Impact of antiseptics on Chlamydia trachomatis growth.

Abstract:

UNLABELLED:Bacterial vaginosis is a frequent dysbiosis, where the normal lactobacillus-dominated flora is replaced by an anaerob/aerob polymicrobial flora. Bacterial vaginosis increases the risk of acquiring sexually transmitted infections (STI) including the most frequent Chlamydia trachomatis infections. Intravaginal antiseptics are part of the bacterial vaginosis treatment, and ideally they should also inhibit the bacterial vaginosis-related STI. Therefore, we tested the antichlamydial activity of four antiseptics: iodine aqueous solution, povidone-iodine, chlorhexidine and borax. First, we measured the impact of antiseptics on the viability of the HeLa cervical epithelial cells, and calculated the maximum nontoxic concentrations. Next, we infected the cells with C. trachomatis preincubated for 1 h with the particular antiseptic. The chlamydial growth was measured by direct quantitative PCR (qPCR) of the infected cells. The minimal inhibitory concentrations (MIC) of chlorhexidine and povidone-iodine were 3·91 and 97 μg ml(-1) respectively; however, the MIC of chlorhexidine was close to its maximum nontoxic concentration. The iodine aqueous solution and the borax showed no antichlamydial activity. Our in vitro studies showed that chlorhexidine and particularly povidone-iodine are potentially able to limit the bacterial vaginosis-related C. trachomatis infection. SIGNIFICANCE AND IMPACT OF THE STUDY:We measured the antichlamydial effects of various antiseptics. These antiseptics are being used for the treatment of bacterial vaginosis, but their effect on the bacterial vaginosis-related sexually transmitted infections, particularly the most frequent Chlamydia trachomatis (C. trachomatis) infections has not been investigated. We showed that povidone-iodine (Betadine) inhibited the chlamydial growth in concentrations that was not toxic to the epithelial cells. We concluded that due to its additional antichlamydial effect, povidone-iodine could be a preferable antiseptic in bacterial vaginosis treatment.

journal_name

Lett Appl Microbiol

authors

Párducz L,Eszik I,Wagner G,Burián K,Endrész V,Virok DP

doi

10.1111/lam.12625

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

260-7

issue

4

eissn

0266-8254

issn

1472-765X

journal_volume

63

pub_type

杂志文章
  • Studies of the release of small molecules during pressure germination of spores of Bacillus subtilis.

    abstract:AIMS:To measure rates of release of small molecules during pressure germination of Bacillus subtilis spores, and the role of SpoVA proteins in dipicolinic acid (DPA) release. METHODS AND RESULTS:Rates of DPA release during B. subtilis spore germination with pressures of 150 or 500 megaPascals were much higher in spore...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02204.x

    authors: Vepachedu VR,Hirneisen K,Hoover DG,Setlow P

    更新日期:2007-09-01 00:00:00

  • Differential activity profiles of translation inhibitors in whole-cell and cell-free approaches.

    abstract:AIMS:Evaluation of the activity profiles of standard prokaryotic translation inhibitors with different physicochemical properties under whole-cell and cell-free conditions. METHODS AND RESULTS:The minimal inhibitory concentration values (cell-free/whole-cell microg ml(-1)) for three aminoglycosides (neomycin, 0.01/6.9...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2007.02281.x

    authors: Weidlich M,Klammt C,Bernhard F,Karas M,Stein T

    更新日期:2008-02-01 00:00:00

  • Presence of sourdough lactic acid bacteria in commercial total mixed ration silage as revealed by denaturing gradient gel electrophoresis analysis.

    abstract:AIMS:To characterize the bacterial communities in commercial total mixed ration (TMR) silage, which is known to have a long bunk life after silo opening. METHODS AND RESULTS:Samples were collected from four factories that produce TMR silage according to their own recipes. Three factories were sampled three times at 1-...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02915.x

    authors: Wang C,Nishino N

    更新日期:2010-10-01 00:00:00

  • Antibacterial and antiviral evaluation of sulfonoquinovosyldiacylglyceride: a glycolipid isolated from Azadirachta indica leaves.

