Detection and potential indicators of the presence of hepatitis C virus on surfaces in hospital settings.

Abstract:

AIMS:The risk of hepatitis C virus infection in hospital environments can be assessed not only by studying epidemiological data and work practices, but also by the detection of these viruses (or indicators thereof) in health-care settings, on instruments etc. METHODS:Since standardized techniques specific to this end do not exist, this study was undertaken to apply methods currently used on clinical samples to the assessment of environmental HCV risk, either through direct detection of the virus (RT-PCR), or by probing for haemoglobin as a potential indicator of blood contamination. The tested techniques were applied in a trial environmental monitoring programme undertaken in various hospital laboratories and clinics, during which total bacterial count determinations were performed in parallel with haemoglobin and hepatitis C virus detection. SIGNIFICANCE AND IMPACT OF THE STUDY:The data indicate that the applied methods are of value in detecting low levels of contamination in a hospital environment.

journal_name

Lett Appl Microbiol

authors

Carducci A,Verani M,Casini B,Giuntini A,Mazzoni F,Rovini E,Passaglia A,Giusti L,Valenza A,Lombardi R

doi

10.1046/j.1472-765x.2002.01066.x

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

189-93

issue

3

eissn

0266-8254

issn

1472-765X

pii

1066

journal_volume

34

pub_type

杂志文章
  • Effect of penicillin G on the electroporation of Rhodococcus rhodochrous CF222.

    abstract::Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different tr...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1996.tb01110.x

    authors: Sunairi M,Iwabuchi N,Murakami K,Watanabe F,Ogawa Y,Murooka H,Nakajima M

    更新日期:1996-01-01 00:00:00

  • High organic loading influences the physical characteristics of aerobic sludge granules.

    abstract:AIMS:The effect of high organic loading rate (OLR) on the physical characteristics of aerobic granules was studied. METHODS AND RESULTS:Two column-type sequential aerobic sludge blanket reactors were fed with either glucose or acetate as the main carbon source, and the OLR was gradually raised from 6 to 9, 12 and 15 k...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01108.x

    authors: Moy BY,Tay JH,Toh SK,Liu Y,Tay ST

    更新日期:2002-01-01 00:00:00

  • FISH analysis of Lactobacillus biofilms in the gastrointestinal tract of different hosts.

    abstract:AIMS:To investigate the spatial organization of endogenous and exogenously applied Lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts. METHODS AND RESULTS:Samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a Lactob...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02994.x

    authors: Lebeer S,Verhoeven TL,Claes IJ,De Hertogh G,Vermeire S,Buyse J,Van Immerseel F,Vanderleyden J,De Keersmaecker SC

    更新日期:2011-03-01 00:00:00

  • Evaluation of sample preparation methods for MALDI-TOF MS identification of highly dangerous bacteria.

    abstract:AIMS:To propose a universal workflow of sample preparation method for the identification of highly pathogenic bacteria by MALDI-TOF MS. METHODS AND RESULTS:Fifteen bacterial species, including highly virulent Gram-positive (Bacillus anthracis and Clostridium botulinum) and Gram-negative bacteria (Brucella melitensis, ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2012.03255.x

    authors: Drevinek M,Dresler J,Klimentova J,Pisa L,Hubalek M

    更新日期:2012-07-01 00:00:00

  • Response surface methodology for optimizing the fermentation medium of Clostridium butyricum.

    abstract:AIMS:Strains of Clostridium butyricum have been increasingly used as probiotics for both animals and humans. The aim of this study was to develop a growth medium for cultivating C. butyricum ZJUCB using a statistical methodology. METHODS AND RESULTS:Response surface methodology (RSM) was used to evaluate the effects o...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2004.01595.x

    authors: He GQ,Kong Q,Ding LX

    更新日期:2004-01-01 00:00:00

  • Comparison of three immunological methods for detecting staphylococcal enterotoxins from food.

    abstract:AIMS:Immunologically based assays for the detection of staphylococcal enterotoxins are numerous. These techniques include radio immunosorbent assays and enzyme-linked immunosorbent assays (ELISA), some of which are available as commercial kits. The purpose of this study was to compare the performances of three commerci...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2004.01602.x

    authors: Vernozy-Rozand C,Mazuy-Cruchaudet C,Bavai C,Richard Y

    更新日期:2004-01-01 00:00:00

  • Comparison of sample collection methods for the PCR detection of oral anaerobic pathogens.

