Abstract:
:The fifth component of the complement cascade, C5a, was iodinated using the Bolton-Hunter reagent. Results from the present study, using the high affinity ligand, [125I]Bolton-Hunter-labeled C5a ([125I]BH-C5a), revealed a single binding site on membranes prepared from human neutrophils, U-937 cells and human monocytes. Saturation studies demonstrated Bmax values in these cells of 11.5, 47.3 and 16.6 fmol/10(6) cells, respectively. The C5a receptor demonstrated a very high affinity for [125I]BH-C5a of approximately 4 pM in each cell type. Competition studies using analogs of C5a generated a similar order of potency in each of the cell types of C5a > or = C5a(1-74), Ser66Ala > C5a(1-73) > C5a(1-69). These studies indicate that [125I]BH-C5a labels a similar receptor in neutrophil, U-937 cell and monocyte membranes. Furthermore, C5a(1-73) produced shallow inhibition curves in competition experiments in each cell type. Computer analysis of the binding data revealed two components of binding. When 10 nM unlabeled C5a was used to initiate dissociation of [125I]BH-C5a binding in neutrophil membranes, two binding components were observed. In addition, dissociation of [125I]BH-C5a binding by 10 nM unlabeled C5a in the presence of 1 mM GppNHp decreased the percentage of binding to the slowly dissociating, high affinity binding component from 84 to 58%. These results suggest that multiple states of the C5a receptor exist.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Braunwalder AF,Musmanno D,Galakatos N,Garlick RH,Haston WO,Rediske JJ,Wennogle L,Seligmann B,Sills MAdoi
10.1016/0161-5890(92)90168-wsubject
Has Abstractpub_date
1992-11-01 00:00:00pages
1319-24issue
11eissn
0161-5890issn
1872-9142journal_volume
29pub_type
杂志文章abstract::It is well established that aggregation of cell surface immunoglobulin is involved in signal transduction by cells of the immune system. It is less well understood what special properties of these cell surface aggregates are important in initiating the signal cascade. Several authors have proposed that cells respond t...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(94)90160-0
更新日期:1994-12-01 00:00:00
abstract::We have previously developed a number of monoclonal antibodies (Mabs) that bind to protamine. One of these antibodies, Hup1N, binds to human protamine 1 but not to protamine 2. In contrast, Mab HupA binds both protamine 1 and protamine 2. The epitopes for these two Mabs were observed to overlap, and were localized to ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90436-f
更新日期:1993-12-01 00:00:00
abstract::Arginine kinase (AK) is a panallergen present in crustaceans, which can induce an immunoglobulin (Ig) E-mediated immune response in humans. The aim of this work was to map and characterize the antigenic epitopes of Scylla paramamosain AK. Specific-protein-A-enriched IgG raised in rabbits against purified S. paramamosa...
journal_title:Molecular immunology
pub_type: 临床试验,杂志文章
doi:10.1016/j.molimm.2015.02.010
更新日期:2015-06-01 00:00:00
abstract::In periodontal disease, IgG1 and IgA1 antibodies produced in situ deposit on antigens in the affected tissues. Thus, there is an interest in the effect of co-deposited IgA1 antibodies on complement activation by IgG1-immune complexes. In the present study, we first analyzed the effect of IgA1-immune complexes on compl...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.02.004
更新日期:2006-02-01 00:00:00
abstract::The ragweed allergens Amb t 5 and Amb a 5 are among the smallest inhaled protein allergens known, containing a single, immunodominant T-cell epitope. In this study we analyzed the B-cell epitope structure of Amb t 5. The three-dimensional structures of Amb t 5 and Amb a 5 have been determined by NMR spectroscopy, prov...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/s0161-5890(99)00054-1
更新日期:1998-06-01 00:00:00
abstract::IL-10 plays a critical role in controlling inflammation and the anti-inflammatory functions of IL-10 are regulated based on its coordinated expression from various cellular sources, most notably T cells. Although nearly all CD4+ subpopulations can express IL-10, surprisingly little is known about the molecular mechani...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.09.009
更新日期:2010-11-01 00:00:00
abstract::Dendritic cells (DCs) serve to maintain peripheral tolerance under steady state conditions. Upon triggering by activation signals they initiate strong immune responses. The activation of DCs is accompanied by a rapid upregulation of proinflammatory cytokines, which were shown in other cell types to be regulated by mec...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.11.002
更新日期:2010-02-01 00:00:00
abstract::Previous studies showed that P2 receptors are involved in neutrophil migration via stimulation of chemokine release and by facilitating chemoattractant gradient sensing. Here, we have investigated whether these receptors are involved in LPS-induced neutrophil transendothelial migration (TEM) using a Boyden chamber whe...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2009.11.020
更新日期:2010-02-01 00:00:00
abstract::The translation of mRNA within total RNA of German (Blattella germanica) cockroaches was performed using a rabbit reticulocyte lysate system. Analysis of the translation products by SDS-PAGE and combined autoradiography revealed several synthesized proteins with apparent molecular weights ranging from 20 kD to 110 kD....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90443-f
