00

abstract：:Ergosterol biosynthesis in Saccharomyces cerevisiae is complex and the underlying mechanism of regulation remains unclear. To clarify the influence of transcriptional regulation on the ergosterol content, transcription factor Ecm22 was overexpressed in S. cerevisiae. Results showed that the overexpression of ECM22 led...

journal_title：Letters in applied microbiology

authors： Wang SQ,Wang T,Liu JF,Deng L,Wang F

更新日期：2018-11-01 00:00:00

abstract：AIMS:Breast milk has been described as a source of bacteria influencing the development of the infant gut microbiota. Up to the present, few studies have been focused on the application of culture-independent techniques to study bacterial diversity in breast milk. In this context, the aim of this study was to character...

journal_title：Letters in applied microbiology

authors： Collado MC,Delgado S,Maldonado A,Rodríguez JM

更新日期：2009-05-01 00:00:00

abstract：:Yellow wine lees (YWL) are the main co-products in yellow wine industry with unbalanced amino acid (AA) profiles. Solid-state fermentation was employed in this study to upgrade the YWL for ruminant animals. A 3 × 3 orthogonal design was conducted to optimize the fermentation condition for optimal crude protein (CP) yi...

journal_title：Letters in applied microbiology

authors： Yao KY,Zhang TZ,Wang HF,Liu JX

更新日期：2018-12-01 00:00:00

abstract：:Three groups of substances were identified which inhibit the bactericidal activity of bacitracin. Beside two divalent metals, Mg2+ and Ca2+, and a metabolite with chelating properties, citrate, the most pronounced effect was observed with pyrophosphate. Metals probably prevent access of bacitracin to the lipid carrier...

journal_title：Letters in applied microbiology

authors： Podlesek Z,Comino A

更新日期：1994-08-01 00:00:00

abstract：AIMS:To establish a rapid and reliable multiplex PCR (mPCR)-based method allowing specific identification of Carnobacterium piscicola SF668 during storage of cold smoked salmon (CSS). METHODS AND RESULTS:CSS was inoculated with C. piscicola SF668 and stored at 4 degrees C. Samples were withdrawn at regular time interv...

journal_title：Letters in applied microbiology

authors： Pellé E,Dousset X,Prévost H,Drider D

更新日期：2005-01-01 00:00:00

abstract：UNLABELLED:The mechanisms and organisms involved in the natural formation of volatile organohalogen compounds (VOX) are largely unknown. We provide evidence that the common and widespread soil bacterium Sinorhizobium meliloti strain 1021 is capable of producing up to 3338·6 ± 327·8 ng l(-1) headspace volume of chlorofo...

journal_title：Letters in applied microbiology

authors： Weigold P,Ruecker A,Jochmann M,Osorio Barajas XL,Lege S,Zwiener C,Kappler A,Behrens S

更新日期：2015-10-01 00:00:00

abstract：AIMS:To provide comparative genome sequence data for two related model strains of Staphylococcus aureus (SH1000 and 8325-4) that are used extensively in laboratory research. METHODS AND RESULTS:Comparative genome sequencing was used to identify genetic differences between Staph. aureus SH1000 and the fully genome-sequ...

journal_title：Letters in applied microbiology

authors： O'Neill AJ

更新日期：2010-09-01 00:00:00

abstract：:Alzheimer's disease (AD) is the most common form of dementia and one of the major causes of disability and dependency in older people. Accumulating evidences link gut microbiota with different diseases and its relationship with neurodegenerative diseases is becoming most intriguing. This study was aimed to compare the...

journal_title：Letters in applied microbiology

authors： Bäuerl C,Collado MC,Diaz Cuevas A,Viña J,Pérez Martínez G

更新日期：2018-06-01 00:00:00

abstract：AIMS:Aeromonas hydrophila is recognized as a human pathogen following wound exposure or ingestion of contaminated water and food. For rapid identification of this bacterium, a TaqMan-based real-time PCR assay has been developed. METHODS AND RESULTS:Primers and probes that target specific sequences of the 16S rRNA gene...

journal_title：Letters in applied microbiology

authors： Trakhna F,Harf-Monteil C,Abdelnour A,Maaroufi A,Gadonna-Widehem P

更新日期：2009-08-01 00:00:00

abstract：UNLABELLED:In this study, we developed and validated a loop-mediated isothermal amplification (LAMP) assay for Salmonella detection targeting bcfD gene, a conserved fimbrial operon gene existing in Salmonella. The Salmonella LAMP assay we developed successfully amplified 44 Salmonella strains (14 standard strains and 3...

