Abstract:
:Intramuscular immunization with DNA vaccines has been shown to induce a broad range of immune responses and protective immunity in many animal models, but it is less effective in primates. One reason for this may be the low expression of vector-encoded antigen in cells. Here we report that the use of vaccine vector (pCJ-3) containing two regulatory elements, a chimeric intron and a bovine growth hormone (BGH) polyadenylation signal, markedly increased antigen expression both in vitro and in vivo. A positive correlation was seen between the level of expression of Japanese encephalitis virus (JEV) envelope proteins and the levels of antibodies in C3H/HeN mice. Immunization of mice with pCJ-3/ME (pCJ-3 containing the entire membrane and envelope protein genes) with or without cardiotoxin pretreatment resulted in higher antibody titers than immunization with vector containing only envelope protein and conferred full protection against infection with JEV. Electron microscopy showed that pCJ-3/ME expression resulted in the production of virus-like particles of JEV in vitro. The particles enhanced the production of higher titers of neutralizing antibodies and thus provided immunity against JEV. Consequently, the efficacy of the newly developed DNA vaccines was validated. This should pave the way to clinical trials in man.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Wu CJ,Li TL,Huang HW,Tao MH,Chan YLdoi
10.1016/j.micinf.2006.06.010subject
Has Abstractpub_date
2006-09-01 00:00:00pages
2578-86issue
11eissn
1286-4579issn
1769-714Xpii
S1286-4579(06)00265-6journal_volume
8pub_type
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
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journal_title:Microbes and infection
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2010.04.002
更新日期:2010-08-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/j.micinf.2009.03.004
更新日期:2009-05-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2008.01.012
更新日期:2008-04-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2005.06.022
更新日期:2006-01-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章,评审
doi:10.1016/s1286-4579(00)00304-x
更新日期:2000-03-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2003-05-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
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更新日期:2002-07-01 00:00:00
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journal_title:Microbes and infection
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journal_title:Microbes and infection
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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journal_title:Microbes and infection
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doi:10.1016/j.micinf.2009.10.002
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journal_title:Microbes and infection
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更新日期:2012-01-01 00:00:00
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journal_title:Microbes and infection
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doi:10.1016/j.micinf.2011.07.008
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journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2009.03.001
更新日期:2009-04-01 00:00:00
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journal_title:Microbes and infection
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abstract::Phocid herpesvirus virus type 2 (PhHV-2), a putative gammaherpesvirus of seals, has been isolated from harbor seals (Phoca vitulina) and grey seals (Halichoerus grypus). In the present study, different PhHV-2 isolates were shown to have a broad in vitro tropism for various cell types from several mammalian species. In...
journal_title:Microbes and infection
pub_type: 杂志文章
doi:10.1016/j.micinf.2006.10.006
更新日期:2007-01-01 00:00:00
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journal_title:Microbes and infection
pub_type: 杂志文章
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更新日期:2002-11-01 00:00:00