Abstract：
:Previous studies showed that P2 receptors are involved in neutrophil migration via stimulation of chemokine release and by facilitating chemoattractant gradient sensing. Here, we have investigated whether these receptors are involved in LPS-induced neutrophil transendothelial migration (TEM) using a Boyden chamber where neutrophils migrated through a layer of lipopolysaccharide (LPS)-stimulated human umbilical vein endothelial cells (HUVECs). In line with a role of P2 receptors, neutrophil TEM was inhibited by the P2 receptor antagonists suramin and reactive blue 2 (RB-2) acting on the basolateral, but not luminal, HUVECs' P2 receptors. HUVECs express P2Y(1), P2Y(2), P2Y(4), P2Y(6) and P2Y(11). The involvement of P2Y(4) was unlikely as this receptor is insensitive to suramin while P2Y(1), P2Y(6) and P2Y(11) were excluded with available selective antagonists, leaving P2Y(2) as the only candidate. Indeed, the P2Y(2) knockdown in HUVECs inhibited neutrophil TEM compared to control HUVECs transfected with scrambled siRNA. Moreover, UTP, a P2Y(2) ligand, markedly potentiated LPS-induced TEM. Interestingly, IL-8 and ICAM-1 had a modest effect on neutrophil TEM in this 3h assay which was significantly diminished by the inhibition of Rho kinase in HUVECs with Y27632. In summary, endothelial P2Y(2) receptors control the early LPS-induced neutrophil TEM in vitro via Rho kinase activation.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Kukulski F,Ben Yebdri F,Bahrami F,Fausther M,Tremblay A,Sévigny Jdoi
10.1016/j.molimm.2009.11.020subject
Has Abstractpub_date
2010-02-01 00:00:00pages
991-9issue
5eissn
0161-5890issn
1872-9142pii
S0161-5890(09)00847-5journal_volume
47pub_type
杂志文章abstract：:Previously we reported that, early in the antibody response of BALB/c mice to several cytochromes c (CYT) coupled to ovalbumin (OVA), B cells responding to the self antigen mouse CYT recognized a single site on mouse CYT and were in much higher frequency than B cells responding to foreign CYT. In the present study the...
journal_title：Molecular immunology
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更新日期：2014-06-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章,评审
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更新日期：2005-05-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2002-10-01 00:00:00
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更新日期：2019-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：1988-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：2008-04-01 00:00:00
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更新日期：2021-02-01 00:00:00
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pub_type： 杂志文章
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更新日期：1996-09-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2016-04-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：1989-01-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
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更新日期：2008-03-01 00:00:00
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journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(82)90281-4
更新日期：1982-12-01 00:00:00