journal_title：Letters in applied microbiology

authors： Zhuang L,Gong J,Li Q,Zhu C,Yu Y,Dou X,Liu X,Xu B,Wang C

更新日期：2014-12-01 00:00:00

abstract：UNLABELLED:This is the first reported study on the immobilization of living recombinant Escherichia coli cells that overexpress cyclopentanone monooxygenase in polyvinyl alcohol gel particles LentiKats®. Immobilized cells overexpressing cyclopentanone monooxygenase have been used as a model of biocatalyst for enantiose...

journal_title：Letters in applied microbiology

authors： Rebroš M,Lipták L,Rosenberg M,Bučko M,Gemeiner P

更新日期：2014-06-01 00:00:00

abstract：AIMS:To examine the efficacy of liposome oral administration to induce systemic and mucosal immune responses against verotoxin-producing Escherichia coli (VTEC) and the effect of the induced antibodies on the binding of the bacteria to Caco-2 cells. METHODS AND RESULTS:Mice were immunized orally with VTEC antigen and ...

journal_title：Letters in applied microbiology

authors： Tana,Watarai S,Isogai E,Oguma K

更新日期：2003-01-01 00:00:00

abstract：:Colistin-resistant Pseudomonas aeruginosa (CRPA), as a health care system threat, is increasing globally. This study aimed was to determine CRPA by high-resolution melting curve (HRM) analysis method. The HRM method was done on standard strains of P. aeruginosa and CRPA strains. Ninefold serial dilutions of known geno...

journal_title：Letters in applied microbiology

authors： Tahmasebi H,Dehbashi S,Arabestani MR

更新日期：2020-04-01 00:00:00

abstract：AIMS:The present study was designed to determine the individual and combined effects of acidified sodium chlorite (ASC, 0.1%, 24 +/- 1 degrees C), cetylpyridinium chloride (CPC, 0.5%, 24 +/- 1 degrees C) and hot water (HW, 93 +/- 1 degrees C) treatments on the survival of Listeria monocytogenes and Staphylococcus aureu...

journal_title：Letters in applied microbiology

authors： Ozdemir H,Koluman A,Yildirim Y

更新日期：2006-08-01 00:00:00

abstract：AIM:The mechanisms of adaptation of Clostridium perfringens to high temperatures are not well understood. In this work, the involvement of extracellular compounds in protection to heat was determined. METHODS AND RESULTS:Cells were grown in fluid thioglycollate medium or chicken broth. When mid-log phase was reached, ...

journal_title：Letters in applied microbiology

authors： Heredia N,Ybarra P,Hernández C,García S

更新日期：2009-01-01 00:00:00

abstract：AIMS:This paper attempts to develop a kinetic model to describe the growth of aerobic granules developed under different operation conditions. METHODS AND RESULTS:A series of experiments were conducted by using four-column sequencing batch reactors to study the formation of aerobic granules under different conditions,...

journal_title：Letters in applied microbiology

authors： Yang SF,Liu QS,Tay JH,Liu Y

更新日期：2004-01-01 00:00:00

abstract：UNLABELLED:The aim of this study was to assess the genetic diversity of Vibrio cholerae isolated from 2012 and 2013 outbreaks in Iran, with regard to their virulence properties. A total of 20 V. cholerae strains were collected from Sistan-Baluchestan province of Iran during 2012 and 2013 outbreaks. Hybridization assays...

journal_title：Letters in applied microbiology

authors： Bakhshi B

更新日期：2016-06-01 00:00:00

abstract：:Staphylococcus aureus is a major agent of bovine mastitis in dairy herds, causing economic losses in dairy industry worldwide. In addition, milk and milk-products contaminated by Staph. aureus can cause harmful human diseases. The aim of this study was to characterize Staph. aureus strains isolated from dairy farms in...

journal_title：Letters in applied microbiology

authors： Ben Said M,Abbassi MS,Bianchini V,Sghaier S,Cremonesi P,Romanò A,Gualdi V,Hassen A,Luini MV

更新日期：2016-12-01 00:00:00

abstract：:The cell surface hydrophobicity of micro-organisms is a characteristic that has been associated with the colonization of mammalian epithelia and with their capacity to induce diseases. Melatonin is a hormone produced by the pineal gland that affects the immune response mechanism. This study investigated, as an express...