更新日期:1993-12-01 00:00:00
abstract::Pathogen-driven selection is believed to be important in the evolution and maintenance of the polymorphism of the major histocompatibility complex (MHC) genes but have been tested for very few vertebrates. In this study, we first investigate by SSCP (single strand conformational polymorphism) the diversity found at th...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2008.03.007
更新日期:2008-06-01 00:00:00
abstract::The innate immune response is critical to enteric disease resistance and the induction of mucosal adaptive immunity. In mucosae of the small intestine, Peyer's patches play a central role in immune surveillance and sampling of bacteria by specialized M cells. The innate immune response to Salmonella enterica serovar C...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.10.011
更新日期:2006-04-01 00:00:00
abstract::Type 1 diabetes (T1D) is a chronic disease characterized by autoimmune-mediated destruction of pancreatic insulin-producing beta cells. Interleukin (IL)-18 is a pro-inflammatory cytokine implicated in the pathogenesis of a number of inflammatory diseases. Here, we analyzed IL-18 levels in the plasma of juveniles with ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2014.12.012
更新日期:2015-04-01 00:00:00
abstract::A variety of important cellular functions are regulated by cytokines. The Jak-STAT pathway is one of the important signaling pathways downstream of cytokine receptors. Following binding of a ligand to its cognate receptor, receptor-associated Jaks are activated. STAT proteins are then in turn activated by tyrosine pho...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/s0161-5890(00)00018-3
更新日期:2000-01-01 00:00:00
abstract::Malignant cells expressing NKG2D ligands on their cell surface can be directly sensed and killed by NKG2D-bearing lymphocytes. To ensure this immune recognition, accumulating evidence suggests that NKG2D ligands are trafficed via alternative pathways to the cell surface. We have previously shown that the NKG2D ligand ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2015.04.022
更新日期:2015-08-01 00:00:00
abstract::The primary structure of a human mu heavy chain (DAG) protein is described. The native protein is a circular decamer with a molecular weight (Mr) of 500 kDa, each decamer being constituted of the constant domains C mu 2, C mu 3 and C mu4 and interlinked by 15 disulfide bridges. At its NH2-terminal each monomeric chain...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(90)90086-f
更新日期:1990-08-01 00:00:00
abstract:BACKGROUND:Individuals genetically deficient of properdin are more susceptible to meningococcal disease. Likewise low concentration or decreased biological activity of mannose-binding lectin (MBL) is associated with higher incidence of bacterial infections during childhood. In this study we report our findings in a Dan...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.02.017
更新日期:2006-02-01 00:00:00
abstract::Dendritic cells (DCs) are suspected to be involved in the development of atherogenesis, but their role is still unclear. The aim of this study was to characterize circulating DCs and monocyte-derived DCs (Mo-DCs) of obese and diabetic patients (T2D), and to study their interaction with human coronary smooth muscle cel...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2011.08.019
更新日期:2011-10-01 00:00:00
abstract::Triptolide is an active component of extracts derived from the medicinal vine Tripterygium Wilfordii Hook. f. (TWHF) whose extracts have been used to treat inflammatory bowel disease (IBD). We have reported that triptolide showed therapeutic activity in a murine IBD model, but the potential mechanism of action of this...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.06.007
更新日期:2010-09-01 00:00:00
abstract::Recent reports demonstrate that natural killer (NK) cells and dendritic cells (DC) support each other's activity in a positive feedback. We observed that activated NK cells induce the maturation of DCs into stable type-1 polarized DCs (DC1), characterized by up to 100-fold enhanced ability to produce IL-12p70 in respo...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2004.07.038
更新日期:2005-02-01 00:00:00
abstract::Cleavage by the proteasome is responsible for generating the C terminus of T-cell epitopes. Modeling the process of proteasome cleavage as part of a multi-step algorithm for T-cell epitope prediction will reduce the number of non-binders and increase the overall accuracy of the predictive algorithm. Quantitative matri...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2005.12.013
更新日期:2006-05-01 00:00:00
abstract::Whether bovine myelin basic protein (BP) and bovine spinal cord protein (SCP) cross-react at the humoral immune level was assessed with a sensitive solid-phase enzyme immunoassay. We found that a hyperimmune anti-SCP serum reacted strongly with SCP and cross-reacted nearly as well with BP. A hyperimmune anti-BP serum ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90159-7
更新日期:1984-10-01 00:00:00
abstract::Anaerobic reduction of purified rabbit IgG antibody (Ab) with 1.5 moles of dithiothreitol per mole of Ab at pH 8.0, followed by alkylation, cleaves 39% of the inter-heavy-chain (H-H) disulfide (SS) bonds. This treatment has the following effects on the ability of the Ab to activate the classical pathway of complement....