    abstract:AIMS:To provide evidence that DNA-PCR diagnostics of oral pathogens based on standard sample collection by paper point insertion from the depth of the periodontal pocket can be replaced by a novel non-invasive collection method based on swab technique from the gingiva. METHODS AND RESULTS:In this study we compared the...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01269.x

    authors: Smola SF,Rettenberger G,Simmet T,Burysek L

    更新日期:2003-01-01 00:00:00

  • Antimicrobial resistance of Salmonella spp. from pigs at slaughter in Spain in 1993 and 2001.

    abstract:AIM:To compare the incidence of antimicrobial resistance among Salmonella serotypes isolated in a pig slaughterhouse in Zaragoza (Spain) during 1993 and 2001. METHODS AND RESULTS:A total of 168 isolates representing 10 serotypes were examined by disc diffusion method using 17 antibiotics. Data showed that the majority...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2005.01710.x

    authors: Agustín AI,Carramiñana JJ,Rota C,Herrera A

    更新日期:2005-01-01 00:00:00

  • Isolation and characteristics of lactic acid bacteria from koshu vineyards in Japan.

    abstract:AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in the vineyards where koshu grapes, a primary wine grape cultivar in Japan, are grown. METHODS AND RESULTS:Sixty samples, including leaves, undamaged grape berries and soil under damaged berries, were collected at four koshu vineyards in Yamanashi ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02398.x

    authors: Yanagida F,Srionnual S,Chen YS

    更新日期:2008-08-01 00:00:00

  • Flurbiprofen, a unique non-steroidal anti-inflammatory drug with antimicrobial activity against Trichophyton, Microsporum and Epidermophyton species.

    abstract:AIMS:The objective of this study was to determine the antifungal activity of flurbiprofen against dermatophytes like Trichophyton, Microsporum and Epidermophyton species. METHODS AND RESULTS:Susceptibility tests were performed against dermatophytes like Trichophyton, Microsporum and Epidermophyton species by the micro...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01370.x

    authors: Chowdhury B,Adak M,Bose SK

    更新日期:2003-01-01 00:00:00

  • Virulence profile of Escherichia coli O157 strains isolated from surface water in cattle breeding areas.

    abstract::Shiga toxin-producing Escherichia coli (STEC) O157:H7 is a worldwide concern. Cattle are their main reservoir and may contaminate watercourses through manure. We characterized a collection of 38 STEC O157:H7 strains isolated from surface water in feedlots areas (puddles inside pens formed after the rainfall or by spil...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12873

    authors: Tanaro JD,Pianciola LA,D'Astek BA,Piaggio MC,Mazzeo ML,Zolezzi G,Rivas M

    更新日期:2018-06-01 00:00:00

  • Utilization of chicory roots for microbial endoinulinase production.

    abstract:AIMS:The optimal culture conditions for endoinulinase production using chicory roots were studied in shake-flask culture. METHODS AND RESULTS:Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml(-1)) than with Pseudomonas sp. (3 U ml(-1)). Optimized culture conditions of Xanthomonas sp. for endoinu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00977.x

    authors: Park JP,Yun JW

    更新日期:2001-09-01 00:00:00

  • Characterization of amino acid transport in the dairy strain Leuconostoc mesenteroides subsp. mesenteroides CNRZ 1273.

    abstract:AIMS:To identify and characterize amino acid transport in Leuconostoc mesenteroides. METHODS AND RESULTS:The transport of labelled amino acids was measured in whole cells of Leuc. mesenteroides CNRZ 1273. Systems were operative under physiological conditions of growth, energy dependent and differed from peptide transp...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01191.x

    authors: Gendrot F,Foucaud-Scheunemann C,Ferchichi M,Hemme D

    更新日期:2002-01-01 00:00:00

  • A simple artificial urine for the growth of urinary pathogens.

    abstract::A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10(8) cfu ml-1, as found in ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00378.x

    authors: Brooks T,Keevil CW

    更新日期:1997-03-01 00:00:00

  • Erwinia amylovora pyrC mutant causes fire blight despite pyrimidine auxotrophy.

    abstract::Erwinia amylovora bacteria cause fire blight disease, which affects apple and pear production worldwide. The Erw. amylovora pyrC gene encodes a predicted dihydroorotase enzyme involved in pyrimidine biosynthesis. Here, we discovered that the Erw. amylovora pyrC244::Tn5 mutant was a uracil auxotroph. Unexpectedly, the ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12417

    authors: Ramos LS,Sinn JP,Lehman BL,Pfeufer EE,Peter KA,McNellis TW

    更新日期:2015-06-01 00:00:00

  • Novel ecological niche of Cetobacterium somerae, an anaerobic bacterium in the intestinal tracts of freshwater fish.

