Abstract:
AIMS:The antigen 85 complex (Ag85) from Mycobacterium tuberculosis consists of three abundantly secreted proteins (FbpA, FbpB and FbpC2) which play a key role in the pathogenesis of tuberculosis and also exhibit cell wall mycolyltransferase activity. A related protein with similarity to the Ag85 complex was recently annotated in the M. tuberculosis genome as FbpC1. An investigation was carried out to determine whether FbpC1 may also possess mycolyltransferase activity, a characteristic feature of the Ag85 complex. METHODS AND RESULTS:Heterologous expression of FbpA, FbpC1 and FbpC2 was performed in Escherichia coli. Recombinant proteins were purified under non-denaturating conditions and used in an in vitro mycolyltransferase assay. CONCLUSIONS:In contrast to FbpA and FbpC2, recombinant FbpC1 did not possess in vitro mycolyltransferase activity and was not recognized by two monoclonal antibodies to the native Ag85. SIGNIFICANCE AND IMPACT OF THE STUDY:Mycolyltransferase activity is restricted to FbpA, FbpbB and FbpC2 only; the actual function of FbpC1 remains to be established.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Kremer L,Maughan WN,Wilson RA,Dover LG,Besra GSdoi
10.1046/j.1472-765x.2002.01091.xsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
233-7issue
4eissn
0266-8254issn
1472-765Xpii
1091journal_volume
34pub_type
杂志文章abstract:AIMS:Breast milk has been described as a source of bacteria influencing the development of the infant gut microbiota. Up to the present, few studies have been focused on the application of culture-independent techniques to study bacterial diversity in breast milk. In this context, the aim of this study was to character...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02567.x
更新日期:2009-05-01 00:00:00
abstract::A monoclonal antibody (mAb) has been produced to aflatoxin B1 (AF B1) after successful immunization of mice and fusion of sensitized spleen cells with myeloma cancer cells. The mice were immunized with AF B1-oxime-protein conjugate. Positive mAbs were screened using an indirect ELISA specific for AF B1. The selected m...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1990.tb00106.x
更新日期:1990-04-01 00:00:00
abstract::Species of Aspergillus section Nigri are not easily distinguished by traditional morphological techniques, and typically are identified by DNA sequencing methods. We developed four PCR primers to distinguish between Aspergillus niger, Aspergillus welwitschiae, Aspergillus carbonarius and Aspergillus tubingensis, based...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12358
更新日期:2015-02-01 00:00:00
abstract:AIMS:The current study examined the antimicrobial susceptibility of 86 Listeria spp. isolated from processed bison carcasses. MATERIALS AND METHODS:Susceptibility to 25 antimicrobial agents was determined using E-test and National Antimicrobial Resistance Monitoring System (NARMS) panels. Most Listeria isolates (88-98...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.02027.x
更新日期:2007-01-01 00:00:00
abstract::A set of Escherichia coli freshwater isolates was chosen to compare the effectiveness of denaturing gradient gel electrophoresis (DGGE) vs temporal temperature gradient gel electrophoresis (TTGE) for separating homologous amplicons from the respective uidA region differing in one to seven single base substitutions. Bo...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00742.x
更新日期:2000-06-01 00:00:00
abstract:AIMS:To establish species-specific PCR and DNA probe methods for Streptococcus salivarius and to clarify the distribution of dextranase in oral isolates of Strep. salivarius. METHODS AND RESULTS:A pair of PCR primers and a DNA probe were designed based on the nucleotide sequence of the dextranase gene of Strep. saliva...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00928.x
更新日期:2001-06-01 00:00:00
abstract::Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13010
更新日期:2018-09-01 00:00:00
abstract:AIMS:To investigate the diversity and dissemination of tetracycline resistance genes in isolates from estuarine waters. METHODS AND RESULTS:Forty-two out of 164 multi-resistant isolates previously obtained were resistant or less-susceptible to tetracycline, as evaluated by the disc diffusion method. Minimal inhibitory...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02452.x
更新日期:2008-12-01 00:00:00
