Abstract:
:A protein microarray based on DNA microarray platform was developed to identify protein-protein interactions in vitro. The conventional DNA chip surface by 156-bp PCR product was prepared for a substrate of protein microarray. High-affinity sequence-specific DNA binding domain, GAL4 DNA binding domain, was introduced to the protein microarray as fusion partner of a target model protein, enhanced green fluorescent protein. The target protein was oriented immobilized directly on the DNA chip surface. Finally, monoclonal antibody of the target protein was used to identify the immobilized protein on the surface. This study shows that the conventional DNA chip can be used to make a protein microarray directly, and this novel protein microarray can be applicable as a tool for identifying protein-protein interactions.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Choi YS,Pack SP,Yoo YJdoi
10.1016/j.bbrc.2005.01.167subject
Has Abstractpub_date
2005-04-22 00:00:00pages
1315-9issue
4eissn
0006-291Xissn
1090-2104pii
S0006-291X(05)00207-Xjournal_volume
329pub_type
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