    abstract:UNLABELLED:Assessment of antibacterial as well as antiherpes virus activity of sulfonoquinovosyldiacylglyceride (SQDG), a glycolipid, isolated from the leaves of Azadirachta indica has been described. Antimicrobial activity was evaluated against Gram-positive, Gram-negative bacteria and herpes simplex virus. SQDG showe...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12174

    authors: Bharitkar YP,Bathini S,Ojha D,Ghosh S,Mukherjee H,Kuotsu K,Chattopadhyay D,Mondal NB

    更新日期:2014-02-01 00:00:00

  • Isolation of a strain of Bacillus thuringiensis ssp. kurstaki HD-1 encoding delta-endotoxin Cry1E.

    abstract::A strain of Bacillus thuringiensis, STB-1, toxic against Spodoptera exigua, was isolated. Bacillus thuringiensis STB-1 produced bipyramidal inclusions and reacted with the H antiserum of B. thuringiensis ssp. kurstaki. The plasmid and protein profiles of B. thuringiensis STB-1 were compared with those of its reference...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00355.x

    authors: Chang JH,Roh JY,Je YH,Park HW,Jin BR,Woo SD,Kang SK

    更新日期:1998-05-01 00:00:00

  • Lactobacillus farciminis MD, a newer strain with potential for bacteriocin and antibiotic assay.

    abstract::A native isolate Lactobacillus farciminis MD isolated from fermenting mushroom exhibited a high degree of sensitivity to the majority of the bacteriocins produced by strains of lactobacilli, leuconostoc and pediococci. Also, the efficacy of Lact. farciminis MD as a sensitive strain for antibiotic assay was established...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2000.00691.x

    authors: Halami PM,Chandrashekar A,Nand K

    更新日期:2000-03-01 00:00:00

  • Selection of Salmonella typhimurium as an indicator for pathogen regrowth potential in composted biosolids.

    abstract::In order to select a suitable indicator for monitoring the pathogen regrowth potential of composted biosolids, the growth kinetics of selected bacteria were investigated. Growth parameters of six serovars of Salmonella and three strains of Escherichia coli in sterilized compost were compared. Seeded Salmonella and E. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00626.x

    authors: Sidhu J,Gibbs RA,Ho GE,Unkovich I

    更新日期:1999-11-01 00:00:00

  • An investigation into the ecological niches and seasonal nature of Clostridium estertheticum and Clostridium gasigenes in the Irish beef farm environment.

    abstract::Blown pack spoilage (BPS) of vacuum packaged beef is caused by psychrotolerant and psychrophilic Clostridium species, primarily Clostridium estertheticum and Clostridium gasigenes. The aim of this study was to investigate the environmental niches and impact of season on these BPS Clostridium spp. on Irish beef farms. ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13344

    authors: Esteves E,Whyte P,Gupta TB,Bolton DJ

    更新日期:2020-12-01 00:00:00

  • Antibiotic resistance in Listeria species isolated from catfish fillets and processing environment.

    abstract:AIMS:To investigate the susceptibility of 221 Listeria spp. (86 Listeria monocytogenes, 41 Listeria innocua and 94 Listeria seeligeri-Listeria welshimeri-Listeria ivanovii) isolated from catfish fillets and processing environment to 15 antibiotics. METHODS AND RESULTS:Listeria isolates were analysed by disc-diffusion ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02843.x

    authors: Chen BY,Pyla R,Kim TJ,Silva JL,Jung YS

    更新日期:2010-06-01 00:00:00

  • Degradation of quillaja saponins by mixed culture of rumen microbes.

    abstract::Quillaja saponin (QS) was incubated at 39 degrees C in an in vitro medium containing rumen liquor from a cow fed a roughage diet. No degradation of QS was observed up to 6 h of fermentation. Incubation for 9, 12 and 24 h decreased the content of QS by 16%, 45% and 100%. The content of QS did not decrease when incubate...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00207.x

    authors: Makkar HP,Becker K

    更新日期:1997-10-01 00:00:00

  • Two-dimensional environmental profiles of growth, deoxynivalenol and nivalenol production by Fusarium culmorum on a wheat-based substrate.

