Abstract：
:Pneumococcal serine-rich repeat protein (PsrP) is a glycoprotein that mediates Streptococcus pneumoniae attachment to lung cells and promotes biofilm formation. Herein, we investigated the transcriptional organization of psrP-secY2A2, the 37-kbp pathogenicity island encoding PsrP and its accessory genes. PCR amplification of cDNA and RNA-seq analysis found psrP-secY2A2 to be minimally composed of three operons: psrP-glyA, glyB, and glyC-asp5. Transcription of all three operons was greatest during biofilm growth and immunoblot analyses confirmed increased PsrP production by biofilm pneumococci. Using gas chromatography-mass spectrometry we identified monomeric N-acetylglucosamine as the primary glycoconjugate present on a recombinant intracellular version of PsrP, i.e. PsrP1-734. This finding was validated by immunoblot using lectins with known carbohydrate specificities. We subsequently deleted gtfA and gtfB, the GTFs thought to be responsible for addition of O-linked N-acetylglucosamine, and tested for PsrP and its associated virulence properties. These deletions negatively affected our ability to detect PsrP1-734 in bacterial whole cell lysates. Moreover, S. pneumoniae mutants lacking these genes pheno-copied the psrP mutant and were attenuated for: biofilm formation, adhesion to lung epithelial cells, and pneumonia in mice. Our studies identify the transcriptional organization of psrP-secY2A2 and show the indispensable role of GtfA and GtfB on PsrP-mediated pneumococcal virulence.
journal_name
Microbes Infectjournal_title
Microbes and infectionauthors
Lizcano A,Akula Suresh Babu R,Shenoy AT,Saville AM,Kumar N,D'Mello A,Hinojosa CA,Gilley RP,Segovia J,Mitchell TJ,Tettelin H,Orihuela CJdoi
10.1016/j.micinf.2017.04.001subject
Has Abstractpub_date
2017-06-01 00:00:00pages
323-333issue
6eissn
1286-4579issn
1769-714Xpii
S1286-4579(17)30043-6journal_volume
19pub_type
杂志文章abstract：:Intensive care unit (ICU)-acquired lower respiratory tract infections include acute tracheobronchitis and hospital-acquired and ventilator-associated pneumonia (VAP). Nosocomial pneumonia is the second most common hospital-acquired infection and the leading cause of death in hospital-acquired infections. The mortality...
journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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journal_title：Microbes and infection
pub_type： 杂志文章
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
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journal_title：Microbes and infection
pub_type： 杂志文章,评审
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journal_title：Microbes and infection
pub_type： 杂志文章
doi：10.1016/j.micinf.2005.09.016
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abstract：:Infection with Brucella abortus induces a pro-inflammatory response that drives T cell responses toward a Th1 profile. The mechanism by which this bacterium triggers this response is unknown. Dendritic cells (DC) are crucial mediators at the host-pathogen interface and are potent Th1-inducing antigen-presenting cells....
journal_title：Microbes and infection
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更新日期：2008-10-01 00:00:00
