当前位置： SCI文献检索 > JOURNAL OF NEUROIMMUNOLOGY期刊下所有文献 > Expression of mRNA for multiple serotonin (5-HT) receptor types/subtypes by the peripheral blood mononuclear cells of rhesus macaques.

Expression of mRNA for multiple serotonin (5-HT) receptor types/subtypes by the peripheral blood mononuclear cells of rhesus macaques.

Abstract：

:To find out whether rhesus macaque peripheral blood mononuclear cells (PBMCs) express mRNA for 5-HT receptors, blood samples from normal healthy rhesus monkeys were used to isolate PBMCs by Ficoll-paque density gradient centrifugation. Total RNA was extracted from MT-2 cells, Hut-78 cells, naive or phytohemagglutinin (PHA) stimulated human and monkey PBMCs. One tube RT-PCR was performed using primers specific for human 5-HT1A, 5-HT1B, 5-HT1E, 5-HT2A, 5-HT2B, 5-HT2C, 5-HT3, 5-HT4, 5-HT6, and 5-HT7 receptors. Amplicons of expected sizes were obtained from human cell lines as well as both human and monkey PBMCs. Both PHA stimulated human and monkey PBMCs express mRNAs for 5-HT1A, 5-HT1B, 5-HT1E, 5-HT2A, 5-HT3, 5-HT4, 5-HT6 receptor types/subtypes. However, mRNAs for 5-HT1B, 5-HT1E and 5-HT2A cannot be confidently detected in some of the PBMC samples without PHA stimulation. 5-HT2B and 5-HT7 receptor mRNA was not detected in most of the samples and 5-HT2C receptor mNRA was not detected at all. FACS analysis revealed that CD3+ lymphocyte increased more than 20% among lymphocytes in the PHA stimulated PBMCs. These data indicate that similar to human PBMC, rhesus macaque PBMC may express multiple types of 5-HT receptors and the expression profile could change after PHA stimulation due to either the changes in cell composition or changes in gene transcription level. This provided a basis for further studies on the neuroimmunomodulatory interactions of 5-HT in rhesus macaques.

journal_name

J Neuroimmunol

journal_title

Journal of neuroimmunology

authors

Yang GB,Qiu CL,Zhao H,Liu Q,Shao Y

doi

10.1016/j.jneuroim.2006.05.016

subject

Has Abstract

pub_date

2006-09-01 00:00:00

pages

24-9

issue

1-2

eissn

0165-5728

issn

1872-8421

pii

S0165-5728(06)00202-5

journal_volume

178

pub_type

杂志文章

相关文献

JOURNAL OF NEUROIMMUNOLOGY文献大全
  • MP4- and MOG:35-55-induced EAE in C57BL/6 mice differentially targets brain, spinal cord and cerebellum.

    abstract：:Mechanism-oriented studies of EAE rely mostly on gene-modified mice on the C57BL/6 background. Here we report that MP4-induced EAE displays characteristic differences in CNS pathology as compared to MOG peptide 35-55-elicited disease. While in the latter, the topology of CNS infiltration remained unchanged throughout ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2007.06.016

    authors： Kuerten S,Kostova-Bales DA,Frenzel LP,Tigno JT,Tary-Lehmann M,Angelov DN,Lehmann PV

    更新日期：2007-09-01 00:00:00

  • Attempted isolation of viruses from myasthenia gravis thymus.

    abstract：:A systematic study of thymus homogenates and cell suspensions from 13 patients with myasthenia gravis (MG) of recent onset, and 6 non-myasthenic controls, has failed to detect or isolate virus by cell culture with 'rescue techniques', electron microscopy, or intracerebral inoculation into neonatal mice. These results ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(86)90082-2

    authors： Klavinskis LS,Willcox HN,Richmond JE,Newsom-Davis J

    更新日期：1986-06-01 00:00:00

  • Evidence that mouse astrocytes may be derived from the radial glia. An immunohistochemical study of the cerebellum in the normal and reeler mouse.