    abstract:AIMS:To determine the effect of interacting conditions of water activity (aw, 0.99-0.85), temperature (15, 25 degrees C) and time (40 days) on growth and production of the mycotoxins deoxynivalenol (DON) and nivalenol (NIV) by Fusarium culmorum on a wheat-based agar medium. METHODS AND RESULTS:Fusarium culmorum grew o...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01358.x

    authors: Hope R,Magan N

    更新日期:2003-01-01 00:00:00

  • Antibacterial activity of bovine lactoferrin and its peptides against enterohaemorrhagic Escherichia coli O157:H7.

    abstract::The antimicrobial activities of bovine lactoferrin (bLF), its pepsin hydrolysate (bLFH) and the active peptide lactoferricin B (LFcinB) against four clinical isolates of enterohaemorrhagic Escherichia coli O157:H7 were studied. The MICs against these isolates were 3 mg ml-1 for bLF, 0.1-0.2 mg ml-1 for bLFH and 8-10 m...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00358.x

    authors: Shin K,Yamauchi K,Teraguchi S,Hayasawa H,Tomita M,Otsuka Y,Yamazaki S

    更新日期:1998-06-01 00:00:00

  • Effect of disinfection upon dissolved organic carbon (DOC) in wastewater: bacterial bioassays.

    abstract::Quantitative and qualitative changes in organic matter content of wastewater effluents attributable to chlorination and ozonation have been analysed using bioassays as well as organic carbon direct measures. Bioassays were carried out using the bacterial populations of wastewater and two Escherichia coli strains as te...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.2000.00793.x

    authors: Arana I,Santorum P,Muela A,Barcina I

    更新日期:2000-08-01 00:00:00

  • Mycobactericidal activity of hydrogen peroxide activated by a novel heterogeneous Fentons-like catalyst system.

    abstract::To investigate the potential activation of hydrogen peroxide by a novel catalyst, reducing the concentration of hydrogen peroxide required and the time taken for mycobactericidal activity. The mycobactericidal properties of an iron-based novel heterogeneous-modified polyacrylonitrile (PAN) catalyst in combination with...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12010

    authors: Price SL,Huddersman KD,Shen J,Walsh SE

    更新日期:2013-02-01 00:00:00

  • Glucose metabolism and internal pH of Lactococcus lactis subsp. lactis cells utilizing NMR spectroscopy.

    abstract::The metabolism of glucose was studied in Lactococcus lactis subsp. CNRZ 125 by 13C NMR. The initial rate of glucose utilization was higher for exponential phase cells than for stationary phase cells [150 vs 85 nmol g (dry wt)-1 s -1]. 31P NMR was used to determine changes in glycolytic phosphorylated intermediates (fr...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1995.tb00994.x

    authors: Foucaud C,Herve M,Neumann JM,Hemme D

    更新日期:1995-07-01 00:00:00

  • Utilization of chicory roots for microbial endoinulinase production.

    abstract:AIMS:The optimal culture conditions for endoinulinase production using chicory roots were studied in shake-flask culture. METHODS AND RESULTS:Much higher enzyme production was achieved with Xanthomonas sp. (15 U ml(-1)) than with Pseudomonas sp. (3 U ml(-1)). Optimized culture conditions of Xanthomonas sp. for endoinu...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2001.00977.x

    authors: Park JP,Yun JW

    更新日期:2001-09-01 00:00:00

  • Candida keroseneae sp. nov., a novel contaminant of aviation kerosene.

    abstract:AIMS:To characterize and identify a novel contaminant of aviation fuel. METHODS AND RESULTS:Micro-organisms (yeasts and bacteria) were isolated from samples of aviation fuel. A yeast that proved to have been unrecorded previously was isolated from more than one fuel sample. This novel yeast proved to be a new species ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2010.02968.x

    authors: Buddie AG,Bridge PD,Kelley J,Ryan MJ

    更新日期:2011-01-01 00:00:00

  • Isolation, partial purification and characterization of a bacteriocin produced by a newly isolated Bacillus subtilis strain.

