Evaluation of the specificity of Salmonella PCR primers using various intestinal bacterial species.

abstract：:A study was made of the incidence of Campylobacter upsaliensis among dogs and cats suffering from acute or chronic diarrhoea; 225 dogs and cats were examined and 51 strains were identified, 16 (7%) of which were Camp. upsaliensis. When rectal swabs were taken from a control group of 126 dogs and cats without clinical ...

journal_title：Letters in applied microbiology

authors： Steinhauserova I,Fojtikova K,Klimes J

更新日期：2000-09-01 00:00:00

abstract：AIMS:To investigate the susceptibility of 221 Listeria spp. (86 Listeria monocytogenes, 41 Listeria innocua and 94 Listeria seeligeri-Listeria welshimeri-Listeria ivanovii) isolated from catfish fillets and processing environment to 15 antibiotics. METHODS AND RESULTS:Listeria isolates were analysed by disc-diffusion ...

journal_title：Letters in applied microbiology

authors： Chen BY,Pyla R,Kim TJ,Silva JL,Jung YS

更新日期：2010-06-01 00:00:00

abstract：UNLABELLED:Bioconversion of biomass, particularly crop wastes, into biofuels is being developed as an alternative approach in meeting the high energy demand. In this study, a thermophilic bacterial strain BY-3 that exhibits cellulolytic potential was isolated from faecal samples of Tibetan pigs; this strain was identif...

journal_title：Letters in applied microbiology

authors： Meng F,Ma L,Ji S,Yang W,Cao B

更新日期：2014-09-01 00:00:00

abstract：:While the beta-glucuronidase activity of intact cells of Clostridium perfringens was higher in 0.95% sodium chloride (NaCl) than that in 0, 0.1 or 0.5%, that of Escherichia coli was higher in 0.1% NaCl than that in 0, 0.5 or 0.95% NaCl in 0.1 mol l-1 KH2PO4. However, the enzyme activity of both species of intact cells...

journal_title：Letters in applied microbiology

authors： Fujisawa T,Aikawa K,Takahashi T,Yamai S

更新日期：2000-09-01 00:00:00

abstract：:Alzheimer's disease (AD) is the most common form of dementia and one of the major causes of disability and dependency in older people. Accumulating evidences link gut microbiota with different diseases and its relationship with neurodegenerative diseases is becoming most intriguing. This study was aimed to compare the...

journal_title：Letters in applied microbiology

authors： Bäuerl C,Collado MC,Diaz Cuevas A,Viña J,Pérez Martínez G

更新日期：2018-06-01 00:00:00

abstract：AIM:The aim of this work was to evaluate the effect of Planococcus ficus infection in red wine grapes on Aspergillus section Nigri and ochratoxin A (OTA) contamination. METHODS AND RESULTS:During 2006/2007 and 2008/2009 vintages, Merlot, Malbec and Cabernet Sauvignon varieties divided into two categories of grape samp...

journal_title：Letters in applied microbiology

authors： Chiotta ML,Ponsone ML,Torres AM,Combina M,Chulze SN

更新日期：2010-08-01 00:00:00

abstract：:Coffee (Coffea arabica L.) is currently grown in many tropical and subtropical areas countries and is a major traded commodity for the developing world. Coffee leaf blight, caused by Phomopsis heveicola, is one of the most important fungal diseases dangerous to coffee crops in China. This study aimed to develop a PCR-...

journal_title：Letters in applied microbiology

authors： Lu Y,Wu WH,He CP,Liang YQ,Huang X,Zheng JL,Xi JG,Tang SB,Yi KX

更新日期：2020-09-26 00:00:00

abstract：AIMS:This study sought to evaluate the performance of two chromogenic media designed for the isolation of vancomycin-resistant enterococci (VRE) and compare them with a traditional bile-esculin medium for the isolation of VRE from stool samples. METHODS AND RESULTS:A total of 285 stool samples were inoculated onto Chr...

journal_title：Letters in applied microbiology

authors： Asir K,Wilkinson K,Perry JD,Reed RH,Gould FK

更新日期：2009-02-01 00:00:00

abstract：AIM:The automated TEMPO system (bioMerieux) is based on the most probable number (MPN) method for the enumeration of micro-organisms in foods. In this study, we evaluated the performance of the TEMPO system as a diagnostic tool in comparison with the standard method in processed soy products. METHODS AND RESULTS:A ver...

journal_title：Letters in applied microbiology

authors： Katase M,Tsumura K

更新日期：2011-11-01 00:00:00

abstract：AIMS:This study was designed to evaluate the prevalence of Clostridium difficile contamination of retail chicken. METHODS AND RESULTS:Chicken legs, thighs and wings were purchased using a standardized method from retail outlets across Ontario, Canada. Selective culture was used for qualitative and quantitative detecti...