    abstract：:Astrocytic cells of unusual aspect can be detected in the cerebellum of normal mice during the first 4 weeks of life. They are visualized with anti-GFAP (glial fibrillary acidic protein), anti-S100 and anti-vimentin immune sera. Their perikaryons, located in the white matter or in the granular layer, extend long proce...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(85)80009-6

    authors： Benjelloun-Touimi S,Jacque CM,Derer P,De Vitry F,Maunoury R,Dupouey P

    更新日期：1985-07-01 00:00:00

  • Wallerian degeneration induces Ia-antigen expression in the rat brain.

    abstract：:Strong expression of class II major histocompatibility (MHC II, Ia) antigens was observed in areas of Wallerian degeneration following either a cryoinjury to the cerebral and cerebellar cortex or unilateral eye enucleation in adult Wistar and Lewis rats. The Wallerian degeneration was disclosed by the Fink-Heimer meth...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(89)90132-x

    authors： Konno H,Yamamoto T,Iwasaki Y,Suzuki H,Saito T,Terunuma H

    更新日期：1989-12-01 00:00:00

  • alpha-Tocopherol reduces lipopolysaccharide-induced peroxide radical formation and interleukin-6 secretion in primary murine microglia and in brain.

    abstract：:The purpose of this study was to determine if alpha-tocopherol-a reactive oxygen species (ROS) scavenging agent-inhibits LPS-induced oxidative stress and interleukin-6 (IL-6) production in murine microglia and brain. LPS increased intracellular peroxides and IL-6 in a dose-dependent fashion in primary microglia. The L...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2003.12.017

    authors： Godbout JP,Berg BM,Kelley KW,Johnson RW

    更新日期：2004-04-01 00:00:00

  • Family-based association study of interleukin 6 (IL6) and its receptor (IL6R) functional polymorphisms in schizophrenia in the Polish population.

    abstract：:Schizophrenia is a heterogeneous disorder and its etiology remains incompletely elucidated. Among possible causes, immunological factors have been implicated in its pathogenesis and course. The inflammatory system may trigger or modulate the course of schizophrenia through complex mechanisms influencing neurodevelopme...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2014.09.019

    authors： Kapelski P,Skibinska M,Maciukiewicz M,Pawlak J,Permoda-Osip A,Twarowska-Hauser J

    更新日期：2015-08-15 00:00:00

  • A novel protective model against experimental allergic encephalomyelitis in mice expressing a transgenic TCR-specific for myelin oligodendrocyte glycoprotein.

    abstract：:Myelin oligodendrocyte glycoprotein (MOG) is an important autoantigen in multiple sclerosis and in experimental autoimmune encephalomyelitis (EAE). We generated a T cell receptor (TCR) transgenic (Tg) mouse expressing a TCR derived from an encephalitogenic T cell clone specific for MOG(35-55). This mouse failed to dev...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2003.12.007

    authors： Mendel I,Natarajan K,Ben-Nun A,Shevach EM

    更新日期：2004-04-01 00:00:00

  • Prevalence of Small Intestinal Bacterial Overgrowth in Multiple Sclerosis: a Case-Control Study from China.

    abstract：OBJECTIVE:It's hypothesized that gastrointestinal microbiota might play an important role in pathogenesis of multiple sclerosis (MS). The aim of the present study was to assess the prevalence of small intestinal bacterial overgrowth (SIBO) in MS patients compared with sex and age matched controls without MS. METHODS:T...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2016.11.004

    authors： Zhang Y,Liu G,Duan Y,Han X,Dong H,Geng J

    更新日期：2016-12-15 00:00:00

  • Citrullinated myelin basic protein induces experimental autoimmune encephalomyelitis in Lewis rats through a diverse T cell repertoire.

    abstract：:An increased proportion of citrullinated MBP (MBP-C8) occurs in the brains of multiple sclerosis (MS) patients. In this study, MBP-C8 from guinea pig (GP) brains was isolated and found encephalitogenic in Lewis rats upon immunization. An encephalitogenic T cell line selected with MBP-C8 preferentially reacted with MBP...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(98)00063-0

    authors： Cao L,Sun D,Whitaker JN

    更新日期：1998-08-01 00:00:00

  • Upregulation of intercellular adhesion molecule-1 (ICAM-1) expression in primary cultures of human brain microvessel endothelial cells by cytokines and lipopolysaccharide.