    abstract::A wild type micro-organism producing antibacterial substances has been isolated from a Chinese fermented soybean seasoning and identified as Bacillus subtilis. A crude antibacterial preparation (CABP) was obtained by ammonium sulphate precipitation. Isoelectric focusing assay revealed at least four antimicrobial compo...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2672.1999.00545.x

    authors: Zheng G,Slavik MF

    更新日期:1999-05-01 00:00:00

  • A lack of predatory interaction between rumen ciliate protozoa and Shiga-toxin producing Escherichia coli.

    abstract:AIMS:To investigate interactions between rumen protozoa and Shiga toxin-producing Escherichia coli (STEC) and to ascertain whether it is likely that rumen protozoa act as ruminant hosts for STEC. METHODS AND RESULTS:The presence of stx genes in different microbial fractions recovered from cattle and sheep rumen conten...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2004.01642.x

    authors: Burow LC,Gobius KS,Vanselow BA,Klieve AV

    更新日期:2005-01-01 00:00:00

  • Diagnosis of mycobacterial infections by PCR and restriction enzyme digestion.

    abstract::A method for the diagnosis of mycobacterial infections by PCR amplification followed by selective restriction enzyme digestion of the PCR product was developed. The amplified DNA sequence used in this study occurs within the gene encoding for the mycobacterial 65 kDa heat shock protein (Hance et al. 1989), which is fo...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765x.1992.tb00720.x

    authors: Rodrigo G,Källenius G,Hoffmann E,Svenson SB

    更新日期:1992-08-01 00:00:00

  • The oxidative stress response in Enterococcus faecalis: relationship between H2O2 tolerance and H2O2 stress proteins.

    abstract::The hydrogen peroxide (H2O2) stress response in Enterococcus faecalis ATCC19433 was investigated. A 2.4 mmol l-1 H2O2 pretreatment conferred protection against a lethal concentration (45 mmol l-1) of this agent. The relatively high concentrations of H2O2 used for adaptation and challenge treatments in Ent. faecalis em...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1998.00325.x

    authors: Flahaut S,Laplace JM,Frère J,Auffray Y

    更新日期:1998-04-01 00:00:00

  • Isolation of endophytic diazotroph Pantoea agglomerans and nondiazotroph Enterobacter asburiae from sweetpotato stem in Japan.

    abstract:AIMS:To isolate and identify diazotrophic endophytes in the stem of Japanese sweetpotato cv. Koganesengan. METHODS AND RESULTS:Surface-sterilized and thinly sliced (1-2 mm) sweetpotato stem samples were incubated in test tubes with semi-solid modified Rennie (MR) medium. The test tubes were assayed for acetylene reduc...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.2003.01434.x

    authors: Asis CA Jr,Adachi K

    更新日期:2004-01-01 00:00:00

  • LC-MS/MS quantification of short-chain acyl-CoA's in Escherichia coli demonstrates versatile propionyl-CoA synthetase substrate specificity.

    abstract:AIMS:  This paper utilized quantitative LC-MS/MS to profile the short-chain acyl-CoA levels of several strains of Escherichia coli engineered for heterologous polyketide production. To further compare and potentially expand the levels of available acyl-CoA molecules, a propionyl-CoA synthetase gene from Ralstonia solan...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03184.x

    authors: Armando JW,Boghigian BA,Pfeifer BA

    更新日期:2012-02-01 00:00:00

  • Species identification, virulence markers and antimicrobial resistance profiles of Aeromonas sp. isolated from marketed hard-shelled mussel (Mytilus coruscus) in Korea.

