Improved genetic transformation methods for the model alkaliphile Bacillus halodurans C-125.


AIMS:Bacillus halodurans C-125 is a Gram-positive bacterium that was the first alkaliphilic species to have its genome completely sequenced. Despite its many years as a model for alkaliphily and source of industrially important enzymes, genetic manipulation of B. halodurans C-125 remains difficult, and therefore, we sought to develop a robust method to allow routine transformation of this organism. METHODS AND RESULTS:A plasmid artificial modification system (PAM system, Yasui et al. 2008) for B. halodurans C-125 was created that increases transformation efficiency by 10- to 1000-fold. Also, recovering transformed protoplasts on succinate nutrient agar (SNA) yields faster, more robust colony recovery than on the traditional recovery medium. Combining these two techniques often allows recovery of transformants in as little as 48 h. CONCLUSIONS:Use of the B. halodurans C-125 PAM system and SNA greatly improves the efficiency and speed of protoplast transformation of B. halodurans C-125. SIGNIFICANCE AND IMPACT OF THE STUDY:These techniques allow routine genetic manipulation of B. halodurans C-125, a model alkaliphilic bacterium with important industrial properties.


Lett Appl Microbiol


Wallace JG,Breaker RR




Has Abstract


2011-04-01 00:00:00












  • An in-vitro study on a novel six-phage cocktail against multi-drug resistant-ESBL Shigella in aquatic environment.

    abstract::Shigella spp. are water-borne pathogens responsible for mild to severe cases bacilli dysentery all around the world known as Shigellosis. The progressively increasing of antibiotic resistance among Shigella calls for developing and establishing novel alternative therapeutic methods. The present study aimed to evaluate...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Shahin K,Zhang L,Bao H,Hedayatkhah A,Soleimani-Delfan A,Komijani M,He T,Barazandeh M,Mansoorianfar M,Bouzari M,Wang R

    更新日期:2020-10-18 00:00:00

  • Detection and discrimination of four Aspergillus section Nigri species by PCR.

    abstract::Species of Aspergillus section Nigri are not easily distinguished by traditional morphological techniques, and typically are identified by DNA sequencing methods. We developed four PCR primers to distinguish between Aspergillus niger, Aspergillus welwitschiae, Aspergillus carbonarius and Aspergillus tubingensis, based...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Palumbo JD,O'Keeffe TL

    更新日期:2015-02-01 00:00:00

  • Rapid RAPD analysis for distinguishing Listeria species and Listeria monocytogenes serotypes using a capillary air thermal cycler.

    abstract::Using a novel capillary thermal cycler, randomly amplified polymorphic DNA (RAPD) generated DNA fingerprints were obtained in 3 h. The RAPD profiles were produced using a random 10-mer primer (5'-ACCGCCTGCT-3') which discriminated between different Listeria spp. Unique fragment profiles of Listeria monocytogenes serot...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Black SF,Gray DI,Fenlon DR,Kroll RG

    更新日期:1995-03-01 00:00:00

  • Loop-mediated isothermal amplification method for rapid detection of Vibrio alginolyticus, the causative agent of vibriosis in mariculture fish.

    abstract:AIMS:The purpose of this study was to develop a loop-mediated isothermal amplification (LAMP) method for the rapid, sensitive and simple detection of Vibrio alginolyticus in mariculture fish. METHODS AND RESULTS:LAMP primers were designed by targeting the gyrB gene. With Bst DNA polymerase, the target DNA can be clear...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Cai SH,Lu YS,Wu ZH,Jian JC,Wang B,Huang YC

    更新日期:2010-05-01 00:00:00

  • Application of ARISA to assess the influence of salt content and cation type on microbiological diversity of Cheddar cheese.

    abstract:UNLABELLED:The structure and dynamics of microbial populations play a significant role during cheese manufacture and ripening. Therefore, fast and accurate methods for identification and characterization of the microbial populations are of fundamental importance to the cheese industry. In this study, we investigate the...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Porcellato D,Brighton C,McMahon DJ,Oberg CJ,Lefevre M,Broadbent JR,Steele JL

    更新日期:2014-08-01 00:00:00

  • Pseudomonas fluorescens and closely-related fluorescent pseudomonads as biocontrol agents of soil-borne phytopathogens.