abstract::Novel findings in microbiology question the long-standing paradigm that a healthy pregnancy implies a sterile uterus. It now seems that the placenta is frequently colonized with bacteria, and a placental microbiome has been identified. Recent literature findings are summarized here, and an attempt is made to separate ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章,评审
doi:10.1111/lam.12334
更新日期:2014-12-01 00:00:00
abstract::Salmonella enteritidis-inoculated poultry sausages were pressurized at 500 MPa by combining different times (10 and 30 min) and temperatures (50, 60 and 70 degrees C) or heat treated with the same temperature-time combinations and a standard cooking (75 degrees C for 30 min). Counts of Salm. enteritidis and mesophilic...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2000.00829.x
更新日期:2000-11-01 00:00:00
abstract::The neurotoxins produced by Clostridium botulinum are amongst the most potent known to man. Toxin production is detected by a mouse bioassay, which requires several days for a result and is not acceptable for routine use unless there is a high level of suspicion. The Rapid ID32 A kit produced by bioMerieux gives an id...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1998.00283.x
更新日期:1998-01-01 00:00:00
abstract:AIMS:To develop and evaluate a new and reliable real-time PCR detection protocol on chromosomal DNA of the contagious plant pathogenic bacterium Erwinia amylovora, the causal agent of fire blight. METHODS AND RESULTS:A Taqman minor-groove-binder real-time PCR assay targeting a hypothetical protein coding gene of Erw. ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02892.x
更新日期:2010-09-01 00:00:00
abstract:AIMS:To explore the effect of Lactobacillus on redox state of colon chyme. METHODS AND RESULTS:Nine Lactobacillus strains were studied for the inhibition of lipid peroxide formation in Fe(2+)/ascorbate system and for their ability to chelate 'free' ferrous ion. The result shows both properties were strain specific and...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02786.x
更新日期:2010-03-01 00:00:00
abstract:AIMS:The aim of this study was to investigate the role of divercin V41 in inhibition and prevention of Listeria monocytogenes. METHODS AND RESULTS:Carnobacterium divergens V41 deficient in bacteriocin production was isolated and characterized by enzyme-liked immunosorbent assay, multiplex polymerase chain reaction and...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01310.x
更新日期:2003-01-01 00:00:00
abstract:UNLABELLED:The mechanisms and organisms involved in the natural formation of volatile organohalogen compounds (VOX) are largely unknown. We provide evidence that the common and widespread soil bacterium Sinorhizobium meliloti strain 1021 is capable of producing up to 3338·6 ± 327·8 ng l(-1) headspace volume of chlorofo...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12462
更新日期:2015-10-01 00:00:00
abstract:AIMS:To improve the production of sweet-tasting protein brazzein in Lactococcus lactis using controlled fermentation conditions. METHODS AND RESULTS:The nisin-controlled expression system was used for brazzein expression. The concentration of nisin for induction and the optical density (OD) at induction were therefore...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02297.x
更新日期:2008-02-01 00:00:00
abstract:AIMS:To isolate gamma-hexachlorocyclohexane (gamma-HCH)-degrading bacteria from a single field and to examine their genetic diversity. METHODS AND RESULTS:Gamma-HCH-degrading bacteria were screened from a long-term experimental field in which gamma-HCH has been continuously applied to, and a gamma-HCH-degrading sphing...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2009.02691.x
更新日期:2009-10-01 00:00:00
abstract:UNLABELLED:AIMIS: Draft WHO guidance is likely to suggest a calculated 95th percentile method, rather than percentage exceedence, in assessing compliance of bathing waters with microbiological standards. This study set out to determine whether this was an appropriate development. METHODS AND RESULTS:A series of Monte ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01081.x
更新日期:2002-01-01 00:00:00
abstract::PCR primers were selected from the flagellin gene sequences flaA and flaB of Campylobacter coli to amplify DNA from Camp. jejuni and Camp. coli. When the PCR products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of Camp. jejuni a...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1996.tb00209.x