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(84)90092-0
更新日期:1984-01-01 00:00:00
abstract::The innate immune system is the first line of defense against infection and responses are initiated by pattern recognition receptors (PRRs) that detect pathogen-associated molecular patterns (PAMPs). PRRs also detect endogenous danger-associated molecular patterns (DAMPs) that are released by damaged or dying cells. T...
journal_title:Molecular immunology
pub_type: 杂志文章,评审
doi:10.1016/j.molimm.2017.02.012
更新日期:2017-06-01 00:00:00
abstract::Viral myocarditis (VMC) is an inflammation of the myocardium closely associated with Coxsackievirus B3 (CVB3) infection. Vγ1+γδT cells, one of early cardiac infiltrated innate population, were reported to protect CVB3 myocarditis while the precise mechanism not fully addressed. To explore cytokine profiles and kinetic...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.11.006
更新日期:2017-01-01 00:00:00
abstract::A number of fragments derived from acid-treated rabbit IgG by digestion with plasmin have been separated by high-resolution gel filtration. Fragments isolated included a dimer and monomer Facb, named F(acb)2 and Facb, respectively and a heterodimer composed of Facb and Fab subunits, named F(acb)(ab). A C gamma 2 fragm...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(85)90147-6
更新日期:1985-07-01 00:00:00
abstract::The immunoregulatory enzyme indoleamine 2,3-dioxygenase (IDO) suppresses T-cell responses and promotes immune tolerance in tumor resistance. A previous study determined that IDO inhibits Vav1 mRNA expression and the activation of Vav1 and its downstream targets in T cells in the guanine exchange factor (GEF)-independe...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2016.11.018
更新日期:2017-01-01 00:00:00
abstract::The high affinity receptor for IgE, which mediates allergic reactions, is found exclusively on mast cells, basophils and epidermal Langerhans cells. It is composed of an alpha, beta and two gamma-polypeptide chains, inserted as a complex into the plasma membrane. The alpha and beta-subunits are produced only by these ...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(96)84614-1
更新日期:1996-07-01 00:00:00
abstract::Intercellular adhesion molecule-1 (ICAM-1) functions in leukocyte trafficking, activation, and the formation of the immunological synapse. ICAM-1 is a member of the immunoglobulin superfamily of adhesion proteins, which share a similar structure of repeating Ig-like domains. Many genes in this family, including ICAM-1...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2010.03.005
更新日期:2010-05-01 00:00:00
abstract::Tumor necrosis factor-alpha (TNF) has been implicated in the pathogenesis of a variety of human diseases including septic shock, cachexia, graft-versus-host disease and several autoimmune diseases. Monoclonal antibodies directed against TNF provide an attractive mode of therapeutic intervention in these diseases. We h...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/0161-5890(93)90106-l
更新日期:1993-11-01 00:00:00
abstract:BACKGROUND:Tumor necrosis factor-α (TNF-α) is involved in inflammatory responses in atherosclerosis. We propose an in vitro cellular assay to evaluate the anti-inflammatory mechanisms of potential modifiers such as food extracts. In the current model we assessed an anti-inflammatory effect of polyphenol-rich peanut ext...
journal_title:Molecular immunology
pub_type: 杂志文章
doi:10.1016/j.molimm.2012.05.007
更新日期:2012-10-01 00:00:00