    abstract::Many strains of Pseudomonas fluorescens show potential for biological control of phytopathogens especially root pathogens. In taxonomic terms, several of them are indeed P. fluorescens sensu stricto, while others belong in fact to neighbouring species of the 'P. fluorescens' complex or to ill-defined related species w...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Couillerot O,Prigent-Combaret C,Caballero-Mellado J,Moënne-Loccoz Y

    更新日期:2009-05-01 00:00:00

  • Antibiotic susceptibility of potentially probiotic Bifidobacterium isolates from the human gastrointestinal tract.

    abstract::Sixteen Bifidobacterium isolates from the human gastrointestinal tract were assayed for susceptibility to 44 antibiotics by soft agar overlay disc diffusion on TPY agar. Five isolates (3/7 B. bifidum and 2/3 B. breve) exhibited atypical antibiotic susceptibility profiles. Poor growth in the agar overlay accounted for ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Charteris WP,Kelly PM,Morelli L,Collins JK

    更新日期:1998-05-01 00:00:00

  • Antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20.

    abstract:AIMS:The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. METHODS AND RESULTS:Antimicrobial and ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Gudiña EJ,Rocha V,Teixeira JA,Rodrigues LR

    更新日期:2010-04-01 00:00:00

  • Hydrolytic properties of crude alpha-L-rhamnosidases produced by several wild strains of mesophilic fungi.

    abstract:AIMS:Observation of the dependence of alpha-L-rhamnosidase activity on pH and temperature and the capability to hydrolyse concentrated naringin solutions and hesperidin suspensions of enzyme complexes produced by several fungi. METHODS AND RESULTS:The enzymes were produced by several wild strains of mesophilic fungi g...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Scaroni E,Cuevas C,Carrillo L,Ellenrieder G

    更新日期:2002-01-01 00:00:00

  • Survival of Salmonella enteritidis Pt4 and Salm. typhimurium Swindon in aerosols.

    abstract::Small particle aerosols of plate-grown Salmonella enteritidis and Salm. typhimurium were generated and maintained within a rotating drum at 75% relative humidity and 24 degrees C for 2 h. Plate-grown organisms were found to be more aerosol-stable than broth-grown organisms. Differences were observed between the two sp...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: McDermid AS,Lever MS

    更新日期:1996-08-01 00:00:00

  • A heat inducible tyrosinase with distinct properties from Bacillus thuringiensis.

    abstract:AIMS:The objective of this work was to purify the tyrosinase from Bacillusthuringiensis subsp. kurstaki (Bt) (CCTCC AB 90010) and study its enzymatic properties. METHODS AND RESULTS:A 'one-step' purification method was used in this work, which was an easy, high-yield purification method. Tyrosinase activity of this pu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Liu N,Zhang T,Wang YJ,Huang YP,Ou JH,Shen P

    更新日期:2004-01-01 00:00:00

  • In vitro growth control of selected pathogens by Lactobacillus acidophilus- and Lactobacillus casei-fermented milk.

    abstract:AIMS:Food-borne pathogen inhibition was tested in the presence of a mixture of Lactobacillus acidophilus and Lactobacillus casei during fermentation under controlled pH conditions. METHODS AND RESULTS:The growth of Escherichia coli O157:H7, Salmonella serotype Typhimurium, Staphylococcus aureus, Listeria innocua, Ente...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Millette M,Luquet FM,Lacroix M

    更新日期:2007-03-01 00:00:00

  • Intranasal administration of Lactobacillus rhamnosus GG protects mice from H1N1 influenza virus infection by regulating respiratory immune responses.

    abstract:AIMS:To investigate whether intranasal Lactobacillus administration protects host animals from influenza virus (IFV) infection by enhancing respiratory immune responses in a mouse model. METHODS AND RESULTS:After 3 days of intranasal exposure to Lactobacillus rhamnosus GG (LGG), BALB/c mice were infected with IFV A/PR...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Harata G,He F,Hiruta N,Kawase M,Kubota A,Hiramatsu M,Yausi H

    更新日期:2010-06-01 00:00:00

  • Quantification of the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 at lethal temperatures.

