Abstract:
:Two candidate DNA vaccines based on the proteins CFP10 and CFP21 encoded by regions of difference (RDs) of Mycobacterium tuberculosis were evaluated individually and in multivalent combination with the immunodominant protein Ag85B for induction of protective immune responses against experimental tuberculosis. Experimental DNA vaccines induced substantial levels of cell-mediated immune responses as indicated by marked lymphocyte proliferation, significant release of the Th1 cytokines IFN-gamma and IL-12 (p40), and predominant cytotoxic T cell activity. High levels of antigen-specific IgG1 and IgG2a antibodies observed in the sera of immunized mice depicted strong humoral responses generated by DNA vaccine constructs. The multivalent combination of three DNA vaccine constructs induced maximal T cell and humoral immune responses. All the experimental vaccines imparted significant protection against challenge with M. tuberculosis H(37)Rv (in terms of colony-forming unit reduction in lungs and spleen) as compared to vector controls. The level of protection exhibited by multivalent DNA vaccine formulation was found to be equivalent to that of Mycobacterium bovis BCG observed both at 4 and 8 weeks post-challenge. These results show the protective potential of the multivalent DNA vaccine formulation used in this study.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Grover A,Ahmed MF,Singh B,Verma I,Sharma P,Khuller GKdoi
10.1016/j.micinf.2006.04.025subject
Has Abstractpub_date
2006-08-01 00:00:00pages
2390-9issue
9-10eissn
1286-4579issn
1769-714Xpii
S1286-4579(06)00235-8journal_volume
8pub_type
杂志文章abstract::We previously showed the possibility that Gag A146P, which is an escape mutant from HLA-B∗57-restricted CTLs, was selected by HLA-B∗48:01-restricted Gag138-147(LI10)-specific CTLs in a Japanese cohort in which HLA-B∗57 individuals were not detected. We herein demonstrated Gag140-147(GI8) to be the optimal epitope rath...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2011.03.009
更新日期:2011-08-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(00)01361-7
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pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(01)01389-2
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2013.08.004
更新日期:2013-11-01 00:00:00
abstract::The enteropathogenic Escherichia coli (EPEC) effector protein EspG, like the Shigella effector VirA, functions through disruption of the host cell microtubule network. Reports have differed as to whether the EspG homologue, EspG2, is also responsible for microtubule disruption. In this study we show that following tra...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.04.004
更新日期:2006-07-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2012.02.001
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.11.015
更新日期:2009-02-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.06.010
更新日期:2006-09-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2007.01.010
更新日期:2007-04-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(00)01317-4
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(02)01635-0
更新日期:2002-09-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2016.07.006
更新日期:2016-12-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2013.04.001
更新日期:2013-07-01 00:00:00
abstract::Effective vaccines against infectious diseases and biological warfare agents remain an urgent public health priority. Studies have characterized the differentiation of effector and memory T cells and identified a subset of T cells capable of conferring enhanced protective immunity against pathogen challenge. We hypoth...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2007.07.009
更新日期:2007-10-01 00:00:00
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journal_title:Microbes and infection
pub_type: 信件
doi:10.1016/j.micinf.2020.04.004
更新日期:2020-01-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.07.032
更新日期:2008-10-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2016.08.001
更新日期:2016-12-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2005.06.019
更新日期:2006-01-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2005.12.016
更新日期:2006-04-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2012.11.004
更新日期:2013-02-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.08.003
更新日期:2006-10-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.08.013
更新日期:2006-10-01 00:00:00
abstract::Exhaustive information on the Epstein-Barr virus, a member of the herpes family, is described at the International Herpes Management Forum web-site. Cervical cancer associations, AIDS treatment projects, and the Los Alamos National Laboratories provide useful information on papillomavirus infections, as well as hyperl...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(00)01308-3
更新日期:2000-10-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2017.04.001
更新日期:2017-06-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.03.006
更新日期:2008-05-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2015.10.006
更新日期:2016-02-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
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更新日期:2002-03-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(02)01603-9
更新日期:2002-07-01 00:00:00
abstract::Despite the development of new treatments, the mortality due to invasive pulmonary aspergillosis remains above 50%, reaching 95% in certain situations. The battle against Aspergillus fumigatus involves several components of the pulmonary innate immune system: cells, mediators, and natural antifungal molecules involved...
journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2009.07.002
更新日期:2009-10-01 00:00:00
abstract::The identification of Brachyspira hyodysenteriae outer membrane proteins (OMPs) that may stimulate immunity to swine dysentery is important for vaccine development. We report here the analysis of a novel locus, blpGFEA, encoding four tandem paralogous proteins of approximately 30 kDa from B. hyodysenteriae. The four p...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/s1286-4579(03)00027-3
更新日期:2003-04-01 00:00:00