journal_title：Letters in applied microbiology

authors： Weese JS,Reid-Smith RJ,Avery BP,Rousseau J

更新日期：2010-04-01 00:00:00

abstract：UNLABELLED:Isolation of local strains is mandatory for the success of control programs. However, clinical samples are typically contaminated by other bacteria, which impair leptospires growth. The purpose of this study was to evaluate the use of a previously reported EMJH-STAFF media in the recovery of pathogenic lepto...

journal_title：Letters in applied microbiology

authors： Loureiro AP,Martins G,Pinto P,Narduche L,Teixeira RC,Lilenbaum W

更新日期：2015-12-01 00:00:00

abstract：AIMS:To isolate, characterize and identify lactic acid bacteria (LAB) in the vineyards where koshu grapes, a primary wine grape cultivar in Japan, are grown. METHODS AND RESULTS:Sixty samples, including leaves, undamaged grape berries and soil under damaged berries, were collected at four koshu vineyards in Yamanashi ...

journal_title：Letters in applied microbiology

authors： Yanagida F,Srionnual S,Chen YS

更新日期：2008-08-01 00:00:00

abstract：AIMS:The aim of this work was to optimize the production of a novel antimaralial menisporopsin A by the seed fungus Menisporopsis theobromae BCC 4162. METHODS AND RESULTS:Fungal cultures were grown in shake flasks at 25 degrees C in the basal medium with varying carbon and nitrogen sources, aeration rates and initial ...

journal_title：Letters in applied microbiology

authors： Madla S,Kittakoop P,Wongsa P

更新日期：2006-11-01 00:00:00

abstract：:Three groups of substances were identified which inhibit the bactericidal activity of bacitracin. Beside two divalent metals, Mg2+ and Ca2+, and a metabolite with chelating properties, citrate, the most pronounced effect was observed with pyrophosphate. Metals probably prevent access of bacitracin to the lipid carrier...

journal_title：Letters in applied microbiology

authors： Podlesek Z,Comino A

更新日期：1994-08-01 00:00:00

abstract：AIMS:To investigate the ability of a mixture of phage K and six of its modified derivatives to prevent biofilm formation by Staphylococcus aureus and also to reduce the established biofilm density. METHODS AND RESULTS:The bioluminescence-producing Staph. aureus Xen29 strain was used in the study, and incubation of thi...

journal_title：Letters in applied microbiology

authors： Kelly D,McAuliffe O,Ross RP,Coffey A

更新日期：2012-04-01 00:00:00

abstract：AIMS:Adaptation of a colorimetric assay using [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] (MTT) to evaluate the cytotoxicity of the extracellular products of micro-organisms pathogenic to fish. METHODS AND RESULTS:The optimal conditions for the colorimetric assay were determined and this method was...

journal_title：Letters in applied microbiology

authors： Zorrilla I,Balebona MC,Moriñigo MA

更新日期：2001-11-01 00:00:00

abstract：:Cow's milk was inoculated with ca 10(3) and 10(7) cfu ml-1 Escherichia coli O157:H7. After fermentation at 42 degrees C for 0-5 h, the yoghurt was stored at 4 degrees C. Two kinds of yoghurt were used: traditional yoghurt (TY), made with Streptococcus thermophilus and Lactobacillus bulgaricus starter cultures, and 'bi...

journal_title：Letters in applied microbiology

authors： Massa S,Altieri C,Quaranta V,De Pace R

更新日期：1997-05-01 00:00:00

abstract：:The cell surface hydrophobicity of micro-organisms is a characteristic that has been associated with the colonization of mammalian epithelia and with their capacity to induce diseases. Melatonin is a hormone produced by the pineal gland that affects the immune response mechanism. This study investigated, as an express...

journal_title：Letters in applied microbiology

authors： Uberos J,Molina A,Liébana J,Augustin MC,Muñoz A

更新日期：2000-10-01 00:00:00

abstract：AIMS:The aim of this study was to determine the antimicrobial and antiadhesive properties of a biosurfactant isolated from Lactobacillus paracasei ssp. paracasei A20 against several micro-organisms, including Gram-positive and Gram-negative bacteria, yeasts and filamentous fungi. METHODS AND RESULTS:Antimicrobial and ...

journal_title：Letters in applied microbiology

authors： Gudiña EJ,Rocha V,Teixeira JA,Rodrigues LR

更新日期：2010-04-01 00:00:00

abstract：AIMS:To simplify the electrotransformation process of Clostridium acetobutylicum, which currently needs to be performed in an anaerobic chamber, thus laborious and time-consuming. METHODS AND RESULTS:The CAC2634 gene encoding PerR is a known peroxide regulon repressor in Cl. acetobutylicum. CAC2634 in a previously con...