    abstract:AIM:To quantify the influence of trimethylamine-N-oxide (TMAO) on the heat resistance of Escherichia coli K12 MG1655 cells at static temperatures. METHODS AND RESULTS:Stationary-phase E. coli cells were inactivated at 52, 54 and 58°C. The heat resistance is described as reduction in the inactivation rate, k(max) , and...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Velliou EG,Van Derlinden E,Cappuyns AM,Aerts D,Nikolaidou E,Geeraerd AH,Devlieghere F,Van Impe JF

    更新日期:2011-02-01 00:00:00

  • Evaluation of an alternative method for the enumeration and confirmation of Clostridium perfringens from treated and untreated sewages.

    abstract:AIMS:Clostridium perfringens is recommended as a suitable indicator bacterium for human enteric viruses, Giardia cysts and Cryptosporidium oocysts in finished water and in the assessment and evaluation of water treatment. Several agars and confirmation procedures were evaluated in parallel with the Australian/New Zeala...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Wohlsen T,Bayliss J,Gray B,Bates J,Katouli M

    更新日期:2006-05-01 00:00:00

  • Detection and characterization of Clostridium difficile in retail chicken.

    abstract:AIMS:This study was designed to evaluate the prevalence of Clostridium difficile contamination of retail chicken. METHODS AND RESULTS:Chicken legs, thighs and wings were purchased using a standardized method from retail outlets across Ontario, Canada. Selective culture was used for qualitative and quantitative detecti...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Weese JS,Reid-Smith RJ,Avery BP,Rousseau J

    更新日期:2010-04-01 00:00:00

  • Effect of penicillin G on the electroporation of Rhodococcus rhodochrous CF222.

    abstract::Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different tr...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Sunairi M,Iwabuchi N,Murakami K,Watanabe F,Ogawa Y,Murooka H,Nakajima M

    更新日期:1996-01-01 00:00:00

  • Phosphate promotes uranium (VI) adsorption in Staphylococcus aureus LZ-01.

    abstract:UNLABELLED:Staphylococcus aureus LZ-01 was isolated from the Yellow River upstream from Lanzhou which can resist and reduce chromium (VI) to chromium (III). In this study, strain LZ-01's uranium (VI) resistance and adsorption abilities were investigated. Our results showed that it can resist 2 mmol l(-1) U(VI) and adso...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Zou L,Chen Z,Zhang X,Liu P,Li X

    更新日期:2014-11-01 00:00:00

  • Rapid construction of Campylobacter jejuni deletion mutants.

    abstract:AIMS:To develop a novel method for rapid construction of Campylobacter jejuni deletion mutants. METHODS AND RESULTS:We used overlapping extension PCR protocol to amplify a target sequence region of Camp. jejuni genomic DNA in which an internal fragment, Cj0618 coding sequence, was replaced by a chloramphenicol resista...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Hansen CR,Khatiwara A,Ziprin R,Kwon YM

    更新日期:2007-12-01 00:00:00

  • Characterization of amino acid transport in the dairy strain Leuconostoc mesenteroides subsp. mesenteroides CNRZ 1273.

    abstract:AIMS:To identify and characterize amino acid transport in Leuconostoc mesenteroides. METHODS AND RESULTS:The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transp...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Gendrot F,Foucaud-Scheunemann C,Ferchichi M,Hemme D

    更新日期:2002-01-01 00:00:00

  • A preliminary assessment of Bacillus anthracis spore inactivation using an electrochemically activated solution (ECASOL).

    abstract:AIM:To evaluate the efficacy of electrochemically activated solution (ECASOL) in decontaminating Bacillus anthracis Ames and Vollum 1B spores, with and without changing the source water hardness and final ECASOL pH. METHODS AND RESULTS:Five different ECASOL formulations were generated, in which the source water hardne...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Rogers JV,Ducatte GR,Choi YW,Early PC

    更新日期:2006-11-01 00:00:00

  • Assessment of alcohol percentage test for fungal surface hydrophobicity measurement.

    abstract:AIM:To determine whether assessing the penetration of solutions with different concentrations of ethanol (alcohol percentage test: APT) on fungal surfaces is effective in characterization of hydrophobicity on fungal surfaces. METHODS AND RESULTS:APT and contact angle (CA) measurements were conducted on nine hydrophobi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Chau HW,Goh YK,Si BC,Vujanovic V