    abstract：:The expression of intercellular adhesion molecule-1 (ICAM-1) by human cerebral endothelium was studied in primary cultures of human brain microvessel endothelial cells following treatment with bacterial lipopolysaccharide (LPS), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and interferon-gam...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(92)90170-p

    authors： Wong D,Dorovini-Zis K

    更新日期：1992-07-01 00:00:00

  • Effects of dexamethasone on microglial activation in vivo: selective downregulation of major histocompatibility complex class II expression in regenerating facial nucleus.

    abstract：:Following axotomy of the facial nerve microglial cells in the facial nucleus become activated, proliferate, and newly express class I and class II major histocompatibility complex (MHC) antigens. Dexamethasone treatment, starting 2 days prior to axotomy at 1 mg/kg/day, selectively inhibited axotomy-induced MHC class I...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(91)90119-r

    authors： Kiefer R,Kreutzberg GW

    更新日期：1991-11-01 00:00:00

  • Identification and functional characterization of a highly polymorphic region in the human TRAIL promoter in multiple sclerosis.

    abstract：:TNF-related apoptosis-inducing ligand (TRAIL) is not only involved in cell death but also in other immunoregulatory mechanisms. So far, the regulation of the TRAIL pathway in physiologic and pathologic conditions remains unclear. Due to the implication in brain damage and the elevated expression in peripheral immune c...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2003.12.014

    authors： Weber A,Wandinger KP,Mueller W,Aktas O,Wengert O,Grundström E,Ehrlich S,Windemuth C,Kuhlmann T,Wienker T,Brück W,Zipp F

    更新日期：2004-04-01 00:00:00

  • Brain-derived neurotrophic factor expression increases after enzyme replacement therapy in Gaucher disease.

    abstract：:Mutations in the GBA gene are related to an increased risk of developing neurodegenerative diseases. The exact molecular mechanisms involved in the interaction between GBA and α-synuclein, a protein that has been associated with several neurological diseases, remain unsolved. Brain-derived neurotrophic factor (BDNF) i...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2014.11.005

    authors： Vairo F,Sperb-Ludwig F,Wilke M,Michellin-Tirelli K,Netto C,Neto EC,Doederlein Schwartz IV

    更新日期：2015-01-15 00:00:00

  • Muscarinic cholinergic receptor subtypes in the pigeon bursa of Fabricius: a radioligand binding and autoradiographic study.

    abstract：:The pharmacological profile and the anatomical localisation of muscarinic cholinergic receptor subtypes were studied in the pigeon bursa of Fabricius, using radioligand binding and autoradiographic techniques with [3H]quinuclidinyl benzilate (QNB) as a ligand. [3H]QNB was specifically bound to sections of bursa of Fab...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(96)00012-4

    authors： Ricci A,Bronzetti E,Felici L,Ciriaco E,Vega JA,Germanà G

    更新日期：1996-05-01 00:00:00

  • Deficient p75 low-affinity neurotrophin receptor expression does alter the composition of cellular infiltrate in experimental autoimmune encephalomyelitis in C57BL/6 mice.

    abstract：:We have shown earlier that induction of experimental autoimmune encephalomyelitis (EAE)-a model for the human disease multiple sclerosis-in C57BL/6 wild-type mice resulted in the expression of the p75 low-affinity neurotrophin receptor (p75NTR) in endothelial cells in the CNS. In comparison to the clinical manifestati...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2006.01.020

    authors： Küst B,Mantingh-Otter I,Boddeke E,Copray S

    更新日期：2006-05-01 00:00:00

  • Humoral immunity in the central nervous system of Lewis rats infected with Borna disease virus.

    abstract：:The aim of this study was to investigate the humoral immune response to Borna disease (BD) virus in the brain of experimentally infected Lewis rats. Abundant IgG was detected in BD-rat brain with isotype variation throughout infection. IgG was locally produced as indicated by an intact blood-brain barrier, Ig kappa li...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(98)00066-6

    authors： Hatalski CG,Hickey WF,Lipkin WI

    更新日期：1998-10-01 00:00:00

  • Cerebrospinal fluid oligoclonal IgM bands predict early conversion to clinically definite multiple sclerosis in patients with clinically isolated syndrome.

