Abstract:
AIMS:The risk of hepatitis C virus infection in hospital environments can be assessed not only by studying epidemiological data and work practices, but also by the detection of these viruses (or indicators thereof) in health-care settings, on instruments etc. METHODS:Since standardized techniques specific to this end do not exist, this study was undertaken to apply methods currently used on clinical samples to the assessment of environmental HCV risk, either through direct detection of the virus (RT-PCR), or by probing for haemoglobin as a potential indicator of blood contamination. The tested techniques were applied in a trial environmental monitoring programme undertaken in various hospital laboratories and clinics, during which total bacterial count determinations were performed in parallel with haemoglobin and hepatitis C virus detection. SIGNIFICANCE AND IMPACT OF THE STUDY:The data indicate that the applied methods are of value in detecting low levels of contamination in a hospital environment.
journal_name
Lett Appl Microbioljournal_title
Letters in applied microbiologyauthors
Carducci A,Verani M,Casini B,Giuntini A,Mazzoni F,Rovini E,Passaglia A,Giusti L,Valenza A,Lombardi Rdoi
10.1046/j.1472-765x.2002.01066.xsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
189-93issue
3eissn
0266-8254issn
1472-765Xpii
1066journal_volume
34pub_type
杂志文章abstract::Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different tr...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
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journal_title:Letters in applied microbiology
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abstract:AIMS:Strains of Clostridium butyricum have been increasingly used as probiotics for both animals and humans. The aim of this study was to develop a growth medium for cultivating C. butyricum ZJUCB using a statistical methodology. METHODS AND RESULTS:Response surface methodology (RSM) was used to evaluate the effects o...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2004.01595.x
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doi:10.1111/j.1472-765X.2004.01602.x
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2005.01710.x
更新日期:2005-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2003.01370.x
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pub_type: 杂志文章
doi:10.1111/lam.12873
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2001.00977.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01191.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.00378.x
更新日期:1997-03-01 00:00:00
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journal_title:Letters in applied microbiology
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doi:10.1111/lam.12417
更新日期:2015-06-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2007.02258.x
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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journal_title:Letters in applied microbiology
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doi:10.1111/j.1472-765X.2008.02331.x
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journal_title:Letters in applied microbiology
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Letters in applied microbiology
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2008-03-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12310
更新日期:2014-11-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.13344
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.1997.00207.x
更新日期:1997-10-01 00:00:00
abstract::This study was undertaken to detect the occurrence of beta-lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n = 92) and backyard (n = 110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
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更新日期:2019-08-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2011.03081.x
更新日期:2011-08-01 00:00:00
abstract:AIMS:To develop and evaluate a new and reliable real-time PCR detection protocol on chromosomal DNA of the contagious plant pathogenic bacterium Erwinia amylovora, the causal agent of fire blight. METHODS AND RESULTS:A Taqman minor-groove-binder real-time PCR assay targeting a hypothetical protein coding gene of Erw. ...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/j.1472-765X.2010.02892.x
更新日期:2010-09-01 00:00:00
abstract::Fresh-cut produce is at greater risk of Salmonella contamination. Detection and early warning systems play an important role in reducing the dissemination of contaminated products. One-step Reverse Transcription Polymerase Chain Reaction (RT-qPCR) targeting Salmonella tmRNA with or without a 6-h enrichment was evaluat...
journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12871
更新日期:2018-05-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1046/j.1472-765x.2002.01152.x
更新日期:2002-01-01 00:00:00
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journal_title:Letters in applied microbiology
pub_type: 杂志文章
doi:10.1111/lam.12850
更新日期:2018-04-01 00:00:00