Pulsed electric field inactivation of diarrhoeagenic Bacillus cereus through irreversible electroporation.

Abstract:

:The physical effects of high-intensity pulsed electric fields (PEF) on the inactivation of diarrhoeagenic Bacillus cereus cells suspended in 0.1% peptone water were examined by transmission electron microscopy (TEM). The levels of PEF-induced microbial cell death were determined by enumeration on tryptone soy yeast extract agar and Bacillus cereus-selective agar plates. Following exposure to lethal levels of PEF, TEM investigation revealed irreversible cell membrane rupture at a number of locations, with the apparent leakage of intracellular contents. This study provides a clearer understanding of the mechanism of PEF-induced cellular damage, information that is essential for the further optimization of this emerging food-processing technology.

journal_name

Lett Appl Microbiol

authors

Rowan NJ,MacGregor SJ,Anderson JG,Fouracre RA,Farish O

doi

10.1046/j.1365-2672.2000.00772.x

subject

Has Abstract

pub_date

2000-08-01 00:00:00

pages

110-4

issue

2

eissn

0266-8254

issn

1472-765X

pii

lam772

journal_volume

31

pub_type

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