    abstract：:We reviewed the records of 391 patients who had presented with a Clinically Isolated Syndrome and selected 205 who had performed a baseline spinal tap and MRI scan. We studied cerebrospinal fluid (CSF) and serum IgM oligoclonal bands (IgMOB) using agarose gel isoelectric focusing and analyzed the impact of baseline cl...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2013.01.011

    authors： Ferraro D,Simone AM,Bedin R,Galli V,Vitetta F,Federzoni L,D'Amico R,Merelli E,Nichelli PF,Sola P

    更新日期：2013-04-15 00:00:00

  • Studies on the intrathecal humoral immune response in canine distemper encephalitis.

    abstract：:Albumin and IgG were quantitated in paired cerebrospinal fluid (CSF) and serum samples from dogs with demyelinating canine distemper virus (CDV) infection by means of rocket immunoelectrophoresis. The IgG index as indicator for intrathecal immunoglobulin synthesis was normal in animals with non-inflammatory demyelinat...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(86)90073-1

    authors： Vandevelde M,Zurbriggen A,Steck A,Bichsel P

    更新日期：1986-03-01 00:00:00

  • A double-label flow cytometric analysis of the simultaneous expression of OKT4 and Leu2a antigens on circulating T lymphocytes in myasthenia gravis.

    abstract：:A two-color immunofluorescence analysis of circulating abnormal T lymphocytes bearing both helper (T4) and suppressor/cytotoxic (Leu2a) T cell markers (double marker cells) was performed by means of the laser flow cytometry system. The double marker cell level was very low in 6 patients with multiple sclerosis and 12 ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/0165-5728(86)90084-6

    authors： Matsui M,Kameyama M

    更新日期：1986-06-01 00:00:00

  • Proteomic analysis of rat tibialis anterior muscles at different stages of experimental autoimmune myasthenia gravis.

    abstract：:Myasthenia gravis (MG) is an autoimmune disease in which autoantibodies, most commonly directed against the acetylcholine receptor (AChR), impair neuromuscular transmission and cause muscle weakness. In this study, we utilized two-dimensional difference in-gel electrophoresis (2D-DIGE) to analyze the muscle's proteomi...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2013.05.008

    authors： Gomez AM,Vanheel A,Losen M,Molenaar PC,De Baets MH,Noben JP,Hellings N,Martinez-Martinez P

    更新日期：2013-08-15 00:00:00

  • Interferon-γ potentiates GABAA receptor-mediated inhibitory currents in rat hippocampal CA1 pyramidal neurons.

    abstract：:The neural transmission and plasticity can be differentially modulated by various elements of the immune system. Interferon-γ (IFN-γ) is a "pro-inflammatory" cytokine mainly produced by T lymphocytes, activates its corresponding receptor and plays important roles under both homeostatic and inflammatory conditions. How...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2019.577050

    authors： Flood L,Korol SV,Ekselius L,Birnir B,Jin Z

    更新日期：2019-12-15 00:00:00

  • The neuropeptides α-MSH and NPY modulate phagocytosis and phagolysosome activation in RAW 264.7 cells.

    abstract：:Within the immunosuppressive ocular microenvironment, there are constitutively present the immunomodulating neuropeptides alpha-melanocyte stimulating hormone (α-MSH) and neuropeptide Y (NPY) that promote suppressor functionality in macrophages. In this study, we examined the possibility that α-MSH and NPY modulate ph...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2013.04.019

    authors： Phan TA,Taylor AW

    更新日期：2013-07-15 00:00:00

  • Cyclophilin inhibitor NIM811 ameliorates experimental allergic encephalomyelitis.

    abstract：:Cyclophilins have diverse functions that may affect the course of central nervous system (CNS) inflammatory disorders. Anti-inflammatory and neuroprotective mechanisms may be targeted by inhibition of cyclophilin A-dependent and cyclophilin D-dependent functions, respectively. We tested the effect of cyclophilin inhib...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2017.07.016

    authors： Huang ZL,Pandya D,Banta DK,Ansari MS,Oh U

    更新日期：2017-10-15 00:00:00

  • Effects of leptin, ghrelin and neuropeptide y on spike-wave discharge activity and certain biochemical parameters in WAG/Rij rats with genetic absence epilepsy.

