Abstract:
:Previous studies have shown that DNA strand breaks are an early consequence of nitric oxide toxicity in pancreatic islet cells. We show here that exposure of islet cells to chemical NO donors causes the formation of ADP-ribose polymers in cell nuclei, with concomitant depletion of intracellular NAD+. Islet cell lysis was largely prevented by the ADP-ribosylation inhibitors nicotinamide, 3-aminobenzamide, and 4-amino-1,8-naphthalimide, the latter being a potent new-generation compound with high selectivity for poly(ADP-ribosyl)-ation. These findings indicate a key role of poly(ADP-ribose) polymerase activation in NO toxicity in islet cells.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Radons J,Heller B,Bürkle A,Hartmann B,Rodriguez ML,Kröncke KD,Burkart V,Kolb Hdoi
10.1006/bbrc.1994.1368subject
Has Abstractpub_date
1994-03-30 00:00:00pages
1270-7issue
3eissn
0006-291Xissn
1090-2104pii
S0006-291X(84)71368-4journal_volume
199pub_type
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