    abstract:AIMS:This study was conducted to clarify the taxonomic status of Bacteroides type A strains with high vitamin B(12)-producing ability that is widely distributed in the intestinal tracts of freshwater fish. METHODS AND RESULTS:Seventeen strains of Bacteroides type A isolated from five fish species were all rod-shaped a...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02258.x

    authors: Tsuchiya C,Sakata T,Sugita H

    更新日期:2008-01-01 00:00:00

  • Isolation and genome analysis of a lytic Pasteurella multocida Bacteriophage PMP-GAD-IND.

    abstract::Currently used alum precipitated and oil adjuvant vaccines against HS caused by Pasteurella multocida B:2, have side effects and short-lived immunity, leading to regular catastrophic outbreaks in bovines in Asian subcontinent. The need for the development of an improved vaccine with longer immunity and the ability to ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13010

    authors: Qureshi S,Saxena HM,Imam N,Kashoo Z,Sharief Banday M,Alam A,Malik MZ,Ishrat R,Bhat B

    更新日期:2018-09-01 00:00:00

  • Molecular characterization of isolates of Beauveria bassiana obtained from overwintering and summer populations of Sunn Pest (Eurygaster integriceps).

    abstract:AIM:To examine whether isolates of the entomopathogenic fungus Beauveria bassiana are more closely associated to their summer hosts compared with overwintering hosts, with recently developed molecular tools based on mitochondrial regions. METHODS AND RESULTS:Primers for the traditional ITS1-5.8S-ITS2 region and two mi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02331.x

    authors: Kouvelis VN,Ghikas DV,Edgington S,Typas MA,Moore D

    更新日期:2008-03-01 00:00:00

  • Wheat or rye supplemented diets do not affect faecal mucus concentration or the adhesion of probiotic micro-organisms to faecal mucus.

    abstract::In a crossover study, volunteers consumed a diet supplemented with either wheat or rye bread. Levels of faecal mucus were determined and the adhesion of five selected probiotics to immobilized intestinal mucus, was assessed. No significant difference in the concentration of faecal mucus or adhesion of the probiotics t...

    journal_title:Letters in applied microbiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1472-765x.2000.00758.x

    authors: Ouwehand AC,Gråsten S,Niemi P,Mykkänen H,Salminen S

    更新日期:2000-07-01 00:00:00

  • Purification, characterization and application of a novel antimicrobial peptide from Andrias davidianus blood.

    abstract::The Andrias davidianus has been known as a traditional Chinese medicine for a long time. Its blood is considered as a waste or by-product of the meat production industry. Although there are reports on isolation of the antimicrobial peptides from different resources, there are no reports of their isolation from A. davi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12823

    authors: Pei J,Feng Z,Ren T,Sun H,Han H,Jin W,Dang J,Tao Y

    更新日期:2018-01-01 00:00:00

  • Pyruvate relieves the necessity of high induction levels of catalase and enables Campylobacter jejuni to grow under fully aerobic conditions.

    abstract:AIMS:Several cases of campylobacteriosis reported worldwide seemingly conflict with the strict growth requirements and sensitivity to environmental stress of Campylobacter jejuni. In this study, the need for a micro-aerobic environment [dissolved oxygen tension (DOT): 0.1-90%; 100% air saturation)] and the adaptive res...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2008.02326.x

    authors: Verhoeff-Bakkenes L,Arends AP,Snoep JL,Zwietering MH,de Jonge R

    更新日期:2008-03-01 00:00:00

  • Phosphate promotes uranium (VI) adsorption in Staphylococcus aureus LZ-01.

    abstract:UNLABELLED:Staphylococcus aureus LZ-01 was isolated from the Yellow River upstream from Lanzhou which can resist and reduce chromium (VI) to chromium (III). In this study, strain LZ-01's uranium (VI) resistance and adsorption abilities were investigated. Our results showed that it can resist 2 mmol l(-1) U(VI) and adso...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12310

    authors: Zou L,Chen Z,Zhang X,Liu P,Li X

    更新日期:2014-11-01 00:00:00

  • An investigation into the ecological niches and seasonal nature of Clostridium estertheticum and Clostridium gasigenes in the Irish beef farm environment.