更新日期:1996-11-01 00:00:00
abstract::An efficient method is described for the purification of Ti plasmid DNA from Agrobacterium. The procedure is based on the relative binding capacity of ethidium bromide to supercoiled plasmid DNA and linear DNA and on the high solubility of ethidium bromide in phenol. Following treatment with ethidium bromide, more tha...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765x.1993.tb01415.x
更新日期:1993-05-01 00:00:00
abstract:AIMS:To find a supplemental ingredient that can be added to routinely used growth media to increase conidial production and decrease aflatoxin biosynthesis in small sclerotial (S strain) isolates of Aspergillus flavus. METHODS AND RESULTS:Molasses was added to three commonly used culture media: coconut agar (CAM), pot...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2006.02056.x
更新日期:2007-02-01 00:00:00
abstract::A strain named as Pseudomonas aeruginosa 2016NX1, which could produce phenazine and cereusitin, was isolated from the root of Millettia specisoa. Phenazines were extracted, isolated and purified by chloroform, thin-layer chromatography, column chromatography and high-performance liquid chromatography. Then the purifie...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13332
更新日期:2020-09-01 00:00:00
abstract:AIMS:To test the suitability of cheese whey powder (CWP) solution for ethanol fermentation and to compare performances of different Kluyveromyces marxianus strains for ethanol fermentation from CWP solution. METHODS AND RESULTS:Batch ethanol fermentation of cheese whey (CW), CWP and lactose solutions with the same ini...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02132.x
更新日期:2007-06-01 00:00:00
abstract:AIMS:To investigate the genetics of dimethoate degradation in Pseudomonas aeruginosa MCMB-427. METHODS AND RESULTS:Pseudomonas aeruginosa MCMB-427 demonstrated the ability to degrade dimethoate, a synthetic organophosphate insecticide. Total DNA preparation of MCMB-427 revealed the presence of a 6.6 kbp plasmid (desig...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00995.x
更新日期:2001-10-01 00:00:00
abstract:AIM:To examine whether isolates of the entomopathogenic fungus Beauveria bassiana are more closely associated to their summer hosts compared with overwintering hosts, with recently developed molecular tools based on mitochondrial regions. METHODS AND RESULTS:Primers for the traditional ITS1-5.8S-ITS2 region and two mi...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2008.02331.x
更新日期:2008-03-01 00:00:00
abstract:AIMS:To investigate interactions, if any, between temperature, ferric ammonium citrate and glycine betaine on the growth of Listeria monocytogenes in modified Pine's medium (Pine et al. 1989). METHODS AND RESULTS:Modified Pine's medium containing 0, 0.044, 0.088 or 0.176 g l(-1) ferric ammonium citrate, and 0 or 1 mM ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01237.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:The objective of this study was to subtype Arcobacter butzleri isolates using RAPD-PCR. METHODS AND RESULTS:Thirty-five A. butzleri isolates obtained from chicken carcasses were examined. PCR-mediated DNA fingerprinting technique with primers of the variable sequence motifs was used to detect polymorphism within ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01152.x
更新日期:2002-01-01 00:00:00
abstract:AIMS:To identify and characterize amino acid transport in Leuconostoc mesenteroides. METHODS AND RESULTS:The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transp...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01191.x
更新日期:2002-01-01 00:00:00
abstract::The bacterial diversity and community structure involved in Chinese sauerkraut is one of the most important factors shaping the final characteristics of traditional foods. In this research, Lactobacillus casei 11MZ-5-1 was applied in Chinese sauerkraut fermentation as a starter culture. Illumina Miseq sequencing analy...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12824
更新日期:2018-01-01 00:00:00
abstract::The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n = 4), V. alginolyticus (n = 11), V. diabolicus (n = 14) and V. harveyi (n = 3) were ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13232
更新日期:2020-07-01 00:00:00