journal_title：Letters in applied microbiology

authors： Dong H,Tao W,Zhu L,Zhang Y,Li Y

更新日期：2011-09-01 00:00:00

abstract：AIMS:Species-specific PCR was applied to identify Lactobacillus brevis and the sensitivity and the specificity of the protocol were determined. METHODS AND RESULTS:Strains of Lact. brevis obtained from foods, particularly dairy products, and various strain collections, were identified by PCR using primers which amplif...

journal_title：Letters in applied microbiology

authors： Guarneri T,Rossetti L,Giraffa G

更新日期：2001-11-01 00:00:00

abstract：:Leuconostoc strains were characterized according to their antibiotic susceptibilities, carbohydrate fermentation profiles, sucrase activity patterns and plasmid content. All the strains tested were resistant to the antibiotics sulphathiazole, trimethoprim and vancomycin, and could be separated into two groups based on...

journal_title：Letters in applied microbiology

authors： Holt SM,Al-Sheikh H,Shin KJ

更新日期：2001-03-01 00:00:00

abstract：:Pyrimidine synthesis in the food spoilage agent Burkholderia cepacia ATCC 25416 was investigated. The five de novo pathway enzymes of pyrimidine biosynthesis were found to be active in B. cepacia ATCC 25416 and growth of this strain on uracil had an effect on the de novo enzyme activities. The in vitro regulation of a...

journal_title：Letters in applied microbiology

authors： Li K,West TP

更新日期：1995-11-01 00:00:00

abstract：:Emulsions of the fatty acids linoleic (C18:2 n-6), alpha-linolenic (C18:3 n-3) and arachidonic acid (C20:4 n-6) were incubated for 4 h under anaerobic conditions with human faecal suspensions. Linoleic acid was significantly decreased (P < 0.001) and there was a significant rise (P < 0.05) in its hydrogenation product...

journal_title：Letters in applied microbiology

authors： Howard FA,Henderson C

更新日期：1999-09-01 00:00:00

abstract：AIMS:Bacillus halodurans C-125 is a Gram-positive bacterium that was the first alkaliphilic species to have its genome completely sequenced. Despite its many years as a model for alkaliphily and source of industrially important enzymes, genetic manipulation of B. halodurans C-125 remains difficult, and therefore, we so...

journal_title：Letters in applied microbiology

authors： Wallace JG,Breaker RR

更新日期：2011-04-01 00:00:00

abstract：:Sodium pyrithione and zinc pyrithione (NaPT and ZnPT, respectively) are widely used as cosmetic preservatives and metal chelating agents. They are commonly assayed using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). However, a simple quantitative colorimetric assay has not been pre...

journal_title：Letters in applied microbiology

authors： Dinning AJ,al-Adham IS,Austin P,Collier PJ

更新日期：1998-07-01 00:00:00

abstract：AIMS:To demonstrate that produce rinsates used for RT-qPCR detection of foodborne viruses may cause significant PCR inhibition and propose a means to reduce its impact on sensitivity. METHODS AND RESULTS:Here, it is shown that rinsing and concentration from spinach and precut lettuce have the potential to generate RNA...

journal_title：Letters in applied microbiology

authors： Plante D,Bélanger G,Leblanc D,Ward P,Houde A,Trottier YL

更新日期：2011-03-01 00:00:00

abstract：AIMS:The study evaluated the efficacy of four Mycobacterium avium subsp. paratuberculosis (MAP) culture media in suppressing commonly used starter cultures and typical nonstarter microflora present during the manufacture and ripening of Cheddar cheese, with a view to identify a suitable medium for the enumeration of MA...

journal_title：Letters in applied microbiology

authors： Donaghy JA,Totton NL,Rowe MT

更新日期：2003-01-01 00:00:00

abstract：AIMS:Clostridium perfringens is recommended as a suitable indicator bacterium for human enteric viruses, Giardia cysts and Cryptosporidium oocysts in finished water and in the assessment and evaluation of water treatment. Several agars and confirmation procedures were evaluated in parallel with the Australian/New Zeala...

journal_title：Letters in applied microbiology

authors： Wohlsen T,Bayliss J,Gray B,Bates J,Katouli M

更新日期：2006-05-01 00:00:00

abstract：:Entamoeba histolytica and Entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. The former is a pathogen, whereas the latter is a nonpathogenic commensal. Using a model system of in vitro cocultures and PCR detection of the Entamoeba species, we found that the nonpathogenic spec...

journal_title：Letters in applied microbiology

authors： Pysova I,Tumova P,Tolarova V,Nohynkova E

更新日期：2009-04-01 00:00:00