    abstract::Hard-shelled mussel (Mytilus coruscus) is a popular seafood in Korea. This study aimed to determine the virulence markers and antimicrobial resistance patterns of 33 Aeromonas strains isolated from mussels. The isolates were identified as A. salmonicida (n = 14), A. veronii (n = 9), A. enteropelogenes (n = 4), A. cavi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13266

    authors: Hossain S,Wickramanayake MVKS,Dahanayake PS,Heo GJ

    更新日期:2020-03-01 00:00:00

  • Development of a colorimetric colony-screening assay for detection of defluorination by micro-organisms.

    abstract:AIMS:To develop a colorimetric colony-screening assay to facilitate the isolation of micro-organisms capable of defluorination. METHODS AND RESULTS:A metal-dye chelate, zirconium-xylenol orange was used to detect fluoride ions released from a fluorinated substrate through microbial metabolism. Depolymerised zirconium ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2011.03123.x

    authors: Davis CK,Denman SE,Sly LI,McSweeney CS

    更新日期:2011-10-01 00:00:00

  • Alteration of crop rotation in continuous Pinellia ternate cropping soils profiled via fungal ITS amplicon sequencing.

    abstract::Pinellia ternata is a traditional Chinese herb that has been shown to suffer from continuous cropping. Deterioration in continuous P. ternata cropping soils and the effect of alteration of crop rotation on the soil were evaluated by using Illumina high-throughput sequencing technology. Soil physicochemical properties ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.13135

    authors: He Z,Chen H,Liang L,Dong J,Liang Z,Zhao L

    更新日期:2019-06-01 00:00:00

  • Phenotypic and genotypic characterization of H2 S-positive and H2 S-negative strains of Shewanella baltica isolated from spoiled whiting (Merlangius merlangus).

    abstract:UNLABELLED:Four strains were isolated from a spoiled whiting (Merlangius merlangus). All of them were able to grow aerobically from 4 to 30°C and also able to develop anaerobically in the presence of trimethylamine N-Oxide (TMAO) at 25°C. Biochemical characterization did not allow identification of the strains species ...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/lam.12312

    authors: Dehaut A,Midelet-Bourdin G,Brisabois A,Duflos G

    更新日期:2014-11-01 00:00:00

  • Wheat or rye supplemented diets do not affect faecal mucus concentration or the adhesion of probiotic micro-organisms to faecal mucus.

    abstract::In a crossover study, volunteers consumed a diet supplemented with either wheat or rye bread. Levels of faecal mucus were determined and the adhesion of five selected probiotics to immobilized intestinal mucus, was assessed. No significant difference in the concentration of faecal mucus or adhesion of the probiotics t...

    journal_title:Letters in applied microbiology

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1046/j.1472-765x.2000.00758.x

    authors: Ouwehand AC,Gråsten S,Niemi P,Mykkänen H,Salminen S

    更新日期:2000-07-01 00:00:00

  • Gene expression and characteristics of a novel fibrinolytic enzyme (subtilisin DFE) in Escherichia coli.

    abstract:AIMS:The objective of this study is to actively express a novel fibrinolytic enzyme, subtilisin DFE (douchi fibrinolytic enzyme), in Escherichia coli. METHODS AND RESULTS:The DNA fragments encoding pro-subtilisin DFE was amplified and cloned into the vector pET32a to obtain N-terminal Trx fusion expression plasmid. Th...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1472-765X.2005.01715.x

    authors: Zhang RH,Xiao L,Peng Y,Wang HY,Bai F,Zhang YZ

    更新日期:2005-01-01 00:00:00

  • Survival of Escherichia coli O157:H7 in yoghurt during preparation and storage at 4 degrees C.

    abstract::Cow's milk was inoculated with ca 10(3) and 10(7) cfu ml-1 Escherichia coli O157:H7. After fermentation at 42 degrees C for 0-5 h, the yoghurt was stored at 4 degrees C. Two kinds of yoghurt were used: traditional yoghurt (TY), made with Streptococcus thermophilus and Lactobacillus bulgaricus starter cultures, and 'bi...

    journal_title:Letters in applied microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1472-765x.1997.00067.x

    authors: Massa S,Altieri C,Quaranta V,De Pace R

    更新日期:1997-05-01 00:00:00