    abstract：:This study aimed to evaluate the effects of leptin, ghrelin and neuropeptide-Y on the development of nonconvulsive seizure activity and their role on combating oxidative stress and cytokines produced by the systemic immune response in the WAG/Rij rat model for genetic absence epilepsy. Current study showed that all th...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2020.577454

    authors： Oztas B,Sahin D,Kir H,Kuskay S,Ates N

    更新日期：2021-02-15 00:00:00

  • Combination treatment of celecoxib and ciprofloxacin attenuates live S. aureus induced oxidative damage and inflammation in murine microglia via regulation of cytokine balance.

    abstract：:Microglial activation is the most common phenomenon in S. aureus induced brain abscesses as well as other common neurodegenerative diseases. The main objective of this study is to reduce the microglial inflammation with effective bacterial elimination. Ciprofloxacin and celecoxib were used in combination to regulate S...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2017.12.006

    authors： Dey R,Sultana S,Bishayi B

    更新日期：2018-03-15 00:00:00

  • Suppression of experimental autoimmune encephalomyelitis (EAE) by intraperitoneal administration of soluble myelin antigens in Wistar rats.

    abstract：:Intraperitoneal (i.p.) treatment of Wistar rats with bovine myelin (BM) or myelin basic protein (MBP) previously to immunization with BM-CFA showed a diminished incidence and severity of experimental autoimmune encephalomyelitis (EAE) (2/13 and 0/7, respectively) when compared with rats immunized with BM-CFA (11/17) o...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(96)00117-8

    authors： Rivero VE,Maccioni M,Bucher AE,Roth GA,Riera CM

    更新日期：1997-01-01 00:00:00

  • Immunological patterns identifying disease course and evolution in multiple sclerosis patients.

    abstract：:Reliable, and easy to measure, immunological markers able to denote disease characteristics in multiple sclerosis (MS) patients are still lacking. We applied a multivariate statistical analysis on results obtained by measuring-by real-time RT-PCR-mRNA levels of 25 immunological relevant molecules in PBMCs from 198 MS ...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2005.04.012

    authors： Furlan R,Rovaris M,Martinelli Boneschi F,Khademi M,Bergami A,Gironi M,Deleidi M,Agosta F,Franciotta D,Scarpini E,Uccelli A,Zaffaroni M,Kurne A,Comi G,Olsson T,Filippi M,Martino G

    更新日期：2005-08-01 00:00:00

  • Transient increase in cytokines and nerve growth factor in the rat dorsal root ganglia after nerve lesion and peripheral inflammation.

    abstract：:Inflammatory response occurs, in general, at the peripheral site of injury or irritation and in the corresponding spinal dorsal horn segments. In this study, we show transient increases in the protein concentrations of interleukins 1beta, 6, 8 and NGF in lumbar DRGs within hours after sciatic mononeuropathy in the rat...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2009.01.011

    authors： Saab CY,Shamaa F,El Sabban ME,Safieh-Garabedian B,Jabbur SJ,Saadé NE

    更新日期：2009-03-31 00:00:00

  • Intracerebroventricular interleukin-1beta impairs clearance of tumor cells from the lungs: role of brain prostaglandins.

    abstract：:We have previously demonstrated that central administration of interleukin (IL)-1beta suppresses natural killer (NK) cell activity, impairs NK-mediated lung clearance of tumor cells, and enhances tumor colonization. The central pathways activated by IL-1beta are as yet unknown. Using an in vivo model of tumor coloniza...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/s0165-5728(01)00369-1

    authors： Hodgson DM,Yirmiya R,Taylor AN

    更新日期：2001-09-03 00:00:00

  • Regional difference in inflammatory response to LPS-injection in the brain: role of microglia cell density.

    abstract：:To elucidate whether density of cells could contribute to the extent of microglial activation, we performed in vitro assays using three different densities of N13 microglia stimulated with LPS. Our results showed that induction of pro-inflammatory factors as TNF-α and iNOS was directly related to cell density, meanwhi...

    journal_title：Journal of neuroimmunology

    pub_type： 杂志文章

    doi：10.1016/j.jneuroim.2011.06.017

    authors： Pintado C,Revilla E,Vizuete ML,Jiménez S,García-Cuervo L,Vitorica J,Ruano D,Castaño A

    更新日期：2011-09-15 00:00:00