    更新日期:2010-03-01 00:00:00

  • Kinetics and effects of trace elements and electron complexes on 2-keto-4-methylthiobutyric acid-dependent biosynthesis of ethylene in soil.

    abstract:AIMS:2-Keto-4-methylthiobutyric acid (KMBA) is an established intermediate in microbial biosynthesis of ethylene from methionine. This study demonstrates the kinetics and effects of trace elements and electron complexes on substrate (KMBA)-derived C2H4 biosynthesis in soil. METHODS AND RESULTS:We have previously repor...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Arshad M,Nazli ZH,Khalid A,Zahir ZA

    更新日期:2004-01-01 00:00:00

  • Blue light (470 nm) effectively inhibits bacterial and fungal growth.

    abstract:UNLABELLED:Blue light (470 nm) LED antimicrobial properties were studied alone against bacteria and with or without the food grade photosensitizer, erythrosine (ERY) against filamentous fungi. Leuconostoc mesenteroides (LM), Bacillus atrophaeus (BA) or Pseudomonas aeruginosa (PA) aliquots were exposed on nutrient agar ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: De Lucca AJ,Carter-Wientjes C,Williams KA,Bhatnagar D

    更新日期:2012-12-01 00:00:00

  • Enhanced poly-beta-hydroxybutyrate accumulation in a unicellular cyanobacterium, Synechocystis sp. PCC 6803.

    abstract:AIM:To stimulate poly-beta-hydroxybutyrate (PHB) accumulation in Synechocystis sp. PCC 6803 by manipulating culture conditions. METHODS AND RESULTS:Stationary phase cultures of Synechocystis sp. PCC 6803 were subjected to N- and P-deficiency, chemoheterotrophy and limitations of gas-exchange. Enhanced PHB accumulation...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Panda B,Mallick N

    更新日期:2007-02-01 00:00:00

  • Comparison of three immunological methods for detecting staphylococcal enterotoxins from food.

    abstract:AIMS:Immunologically based assays for the detection of staphylococcal enterotoxins are numerous. These techniques include radio immunosorbent assays and enzyme-linked immunosorbent assays (ELISA), some of which are available as commercial kits. The purpose of this study was to compare the performances of three commerci...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Vernozy-Rozand C,Mazuy-Cruchaudet C,Bavai C,Richard Y

    更新日期:2004-01-01 00:00:00

  • Modelling the growth and ethanol production of Brettanomyces bruxellensis at different glucose concentrations.

    abstract:AIM:To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture. METHODS AND RESULTS:A Matlab algorithm was developed for the estimation of model parameters....

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Aguilar-Uscanga MG,Garcia-Alvarado Y,Gomez-Rodriguez J,Phister T,Delia ML,Strehaiano P

    更新日期:2011-08-01 00:00:00

  • Lactobacilli prevent hydroxy radical production and inhibit Escherichia coli and Enterococcus growth in system mimicking colon fermentation.

    abstract:AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Sun J,Hu XL,Le GW,Shi YH

    更新日期:2010-03-01 00:00:00

  • Influence of sodium chloride on the beta-glucuronidase activity of Clostridium perfringens and Escherichia coli.

    abstract::While the beta-glucuronidase activity of intact cells of Clostridium perfringens was higher in 0.95% sodium chloride (NaCl) than that in 0, 0.1 or 0.5%, that of Escherichia coli was higher in 0.1% NaCl than that in 0, 0.5 or 0.95% NaCl in 0.1 mol l-1 KH2PO4. However, the enzyme activity of both species of intact cells...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Fujisawa T,Aikawa K,Takahashi T,Yamai S

    更新日期:2000-09-01 00:00:00

  • Anti-listerial activity of bacteriocin-producing Lactobacillus curvatus CWBI-B28 and Lactobacillus sakei CWBI-B1365 on raw beef and poultry meat.

    abstract:AIM:The study aimed to evaluate the effect of the bacteriocins produced by Lactobacillus sakei CWBI-B1365 and Lactobacillus curvatus CWBI-B28 on the growth and survival of Listeria monocytogenes in raw beef and poultry meat. METHODS AND RESULTS:The sakacin P and sakacin G structural genes were identified in Lact. curv...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章


    authors: Dortu C,Huch M,Holzapfel WH,Franz CM,Thonart P

    更新日期:2008-12-01 00:00:00