    abstract::Blown pack spoilage (BPS) of vacuum packaged beef is caused by psychrotolerant and psychrophilic Clostridium species, primarily Clostridium estertheticum and Clostridium gasigenes. The aim of this study was to investigate the environmental niches and impact of season on these BPS Clostridium spp. on Irish beef farms. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13344

    authors: Esteves E,Whyte P,Gupta TB,Bolton DJ

    更新日期:2020-12-01 00:00:00

  • Degradation of quillaja saponins by mixed culture of rumen microbes.

    abstract::Quillaja saponin (QS) was incubated at 39 degrees C in an in vitro medium containing rumen liquor from a cow fed a roughage diet. No degradation of QS was observed up to 6 h of fermentation. Incubation for 9, 12 and 24 h decreased the content of QS by 16%, 45% and 100%. The content of QS did not decrease when incubate...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00207.x

    authors: Makkar HP,Becker K

    更新日期:1997-10-01 00:00:00

  • Characterization of beta-lactamase and biofilm producing Enterobacteriaceae isolated from organized and backyard farm ducks.

    abstract::This study was undertaken to detect the occurrence of beta-lactamase and biofilm producing Enterobacteriaceae in healthy ducks. A total 202 cloacal swabs were collected from ducks kept in organized (n = 92) and backyard (n = 110) farms in West Bengal (India). The ducks had no history of antibiotic intake. Among the 87...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13170

    authors: Banerjee A,Bardhan R,Chowdhury M,Joardar SN,Isore DP,Batabyal K,Dey S,Sar TK,Bandyopadhyay S,Dutta TK,Samanta I

    更新日期:2019-08-01 00:00:00

  • Modelling the growth and ethanol production of Brettanomyces bruxellensis at different glucose concentrations.

    abstract:AIM:To study the effect of glucose concentrations on the growth by Brettanomyces bruxellensis yeast strain in batch experiments and develop a mathematical model for kinetic behaviour analysis of yeast growing in batch culture. METHODS AND RESULTS:A Matlab algorithm was developed for the estimation of model parameters....

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03081.x

    authors: Aguilar-Uscanga MG,Garcia-Alvarado Y,Gomez-Rodriguez J,Phister T,Delia ML,Strehaiano P

    更新日期:2011-08-01 00:00:00

  • Development and evaluation of a real-time PCR assay targeting chromosomal DNA of Erwinia amylovora.

    abstract:AIMS:To develop and evaluate a new and reliable real-time PCR detection protocol on chromosomal DNA of the contagious plant pathogenic bacterium Erwinia amylovora, the causal agent of fire blight. METHODS AND RESULTS:A Taqman minor-groove-binder real-time PCR assay targeting a hypothetical protein coding gene of Erw. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02892.x

    authors: Gottsberger RA

    更新日期:2010-09-01 00:00:00

  • Detection of live Salmonella enterica in fresh-cut vegetables by a TaqMan-based one-step reverse transcription real-time PCR.

    abstract::Fresh-cut produce is at greater risk of Salmonella contamination. Detection and early warning systems play an important role in reducing the dissemination of contaminated products. One-step Reverse Transcription Polymerase Chain Reaction (RT-qPCR) targeting Salmonella tmRNA with or without a 6-h enrichment was evaluat...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12871

    authors: Miao YJ,Xiong GT,Bai MY,Ge Y,Wu ZF

    更新日期:2018-05-01 00:00:00

  • Discrimination of Arcobacter butzleri isolates by polymerase chain reaction-mediated DNA fingerprinting.

    abstract:AIMS:The objective of this study was to subtype Arcobacter butzleri isolates using RAPD-PCR. METHODS AND RESULTS:Thirty-five A. butzleri isolates obtained from chicken carcasses were examined. PCR-mediated DNA fingerprinting technique with primers of the variable sequence motifs was used to detect polymorphism within ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2002.01152.x

    authors: Atabay HI,Bang DD,Aydin F,Erdogan HM,Madsen M

    更新日期:2002-01-01 00:00:00

  • Identification and characterization of a new three-component nicotinic acid hydroxylase NahAB1 B2 from Pusillimonas sp. strain T2.

    abstract::Nicotinic acid (NA) is ubiquitous in nature and its microbial degradation mechanisms are diverse. In this study, Pusillimonas sp. strain T2 was found to be capable of utilizing NA as sole carbon and nitrogen sources. This strain could completely degrade 300 mg l-1 NA within 3·5 h at 30°C and pH 7·0 and one of the degr...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12850

    authors: Yuan M,Zhang Y,Zhao L,Ma Y,He Q,He J,Qiu J

    更新日期:2018-04-01 00:00:00