journal_title：Letters in applied microbiology

authors： Uberos J,Molina A,Liébana J,Augustin MC,Muñoz A

更新日期：2000-10-01 00:00:00

abstract：UNLABELLED:Faecal indicator bacteria (FIB) and harmful algal blooms (HABs) threaten the health and the economy of coastal communities worldwide. Emerging automated sampling technologies combined with molecular analytical techniques could enable rapid detection of micro-organisms in-situ, thereby improving resource mana...

journal_title：Letters in applied microbiology

authors： Yamahara KM,Demir-Hilton E,Preston CM,Marin R 3rd,Pargett D,Roman B,Jensen S,Birch JM,Boehm AB,Scholin CA

更新日期：2015-08-01 00:00:00

abstract：:A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10(8) cfu ml-1, as found in ...

journal_title：Letters in applied microbiology

authors： Brooks T,Keevil CW

更新日期：1997-03-01 00:00:00

abstract：UNLABELLED:Antibiotic-resistant Salmonella strains were isolated from saladette and red round type tomatoes, and an analysis done of the antibacterial activity of roselle calyx extracts against any of the identified strains. One hundred saladette tomato samples and 100 red round tomato samples were collected from publi...

journal_title：Letters in applied microbiology

authors： Gutiérrez-Alcántara EJ,Rangel-Vargas E,Gómez-Aldapa CA,Falfan-Cortes RN,Rodríguez-Marín ML,Godínez-Oviedo A,Cortes-López H,Castro-Rosas J

更新日期：2016-02-01 00:00:00

abstract：:Heat stable antifungal factor (HSAF) is considered to be a potential biological pesticide due to its broad antifungal activity and novel mode of action. However, few studies have reported on HSAF production during fermentation. Thus, this work was executed to optimize the medium composition to maximize HSAF production...

journal_title：Letters in applied microbiology

authors： Tang B,Zhao YC,Shi XM,Xu HY,Zhao YY,Dai CC,Liu FQ

更新日期：2018-05-01 00:00:00

abstract：AIM:To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture. METHODS AND RESULTS:A Matlab algorithm was developed for the estimation of model parameters....

journal_title：Letters in applied microbiology

authors： Aguilar-Uscanga MG,Garcia-Alvarado Y,Gomez-Rodriguez J,Phister T,Delia ML,Strehaiano P

更新日期：2011-08-01 00:00:00

abstract：:The Crystal Enteric/Nonfermenter (E/NF) identification kit (Becton Dickinson Microbiology Systems, USA) was evaluated using water-derived bacterial isolates and results compared to those obtained by the API 20E system (BioMérieux, UK). Both the E/NF and 20E systems correctly identified 93% of the Enterobacteriaceae re...

journal_title：Letters in applied microbiology

authors： Micklewright IJ,Sartory DP

更新日期：1995-09-01 00:00:00

abstract：:Listonella (Vibrio) anguillarum, an important fish pathogen, is divided into 10 serotypes according to O-antigens present on the outer membrane. However, the biochemical and immunological properties of porin proteins have not been reported. In this study, the antigenicity and N-terminal amino acid sequence of the 35 k...

journal_title：Letters in applied microbiology

authors： Suzuki S,Kuroe K,Yasue K,Kusuda R

更新日期：1996-11-01 00:00:00

abstract：AIMS:To assess the effectiveness of sequential treatments of radish seeds with aqueous chlorine dioxide (ClO(2) ) and dry heat in reducing the number of Escherichia coli O157:H7. METHODS AND RESULTS:Radish seeds containing E. coli O157:H7 at 5.5 log CFU g(-1) were treated with 500 μg ml(-1) ClO(2) for 5 min and subseq...

journal_title：Letters in applied microbiology

authors： Bang J,Kim H,Kim H,Beuchat LR,Kim Y,Ryu JH

更新日期：2011-10-01 00:00:00

abstract：AIMS:To develop and evaluate a new and reliable real-time PCR detection protocol on chromosomal DNA of the contagious plant pathogenic bacterium Erwinia amylovora, the causal agent of fire blight. METHODS AND RESULTS:A Taqman minor-groove-binder real-time PCR assay targeting a hypothetical protein coding gene of Erw. ...

journal_title：Letters in applied microbiology

authors： Gottsberger RA

更新日期：2010-09-01 00:00:00